SAM-dependent MTase TRM10-type domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tRNA (Guanine-1)-methyltransferase, putative

Species:

Leishmania mexicana

UniProt:

E9B181_LEIMU

TriTrypDb:

LmxM.29.3070

Length:

387

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9B181

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B181

Function

Biological processes
Term	Name	Level	Count
GO:0008152	metabolic process	1	8
GO:0032259	methylation	2	8
GO:0001510	RNA methylation	4	1
GO:0002939	tRNA N1-guanine methylation	6	1
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006396	RNA processing	6	1
GO:0006399	tRNA metabolic process	7	1
GO:0006400	tRNA modification	6	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008033	tRNA processing	8	1
GO:0009451	RNA modification	5	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0030488	tRNA methylation	5	1
GO:0034470	ncRNA processing	7	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034660	ncRNA metabolic process	6	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0043414	macromolecule methylation	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	10
GO:0008168	methyltransferase activity	4	10
GO:0016740	transferase activity	2	10
GO:0016741	transferase activity, transferring one-carbon groups	3	10
GO:0000049	tRNA binding	5	1
GO:0003676	nucleic acid binding	3	1
GO:0003723	RNA binding	4	1
GO:0005488	binding	1	1
GO:0008173	RNA methyltransferase activity	4	3
GO:0008175	tRNA methyltransferase activity	5	3
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	3
GO:0009019	tRNA (guanine-N1-)-methyltransferase activity	7	3
GO:0016423	tRNA (guanine) methyltransferase activity	6	3
GO:0097159	organic cyclic compound binding	2	1
GO:0140098	catalytic activity, acting on RNA	3	3
GO:0140101	catalytic activity, acting on a tRNA	4	3
GO:0140640	catalytic activity, acting on a nucleic acid	2	3
GO:1901363	heterocyclic compound binding	2	1
GO:0052905	tRNA (guanine(9)-N(1))-methyltransferase activity	8	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	185	189	PF00656	0.389
CLV_C14_Caspase3-7	24	28	PF00656	0.523
CLV_C14_Caspase3-7	35	39	PF00656	0.671
CLV_C14_Caspase3-7	370	374	PF00656	0.681
CLV_NRD_NRD_1	104	106	PF00675	0.498
CLV_NRD_NRD_1	115	117	PF00675	0.397
CLV_NRD_NRD_1	210	212	PF00675	0.227
CLV_NRD_NRD_1	329	331	PF00675	0.506
CLV_NRD_NRD_1	338	340	PF00675	0.610
CLV_NRD_NRD_1	55	57	PF00675	0.569
CLV_NRD_NRD_1	77	79	PF00675	0.436
CLV_PCSK_FUR_1	338	342	PF00082	0.578
CLV_PCSK_FUR_1	53	57	PF00082	0.550
CLV_PCSK_FUR_1	75	79	PF00082	0.440
CLV_PCSK_KEX2_1	104	106	PF00082	0.498
CLV_PCSK_KEX2_1	114	116	PF00082	0.403
CLV_PCSK_KEX2_1	210	212	PF00082	0.227
CLV_PCSK_KEX2_1	329	331	PF00082	0.506
CLV_PCSK_KEX2_1	337	339	PF00082	0.426
CLV_PCSK_KEX2_1	340	342	PF00082	0.560
CLV_PCSK_KEX2_1	55	57	PF00082	0.528
CLV_PCSK_KEX2_1	63	65	PF00082	0.410
CLV_PCSK_KEX2_1	74	76	PF00082	0.342
CLV_PCSK_KEX2_1	77	79	PF00082	0.387
CLV_PCSK_PC1ET2_1	340	342	PF00082	0.567
CLV_PCSK_PC1ET2_1	63	65	PF00082	0.466
CLV_PCSK_PC1ET2_1	74	76	PF00082	0.506
CLV_PCSK_SKI1_1	293	297	PF00082	0.213
CLV_PCSK_SKI1_1	63	67	PF00082	0.470
DOC_MAPK_gen_1	101	111	PF00069	0.432
DOC_MAPK_gen_1	337	346	PF00069	0.600
DOC_PP2B_LxvP_1	225	228	PF13499	0.428
DOC_USP7_MATH_1	16	20	PF00917	0.642
DOC_USP7_MATH_1	31	35	PF00917	0.510
DOC_USP7_MATH_1	321	325	PF00917	0.454
DOC_USP7_MATH_1	372	376	PF00917	0.730
DOC_USP7_MATH_1	41	45	PF00917	0.577
DOC_USP7_UBL2_3	70	74	PF12436	0.443
DOC_WW_Pin1_4	235	240	PF00397	0.428
DOC_WW_Pin1_4	312	317	PF00397	0.508
DOC_WW_Pin1_4	9	14	PF00397	0.604
LIG_14-3-3_CanoR_1	215	222	PF00244	0.342
LIG_14-3-3_CanoR_1	329	335	PF00244	0.403
LIG_14-3-3_CanoR_1	341	347	PF00244	0.685
LIG_14-3-3_CanoR_1	53	59	PF00244	0.527
LIG_Actin_WH2_2	153	170	PF00022	0.508
LIG_Actin_WH2_2	296	313	PF00022	0.508
LIG_BIR_III_4	192	196	PF00653	0.342
LIG_BRCT_BRCA1_1	222	226	PF00533	0.508
LIG_CaM_NSCaTE_8	318	325	PF13499	0.428
LIG_EH1_1	298	306	PF00400	0.508
LIG_FHA_1	21	27	PF00498	0.508
LIG_FHA_1	247	253	PF00498	0.448
LIG_FHA_1	275	281	PF00498	0.469
LIG_FHA_1	329	335	PF00498	0.592
LIG_FHA_2	122	128	PF00498	0.247
LIG_FHA_2	241	247	PF00498	0.474
LIG_FHA_2	345	351	PF00498	0.470
LIG_FHA_2	57	63	PF00498	0.449
LIG_FHA_2	95	101	PF00498	0.442
LIG_LIR_Gen_1	163	172	PF02991	0.427
LIG_LIR_Gen_1	92	100	PF02991	0.317
LIG_LIR_Nem_3	163	167	PF02991	0.427
LIG_LIR_Nem_3	207	212	PF02991	0.421
LIG_LIR_Nem_3	92	96	PF02991	0.317
LIG_Pex14_1	222	226	PF04695	0.427
LIG_Pex14_2	140	144	PF04695	0.265
LIG_RPA_C_Fungi	110	122	PF08784	0.434
LIG_SH2_CRK	164	168	PF00017	0.296
LIG_SH2_NCK_1	267	271	PF00017	0.381
LIG_SH2_STAT5	251	254	PF00017	0.273
LIG_SH3_3	10	16	PF00018	0.621
LIG_SH3_3	170	176	PF00018	0.267
LIG_TRAF2_1	377	380	PF00917	0.490
LIG_TRAF2_1	59	62	PF00917	0.414
LIG_TRAF2_1	97	100	PF00917	0.434
LIG_TYR_ITIM	162	167	PF00017	0.269
LIG_TYR_ITIM	305	310	PF00017	0.381
LIG_WW_3	212	216	PF00397	0.149
MOD_CK1_1	155	161	PF00069	0.248
MOD_CK1_1	20	26	PF00069	0.591
MOD_CK1_1	238	244	PF00069	0.269
MOD_CK1_1	266	272	PF00069	0.313
MOD_CK1_1	44	50	PF00069	0.609
MOD_CK2_1	121	127	PF00069	0.560
MOD_CK2_1	184	190	PF00069	0.381
MOD_CK2_1	240	246	PF00069	0.273
MOD_CK2_1	362	368	PF00069	0.701
MOD_CK2_1	54	60	PF00069	0.500
MOD_CK2_1	94	100	PF00069	0.491
MOD_GlcNHglycan	164	167	PF01048	0.297
MOD_GlcNHglycan	23	26	PF01048	0.590
MOD_GlcNHglycan	268	271	PF01048	0.417
MOD_GlcNHglycan	323	326	PF01048	0.354
MOD_GlcNHglycan	373	377	PF01048	0.655
MOD_GlcNHglycan	4	7	PF01048	0.460
MOD_GSK3_1	146	153	PF00069	0.445
MOD_GSK3_1	16	23	PF00069	0.616
MOD_GSK3_1	162	169	PF00069	0.368
MOD_GSK3_1	321	328	PF00069	0.392
MOD_GSK3_1	342	349	PF00069	0.707
MOD_GSK3_1	362	369	PF00069	0.702
MOD_GSK3_1	41	48	PF00069	0.463
MOD_N-GLC_1	31	36	PF02516	0.527
MOD_N-GLC_2	290	292	PF02516	0.248
MOD_NEK2_1	130	135	PF00069	0.307
MOD_NEK2_1	140	145	PF00069	0.294
MOD_NEK2_1	162	167	PF00069	0.261
MOD_NEK2_1	2	7	PF00069	0.501
MOD_NEK2_1	310	315	PF00069	0.221
MOD_NEK2_1	344	349	PF00069	0.466
MOD_PIKK_1	155	161	PF00454	0.248
MOD_PIKK_1	214	220	PF00454	0.313
MOD_PIKK_1	226	232	PF00454	0.269
MOD_PIKK_1	328	334	PF00454	0.409
MOD_PKA_2	21	27	PF00069	0.532
MOD_PKA_2	214	220	PF00069	0.149
MOD_PKA_2	310	316	PF00069	0.355
MOD_PKA_2	328	334	PF00069	0.461
MOD_PKA_2	54	60	PF00069	0.561
MOD_Plk_1	140	146	PF00069	0.300
MOD_Plk_2-3	357	363	PF00069	0.737
MOD_Plk_4	177	183	PF00069	0.367
MOD_Plk_4	282	288	PF00069	0.391
MOD_ProDKin_1	235	241	PF00069	0.269
MOD_ProDKin_1	312	318	PF00069	0.381
MOD_ProDKin_1	9	15	PF00069	0.605
MOD_SUMO_rev_2	57	65	PF00179	0.461
MOD_SUMO_rev_2	98	103	PF00179	0.448
TRG_ENDOCYTIC_2	164	167	PF00928	0.296
TRG_ENDOCYTIC_2	206	209	PF00928	0.361
TRG_ENDOCYTIC_2	307	310	PF00928	0.384
TRG_ER_diArg_1	114	116	PF00400	0.400
TRG_ER_diArg_1	209	211	PF00400	0.267
TRG_ER_diArg_1	337	339	PF00400	0.644
TRG_ER_diArg_1	53	56	PF00400	0.637
TRG_NLS_Bipartite_1	329	343	PF00514	0.649
TRG_NLS_MonoExtN_4	337	343	PF00514	0.505

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I770	Leptomonas seymouri	68%	100%
A0A0S4JPX3	Bodo saltans	42%	100%
A0A1X0P1F8	Trypanosomatidae	47%	100%
A0A3S5ISH7	Trypanosoma rangeli	52%	100%
A0A3S7X3S5	Leishmania donovani	87%	100%
A4HIP1	Leishmania braziliensis	75%	100%
A4I5Y8	Leishmania infantum	86%	100%
Q08DP1	Bos taurus	26%	100%
Q4I8X0	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	28%	95%
Q4KLI2	Rattus norvegicus	23%	100%
Q4Q6Y3	Leishmania major	88%	100%
Q4WXA1	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	27%	98%
Q59Q39	Candida albicans (strain SC5314 / ATCC MYA-2876)	25%	100%
Q5B8X0	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	27%	93%
Q5RJK3	Rattus norvegicus	25%	100%
Q6BWG3	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	23%	100%
Q6CUM6	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	30%	100%
Q6FQB2	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	29%	100%
Q6PF06	Homo sapiens	25%	100%
Q7L0Y3	Homo sapiens	26%	96%
Q9D075	Mus musculus	24%	100%
V5DNZ8	Trypanosoma cruzi	47%	100%