tRNA:m(4)X modification enzyme TRM13

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

CCCH zinc finger in TRM13 protein/U11-48K-like CHHC zinc finger/Methyltransferase TRM13, putative

Species:

Leishmania mexicana

UniProt:

E9B1K2_LEIMU

TriTrypDb:

LmxM.30.0520

Length:

564

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

E9B1K2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B1K2

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	1
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006396	RNA processing	6	1
GO:0006399	tRNA metabolic process	7	1
GO:0006400	tRNA modification	6	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008033	tRNA processing	8	1
GO:0008152	metabolic process	1	1
GO:0009451	RNA modification	5	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0030488	tRNA methylation	5	1
GO:0032259	methylation	2	1
GO:0034470	ncRNA processing	7	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034660	ncRNA metabolic process	6	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0043414	macromolecule methylation	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0008168	methyltransferase activity	4	12
GO:0008171	O-methyltransferase activity	5	12
GO:0008173	RNA methyltransferase activity	4	12
GO:0008175	tRNA methyltransferase activity	5	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0043167	ion binding	2	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0062105	RNA 2'-O-methyltransferase activity	5	12
GO:0106050	tRNA 2'-O-methyltransferase activity	6	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140101	catalytic activity, acting on a tRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	14	16	PF00675	0.689
CLV_NRD_NRD_1	153	155	PF00675	0.338
CLV_NRD_NRD_1	169	171	PF00675	0.442
CLV_NRD_NRD_1	357	359	PF00675	0.224
CLV_NRD_NRD_1	37	39	PF00675	0.244
CLV_NRD_NRD_1	515	517	PF00675	0.284
CLV_NRD_NRD_1	519	521	PF00675	0.284
CLV_PCSK_KEX2_1	153	155	PF00082	0.357
CLV_PCSK_KEX2_1	357	359	PF00082	0.217
CLV_PCSK_KEX2_1	515	517	PF00082	0.230
CLV_PCSK_KEX2_1	523	525	PF00082	0.201
CLV_PCSK_PC1ET2_1	523	525	PF00082	0.221
CLV_PCSK_SKI1_1	109	113	PF00082	0.259
CLV_PCSK_SKI1_1	118	122	PF00082	0.399
CLV_PCSK_SKI1_1	316	320	PF00082	0.237
CLV_PCSK_SKI1_1	385	389	PF00082	0.271
CLV_PCSK_SKI1_1	549	553	PF00082	0.242
DEG_APCC_DBOX_1	344	352	PF00400	0.487
DEG_APCC_DBOX_1	384	392	PF00400	0.555
DEG_APCC_DBOX_1	461	469	PF00400	0.442
DEG_SPOP_SBC_1	201	205	PF00917	0.562
DOC_CKS1_1	324	329	PF01111	0.437
DOC_MAPK_gen_1	363	371	PF00069	0.438
DOC_MAPK_gen_1	520	531	PF00069	0.438
DOC_MAPK_MEF2A_6	345	353	PF00069	0.490
DOC_MAPK_MEF2A_6	524	533	PF00069	0.519
DOC_PP1_RVXF_1	528	534	PF00149	0.519
DOC_PP2B_LxvP_1	481	484	PF13499	0.461
DOC_USP7_MATH_1	209	213	PF00917	0.700
DOC_USP7_MATH_1	310	314	PF00917	0.342
DOC_USP7_MATH_1	330	334	PF00917	0.471
DOC_USP7_MATH_1	393	397	PF00917	0.479
DOC_USP7_MATH_1	405	409	PF00917	0.512
DOC_USP7_MATH_1	484	488	PF00917	0.506
DOC_USP7_MATH_1	497	501	PF00917	0.386
DOC_USP7_MATH_1	64	68	PF00917	0.634
DOC_USP7_MATH_1	95	99	PF00917	0.528
DOC_WW_Pin1_4	203	208	PF00397	0.755
DOC_WW_Pin1_4	320	325	PF00397	0.558
DOC_WW_Pin1_4	62	67	PF00397	0.650
DOC_WW_Pin1_4	76	81	PF00397	0.700
LIG_14-3-3_CanoR_1	118	127	PF00244	0.514
LIG_14-3-3_CanoR_1	153	160	PF00244	0.388
LIG_14-3-3_CanoR_1	257	266	PF00244	0.581
LIG_14-3-3_CanoR_1	357	364	PF00244	0.500
LIG_14-3-3_CanoR_1	365	371	PF00244	0.467
LIG_14-3-3_CanoR_1	385	395	PF00244	0.471
LIG_14-3-3_CanoR_1	41	49	PF00244	0.464
LIG_Actin_WH2_2	104	120	PF00022	0.484
LIG_BIR_II_1	1	5	PF00653	0.631
LIG_BIR_III_4	82	86	PF00653	0.430
LIG_Clathr_ClatBox_1	348	352	PF01394	0.460
LIG_FHA_1	119	125	PF00498	0.470
LIG_FHA_1	232	238	PF00498	0.316
LIG_FHA_1	248	254	PF00498	0.536
LIG_FHA_1	320	326	PF00498	0.489
LIG_FHA_1	54	60	PF00498	0.464
LIG_FHA_2	154	160	PF00498	0.444
LIG_FHA_2	299	305	PF00498	0.448
LIG_FHA_2	487	493	PF00498	0.509
LIG_FHA_2	545	551	PF00498	0.484
LIG_HP1_1	347	351	PF01393	0.519
LIG_Integrin_RGD_1	87	89	PF01839	0.518
LIG_LIR_Gen_1	268	275	PF02991	0.390
LIG_LIR_Gen_1	415	424	PF02991	0.430
LIG_LIR_Gen_1	492	501	PF02991	0.417
LIG_LIR_LC3C_4	183	186	PF02991	0.357
LIG_LIR_Nem_3	268	273	PF02991	0.390
LIG_LIR_Nem_3	28	34	PF02991	0.361
LIG_LIR_Nem_3	415	420	PF02991	0.443
LIG_LIR_Nem_3	492	498	PF02991	0.431
LIG_Pex14_1	334	338	PF04695	0.437
LIG_PTAP_UEV_1	326	331	PF05743	0.376
LIG_SH2_SRC	266	269	PF00017	0.199
LIG_SH2_STAP1	103	107	PF00017	0.417
LIG_SH2_STAP1	176	180	PF00017	0.451
LIG_SH2_STAT5	126	129	PF00017	0.403
LIG_SH2_STAT5	155	158	PF00017	0.439
LIG_SH2_STAT5	266	269	PF00017	0.285
LIG_SH2_STAT5	35	38	PF00017	0.431
LIG_SH2_STAT5	53	56	PF00017	0.428
LIG_SH2_STAT5	539	542	PF00017	0.460
LIG_SH2_STAT5	546	549	PF00017	0.438
LIG_SH3_3	17	23	PF00018	0.708
LIG_SH3_3	321	327	PF00018	0.555
LIG_SH3_3	445	451	PF00018	0.504
LIG_SH3_3	465	471	PF00018	0.437
LIG_SH3_3	89	95	PF00018	0.281
LIG_SH3_CIN85_PxpxPR_1	407	412	PF14604	0.394
LIG_SUMO_SIM_anti_2	183	188	PF11976	0.485
LIG_SUMO_SIM_par_1	346	352	PF11976	0.430
LIG_SUMO_SIM_par_1	451	457	PF11976	0.511
LIG_TRAF2_1	220	223	PF00917	0.595
LIG_TRAF2_1	491	494	PF00917	0.507
MOD_CK1_1	212	218	PF00069	0.547
MOD_CK1_1	323	329	PF00069	0.539
MOD_CK1_1	333	339	PF00069	0.554
MOD_CK1_1	434	440	PF00069	0.444
MOD_CK1_1	500	506	PF00069	0.423
MOD_CK1_1	78	84	PF00069	0.690
MOD_CK1_1	98	104	PF00069	0.471
MOD_CK2_1	102	108	PF00069	0.497
MOD_CK2_1	15	21	PF00069	0.718
MOD_CK2_1	298	304	PF00069	0.534
MOD_CK2_1	311	317	PF00069	0.437
MOD_CK2_1	486	492	PF00069	0.433
MOD_Cter_Amidation	518	521	PF01082	0.271
MOD_GlcNHglycan	141	144	PF01048	0.572
MOD_GlcNHglycan	159	163	PF01048	0.431
MOD_GlcNHglycan	197	200	PF01048	0.654
MOD_GlcNHglycan	215	218	PF01048	0.718
MOD_GlcNHglycan	280	283	PF01048	0.309
MOD_GlcNHglycan	327	330	PF01048	0.270
MOD_GlcNHglycan	366	369	PF01048	0.331
MOD_GlcNHglycan	395	398	PF01048	0.277
MOD_GlcNHglycan	407	410	PF01048	0.194
MOD_GSK3_1	145	152	PF00069	0.587
MOD_GSK3_1	203	210	PF00069	0.667
MOD_GSK3_1	30	37	PF00069	0.512
MOD_GSK3_1	316	323	PF00069	0.535
MOD_GSK3_1	325	332	PF00069	0.553
MOD_GSK3_1	484	491	PF00069	0.472
MOD_GSK3_1	74	81	PF00069	0.758
MOD_GSK3_1	98	105	PF00069	0.481
MOD_N-GLC_1	98	103	PF02516	0.217
MOD_N-GLC_2	30	32	PF02516	0.220
MOD_N-GLC_2	555	557	PF02516	0.228
MOD_NEK2_1	139	144	PF00069	0.501
MOD_NEK2_1	431	436	PF00069	0.429
MOD_NEK2_1	441	446	PF00069	0.416
MOD_OFUCOSY	428	435	PF10250	0.242
MOD_PIKK_1	257	263	PF00454	0.527
MOD_PIKK_1	311	317	PF00454	0.428
MOD_PIKK_1	441	447	PF00454	0.471
MOD_PKA_1	15	21	PF00069	0.581
MOD_PKA_1	153	159	PF00069	0.288
MOD_PKA_1	357	363	PF00069	0.437
MOD_PKA_2	153	159	PF00069	0.393
MOD_PKA_2	357	363	PF00069	0.461
MOD_PKA_2	364	370	PF00069	0.424
MOD_Plk_1	98	104	PF00069	0.417
MOD_Plk_4	249	255	PF00069	0.483
MOD_Plk_4	30	36	PF00069	0.528
MOD_Plk_4	48	54	PF00069	0.427
MOD_Plk_4	98	104	PF00069	0.417
MOD_ProDKin_1	203	209	PF00069	0.754
MOD_ProDKin_1	320	326	PF00069	0.558
MOD_ProDKin_1	62	68	PF00069	0.657
MOD_ProDKin_1	76	82	PF00069	0.699
TRG_DiLeu_BaEn_3	553	559	PF01217	0.425
TRG_DiLeu_BaEn_4	222	228	PF01217	0.561
TRG_DiLeu_BaLyEn_6	437	442	PF01217	0.437
TRG_ENDOCYTIC_2	539	542	PF00928	0.519
TRG_ER_diArg_1	153	155	PF00400	0.318
TRG_ER_diArg_1	356	358	PF00400	0.417
TRG_ER_diArg_1	373	376	PF00400	0.441
TRG_NLS_MonoExtC_3	519	524	PF00514	0.471
TRG_Pf-PMV_PEXEL_1	238	242	PF00026	0.462

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IMP0	Leptomonas seymouri	56%	95%
A0A0S4IU97	Bodo saltans	33%	100%
A0A1X0NKZ4	Trypanosomatidae	34%	97%
A0A3Q8IFC1	Leishmania donovani	90%	90%
A0A3R7N0X9	Trypanosoma rangeli	41%	100%
A4HJ30	Leishmania braziliensis	80%	100%
A4I6K9	Leishmania infantum	91%	90%
A7TSF4	Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294 / BCRC 21397 / CBS 2163 / NBRC 10782 / NRRL Y-8283 / UCD 57-17)	21%	100%
C9ZN61	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	40%	100%
Q12383	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	20%	100%
Q4Q6L1	Leishmania major	89%	100%
V5BII3	Trypanosoma cruzi	40%	100%