Putative serine/threonine phosphatase

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

serine/threonine phosphatase, putative

Species:

Leishmania mexicana

UniProt:

E9B1A3_LEIMU

TriTrypDb:

LmxM.29.3280

Length:

371

Annotations

LeishMANIAdb annotations

A metalloenzyme with the catalytic domain facing outwards. Related to plant Shewanella-like protein phosphatases. Kinetoplastids have multiple copies of these genes but probably from a very ancient gene duplication.. The cluster might merge two separate, very distantly related groups.

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 7, no: 12
NetGPI	no	yes: 0, no: 19

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	15
GO:0110165	cellular anatomical entity	1	15

Expansion

Sequence features

E9B1A3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B1A3

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	20
GO:0016787	hydrolase activity	2	20

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	152	154	PF00675	0.216
CLV_NRD_NRD_1	234	236	PF00675	0.352
CLV_NRD_NRD_1	31	33	PF00675	0.221
CLV_PCSK_KEX2_1	31	33	PF00082	0.232
CLV_PCSK_SKI1_1	136	140	PF00082	0.228
CLV_PCSK_SKI1_1	154	158	PF00082	0.151
CLV_PCSK_SKI1_1	224	228	PF00082	0.416
CLV_PCSK_SKI1_1	235	239	PF00082	0.452
DEG_Nend_Nbox_1	1	3	PF02207	0.313
DOC_MAPK_gen_1	6	13	PF00069	0.413
DOC_MAPK_MEF2A_6	6	13	PF00069	0.409
DOC_MAPK_NFAT4_5	6	14	PF00069	0.268
DOC_USP7_MATH_1	172	176	PF00917	0.177
DOC_USP7_MATH_1	363	367	PF00917	0.500
DOC_USP7_UBL2_3	120	124	PF12436	0.388
DOC_USP7_UBL2_3	236	240	PF12436	0.227
DOC_WW_Pin1_4	197	202	PF00397	0.212
LIG_Actin_WH2_2	219	237	PF00022	0.153
LIG_Actin_WH2_2	336	354	PF00022	0.144
LIG_APCC_ABBA_1	90	95	PF00400	0.340
LIG_BRCT_BRCA1_1	315	319	PF00533	0.465
LIG_Clathr_ClatBox_1	270	274	PF01394	0.226
LIG_DCNL_PONY_1	1	4	PF03556	0.334
LIG_FHA_1	189	195	PF00498	0.356
LIG_FHA_1	295	301	PF00498	0.258
LIG_FHA_1	51	57	PF00498	0.428
LIG_FHA_2	208	214	PF00498	0.278
LIG_Integrin_RGD_1	116	118	PF01839	0.266
LIG_LIR_Nem_3	118	122	PF02991	0.412
LIG_LIR_Nem_3	239	245	PF02991	0.224
LIG_LIR_Nem_3	365	371	PF02991	0.630
LIG_NRBOX	8	14	PF00104	0.289
LIG_SH2_STAP1	43	47	PF00017	0.490
LIG_SH2_STAT3	347	350	PF00017	0.448
LIG_SH2_STAT5	149	152	PF00017	0.426
LIG_SH2_STAT5	327	330	PF00017	0.234
LIG_SH2_STAT5	348	351	PF00017	0.619
LIG_SH3_3	214	220	PF00018	0.195
LIG_SH3_3	251	257	PF00018	0.266
LIG_SUMO_SIM_anti_2	102	108	PF11976	0.475
LIG_SUMO_SIM_anti_2	332	338	PF11976	0.319
LIG_SUMO_SIM_anti_2	46	51	PF11976	0.411
LIG_SUMO_SIM_anti_2	8	14	PF11976	0.278
LIG_SUMO_SIM_anti_2	81	87	PF11976	0.412
LIG_SUMO_SIM_par_1	219	225	PF11976	0.202
LIG_SUMO_SIM_par_1	269	274	PF11976	0.225
LIG_SUMO_SIM_par_1	32	38	PF11976	0.453
LIG_SUMO_SIM_par_1	327	332	PF11976	0.191
LIG_UBA3_1	1	7	PF00899	0.340
LIG_UBA3_1	176	182	PF00899	0.261
MOD_CDK_SPxxK_3	197	204	PF00069	0.320
MOD_CK1_1	141	147	PF00069	0.235
MOD_CK1_1	170	176	PF00069	0.230
MOD_CK1_1	188	194	PF00069	0.187
MOD_CK1_1	306	312	PF00069	0.474
MOD_CK1_1	313	319	PF00069	0.415
MOD_CK2_1	178	184	PF00069	0.210
MOD_GlcNHglycan	129	132	PF01048	0.315
MOD_GlcNHglycan	13	16	PF01048	0.234
MOD_GlcNHglycan	26	29	PF01048	0.335
MOD_GlcNHglycan	312	315	PF01048	0.640
MOD_GlcNHglycan	331	334	PF01048	0.198
MOD_GlcNHglycan	352	355	PF01048	0.425
MOD_GSK3_1	123	130	PF00069	0.254
MOD_GSK3_1	168	175	PF00069	0.161
MOD_GSK3_1	302	309	PF00069	0.592
MOD_GSK3_1	363	370	PF00069	0.518
MOD_GSK3_1	7	14	PF00069	0.550
MOD_N-GLC_1	141	146	PF02516	0.259
MOD_N-GLC_1	195	200	PF02516	0.286
MOD_N-GLC_1	207	212	PF02516	0.255
MOD_NEK2_1	11	16	PF00069	0.276
MOD_NEK2_1	127	132	PF00069	0.256
MOD_NEK2_1	176	181	PF00069	0.286
MOD_NEK2_1	195	200	PF00069	0.283
MOD_NEK2_1	222	227	PF00069	0.260
MOD_NEK2_1	275	280	PF00069	0.294
MOD_NEK2_1	302	307	PF00069	0.396
MOD_NEK2_1	50	55	PF00069	0.289
MOD_PKA_2	50	56	PF00069	0.305
MOD_Plk_1	294	300	PF00069	0.335
MOD_Plk_1	43	49	PF00069	0.282
MOD_Plk_4	172	178	PF00069	0.158
MOD_Plk_4	244	250	PF00069	0.210
MOD_Plk_4	307	313	PF00069	0.698
MOD_Plk_4	45	51	PF00069	0.243
MOD_Plk_4	7	13	PF00069	0.447
MOD_ProDKin_1	197	203	PF00069	0.245
MOD_SUMO_rev_2	316	325	PF00179	0.385
TRG_DiLeu_BaEn_1	65	70	PF01217	0.380
TRG_DiLeu_BaLyEn_6	266	271	PF01217	0.292
TRG_DiLeu_BaLyEn_6	356	361	PF01217	0.442
TRG_DiLeu_BaLyEn_6	51	56	PF01217	0.405
TRG_DiLeu_BaLyEn_6	84	89	PF01217	0.335
TRG_ENDOCYTIC_2	119	122	PF00928	0.294
TRG_ENDOCYTIC_2	159	162	PF00928	0.391
TRG_ENDOCYTIC_2	326	329	PF00928	0.210
TRG_ENDOCYTIC_2	348	351	PF00928	0.359
TRG_ENDOCYTIC_2	368	371	PF00928	0.477
TRG_ER_diArg_1	150	153	PF00400	0.230
TRG_ER_diArg_1	31	33	PF00400	0.230
TRG_NES_CRM1_1	66	82	PF08389	0.333

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I3V2	Leptomonas seymouri	75%	99%
A0A0N1P9R1	Leptomonas seymouri	26%	100%
A0A0S4JBT9	Bodo saltans	33%	95%
A0A0S4JMN0	Bodo saltans	28%	92%
A0A0S4KJG1	Bodo saltans	51%	100%
A0A1X0NU01	Trypanosomatidae	24%	99%
A0A1X0NZX7	Trypanosomatidae	29%	83%
A0A1X0P2G6	Trypanosomatidae	65%	100%
A0A3Q8IBB4	Leishmania donovani	29%	100%
A0A3Q8IIK0	Leishmania donovani	30%	98%
A0A3R7NTC0	Trypanosoma rangeli	64%	100%
A0A3S5IRW3	Trypanosoma rangeli	27%	90%
A0A3S7X3U9	Leishmania donovani	93%	100%
A4HCJ2	Leishmania braziliensis	26%	100%
A4HH45	Leishmania braziliensis	28%	100%
A4HIR7	Leishmania braziliensis	81%	100%
A4I008	Leishmania infantum	29%	100%
A4I498	Leishmania infantum	29%	98%
A4I612	Leishmania infantum	92%	100%
C9ZQ86	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	27%	99%
C9ZRD7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	62%	84%
E9ADP5	Leishmania major	31%	100%
E9AM35	Leishmania mexicana (strain MHOM/GT/2001/U1103)	30%	100%
E9AVY1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	100%
O74480	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	29%	100%
Q4Q6W1	Leishmania major	93%	100%
Q4QBJ8	Leishmania major	27%	100%
Q944L7	Arabidopsis thaliana	26%	95%
V5ARZ9	Trypanosoma cruzi	25%	99%
V5BPY7	Trypanosoma cruzi	28%	85%
V5BX32	Trypanosoma cruzi	63%	100%