Bifunctional lysine-specific demethylase and histidyl-hydroxylase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Cupin superfamily protein, putative

Species:

Leishmania mexicana

UniProt:

E9B1H4_LEIMU

TriTrypDb:

LmxM.30.0240

Length:

624

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	8
GO:0043226	organelle	2	9
GO:0043227	membrane-bounded organelle	3	8
GO:0043229	intracellular organelle	3	9
GO:0043231	intracellular membrane-bounded organelle	4	8
GO:0110165	cellular anatomical entity	1	9
GO:0005730	nucleolus	5	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1

Expansion

Sequence features

E9B1H4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B1H4

Function

Biological processes
Term	Name	Level	Count
GO:0006482	protein demethylation	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	2
GO:0008214	protein dealkylation	5	1
GO:0016570	histone modification	5	1
GO:0016577	histone demethylation	4	1
GO:0019538	protein metabolic process	3	1
GO:0034720	histone H3-K4 demethylation	6	1
GO:0036211	protein modification process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0044238	primary metabolic process	2	1
GO:0070076	histone lysine demethylation	5	1
GO:0070544	histone H3-K36 demethylation	6	1
GO:0070988	demethylation	2	1
GO:0071704	organic substance metabolic process	2	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:0032259	methylation	2	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	9
GO:0005488	binding	1	9
GO:0005506	iron ion binding	6	9
GO:0016491	oxidoreductase activity	2	9
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen	3	9
GO:0016706	2-oxoglutarate-dependent dioxygenase activity	4	9
GO:0043167	ion binding	2	9
GO:0043169	cation binding	3	9
GO:0046872	metal ion binding	4	9
GO:0046914	transition metal ion binding	5	9
GO:0051213	dioxygenase activity	3	9
GO:0032451	demethylase activity	2	1
GO:0032452	histone demethylase activity	4	1
GO:0032453	histone H3K4 demethylase activity	5	1
GO:0051864	histone H3K36 demethylase activity	5	1
GO:0140096	catalytic activity, acting on a protein	2	1
GO:0140457	protein demethylase activity	3	1
GO:0008168	methyltransferase activity	4	1
GO:0016740	transferase activity	2	1
GO:0016741	transferase activity, transferring one-carbon groups	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	605	609	PF00656	0.403
CLV_MEL_PAP_1	240	246	PF00089	0.440
CLV_NRD_NRD_1	105	107	PF00675	0.495
CLV_NRD_NRD_1	112	114	PF00675	0.479
CLV_NRD_NRD_1	179	181	PF00675	0.399
CLV_NRD_NRD_1	185	187	PF00675	0.348
CLV_NRD_NRD_1	242	244	PF00675	0.557
CLV_NRD_NRD_1	381	383	PF00675	0.195
CLV_NRD_NRD_1	422	424	PF00675	0.377
CLV_NRD_NRD_1	494	496	PF00675	0.387
CLV_PCSK_KEX2_1	104	106	PF00082	0.494
CLV_PCSK_KEX2_1	130	132	PF00082	0.647
CLV_PCSK_KEX2_1	185	187	PF00082	0.282
CLV_PCSK_KEX2_1	241	243	PF00082	0.354
CLV_PCSK_KEX2_1	422	424	PF00082	0.407
CLV_PCSK_PC1ET2_1	104	106	PF00082	0.494
CLV_PCSK_PC1ET2_1	130	132	PF00082	0.655
CLV_PCSK_PC1ET2_1	241	243	PF00082	0.354
CLV_PCSK_SKI1_1	422	426	PF00082	0.433
CLV_PCSK_SKI1_1	529	533	PF00082	0.387
DEG_MDM2_SWIB_1	67	74	PF02201	0.424
DEG_Nend_Nbox_1	1	3	PF02207	0.445
DEG_SPOP_SBC_1	509	513	PF00917	0.355
DEG_SPOP_SBC_1	77	81	PF00917	0.422
DOC_CKS1_1	299	304	PF01111	0.259
DOC_CKS1_1	353	358	PF01111	0.324
DOC_CYCLIN_yCln2_LP_2	82	88	PF00134	0.412
DOC_MAPK_gen_1	379	389	PF00069	0.166
DOC_MAPK_HePTP_8	522	534	PF00069	0.411
DOC_MAPK_MEF2A_6	382	391	PF00069	0.308
DOC_MAPK_MEF2A_6	525	534	PF00069	0.402
DOC_MAPK_NFAT4_5	525	533	PF00069	0.387
DOC_PP1_RVXF_1	416	422	PF00149	0.391
DOC_PP2B_LxvP_1	28	31	PF13499	0.494
DOC_PP2B_LxvP_1	368	371	PF13499	0.283
DOC_PP2B_LxvP_1	497	500	PF13499	0.434
DOC_PP2B_LxvP_1	532	535	PF13499	0.451
DOC_PP4_FxxP_1	269	272	PF00568	0.295
DOC_PP4_FxxP_1	353	356	PF00568	0.330
DOC_PP4_FxxP_1	589	592	PF00568	0.328
DOC_USP7_MATH_1	154	158	PF00917	0.621
DOC_USP7_MATH_1	302	306	PF00917	0.259
DOC_USP7_MATH_1	31	35	PF00917	0.499
DOC_USP7_MATH_1	341	345	PF00917	0.280
DOC_USP7_UBL2_3	235	239	PF12436	0.414
DOC_USP7_UBL2_3	379	383	PF12436	0.324
DOC_USP7_UBL2_3	460	464	PF12436	0.278
DOC_WW_Pin1_4	107	112	PF00397	0.544
DOC_WW_Pin1_4	298	303	PF00397	0.259
DOC_WW_Pin1_4	352	357	PF00397	0.324
DOC_WW_Pin1_4	619	624	PF00397	0.455
LIG_14-3-3_CanoR_1	242	247	PF00244	0.449
LIG_14-3-3_CanoR_1	342	350	PF00244	0.270
LIG_14-3-3_CanoR_1	382	388	PF00244	0.247
LIG_14-3-3_CanoR_1	545	550	PF00244	0.348
LIG_APCC_ABBA_1	308	313	PF00400	0.259
LIG_BRCT_BRCA1_1	265	269	PF00533	0.292
LIG_BRCT_BRCA1_1	304	308	PF00533	0.259
LIG_Clathr_ClatBox_1	319	323	PF01394	0.233
LIG_Clathr_ClatBox_1	600	604	PF01394	0.419
LIG_eIF4E_1	246	252	PF01652	0.355
LIG_FHA_1	147	153	PF00498	0.509
LIG_FHA_1	246	252	PF00498	0.455
LIG_FHA_1	442	448	PF00498	0.542
LIG_FHA_1	471	477	PF00498	0.475
LIG_FHA_1	546	552	PF00498	0.361
LIG_FHA_1	576	582	PF00498	0.403
LIG_FHA_1	77	83	PF00498	0.425
LIG_FHA_2	227	233	PF00498	0.228
LIG_FHA_2	603	609	PF00498	0.354
LIG_LIR_Apic_2	266	272	PF02991	0.294
LIG_LIR_Apic_2	298	302	PF02991	0.259
LIG_LIR_Apic_2	351	356	PF02991	0.330
LIG_LIR_Gen_1	398	407	PF02991	0.379
LIG_LIR_Nem_3	177	182	PF02991	0.369
LIG_LIR_Nem_3	287	293	PF02991	0.319
LIG_LIR_Nem_3	325	331	PF02991	0.272
LIG_LIR_Nem_3	398	402	PF02991	0.383
LIG_MYND_1	350	354	PF01753	0.225
LIG_NRBOX	471	477	PF00104	0.352
LIG_PCNA_yPIPBox_3	458	469	PF02747	0.390
LIG_Pex14_1	265	269	PF04695	0.292
LIG_Pex14_2	179	183	PF04695	0.322
LIG_Pex14_2	560	564	PF04695	0.319
LIG_Pex14_2	67	71	PF04695	0.421
LIG_PTB_Apo_2	435	442	PF02174	0.403
LIG_Rb_pABgroove_1	325	333	PF01858	0.324
LIG_REV1ctd_RIR_1	176	185	PF16727	0.344
LIG_SH2_CRK	293	297	PF00017	0.323
LIG_SH2_GRB2like	36	39	PF00017	0.447
LIG_SH2_NCK_1	312	316	PF00017	0.259
LIG_SH2_NCK_1	331	335	PF00017	0.259
LIG_SH2_SRC	312	315	PF00017	0.294
LIG_SH2_SRC	331	334	PF00017	0.294
LIG_SH2_STAT5	182	185	PF00017	0.331
LIG_SH2_STAT5	197	200	PF00017	0.347
LIG_SH2_STAT5	293	296	PF00017	0.379
LIG_SH2_STAT5	36	39	PF00017	0.469
LIG_SH2_STAT5	409	412	PF00017	0.425
LIG_SH2_STAT5	58	61	PF00017	0.438
LIG_SH3_3	202	208	PF00018	0.448
LIG_SH3_3	57	63	PF00018	0.417
LIG_SH3_3	72	78	PF00018	0.443
LIG_SH3_3	97	103	PF00018	0.455
LIG_SUMO_SIM_anti_2	229	235	PF11976	0.338
LIG_SUMO_SIM_anti_2	335	341	PF11976	0.293
LIG_SUMO_SIM_anti_2	471	479	PF11976	0.378
LIG_SUMO_SIM_par_1	471	479	PF11976	0.438
LIG_SUMO_SIM_par_1	599	605	PF11976	0.417
LIG_TYR_ITIM	56	61	PF00017	0.413
LIG_UBA3_1	319	326	PF00899	0.233
MOD_CDC14_SPxK_1	110	113	PF00782	0.483
MOD_CDK_SPK_2	352	357	PF00069	0.280
MOD_CDK_SPxK_1	107	113	PF00069	0.488
MOD_CDK_SPxxK_3	107	114	PF00069	0.509
MOD_CK1_1	157	163	PF00069	0.621
MOD_CK1_1	191	197	PF00069	0.423
MOD_CK1_1	245	251	PF00069	0.427
MOD_CK1_1	298	304	PF00069	0.259
MOD_CK2_1	133	139	PF00069	0.709
MOD_CK2_1	171	177	PF00069	0.329
MOD_GlcNHglycan	126	129	PF01048	0.584
MOD_GlcNHglycan	193	196	PF01048	0.442
MOD_GlcNHglycan	199	202	PF01048	0.414
MOD_GlcNHglycan	279	282	PF01048	0.260
MOD_GlcNHglycan	391	394	PF01048	0.259
MOD_GlcNHglycan	49	52	PF01048	0.413
MOD_GlcNHglycan	504	507	PF01048	0.499
MOD_GlcNHglycan	581	584	PF01048	0.450
MOD_GlcNHglycan	616	619	PF01048	0.664
MOD_GSK3_1	105	112	PF00069	0.586
MOD_GSK3_1	222	229	PF00069	0.455
MOD_GSK3_1	298	305	PF00069	0.259
MOD_GSK3_1	383	390	PF00069	0.243
MOD_GSK3_1	470	477	PF00069	0.377
MOD_GSK3_1	498	505	PF00069	0.470
MOD_GSK3_1	567	574	PF00069	0.377
MOD_GSK3_1	575	582	PF00069	0.413
MOD_GSK3_1	84	91	PF00069	0.453
MOD_N-GLC_1	209	214	PF02516	0.362
MOD_N-GLC_1	37	42	PF02516	0.460
MOD_N-GLC_1	545	550	PF02516	0.409
MOD_NEK2_1	170	175	PF00069	0.310
MOD_NEK2_1	188	193	PF00069	0.326
MOD_NEK2_1	275	280	PF00069	0.519
MOD_NEK2_1	387	392	PF00069	0.259
MOD_NEK2_1	441	446	PF00069	0.483
MOD_NEK2_1	571	576	PF00069	0.401
MOD_NEK2_1	579	584	PF00069	0.433
MOD_NEK2_1	602	607	PF00069	0.372
MOD_NEK2_1	88	93	PF00069	0.432
MOD_NEK2_2	263	268	PF00069	0.272
MOD_NEK2_2	371	376	PF00069	0.334
MOD_NEK2_2	490	495	PF00069	0.477
MOD_PIKK_1	105	111	PF00454	0.512
MOD_PIKK_1	157	163	PF00454	0.621
MOD_PIKK_1	302	308	PF00454	0.259
MOD_PK_1	577	583	PF00069	0.240
MOD_PKA_1	105	111	PF00069	0.540
MOD_PKA_1	124	130	PF00069	0.598
MOD_PKA_1	242	248	PF00069	0.445
MOD_PKA_2	105	111	PF00069	0.490
MOD_PKA_2	242	248	PF00069	0.445
MOD_PKA_2	341	347	PF00069	0.297
MOD_PKA_2	371	377	PF00069	0.293
MOD_PKA_2	441	447	PF00069	0.484
MOD_PKA_2	544	550	PF00069	0.349
MOD_PKB_1	131	139	PF00069	0.583
MOD_PKB_1	519	527	PF00069	0.390
MOD_Plk_1	133	139	PF00069	0.583
MOD_Plk_1	470	476	PF00069	0.374
MOD_Plk_1	545	551	PF00069	0.411
MOD_Plk_4	295	301	PF00069	0.259
MOD_Plk_4	31	37	PF00069	0.480
MOD_Plk_4	470	476	PF00069	0.399
MOD_Plk_4	567	573	PF00069	0.314
MOD_Plk_4	78	84	PF00069	0.412
MOD_Plk_4	88	94	PF00069	0.423
MOD_ProDKin_1	107	113	PF00069	0.545
MOD_ProDKin_1	298	304	PF00069	0.259
MOD_ProDKin_1	352	358	PF00069	0.324
MOD_SUMO_for_1	145	148	PF00179	0.611
MOD_SUMO_rev_2	232	240	PF00179	0.357
TRG_DiLeu_BaEn_1	184	189	PF01217	0.404
TRG_DiLeu_BaEn_1	471	476	PF01217	0.372
TRG_DiLeu_BaEn_1	597	602	PF01217	0.432
TRG_DiLeu_BaLyEn_6	518	523	PF01217	0.452
TRG_DiLeu_BaLyEn_6	526	531	PF01217	0.352
TRG_ENDOCYTIC_2	293	296	PF00928	0.363
TRG_ENDOCYTIC_2	312	315	PF00928	0.249
TRG_ENDOCYTIC_2	58	61	PF00928	0.438
TRG_ER_diArg_1	242	244	PF00400	0.363
TRG_ER_diArg_1	421	423	PF00400	0.382
TRG_ER_diArg_1	519	522	PF00400	0.364
TRG_ER_diArg_1	93	96	PF00400	0.444
TRG_NES_CRM1_1	471	483	PF08389	0.299
TRG_NLS_MonoCore_2	103	108	PF00514	0.489
TRG_NLS_MonoExtN_4	103	108	PF00514	0.489
TRG_NLS_MonoExtN_4	238	245	PF00514	0.449
TRG_Pf-PMV_PEXEL_1	423	428	PF00026	0.419

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PDT4	Leptomonas seymouri	70%	100%
A0A1X0NJI1	Trypanosomatidae	53%	100%
A0A3S7X447	Leishmania donovani	92%	100%
A3KP59	Danio rerio	27%	100%
A4HIZ9	Leishmania braziliensis	78%	100%
A4I6B6	Leishmania infantum	92%	100%
A5PK74	Bos taurus	30%	94%
A8QFQ3	Brugia malayi	26%	88%
A8XEA2	Caenorhabditis briggsae	24%	80%
B0WMG3	Culex quinquefasciatus	30%	96%
B3MSI4	Drosophila ananassae	30%	74%
B3NU20	Drosophila erecta	27%	95%
B4GUZ2	Drosophila persimilis	28%	91%
B4I100	Drosophila sechellia	30%	95%
B4JMQ2	Drosophila grimshawi	29%	86%
B4L6Q5	Drosophila mojavensis	30%	70%
B4M7P8	Drosophila virilis	27%	69%
B4NP88	Drosophila willistoni	30%	81%
B4Q068	Drosophila yakuba	27%	91%
B4R4H1	Drosophila simulans	30%	74%
C3XRY1	Branchiostoma floridae	28%	100%
D3ZU57	Rattus norvegicus	31%	100%
Q16W06	Aedes aegypti	27%	98%
Q4Q6P0	Leishmania major	87%	100%
Q54K96	Dictyostelium discoideum	28%	100%
Q5ZMM1	Gallus gallus	30%	100%
Q7K4H4	Drosophila melanogaster	29%	96%
Q9H6W3	Homo sapiens	30%	97%
Q9JJF3	Mus musculus	31%	100%
V5AYK7	Trypanosoma cruzi	53%	100%