RING-type domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Ring-between-ring E3 ligase, putative

Species:

Leishmania mexicana

UniProt:

E9B0Z0_LEIMU

TriTrypDb:

LmxM.29.2170

Length:

518

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0000151	ubiquitin ligase complex	3	1
GO:0005737	cytoplasm	2	1
GO:0032991	protein-containing complex	1	1
GO:0110165	cellular anatomical entity	1	1
GO:0140535	intracellular protein-containing complex	2	1
GO:1902494	catalytic complex	2	1
GO:1990234	transferase complex	3	1

Expansion

Sequence features

E9B0Z0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B0Z0

Function

Biological processes
Term	Name	Level	Count
GO:0000209	protein polyubiquitination	8	1
GO:0006508	proteolysis	4	1
GO:0006511	ubiquitin-dependent protein catabolic process	7	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009056	catabolic process	2	1
GO:0009057	macromolecule catabolic process	4	1
GO:0009893	positive regulation of metabolic process	4	1
GO:0009894	regulation of catabolic process	4	1
GO:0009896	positive regulation of catabolic process	5	1
GO:0009987	cellular process	1	1
GO:0010604	positive regulation of macromolecule metabolic process	5	1
GO:0016567	protein ubiquitination	7	1
GO:0019222	regulation of metabolic process	3	1
GO:0019538	protein metabolic process	3	1
GO:0019941	modification-dependent protein catabolic process	6	1
GO:0030162	regulation of proteolysis	6	1
GO:0031323	regulation of cellular metabolic process	4	1
GO:0031325	positive regulation of cellular metabolic process	5	1
GO:0031329	regulation of cellular catabolic process	5	1
GO:0031331	positive regulation of cellular catabolic process	6	1
GO:0032434	regulation of proteasomal ubiquitin-dependent protein catabolic process	7	1
GO:0032436	positive regulation of proteasomal ubiquitin-dependent protein catabolic process	8	1
GO:0032446	protein modification by small protein conjugation	6	1
GO:0036211	protein modification process	4	1
GO:0042176	regulation of protein catabolic process	5	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0043632	modification-dependent macromolecule catabolic process	5	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044248	cellular catabolic process	3	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0044265	obsolete cellular macromolecule catabolic process	4	1
GO:0045732	positive regulation of protein catabolic process	6	1
GO:0045862	positive regulation of proteolysis	7	1
GO:0048518	positive regulation of biological process	3	1
GO:0048522	positive regulation of cellular process	4	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0051171	regulation of nitrogen compound metabolic process	4	1
GO:0051173	positive regulation of nitrogen compound metabolic process	5	1
GO:0051246	regulation of protein metabolic process	5	1
GO:0051247	positive regulation of protein metabolic process	6	1
GO:0051603	proteolysis involved in protein catabolic process	5	1
GO:0060255	regulation of macromolecule metabolic process	4	1
GO:0061136	regulation of proteasomal protein catabolic process	6	1
GO:0065007	biological regulation	1	1
GO:0070647	protein modification by small protein conjugation or removal	5	1
GO:0071704	organic substance metabolic process	2	1
GO:0080090	regulation of primary metabolic process	4	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1901575	organic substance catabolic process	3	1
GO:1901800	positive regulation of proteasomal protein catabolic process	7	1
GO:1903050	regulation of proteolysis involved in protein catabolic process	7	1
GO:1903052	positive regulation of proteolysis involved in protein catabolic process	8	1
GO:2000058	regulation of ubiquitin-dependent protein catabolic process	6	1
GO:2000060	positive regulation of ubiquitin-dependent protein catabolic process	7	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	10
GO:0004842	ubiquitin-protein transferase activity	4	10
GO:0005488	binding	1	9
GO:0016740	transferase activity	2	10
GO:0019787	ubiquitin-like protein transferase activity	3	10
GO:0043167	ion binding	2	9
GO:0043169	cation binding	3	9
GO:0046872	metal ion binding	4	9
GO:0140096	catalytic activity, acting on a protein	2	10
GO:0005515	protein binding	2	1
GO:0019899	enzyme binding	3	1
GO:0031624	ubiquitin conjugating enzyme binding	5	1
GO:0044390	ubiquitin-like protein conjugating enzyme binding	4	1
GO:0061630	ubiquitin protein ligase activity	5	1
GO:0061659	ubiquitin-like protein ligase activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	355	359	PF00656	0.562
CLV_NRD_NRD_1	211	213	PF00675	0.352
CLV_NRD_NRD_1	361	363	PF00675	0.463
CLV_PCSK_KEX2_1	361	363	PF00082	0.494
CLV_PCSK_SKI1_1	204	208	PF00082	0.483
CLV_PCSK_SKI1_1	213	217	PF00082	0.263
CLV_PCSK_SKI1_1	251	255	PF00082	0.399
CLV_PCSK_SKI1_1	269	273	PF00082	0.328
CLV_PCSK_SKI1_1	361	365	PF00082	0.416
CLV_PCSK_SKI1_1	396	400	PF00082	0.585
CLV_PCSK_SKI1_1	446	450	PF00082	0.375
CLV_PCSK_SKI1_1	489	493	PF00082	0.546
DEG_Nend_Nbox_1	1	3	PF02207	0.586
DEG_SIAH_1	24	32	PF03145	0.441
DOC_CDC14_PxL_1	239	247	PF14671	0.401
DOC_MAPK_gen_1	82	91	PF00069	0.445
DOC_USP7_MATH_1	31	35	PF00917	0.652
DOC_USP7_MATH_1	350	354	PF00917	0.512
DOC_USP7_UBL2_3	204	208	PF12436	0.378
DOC_USP7_UBL2_3	305	309	PF12436	0.355
DOC_WW_Pin1_4	69	74	PF00397	0.222
LIG_14-3-3_CanoR_1	262	266	PF00244	0.400
LIG_14-3-3_CanoR_1	269	276	PF00244	0.396
LIG_14-3-3_CanoR_1	277	283	PF00244	0.471
LIG_14-3-3_CanoR_1	361	366	PF00244	0.525
LIG_14-3-3_CanoR_1	369	373	PF00244	0.424
LIG_14-3-3_CanoR_1	437	443	PF00244	0.496
LIG_Actin_WH2_2	246	264	PF00022	0.401
LIG_APCC_ABBAyCdc20_2	369	375	PF00400	0.467
LIG_BIR_III_4	122	126	PF00653	0.279
LIG_BRCT_BRCA1_1	359	363	PF00533	0.521
LIG_deltaCOP1_diTrp_1	323	332	PF00928	0.309
LIG_FHA_1	115	121	PF00498	0.279
LIG_FHA_1	214	220	PF00498	0.396
LIG_FHA_1	369	375	PF00498	0.449
LIG_FHA_1	429	435	PF00498	0.445
LIG_FHA_1	437	443	PF00498	0.379
LIG_FHA_1	75	81	PF00498	0.429
LIG_FHA_1	97	103	PF00498	0.524
LIG_FHA_2	390	396	PF00498	0.601
LIG_FHA_2	442	448	PF00498	0.465
LIG_LIR_Gen_1	323	331	PF02991	0.307
LIG_LIR_Gen_1	452	459	PF02991	0.444
LIG_LIR_Nem_3	323	329	PF02991	0.307
LIG_LIR_Nem_3	364	370	PF02991	0.487
LIG_PTB_Apo_2	253	260	PF02174	0.401
LIG_PTB_Phospho_1	253	259	PF10480	0.401
LIG_SH2_CRK	239	243	PF00017	0.401
LIG_SH2_NCK_1	239	243	PF00017	0.401
LIG_SH2_SRC	2	5	PF00017	0.648
LIG_SH2_SRC	239	242	PF00017	0.401
LIG_SH2_SRC	265	268	PF00017	0.401
LIG_SH2_STAP1	370	374	PF00017	0.467
LIG_SH2_STAT3	136	139	PF00017	0.400
LIG_SH2_STAT3	20	23	PF00017	0.706
LIG_SH2_STAT5	150	153	PF00017	0.427
LIG_SH2_STAT5	214	217	PF00017	0.298
LIG_SH2_STAT5	259	262	PF00017	0.401
LIG_SH2_STAT5	282	285	PF00017	0.488
LIG_SH2_STAT5	292	295	PF00017	0.494
LIG_SH2_STAT5	370	373	PF00017	0.414
LIG_SH2_STAT5	453	456	PF00017	0.339
LIG_SH2_STAT5	458	461	PF00017	0.419
LIG_SH2_STAT5	94	97	PF00017	0.409
LIG_SH3_3	238	244	PF00018	0.357
LIG_SH3_3	264	270	PF00018	0.285
LIG_SH3_3	385	391	PF00018	0.469
LIG_Sin3_3	116	123	PF02671	0.248
LIG_Sin3_3	26	33	PF02671	0.435
LIG_SUMO_SIM_par_1	115	122	PF11976	0.459
LIG_SUMO_SIM_par_1	423	428	PF11976	0.473
LIG_TRAF2_1	19	22	PF00917	0.611
LIG_TRAF2_1	352	355	PF00917	0.686
LIG_TYR_ITIM	237	242	PF00017	0.401
LIG_UBA3_1	206	213	PF00899	0.401
LIG_WRC_WIRS_1	215	220	PF05994	0.377
MOD_CK1_1	281	287	PF00069	0.506
MOD_CK1_1	436	442	PF00069	0.474
MOD_CK1_1	74	80	PF00069	0.447
MOD_CK2_1	109	115	PF00069	0.518
MOD_CK2_1	214	220	PF00069	0.474
MOD_CK2_1	389	395	PF00069	0.561
MOD_GlcNHglycan	308	312	PF01048	0.397
MOD_GlcNHglycan	31	34	PF01048	0.662
MOD_GlcNHglycan	335	338	PF01048	0.574
MOD_GlcNHglycan	375	378	PF01048	0.483
MOD_GlcNHglycan	413	416	PF01048	0.746
MOD_GlcNHglycan	483	486	PF01048	0.476
MOD_GlcNHglycan	73	76	PF01048	0.459
MOD_GSK3_1	105	112	PF00069	0.405
MOD_GSK3_1	214	221	PF00069	0.323
MOD_GSK3_1	303	310	PF00069	0.411
MOD_GSK3_1	357	364	PF00069	0.562
MOD_GSK3_1	425	432	PF00069	0.455
MOD_GSK3_1	45	52	PF00069	0.461
MOD_GSK3_1	477	484	PF00069	0.396
MOD_GSK3_1	489	496	PF00069	0.448
MOD_GSK3_1	96	103	PF00069	0.492
MOD_N-GLC_1	255	260	PF02516	0.338
MOD_N-GLC_1	461	466	PF02516	0.466
MOD_N-GLC_1	66	71	PF02516	0.462
MOD_N-GLC_1	96	101	PF02516	0.497
MOD_NEK2_1	168	173	PF00069	0.419
MOD_NEK2_1	218	223	PF00069	0.396
MOD_NEK2_1	294	299	PF00069	0.398
MOD_NEK2_1	383	388	PF00069	0.555
MOD_NEK2_1	449	454	PF00069	0.389
MOD_NEK2_1	483	488	PF00069	0.490
MOD_NEK2_1	490	495	PF00069	0.408
MOD_NEK2_1	95	100	PF00069	0.492
MOD_OFUCOSY	313	318	PF10250	0.309
MOD_PIKK_1	493	499	PF00454	0.566
MOD_PKA_1	361	367	PF00069	0.494
MOD_PKA_2	261	267	PF00069	0.461
MOD_PKA_2	361	367	PF00069	0.467
MOD_PKA_2	368	374	PF00069	0.432
MOD_PKA_2	436	442	PF00069	0.506
MOD_PKA_2	83	89	PF00069	0.463
MOD_Plk_1	109	115	PF00069	0.560
MOD_Plk_1	255	261	PF00069	0.425
MOD_Plk_1	45	51	PF00069	0.496
MOD_Plk_1	66	72	PF00069	0.468
MOD_Plk_1	96	102	PF00069	0.431
MOD_Plk_2-3	109	115	PF00069	0.488
MOD_Plk_2-3	189	195	PF00069	0.352
MOD_Plk_2-3	45	51	PF00069	0.512
MOD_Plk_4	114	120	PF00069	0.333
MOD_Plk_4	182	188	PF00069	0.337
MOD_Plk_4	214	220	PF00069	0.384
MOD_Plk_4	438	444	PF00069	0.422
MOD_Plk_4	449	455	PF00069	0.335
MOD_Plk_4	483	489	PF00069	0.501
MOD_Plk_4	74	80	PF00069	0.406
MOD_ProDKin_1	69	75	PF00069	0.218
MOD_SUMO_rev_2	375	384	PF00179	0.449
TRG_DiLeu_BaEn_1	115	120	PF01217	0.238
TRG_DiLeu_BaLyEn_6	393	398	PF01217	0.502
TRG_ENDOCYTIC_2	150	153	PF00928	0.425
TRG_ENDOCYTIC_2	239	242	PF00928	0.401
TRG_ENDOCYTIC_2	453	456	PF00928	0.339
TRG_ER_diArg_1	361	363	PF00400	0.466
TRG_Pf-PMV_PEXEL_1	402	406	PF00026	0.424

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8P3	Leptomonas seymouri	50%	100%
A0A0S4JJP0	Bodo saltans	27%	100%
A0A1X0P1P5	Trypanosomatidae	26%	100%
A0A3Q8IEV4	Leishmania donovani	92%	98%
A0A3R7JXL5	Trypanosoma rangeli	29%	100%
A2VEA3	Bos taurus	28%	93%
A4HIE9	Leishmania braziliensis	80%	100%
A4I5P6	Leishmania infantum	93%	98%
B1H1E4	Xenopus tropicalis	28%	98%
C9ZR47	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	30%	100%
O01965	Caenorhabditis elegans	27%	100%
O76924	Drosophila melanogaster	25%	100%
O95376	Homo sapiens	27%	100%
P0C8K8	Arabidopsis thaliana	27%	94%
P36113	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	24%	94%
Q22431	Caenorhabditis elegans	26%	100%
Q32NS4	Xenopus laevis	27%	98%
Q6NW85	Danio rerio	29%	97%
Q6PFJ9	Danio rerio	28%	98%
Q6T486	Dictyostelium discoideum	26%	100%
Q84RQ8	Arabidopsis thaliana	26%	100%
Q84RQ9	Arabidopsis thaliana	24%	100%
Q84RR0	Arabidopsis thaliana	28%	92%
Q84RR2	Arabidopsis thaliana	25%	87%
Q8L829	Arabidopsis thaliana	26%	94%
Q8W468	Arabidopsis thaliana	28%	91%
Q94981	Drosophila melanogaster	26%	100%
Q949V6	Arabidopsis thaliana	25%	87%
Q9LVW9	Arabidopsis thaliana	27%	98%
Q9LVX0	Arabidopsis thaliana	25%	96%
Q9NE54	Leishmania major	93%	100%
Q9P3U4	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	27%	100%
Q9SKC3	Arabidopsis thaliana	26%	95%
Q9SKC4	Arabidopsis thaliana	28%	100%
Q9Y4X5	Homo sapiens	28%	93%
Q9Z1K5	Mus musculus	28%	93%
Q9Z1K6	Mus musculus	26%	100%
V5AQL7	Trypanosoma cruzi	30%	100%