RF_PROK_I domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

RF-1 domain containing protein, putative

Species:

Leishmania mexicana

UniProt:

E9B0N5_LEIMU

TriTrypDb:

LmxM.29.1060

Length:

179

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0000315	organellar large ribosomal subunit	5	1
GO:0005762	mitochondrial large ribosomal subunit	3	1
GO:0015934	large ribosomal subunit	4	1
GO:0032991	protein-containing complex	1	1
GO:0044391	ribosomal subunit	3	1
GO:0098798	mitochondrial protein-containing complex	2	1
GO:1990904	ribonucleoprotein complex	2	1

Expansion

Sequence features

E9B0N5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B0N5

Function

Biological processes
Term	Name	Level	Count
GO:0006415	translational termination	6	1
GO:0009987	cellular process	1	1
GO:0016043	cellular component organization	3	1
GO:0022411	cellular component disassembly	4	1
GO:0032984	protein-containing complex disassembly	5	1
GO:0043933	protein-containing complex organization	4	1
GO:0070126	mitochondrial translational termination	7	1
GO:0071840	cellular component organization or biogenesis	2	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	11
GO:0003747	translation release factor activity	5	11
GO:0005488	binding	1	11
GO:0008079	translation termination factor activity	4	11
GO:0008135	translation factor activity, RNA binding	3	11
GO:0045182	translation regulator activity	1	11
GO:0090079	translation regulator activity, nucleic acid binding	2	11
GO:0097159	organic cyclic compound binding	2	11
GO:1901363	heterocyclic compound binding	2	11
GO:0003824	catalytic activity	1	1
GO:0004045	aminoacyl-tRNA hydrolase activity	5	1
GO:0016150	translation release factor activity, codon nonspecific	6	1
GO:0016787	hydrolase activity	2	1
GO:0016788	hydrolase activity, acting on ester bonds	3	1
GO:0052689	carboxylic ester hydrolase activity	4	1
GO:0140098	catalytic activity, acting on RNA	3	1
GO:0140101	catalytic activity, acting on a tRNA	4	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	126	128	PF00675	0.259
CLV_NRD_NRD_1	161	163	PF00675	0.559
CLV_NRD_NRD_1	93	95	PF00675	0.352
CLV_PCSK_KEX2_1	160	162	PF00082	0.563
CLV_PCSK_KEX2_1	93	95	PF00082	0.331
CLV_PCSK_SKI1_1	116	120	PF00082	0.367
DEG_Nend_Nbox_1	1	3	PF02207	0.413
DOC_ANK_TNKS_1	54	61	PF00023	0.436
DOC_USP7_MATH_1	100	104	PF00917	0.474
DOC_USP7_MATH_1	21	25	PF00917	0.545
LIG_14-3-3_CanoR_1	127	133	PF00244	0.542
LIG_14-3-3_CanoR_1	135	139	PF00244	0.591
LIG_14-3-3_CanoR_1	169	179	PF00244	0.592
LIG_BRCT_BRCA1_1	80	84	PF00533	0.449
LIG_FHA_1	104	110	PF00498	0.492
LIG_FHA_1	9	15	PF00498	0.627
LIG_LIR_Apic_2	137	141	PF02991	0.530
LIG_LIR_Nem_3	30	36	PF02991	0.568
LIG_MLH1_MIPbox_1	80	84	PF16413	0.492
LIG_NRBOX	128	134	PF00104	0.531
LIG_PCNA_PIPBox_1	77	86	PF02747	0.492
LIG_SH2_STAT3	34	37	PF00017	0.577
LIG_SH3_3	144	150	PF00018	0.591
LIG_SUMO_SIM_par_1	106	111	PF11976	0.451
MOD_CK1_1	103	109	PF00069	0.508
MOD_CK1_1	173	179	PF00069	0.686
MOD_CK1_1	24	30	PF00069	0.492
MOD_CK2_1	108	114	PF00069	0.454
MOD_CK2_1	13	19	PF00069	0.719
MOD_CK2_1	24	30	PF00069	0.612
MOD_GlcNHglycan	116	119	PF01048	0.259
MOD_GlcNHglycan	172	175	PF01048	0.631
MOD_GlcNHglycan	56	59	PF01048	0.310
MOD_GSK3_1	148	155	PF00069	0.536
MOD_GSK3_1	164	171	PF00069	0.572
MOD_GSK3_1	21	28	PF00069	0.471
MOD_GSK3_1	50	57	PF00069	0.482
MOD_GSK3_1	61	68	PF00069	0.462
MOD_GSK3_1	9	16	PF00069	0.585
MOD_GSK3_1	99	106	PF00069	0.524
MOD_N-GLC_1	64	69	PF02516	0.221
MOD_NEK2_1	170	175	PF00069	0.532
MOD_NEK2_1	54	59	PF00069	0.533
MOD_NEK2_1	9	14	PF00069	0.670
MOD_PKA_1	162	168	PF00069	0.608
MOD_PKA_2	134	140	PF00069	0.639
MOD_PKA_2	148	154	PF00069	0.626
MOD_PKA_2	168	174	PF00069	0.410
MOD_PKA_2	21	27	PF00069	0.570
MOD_PKA_2	54	60	PF00069	0.494
MOD_PKB_1	160	168	PF00069	0.615
MOD_Plk_4	104	110	PF00069	0.531
MOD_Plk_4	128	134	PF00069	0.453
MOD_Plk_4	148	154	PF00069	0.436
MOD_Plk_4	9	15	PF00069	0.480
MOD_SUMO_rev_2	151	159	PF00179	0.697
TRG_DiLeu_BaEn_2	147	153	PF01217	0.582
TRG_ENDOCYTIC_2	33	36	PF00928	0.570
TRG_ER_diArg_1	159	162	PF00400	0.600
TRG_ER_diArg_1	92	94	PF00400	0.529
TRG_NLS_MonoExtN_4	160	166	PF00514	0.559

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I3M0	Leptomonas seymouri	77%	100%
A0A0S4IXK6	Bodo saltans	49%	85%
A0A3S7X372	Leishmania donovani	94%	89%
A0A422P522	Trypanosoma rangeli	53%	69%
A4HI61	Leishmania braziliensis	82%	100%
A4I5D7	Leishmania infantum	94%	89%
C9ZQR8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	53%	79%
Q4Q7J7	Leishmania major	95%	100%
V5BZ05	Trypanosoma cruzi	55%	68%