tRNA pseudouridine synthase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

pseudouridine synthase A-like protein, putative

Species:

Leishmania mexicana

UniProt:

E9B0S9_LEIMU

TriTrypDb:

LmxM.29.1550

Length:

499

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0005737	cytoplasm	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9B0S9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B0S9

Function

Biological processes
Term	Name	Level	Count
GO:0001522	pseudouridine synthesis	6	11
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006396	RNA processing	6	12
GO:0006399	tRNA metabolic process	7	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008033	tRNA processing	8	12
GO:0008152	metabolic process	1	12
GO:0009451	RNA modification	5	11
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0034470	ncRNA processing	7	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034660	ncRNA metabolic process	6	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	11
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0006400	tRNA modification	6	1
GO:0016071	mRNA metabolic process	6	1
GO:0016556	mRNA modification	6	1
GO:0031119	tRNA pseudouridine synthesis	7	1
GO:1990481	mRNA pseudouridine synthesis	7	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0009982	pseudouridine synthase activity	4	12
GO:0016829	lyase activity	2	7
GO:0016853	isomerase activity	2	12
GO:0016866	intramolecular transferase activity	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0106029	tRNA pseudouridine synthase activity	5	9
GO:0140098	catalytic activity, acting on RNA	3	9
GO:0140101	catalytic activity, acting on a tRNA	4	9
GO:0140640	catalytic activity, acting on a nucleic acid	2	9
GO:1901363	heterocyclic compound binding	2	12
GO:0004730	pseudouridylate synthase activity	5	1
GO:0016835	carbon-oxygen lyase activity	3	1
GO:0016836	hydro-lyase activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	5	9	PF00656	0.747
CLV_NRD_NRD_1	355	357	PF00675	0.620
CLV_NRD_NRD_1	37	39	PF00675	0.534
CLV_NRD_NRD_1	376	378	PF00675	0.548
CLV_NRD_NRD_1	382	384	PF00675	0.555
CLV_NRD_NRD_1	405	407	PF00675	0.429
CLV_NRD_NRD_1	59	61	PF00675	0.344
CLV_NRD_NRD_1	95	97	PF00675	0.436
CLV_PCSK_KEX2_1	362	364	PF00082	0.375
CLV_PCSK_KEX2_1	376	378	PF00082	0.548
CLV_PCSK_KEX2_1	382	384	PF00082	0.555
CLV_PCSK_KEX2_1	407	409	PF00082	0.464
CLV_PCSK_KEX2_1	474	476	PF00082	0.399
CLV_PCSK_KEX2_1	95	97	PF00082	0.383
CLV_PCSK_PC1ET2_1	362	364	PF00082	0.375
CLV_PCSK_PC1ET2_1	407	409	PF00082	0.521
CLV_PCSK_PC1ET2_1	474	476	PF00082	0.399
CLV_PCSK_PC7_1	372	378	PF00082	0.474
CLV_PCSK_SKI1_1	224	228	PF00082	0.311
CLV_PCSK_SKI1_1	357	361	PF00082	0.498
CLV_PCSK_SKI1_1	408	412	PF00082	0.536
CLV_PCSK_SKI1_1	50	54	PF00082	0.474
CLV_PCSK_SKI1_1	61	65	PF00082	0.360
DEG_APCC_DBOX_1	407	415	PF00400	0.513
DEG_SPOP_SBC_1	2	6	PF00917	0.745
DEG_SPOP_SBC_1	423	427	PF00917	0.575
DOC_CKS1_1	17	22	PF01111	0.433
DOC_CYCLIN_RxL_1	92	103	PF00134	0.346
DOC_MAPK_gen_1	60	66	PF00069	0.287
DOC_MAPK_MEF2A_6	257	264	PF00069	0.258
DOC_PP2B_LxvP_1	270	273	PF13499	0.323
DOC_USP7_MATH_1	129	133	PF00917	0.450
DOC_USP7_MATH_1	2	6	PF00917	0.625
DOC_USP7_MATH_1	339	343	PF00917	0.518
DOC_WW_Pin1_4	16	21	PF00397	0.499
DOC_WW_Pin1_4	292	297	PF00397	0.288
DOC_WW_Pin1_4	30	35	PF00397	0.665
LIG_14-3-3_CanoR_1	110	118	PF00244	0.336
LIG_14-3-3_CanoR_1	130	139	PF00244	0.345
LIG_14-3-3_CanoR_1	173	181	PF00244	0.284
LIG_14-3-3_CanoR_1	224	230	PF00244	0.349
LIG_14-3-3_CanoR_1	247	252	PF00244	0.302
LIG_14-3-3_CanoR_1	257	261	PF00244	0.206
LIG_14-3-3_CanoR_1	333	340	PF00244	0.402
LIG_Actin_WH2_2	82	100	PF00022	0.402
LIG_BIR_II_1	1	5	PF00653	0.734
LIG_BIR_III_2	136	140	PF00653	0.553
LIG_BRCT_BRCA1_1	341	345	PF00533	0.424
LIG_deltaCOP1_diTrp_1	385	394	PF00928	0.441
LIG_FHA_1	155	161	PF00498	0.296
LIG_FHA_1	257	263	PF00498	0.172
LIG_FHA_1	286	292	PF00498	0.326
LIG_FHA_1	293	299	PF00498	0.318
LIG_FHA_1	326	332	PF00498	0.397
LIG_FHA_1	349	355	PF00498	0.428
LIG_FHA_1	447	453	PF00498	0.382
LIG_FHA_2	276	282	PF00498	0.385
LIG_FHA_2	388	394	PF00498	0.469
LIG_FHA_2	426	432	PF00498	0.724
LIG_FHA_2	460	466	PF00498	0.400
LIG_LIR_Apic_2	393	397	PF02991	0.466
LIG_LIR_Gen_1	145	154	PF02991	0.337
LIG_LIR_Gen_1	171	181	PF02991	0.343
LIG_LIR_Gen_1	242	251	PF02991	0.261
LIG_LIR_Gen_1	301	311	PF02991	0.449
LIG_LIR_Nem_3	171	177	PF02991	0.304
LIG_LIR_Nem_3	180	186	PF02991	0.252
LIG_LIR_Nem_3	205	211	PF02991	0.340
LIG_LIR_Nem_3	242	246	PF02991	0.242
LIG_LIR_Nem_3	250	256	PF02991	0.242
LIG_LIR_Nem_3	301	307	PF02991	0.278
LIG_LIR_Nem_3	342	348	PF02991	0.437
LIG_LIR_Nem_3	449	454	PF02991	0.320
LIG_MYND_1	139	143	PF01753	0.464
LIG_Pex14_1	300	304	PF04695	0.242
LIG_Pex14_2	170	174	PF04695	0.291
LIG_REV1ctd_RIR_1	183	192	PF16727	0.336
LIG_SH2_CRK	253	257	PF00017	0.242
LIG_SH2_PTP2	451	454	PF00017	0.441
LIG_SH2_SRC	243	246	PF00017	0.336
LIG_SH2_STAP1	463	467	PF00017	0.513
LIG_SH2_STAT5	146	149	PF00017	0.290
LIG_SH2_STAT5	184	187	PF00017	0.421
LIG_SH2_STAT5	243	246	PF00017	0.258
LIG_SH2_STAT5	348	351	PF00017	0.478
LIG_SH2_STAT5	413	416	PF00017	0.590
LIG_SH2_STAT5	451	454	PF00017	0.381
LIG_SH3_3	136	142	PF00018	0.477
LIG_SH3_3	14	20	PF00018	0.592
LIG_SH3_3	284	290	PF00018	0.242
LIG_SH3_3	442	448	PF00018	0.750
LIG_SH3_5	142	146	PF00018	0.350
LIG_SUMO_SIM_par_1	278	284	PF11976	0.381
LIG_SUMO_SIM_par_1	295	301	PF11976	0.197
LIG_TRAF2_1	194	197	PF00917	0.429
LIG_TRAF2_1	233	236	PF00917	0.307
LIG_TRAF2_1	429	432	PF00917	0.599
LIG_TRAF2_1	462	465	PF00917	0.478
LIG_TYR_ITIM	241	246	PF00017	0.258
LIG_UBA3_1	279	286	PF00899	0.248
MOD_CK1_1	26	32	PF00069	0.622
MOD_CK1_1	3	9	PF00069	0.720
MOD_CK1_1	425	431	PF00069	0.626
MOD_CK2_1	239	245	PF00069	0.251
MOD_CK2_1	275	281	PF00069	0.385
MOD_CK2_1	425	431	PF00069	0.737
MOD_CK2_1	432	438	PF00069	0.647
MOD_CK2_1	459	465	PF00069	0.385
MOD_Cter_Amidation	471	474	PF01082	0.537
MOD_GlcNHglycan	10	13	PF01048	0.602
MOD_GlcNHglycan	118	121	PF01048	0.333
MOD_GlcNHglycan	335	338	PF01048	0.487
MOD_GlcNHglycan	365	368	PF01048	0.605
MOD_GlcNHglycan	442	445	PF01048	0.681
MOD_GlcNHglycan	469	473	PF01048	0.470
MOD_GSK3_1	125	132	PF00069	0.309
MOD_GSK3_1	19	26	PF00069	0.730
MOD_GSK3_1	271	278	PF00069	0.406
MOD_GSK3_1	392	399	PF00069	0.521
MOD_GSK3_1	4	11	PF00069	0.578
MOD_GSK3_1	422	429	PF00069	0.627
MOD_GSK3_1	434	441	PF00069	0.650
MOD_N-GLC_1	217	222	PF02516	0.280
MOD_N-GLC_2	210	212	PF02516	0.242
MOD_NEK2_1	1	6	PF00069	0.726
MOD_NEK2_1	125	130	PF00069	0.358
MOD_NEK2_1	202	207	PF00069	0.336
MOD_NEK2_1	222	227	PF00069	0.110
MOD_NEK2_1	40	45	PF00069	0.681
MOD_NEK2_1	424	429	PF00069	0.636
MOD_PIKK_1	339	345	PF00454	0.431
MOD_PIKK_1	432	438	PF00454	0.771
MOD_PKA_2	129	135	PF00069	0.325
MOD_PKA_2	172	178	PF00069	0.290
MOD_PKA_2	256	262	PF00069	0.199
MOD_PKA_2	26	32	PF00069	0.482
MOD_PKA_2	332	338	PF00069	0.385
MOD_PKA_2	456	462	PF00069	0.389
MOD_Plk_1	459	465	PF00069	0.396
MOD_Plk_1	82	88	PF00069	0.458
MOD_Plk_2-3	239	245	PF00069	0.242
MOD_Plk_4	145	151	PF00069	0.372
MOD_Plk_4	247	253	PF00069	0.290
MOD_Plk_4	271	277	PF00069	0.343
MOD_ProDKin_1	16	22	PF00069	0.499
MOD_ProDKin_1	292	298	PF00069	0.288
MOD_ProDKin_1	30	36	PF00069	0.665
MOD_SUMO_rev_2	281	288	PF00179	0.247
MOD_SUMO_rev_2	425	435	PF00179	0.622
MOD_SUMO_rev_2	479	486	PF00179	0.441
TRG_DiLeu_BaEn_1	236	241	PF01217	0.247
TRG_DiLeu_BaEn_1	261	266	PF01217	0.258
TRG_DiLeu_BaEn_4	465	471	PF01217	0.400
TRG_DiLeu_BaLyEn_6	450	455	PF01217	0.557
TRG_DiLeu_BaLyEn_6	95	100	PF01217	0.323
TRG_ENDOCYTIC_2	146	149	PF00928	0.343
TRG_ENDOCYTIC_2	183	186	PF00928	0.427
TRG_ENDOCYTIC_2	243	246	PF00928	0.250
TRG_ENDOCYTIC_2	253	256	PF00928	0.230
TRG_ENDOCYTIC_2	451	454	PF00928	0.381
TRG_ER_diArg_1	375	377	PF00400	0.565
TRG_ER_diArg_1	381	383	PF00400	0.572
TRG_ER_diArg_1	475	478	PF00400	0.391
TRG_ER_diArg_1	94	96	PF00400	0.334
TRG_NLS_MonoExtC_3	43	49	PF00514	0.483
TRG_NLS_MonoExtC_3	472	477	PF00514	0.401
TRG_NLS_MonoExtN_4	473	478	PF00514	0.399
TRG_Pf-PMV_PEXEL_1	98	102	PF00026	0.386

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IA61	Leptomonas seymouri	72%	97%
A0A0S4JB03	Bodo saltans	51%	100%
A0A1X0P1U9	Trypanosomatidae	58%	100%
A0A3S7X3E8	Leishmania donovani	93%	100%
A0A422P3K1	Trypanosoma rangeli	57%	100%
A4HIA0	Leishmania braziliensis	83%	100%
A4I5I4	Leishmania infantum	93%	100%
C9ZQW8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	54%	100%
Q09524	Caenorhabditis elegans	34%	100%
Q3SX07	Bos taurus	37%	100%
Q4Q7E9	Leishmania major	91%	100%
V5DQN1	Trypanosoma cruzi	60%	95%