Lem3 cdc50 family protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

LEM3 (ligand-effect modulator 3) family / CDC50 family, putative

Species:

Leishmania mexicana

UniProt:

E9B2H4_LEIMU

TriTrypDb:

LmxM.31.0510

Length:

363

Annotations

LeishMANIAdb annotations

Homologous to animal CDC50 family phospholipid flippase (ATPase) proteins.. This family has expanded in kinetoplastids

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 10

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 20
NetGPI	no	yes: 0, no: 20

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	21
GO:0110165	cellular anatomical entity	1	21
GO:0005783	endoplasmic reticulum	5	1
GO:0005794	Golgi apparatus	5	1
GO:0005886	plasma membrane	3	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1

Expansion

Sequence features

E9B2H4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B2H4

Function

Biological processes
Term	Name	Level	Count
GO:0033036	macromolecule localization	2	21
GO:0051179	localization	1	21

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	214	216	PF00675	0.493
CLV_PCSK_SKI1_1	117	121	PF00082	0.410
CLV_PCSK_SKI1_1	124	128	PF00082	0.486
CLV_PCSK_SKI1_1	200	204	PF00082	0.544
CLV_PCSK_SKI1_1	207	211	PF00082	0.517
CLV_PCSK_SKI1_1	242	246	PF00082	0.373
DOC_CYCLIN_yCln2_LP_2	106	112	PF00134	0.215
DOC_CYCLIN_yCln2_LP_2	41	47	PF00134	0.188
DOC_MAPK_FxFP_2	149	152	PF00069	0.275
DOC_MAPK_gen_1	284	293	PF00069	0.261
DOC_MAPK_MEF2A_6	284	293	PF00069	0.252
DOC_PP1_RVXF_1	56	62	PF00149	0.218
DOC_PP2B_LxvP_1	106	109	PF13499	0.243
DOC_PP2B_LxvP_1	41	44	PF13499	0.207
DOC_PP4_FxxP_1	149	152	PF00568	0.221
DOC_PP4_FxxP_1	223	226	PF00568	0.146
DOC_USP7_MATH_1	214	218	PF00917	0.276
DOC_USP7_MATH_1	310	314	PF00917	0.337
DOC_USP7_UBL2_3	216	220	PF12436	0.212
DOC_USP7_UBL2_3	7	11	PF12436	0.661
DOC_WW_Pin1_4	24	29	PF00397	0.514
LIG_14-3-3_CanoR_1	124	132	PF00244	0.283
LIG_14-3-3_CanoR_1	138	142	PF00244	0.258
LIG_14-3-3_CanoR_1	215	223	PF00244	0.305
LIG_14-3-3_CterR_2	358	363	PF00244	0.600
LIG_BIR_II_1	1	5	PF00653	0.610
LIG_BRCT_BRCA1_1	31	35	PF00533	0.390
LIG_BRCT_BRCA1_1	312	316	PF00533	0.272
LIG_EH1_1	34	42	PF00400	0.207
LIG_FHA_1	105	111	PF00498	0.279
LIG_FHA_1	125	131	PF00498	0.102
LIG_FHA_1	277	283	PF00498	0.310
LIG_FHA_1	340	346	PF00498	0.333
LIG_FHA_1	54	60	PF00498	0.336
LIG_FHA_2	55	61	PF00498	0.254
LIG_GBD_Chelix_1	33	41	PF00786	0.232
LIG_LIR_Apic_2	221	226	PF02991	0.233
LIG_LIR_Gen_1	129	139	PF02991	0.290
LIG_LIR_Gen_1	313	320	PF02991	0.311
LIG_LIR_Gen_1	60	71	PF02991	0.267
LIG_LIR_Nem_3	129	135	PF02991	0.297
LIG_LIR_Nem_3	283	289	PF02991	0.247
LIG_LIR_Nem_3	297	302	PF02991	0.198
LIG_LIR_Nem_3	313	319	PF02991	0.304
LIG_LIR_Nem_3	32	38	PF02991	0.325
LIG_LIR_Nem_3	60	66	PF02991	0.233
LIG_MYND_1	226	230	PF01753	0.120
LIG_MYND_2	225	229	PF01753	0.120
LIG_NRBOX	331	337	PF00104	0.417
LIG_PTAP_UEV_1	306	311	PF05743	0.218
LIG_PTB_Apo_2	119	126	PF02174	0.254
LIG_PTB_Apo_2	65	72	PF02174	0.292
LIG_PTB_Phospho_1	119	125	PF10480	0.218
LIG_SH2_CRK	132	136	PF00017	0.284
LIG_SH2_CRK	286	290	PF00017	0.243
LIG_SH2_GRB2like	163	166	PF00017	0.254
LIG_SH2_NCK_1	63	67	PF00017	0.263
LIG_SH2_SRC	255	258	PF00017	0.189
LIG_SH2_SRC	63	66	PF00017	0.234
LIG_SH2_STAT3	253	256	PF00017	0.120
LIG_SH2_STAT3	311	314	PF00017	0.182
LIG_SH2_STAT5	125	128	PF00017	0.267
LIG_SH2_STAT5	248	251	PF00017	0.273
LIG_SH2_STAT5	299	302	PF00017	0.217
LIG_SH3_3	19	25	PF00018	0.547
LIG_SH3_3	2	8	PF00018	0.575
LIG_SH3_3	288	294	PF00018	0.290
LIG_SH3_3	304	310	PF00018	0.290
LIG_SH3_3	78	84	PF00018	0.306
LIG_SH3_4	7	14	PF00018	0.591
LIG_SH3_5	294	298	PF00018	0.290
LIG_SUMO_SIM_anti_2	30	35	PF11976	0.333
LIG_SUMO_SIM_anti_2	334	340	PF11976	0.427
LIG_SUMO_SIM_par_1	27	32	PF11976	0.530
LIG_SUMO_SIM_par_1	46	52	PF11976	0.145
LIG_TRFH_1	114	118	PF08558	0.408
LIG_TYR_ITIM	130	135	PF00017	0.442
LIG_WW_3	224	228	PF00397	0.252
MOD_CK1_1	140	146	PF00069	0.372
MOD_CK1_1	27	33	PF00069	0.444
MOD_CK1_1	277	283	PF00069	0.273
MOD_GlcNHglycan	135	138	PF01048	0.286
MOD_GlcNHglycan	210	213	PF01048	0.374
MOD_GlcNHglycan	236	239	PF01048	0.404
MOD_GlcNHglycan	241	245	PF01048	0.410
MOD_GlcNHglycan	91	94	PF01048	0.402
MOD_GSK3_1	133	140	PF00069	0.265
MOD_GSK3_1	276	283	PF00069	0.416
MOD_GSK3_1	49	56	PF00069	0.420
MOD_GSK3_1	85	92	PF00069	0.287
MOD_GSK3_1	98	105	PF00069	0.218
MOD_N-GLC_1	175	180	PF02516	0.240
MOD_N-GLC_1	85	90	PF02516	0.272
MOD_N-GLC_2	206	208	PF02516	0.246
MOD_NEK2_1	100	105	PF00069	0.165
MOD_NEK2_1	175	180	PF00069	0.240
MOD_NEK2_1	184	189	PF00069	0.240
MOD_NEK2_1	29	34	PF00069	0.372
MOD_NEK2_1	339	344	PF00069	0.248
MOD_NEK2_1	346	351	PF00069	0.239
MOD_NEK2_1	85	90	PF00069	0.319
MOD_PIKK_1	100	106	PF00454	0.189
MOD_PIKK_1	310	316	PF00454	0.413
MOD_PKA_2	137	143	PF00069	0.319
MOD_PKA_2	214	220	PF00069	0.418
MOD_PKA_2	54	60	PF00069	0.340
MOD_Plk_1	175	181	PF00069	0.244
MOD_Plk_1	85	91	PF00069	0.239
MOD_Plk_4	137	143	PF00069	0.292
MOD_Plk_4	184	190	PF00069	0.333
MOD_Plk_4	29	35	PF00069	0.307
MOD_Plk_4	331	337	PF00069	0.356
MOD_Plk_4	346	352	PF00069	0.217
MOD_Plk_4	93	99	PF00069	0.385
MOD_ProDKin_1	24	30	PF00069	0.397
MOD_SUMO_rev_2	201	209	PF00179	0.400
TRG_DiLeu_BaLyEn_6	224	229	PF01217	0.127
TRG_ENDOCYTIC_2	123	126	PF00928	0.304
TRG_ENDOCYTIC_2	132	135	PF00928	0.343
TRG_ENDOCYTIC_2	286	289	PF00928	0.287
TRG_ENDOCYTIC_2	299	302	PF00928	0.244
TRG_ENDOCYTIC_2	63	66	PF00928	0.271
TRG_ER_diArg_1	114	117	PF00400	0.172
TRG_Pf-PMV_PEXEL_1	200	205	PF00026	0.155
TRG_Pf-PMV_PEXEL_1	287	292	PF00026	0.264

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PBG2	Leptomonas seymouri	30%	87%
A0A0N1PDQ9	Leptomonas seymouri	59%	100%
A0A0S4IMK4	Bodo saltans	31%	90%
A0A0S4J4T1	Bodo saltans	38%	96%
A0A0S4JB98	Bodo saltans	31%	90%
A0A0S4JJY0	Bodo saltans	29%	90%
A0A0S4JQR9	Bodo saltans	33%	69%
A0A1X0NHQ5	Trypanosomatidae	32%	76%
A0A1X0NV71	Trypanosomatidae	39%	96%
A0A1X0P5L3	Trypanosomatidae	32%	91%
A0A3R7LIN7	Trypanosoma rangeli	32%	85%
A0A3R7MGX3	Trypanosoma rangeli	42%	97%
A0A3S7X551	Leishmania donovani	85%	100%
A0A3S7X9N4	Leishmania donovani	30%	86%
A0A422NR22	Trypanosoma rangeli	33%	91%
A4HK31	Leishmania braziliensis	69%	100%
A4HN07	Leishmania braziliensis	29%	100%
A4I7N1	Leishmania infantum	85%	100%
A4IBN0	Leishmania infantum	30%	86%
C9ZZ02	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	32%	94%
D0A9K3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	29%	77%
D0A9R9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	38%	100%
E9AFG4	Leishmania major	30%	100%
E9B6L9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	30%	86%
H2L0H3	Caenorhabditis elegans	23%	100%
P25656	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	23%	93%
P42838	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	23%	88%
P53740	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	25%	92%
Q17QL5	Bos taurus	31%	100%
Q1MTQ5	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	27%	92%
Q2T9P5	Bos taurus	25%	100%
Q3MIR4	Homo sapiens	28%	100%
Q4Q5N2	Leishmania major	82%	100%
Q5F362	Gallus gallus	29%	98%
Q5R6C0	Pongo abelii	30%	100%
Q67YS6	Arabidopsis thaliana	25%	100%
Q6AY41	Rattus norvegicus	29%	100%
Q8BHG3	Mus musculus	27%	100%
Q8L8W0	Arabidopsis thaliana	26%	100%
Q8VEK0	Mus musculus	30%	100%
Q95JK4	Macaca fascicularis	24%	100%
Q96WW4	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	27%	98%
Q9D4D7	Mus musculus	23%	100%
Q9LTW0	Arabidopsis thaliana	26%	100%
Q9NV96	Homo sapiens	30%	100%
Q9SA35	Arabidopsis thaliana	26%	100%
Q9SLK2	Arabidopsis thaliana	24%	100%
V5AY40	Trypanosoma cruzi	35%	100%
V5B6A6	Trypanosoma cruzi	43%	96%
V5BY23	Trypanosoma cruzi	33%	91%