GRAM domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

GRAM domain containing protein, putative

Species:

Leishmania mexicana

UniProt:

E9B144_LEIMU

TriTrypDb:

LmxM.29.2720

Length:

730

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 7
NetGPI	no	yes: 0, no: 7

Cellular components
Term	Name	Level	Count
GO:0005789	endoplasmic reticulum membrane	4	1
GO:0005886	plasma membrane	3	1
GO:0016020	membrane	2	1
GO:0031090	organelle membrane	3	1
GO:0044232	organelle membrane contact site	2	1
GO:0110165	cellular anatomical entity	1	1
GO:0140268	endoplasmic reticulum-plasma membrane contact site	3	1

Expansion

Sequence features

E9B144

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B144

PDB structure(s): 4tyz_A , 4tyz_B

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	1
GO:0006869	lipid transport	5	1
GO:0009987	cellular process	1	1
GO:0015850	organic hydroxy compound transport	5	1
GO:0015918	sterol transport	6	1
GO:0032365	intracellular lipid transport	4	1
GO:0032366	intracellular sterol transport	5	1
GO:0046907	intracellular transport	3	1
GO:0051179	localization	1	1
GO:0051234	establishment of localization	2	1
GO:0051641	cellular localization	2	1
GO:0051649	establishment of localization in cell	3	1
GO:0071702	organic substance transport	4	1

Molecular functions
Term	Name	Level	Count
GO:0005215	transporter activity	1	1
GO:0005319	lipid transporter activity	2	1
GO:0005488	binding	1	1
GO:0005496	steroid binding	3	1
GO:0008289	lipid binding	2	1
GO:0015248	sterol transporter activity	3	1
GO:0032934	sterol binding	4	1
GO:0097159	organic cyclic compound binding	2	1
GO:0120013	lipid transfer activity	3	1
GO:0120015	sterol transfer activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	46	50	PF00656	0.397
CLV_C14_Caspase3-7	473	477	PF00656	0.519
CLV_C14_Caspase3-7	520	524	PF00656	0.551
CLV_C14_Caspase3-7	637	641	PF00656	0.429
CLV_NRD_NRD_1	151	153	PF00675	0.520
CLV_NRD_NRD_1	186	188	PF00675	0.815
CLV_NRD_NRD_1	434	436	PF00675	0.496
CLV_NRD_NRD_1	626	628	PF00675	0.375
CLV_PCSK_KEX2_1	151	153	PF00082	0.632
CLV_PCSK_KEX2_1	185	187	PF00082	0.823
CLV_PCSK_KEX2_1	434	436	PF00082	0.451
CLV_PCSK_SKI1_1	134	138	PF00082	0.558
CLV_PCSK_SKI1_1	292	296	PF00082	0.748
CLV_PCSK_SKI1_1	544	548	PF00082	0.748
CLV_PCSK_SKI1_1	649	653	PF00082	0.373
CLV_PCSK_SKI1_1	689	693	PF00082	0.371
DEG_Nend_UBRbox_1	1	4	PF02207	0.493
DEG_SPOP_SBC_1	143	147	PF00917	0.586
DOC_CKS1_1	658	663	PF01111	0.374
DOC_CYCLIN_yCln2_LP_2	594	600	PF00134	0.660
DOC_MAPK_DCC_7	314	322	PF00069	0.420
DOC_MAPK_MEF2A_6	314	322	PF00069	0.420
DOC_MAPK_MEF2A_6	483	492	PF00069	0.304
DOC_PP1_RVXF_1	542	549	PF00149	0.696
DOC_PP2B_LxvP_1	594	597	PF13499	0.664
DOC_PP4_FxxP_1	401	404	PF00568	0.457
DOC_PP4_FxxP_1	559	562	PF00568	0.718
DOC_PP4_FxxP_1	658	661	PF00568	0.358
DOC_SPAK_OSR1_1	63	67	PF12202	0.311
DOC_USP7_MATH_1	144	148	PF00917	0.625
DOC_USP7_MATH_1	153	157	PF00917	0.711
DOC_USP7_MATH_1	176	180	PF00917	0.807
DOC_USP7_MATH_1	232	236	PF00917	0.764
DOC_USP7_MATH_1	273	277	PF00917	0.713
DOC_USP7_MATH_1	34	38	PF00917	0.589
DOC_USP7_MATH_1	528	532	PF00917	0.623
DOC_USP7_MATH_1	722	726	PF00917	0.500
DOC_USP7_UBL2_3	220	224	PF12436	0.699
DOC_USP7_UBL2_3	683	687	PF12436	0.429
DOC_WW_Pin1_4	160	165	PF00397	0.682
DOC_WW_Pin1_4	214	219	PF00397	0.552
DOC_WW_Pin1_4	238	243	PF00397	0.714
DOC_WW_Pin1_4	253	258	PF00397	0.748
DOC_WW_Pin1_4	302	307	PF00397	0.647
DOC_WW_Pin1_4	4	9	PF00397	0.591
DOC_WW_Pin1_4	400	405	PF00397	0.454
DOC_WW_Pin1_4	657	662	PF00397	0.374
LIG_14-3-3_CanoR_1	27	36	PF00244	0.638
LIG_14-3-3_CanoR_1	434	440	PF00244	0.456
LIG_14-3-3_CanoR_1	483	490	PF00244	0.565
LIG_14-3-3_CanoR_1	627	631	PF00244	0.377
LIG_14-3-3_CanoR_1	63	67	PF00244	0.414
LIG_AP2alpha_2	399	401	PF02296	0.530
LIG_BIR_III_4	610	614	PF00653	0.575
LIG_deltaCOP1_diTrp_1	120	130	PF00928	0.386
LIG_deltaCOP1_diTrp_1	206	213	PF00928	0.540
LIG_eIF4E_1	437	443	PF01652	0.424
LIG_FHA_1	24	30	PF00498	0.523
LIG_FHA_1	321	327	PF00498	0.486
LIG_FHA_1	354	360	PF00498	0.434
LIG_FHA_1	450	456	PF00498	0.494
LIG_FHA_1	483	489	PF00498	0.486
LIG_FHA_1	49	55	PF00498	0.654
LIG_FHA_1	492	498	PF00498	0.569
LIG_FHA_1	514	520	PF00498	0.604
LIG_FHA_1	56	62	PF00498	0.499
LIG_FHA_1	705	711	PF00498	0.384
LIG_FHA_2	303	309	PF00498	0.609
LIG_FHA_2	347	353	PF00498	0.515
LIG_FHA_2	471	477	PF00498	0.489
LIG_FHA_2	5	11	PF00498	0.598
LIG_FHA_2	635	641	PF00498	0.429
LIG_LIR_Apic_2	347	353	PF02991	0.580
LIG_LIR_Apic_2	399	404	PF02991	0.469
LIG_LIR_Apic_2	556	562	PF02991	0.718
LIG_LIR_Gen_1	316	326	PF02991	0.480
LIG_LIR_Gen_1	355	364	PF02991	0.428
LIG_LIR_Gen_1	438	447	PF02991	0.459
LIG_LIR_Gen_1	480	488	PF02991	0.552
LIG_LIR_Gen_1	516	526	PF02991	0.537
LIG_LIR_Gen_1	660	671	PF02991	0.387
LIG_LIR_Gen_1	676	686	PF02991	0.245
LIG_LIR_Gen_1	717	728	PF02991	0.376
LIG_LIR_Nem_3	316	322	PF02991	0.473
LIG_LIR_Nem_3	355	361	PF02991	0.426
LIG_LIR_Nem_3	366	372	PF02991	0.391
LIG_LIR_Nem_3	438	443	PF02991	0.422
LIG_LIR_Nem_3	480	484	PF02991	0.551
LIG_LIR_Nem_3	516	521	PF02991	0.530
LIG_LIR_Nem_3	578	582	PF02991	0.525
LIG_LIR_Nem_3	629	633	PF02991	0.358
LIG_LIR_Nem_3	660	666	PF02991	0.387
LIG_LIR_Nem_3	676	682	PF02991	0.277
LIG_LIR_Nem_3	717	723	PF02991	0.429
LIG_PCNA_yPIPBox_3	351	363	PF02747	0.570
LIG_PCNA_yPIPBox_3	716	728	PF02747	0.212
LIG_Pex14_2	104	108	PF04695	0.521
LIG_Pex14_2	626	630	PF04695	0.376
LIG_Pex14_2	642	646	PF04695	0.376
LIG_Pex14_2	709	713	PF04695	0.376
LIG_REV1ctd_RIR_1	135	143	PF16727	0.649
LIG_SH2_CRK	440	444	PF00017	0.391
LIG_SH2_PTP2	358	361	PF00017	0.415
LIG_SH2_SRC	679	682	PF00017	0.429
LIG_SH2_STAP1	208	212	PF00017	0.723
LIG_SH2_STAP1	437	441	PF00017	0.428
LIG_SH2_STAT5	319	322	PF00017	0.471
LIG_SH2_STAT5	358	361	PF00017	0.415
LIG_SH2_STAT5	369	372	PF00017	0.399
LIG_SH2_STAT5	375	378	PF00017	0.447
LIG_SH2_STAT5	437	440	PF00017	0.430
LIG_SH2_STAT5	656	659	PF00017	0.429
LIG_SH3_2	283	288	PF14604	0.822
LIG_SH3_3	251	257	PF00018	0.542
LIG_SH3_3	280	286	PF00018	0.770
LIG_SH3_3	516	522	PF00018	0.493
LIG_SH3_3	543	549	PF00018	0.716
LIG_SUMO_SIM_anti_2	30	37	PF11976	0.566
LIG_SUMO_SIM_par_1	470	476	PF11976	0.497
LIG_TRAF2_2	549	554	PF00917	0.588
LIG_TYR_ITIM	677	682	PF00017	0.429
LIG_TYR_ITSM	436	443	PF00017	0.477
LIG_WRC_WIRS_1	105	110	PF05994	0.532
LIG_WRC_WIRS_1	345	350	PF05994	0.522
LIG_WRC_WIRS_1	505	510	PF05994	0.513
LIG_WRC_WIRS_1	639	644	PF05994	0.429
LIG_WW_3	445	449	PF00397	0.514
MOD_CDC14_SPxK_1	217	220	PF00782	0.522
MOD_CDK_SPK_2	214	219	PF00069	0.523
MOD_CDK_SPxK_1	214	220	PF00069	0.526
MOD_CDK_SPxxK_3	238	245	PF00069	0.661
MOD_CDK_SPxxK_3	253	260	PF00069	0.545
MOD_CK1_1	142	148	PF00069	0.662
MOD_CK1_1	23	29	PF00069	0.503
MOD_CK1_1	256	262	PF00069	0.763
MOD_CK1_1	290	296	PF00069	0.695
MOD_CK1_1	62	68	PF00069	0.511
MOD_CK2_1	321	327	PF00069	0.482
MOD_CK2_1	346	352	PF00069	0.519
MOD_CK2_1	4	10	PF00069	0.610
MOD_CK2_1	521	527	PF00069	0.485
MOD_CK2_1	78	84	PF00069	0.504
MOD_Cter_Amidation	183	186	PF01082	0.784
MOD_GlcNHglycan	210	213	PF01048	0.841
MOD_GlcNHglycan	22	25	PF01048	0.544
MOD_GlcNHglycan	233	237	PF01048	0.741
MOD_GlcNHglycan	261	264	PF01048	0.738
MOD_GlcNHglycan	275	278	PF01048	0.721
MOD_GlcNHglycan	378	381	PF01048	0.596
MOD_GlcNHglycan	510	513	PF01048	0.580
MOD_GlcNHglycan	648	652	PF01048	0.438
MOD_GSK3_1	103	110	PF00069	0.516
MOD_GSK3_1	138	145	PF00069	0.602
MOD_GSK3_1	147	154	PF00069	0.636
MOD_GSK3_1	170	177	PF00069	0.756
MOD_GSK3_1	23	30	PF00069	0.488
MOD_GSK3_1	234	241	PF00069	0.768
MOD_GSK3_1	255	262	PF00069	0.782
MOD_GSK3_1	273	280	PF00069	0.608
MOD_GSK3_1	340	347	PF00069	0.473
MOD_GSK3_1	43	50	PF00069	0.451
MOD_GSK3_1	504	511	PF00069	0.509
MOD_GSK3_1	55	62	PF00069	0.486
MOD_GSK3_1	634	641	PF00069	0.429
MOD_GSK3_1	665	672	PF00069	0.228
MOD_GSK3_1	67	74	PF00069	0.543
MOD_GSK3_1	704	711	PF00069	0.350
MOD_LATS_1	25	31	PF00433	0.560
MOD_N-GLC_1	20	25	PF02516	0.527
MOD_N-GLC_1	288	293	PF02516	0.809
MOD_N-GLC_1	70	75	PF02516	0.465
MOD_N-GLC_1	704	709	PF02516	0.413
MOD_NEK2_1	104	109	PF00069	0.523
MOD_NEK2_1	138	143	PF00069	0.591
MOD_NEK2_1	320	325	PF00069	0.521
MOD_NEK2_1	506	511	PF00069	0.517
MOD_NEK2_1	513	518	PF00069	0.522
MOD_NEK2_1	553	558	PF00069	0.719
MOD_NEK2_1	609	614	PF00069	0.571
MOD_NEK2_1	626	631	PF00069	0.283
MOD_NEK2_1	638	643	PF00069	0.311
MOD_NEK2_1	666	671	PF00069	0.459
MOD_NEK2_1	704	709	PF00069	0.366
MOD_OFUCOSY	69	75	PF10250	0.585
MOD_PIKK_1	107	113	PF00454	0.522
MOD_PIKK_1	34	40	PF00454	0.593
MOD_PIKK_1	496	502	PF00454	0.569
MOD_PIKK_1	722	728	PF00454	0.459
MOD_PK_1	139	145	PF00069	0.615
MOD_PK_1	687	693	PF00069	0.500
MOD_PKA_1	151	157	PF00069	0.523
MOD_PKA_1	224	230	PF00069	0.805
MOD_PKA_2	138	144	PF00069	0.544
MOD_PKA_2	147	153	PF00069	0.657
MOD_PKA_2	259	265	PF00069	0.671
MOD_PKA_2	482	488	PF00069	0.561
MOD_PKA_2	62	68	PF00069	0.406
MOD_PKA_2	626	632	PF00069	0.377
MOD_Plk_1	11	17	PF00069	0.329
MOD_Plk_1	139	145	PF00069	0.615
MOD_Plk_1	263	269	PF00069	0.724
MOD_Plk_1	414	420	PF00069	0.572
MOD_Plk_1	59	65	PF00069	0.595
MOD_Plk_1	647	653	PF00069	0.377
MOD_Plk_1	704	710	PF00069	0.413
MOD_Plk_2-3	78	84	PF00069	0.430
MOD_Plk_4	104	110	PF00069	0.472
MOD_Plk_4	353	359	PF00069	0.478
MOD_Plk_4	435	441	PF00069	0.431
MOD_Plk_4	470	476	PF00069	0.554
MOD_Plk_4	634	640	PF00069	0.380
MOD_Plk_4	704	710	PF00069	0.376
MOD_ProDKin_1	160	166	PF00069	0.683
MOD_ProDKin_1	214	220	PF00069	0.555
MOD_ProDKin_1	238	244	PF00069	0.714
MOD_ProDKin_1	253	259	PF00069	0.749
MOD_ProDKin_1	302	308	PF00069	0.642
MOD_ProDKin_1	4	10	PF00069	0.586
MOD_ProDKin_1	400	406	PF00069	0.452
MOD_ProDKin_1	657	663	PF00069	0.374
MOD_SUMO_rev_2	676	685	PF00179	0.429
MOD_SUMO_rev_2	695	702	PF00179	0.415
TRG_DiLeu_BaEn_1	316	321	PF01217	0.477
TRG_DiLeu_BaLyEn_6	96	101	PF01217	0.548
TRG_ENDOCYTIC_2	116	119	PF00928	0.485
TRG_ENDOCYTIC_2	319	322	PF00928	0.471
TRG_ENDOCYTIC_2	358	361	PF00928	0.415
TRG_ENDOCYTIC_2	440	443	PF00928	0.418
TRG_ENDOCYTIC_2	656	659	PF00928	0.429
TRG_ENDOCYTIC_2	679	682	PF00928	0.376
TRG_ER_diArg_1	151	153	PF00400	0.520
TRG_ER_diArg_1	185	187	PF00400	0.720
TRG_ER_diArg_1	434	436	PF00400	0.496
TRG_Pf-PMV_PEXEL_1	27	31	PF00026	0.561

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I443	Leptomonas seymouri	57%	100%
A0A3R7L2R2	Trypanosoma rangeli	35%	100%
A0A3S7X3S1	Leishmania donovani	89%	100%
A4HIK4	Leishmania braziliensis	70%	97%
A4I5U9	Leishmania infantum	89%	100%
Q4Q722	Leishmania major	87%	100%