Spindle pole body component

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Gamma-tubulin complex component 4

Species:

Leishmania mexicana

UniProt:

E9B0M3_LEIMU

TriTrypDb:

LmxM.29.0940

Length:

784

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0000922	spindle pole	2	11
GO:0005737	cytoplasm	2	11
GO:0005815	microtubule organizing center	2	11
GO:0005874	microtubule	6	11
GO:0099080	supramolecular complex	2	11
GO:0099081	supramolecular polymer	3	11
GO:0099512	supramolecular fiber	4	11
GO:0099513	polymeric cytoskeletal fiber	5	11
GO:0110165	cellular anatomical entity	1	11
GO:0000923	equatorial microtubule organizing center	3	1
GO:0000930	gamma-tubulin complex	2	1
GO:0005813	centrosome	3	1
GO:0032991	protein-containing complex	1	1
GO:0036064	ciliary basal body	3	1

Expansion

Sequence features

E9B0M3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B0M3

Function

Biological processes
Term	Name	Level	Count
GO:0000226	microtubule cytoskeleton organization	3	11
GO:0006996	organelle organization	4	11
GO:0007010	cytoskeleton organization	5	11
GO:0007017	microtubule-based process	2	11
GO:0007020	microtubule nucleation	4	11
GO:0009987	cellular process	1	11
GO:0016043	cellular component organization	3	11
GO:0071840	cellular component organization or biogenesis	2	11
GO:0000278	mitotic cell cycle	3	1
GO:0007049	cell cycle	2	1
GO:0007051	spindle organization	3	1
GO:0022402	cell cycle process	2	1
GO:0022414	reproductive process	1	1
GO:0022607	cellular component assembly	4	1
GO:0031122	cytoplasmic microtubule organization	4	1
GO:0051225	spindle assembly	4	1
GO:0051321	meiotic cell cycle	2	1
GO:0070925	organelle assembly	5	1
GO:0097435	supramolecular fiber organization	4	1
GO:0140694	non-membrane-bounded organelle assembly	6	1

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	11
GO:0005515	protein binding	2	11
GO:0008092	cytoskeletal protein binding	3	11
GO:0015631	tubulin binding	4	11
GO:0043015	gamma-tubulin binding	5	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	25	29	PF00656	0.626
CLV_C14_Caspase3-7	35	39	PF00656	0.628
CLV_MEL_PAP_1	540	546	PF00089	0.249
CLV_NRD_NRD_1	168	170	PF00675	0.497
CLV_NRD_NRD_1	258	260	PF00675	0.370
CLV_NRD_NRD_1	50	52	PF00675	0.462
CLV_NRD_NRD_1	533	535	PF00675	0.189
CLV_NRD_NRD_1	571	573	PF00675	0.254
CLV_NRD_NRD_1	779	781	PF00675	0.709
CLV_PCSK_KEX2_1	168	170	PF00082	0.497
CLV_PCSK_KEX2_1	258	260	PF00082	0.370
CLV_PCSK_KEX2_1	50	52	PF00082	0.462
CLV_PCSK_KEX2_1	533	535	PF00082	0.189
CLV_PCSK_KEX2_1	571	573	PF00082	0.254
CLV_PCSK_KEX2_1	619	621	PF00082	0.250
CLV_PCSK_KEX2_1	779	781	PF00082	0.715
CLV_PCSK_PC1ET2_1	619	621	PF00082	0.250
CLV_PCSK_SKI1_1	104	108	PF00082	0.456
CLV_PCSK_SKI1_1	168	172	PF00082	0.431
CLV_PCSK_SKI1_1	258	262	PF00082	0.337
CLV_PCSK_SKI1_1	30	34	PF00082	0.516
CLV_PCSK_SKI1_1	342	346	PF00082	0.236
CLV_PCSK_SKI1_1	501	505	PF00082	0.236
CLV_PCSK_SKI1_1	533	537	PF00082	0.348
CLV_PCSK_SKI1_1	553	557	PF00082	0.184
CLV_PCSK_SKI1_1	571	575	PF00082	0.236
DEG_APCC_DBOX_1	500	508	PF00400	0.449
DEG_APCC_DBOX_1	542	550	PF00400	0.418
DEG_APCC_DBOX_1	680	688	PF00400	0.531
DEG_Nend_UBRbox_2	1	3	PF02207	0.373
DOC_CKS1_1	20	25	PF01111	0.410
DOC_CYCLIN_RxL_1	102	112	PF00134	0.335
DOC_CYCLIN_RxL_1	165	174	PF00134	0.452
DOC_CYCLIN_RxL_1	498	508	PF00134	0.577
DOC_CYCLIN_RxL_1	550	558	PF00134	0.539
DOC_CYCLIN_RxL_1	65	75	PF00134	0.479
DOC_MAPK_DCC_7	327	336	PF00069	0.394
DOC_MAPK_gen_1	50	58	PF00069	0.454
DOC_MAPK_HePTP_8	324	336	PF00069	0.433
DOC_MAPK_MEF2A_6	146	153	PF00069	0.400
DOC_MAPK_MEF2A_6	327	336	PF00069	0.438
DOC_PP1_RVXF_1	102	109	PF00149	0.336
DOC_PP1_RVXF_1	715	721	PF00149	0.436
DOC_USP7_MATH_1	216	220	PF00917	0.723
DOC_USP7_MATH_1	278	282	PF00917	0.553
DOC_USP7_MATH_1	32	36	PF00917	0.657
DOC_USP7_MATH_1	418	422	PF00917	0.535
DOC_USP7_MATH_1	425	429	PF00917	0.541
DOC_USP7_MATH_1	611	615	PF00917	0.509
DOC_USP7_MATH_1	645	649	PF00917	0.461
DOC_USP7_MATH_1	736	740	PF00917	0.639
DOC_USP7_MATH_1	761	765	PF00917	0.721
DOC_WW_Pin1_4	116	121	PF00397	0.450
DOC_WW_Pin1_4	19	24	PF00397	0.571
DOC_WW_Pin1_4	241	246	PF00397	0.367
DOC_WW_Pin1_4	378	383	PF00397	0.535
DOC_WW_Pin1_4	673	678	PF00397	0.544
LIG_14-3-3_CanoR_1	433	441	PF00244	0.451
LIG_14-3-3_CanoR_1	543	547	PF00244	0.465
LIG_14-3-3_CanoR_1	553	561	PF00244	0.551
LIG_14-3-3_CanoR_1	571	577	PF00244	0.416
LIG_14-3-3_CanoR_1	644	650	PF00244	0.482
LIG_14-3-3_CanoR_1	681	691	PF00244	0.503
LIG_14-3-3_CanoR_1	74	80	PF00244	0.439
LIG_14-3-3_CanoR_1	754	761	PF00244	0.756
LIG_14-3-3_CanoR_1	763	771	PF00244	0.744
LIG_14-3-3_CanoR_1	81	88	PF00244	0.400
LIG_Actin_WH2_2	436	451	PF00022	0.517
LIG_AP2alpha_1	621	625	PF02296	0.482
LIG_BRCT_BRCA1_1	220	224	PF00533	0.564
LIG_BRCT_BRCA1_1	435	439	PF00533	0.451
LIG_Clathr_ClatBox_1	504	508	PF01394	0.451
LIG_deltaCOP1_diTrp_1	351	356	PF00928	0.436
LIG_DLG_GKlike_1	572	579	PF00625	0.435
LIG_FHA_1	127	133	PF00498	0.362
LIG_FHA_1	212	218	PF00498	0.661
LIG_FHA_1	234	240	PF00498	0.432
LIG_FHA_1	456	462	PF00498	0.494
LIG_FHA_1	494	500	PF00498	0.546
LIG_FHA_1	550	556	PF00498	0.531
LIG_FHA_1	598	604	PF00498	0.463
LIG_FHA_1	698	704	PF00498	0.425
LIG_FHA_2	133	139	PF00498	0.378
LIG_FHA_2	611	617	PF00498	0.482
LIG_FHA_2	674	680	PF00498	0.533
LIG_GBD_Chelix_1	499	507	PF00786	0.165
LIG_Integrin_RGD_1	349	351	PF01839	0.236
LIG_LIR_Gen_1	133	141	PF02991	0.403
LIG_LIR_Gen_1	173	184	PF02991	0.379
LIG_LIR_Gen_1	351	362	PF02991	0.436
LIG_LIR_Gen_1	451	461	PF02991	0.517
LIG_LIR_Gen_1	575	583	PF02991	0.471
LIG_LIR_Gen_1	75	82	PF02991	0.537
LIG_LIR_Nem_3	133	137	PF02991	0.368
LIG_LIR_Nem_3	173	179	PF02991	0.309
LIG_LIR_Nem_3	198	203	PF02991	0.338
LIG_LIR_Nem_3	351	357	PF02991	0.436
LIG_LIR_Nem_3	451	457	PF02991	0.531
LIG_LIR_Nem_3	508	514	PF02991	0.547
LIG_LIR_Nem_3	530	535	PF02991	0.408
LIG_LIR_Nem_3	575	580	PF02991	0.531
LIG_LIR_Nem_3	719	723	PF02991	0.418
LIG_LIR_Nem_3	75	79	PF02991	0.497
LIG_MYND_1	389	393	PF01753	0.443
LIG_NRBOX	575	581	PF00104	0.441
LIG_PCNA_yPIPBox_3	592	602	PF02747	0.451
LIG_PCNA_yPIPBox_3	690	699	PF02747	0.532
LIG_Pex14_2	290	294	PF04695	0.463
LIG_Pex14_2	621	625	PF04695	0.482
LIG_Pex14_2	720	724	PF04695	0.531
LIG_SH2_CRK	532	536	PF00017	0.436
LIG_SH2_CRK	663	667	PF00017	0.475
LIG_SH2_CRK	76	80	PF00017	0.468
LIG_SH2_PTP2	514	517	PF00017	0.470
LIG_SH2_SRC	110	113	PF00017	0.336
LIG_SH2_SRC	514	517	PF00017	0.502
LIG_SH2_STAP1	110	114	PF00017	0.304
LIG_SH2_STAP1	130	134	PF00017	0.420
LIG_SH2_STAP1	235	239	PF00017	0.423
LIG_SH2_STAP1	657	661	PF00017	0.404
LIG_SH2_STAT3	273	276	PF00017	0.445
LIG_SH2_STAT3	589	592	PF00017	0.436
LIG_SH2_STAT3	723	726	PF00017	0.575
LIG_SH2_STAT5	181	184	PF00017	0.298
LIG_SH2_STAT5	186	189	PF00017	0.292
LIG_SH2_STAT5	199	202	PF00017	0.310
LIG_SH2_STAT5	235	238	PF00017	0.500
LIG_SH2_STAT5	273	276	PF00017	0.445
LIG_SH2_STAT5	430	433	PF00017	0.553
LIG_SH2_STAT5	511	514	PF00017	0.477
LIG_SH2_STAT5	52	55	PF00017	0.538
LIG_SH2_STAT5	657	660	PF00017	0.436
LIG_SH2_STAT5	723	726	PF00017	0.591
LIG_SH3_3	146	152	PF00018	0.493
LIG_SH3_3	383	389	PF00018	0.586
LIG_SH3_3	54	60	PF00018	0.530
LIG_SH3_3	730	736	PF00018	0.703
LIG_SUMO_SIM_par_1	502	508	PF11976	0.451
LIG_SUMO_SIM_par_1	519	525	PF11976	0.534
LIG_SUMO_SIM_par_1	594	600	PF11976	0.449
LIG_SUMO_SIM_par_1	694	700	PF11976	0.454
LIG_TRAF2_1	228	231	PF00917	0.578
LIG_TRAF2_1	82	85	PF00917	0.411
LIG_TYR_ITIM	352	357	PF00017	0.531
LIG_TYR_ITIM	665	670	PF00017	0.531
LIG_UBA3_1	249	256	PF00899	0.306
LIG_UBA3_1	520	527	PF00899	0.482
LIG_WRC_WIRS_1	154	159	PF05994	0.351
LIG_WRC_WIRS_1	8	13	PF05994	0.377
MOD_CK1_1	207	213	PF00069	0.533
MOD_CK1_1	219	225	PF00069	0.604
MOD_CK1_1	381	387	PF00069	0.536
MOD_CK1_1	525	531	PF00069	0.462
MOD_CK1_1	554	560	PF00069	0.520
MOD_CK1_1	597	603	PF00069	0.531
MOD_CK1_1	630	636	PF00069	0.408
MOD_CK1_1	648	654	PF00069	0.517
MOD_CK1_1	7	13	PF00069	0.472
MOD_CK1_1	743	749	PF00069	0.710
MOD_CK1_1	75	81	PF00069	0.487
MOD_CK1_1	750	756	PF00069	0.685
MOD_CK1_1	762	768	PF00069	0.581
MOD_CK1_1	778	784	PF00069	0.695
MOD_CK2_1	132	138	PF00069	0.413
MOD_CK2_1	278	284	PF00069	0.521
MOD_CK2_1	673	679	PF00069	0.507
MOD_CK2_1	682	688	PF00069	0.471
MOD_GlcNHglycan	12	15	PF01048	0.535
MOD_GlcNHglycan	120	123	PF01048	0.453
MOD_GlcNHglycan	218	221	PF01048	0.739
MOD_GlcNHglycan	24	27	PF01048	0.704
MOD_GlcNHglycan	373	376	PF01048	0.265
MOD_GlcNHglycan	383	386	PF01048	0.267
MOD_GlcNHglycan	427	430	PF01048	0.373
MOD_GlcNHglycan	44	47	PF01048	0.463
MOD_GlcNHglycan	566	569	PF01048	0.267
MOD_GlcNHglycan	684	687	PF01048	0.235
MOD_GlcNHglycan	740	743	PF01048	0.654
MOD_GlcNHglycan	765	768	PF01048	0.692
MOD_GlcNHglycan	781	784	PF01048	0.692
MOD_GSK3_1	10	17	PF00069	0.523
MOD_GSK3_1	126	133	PF00069	0.448
MOD_GSK3_1	207	214	PF00069	0.652
MOD_GSK3_1	478	485	PF00069	0.383
MOD_GSK3_1	489	496	PF00069	0.386
MOD_GSK3_1	606	613	PF00069	0.508
MOD_GSK3_1	648	655	PF00069	0.524
MOD_GSK3_1	736	743	PF00069	0.598
MOD_GSK3_1	750	757	PF00069	0.634
MOD_GSK3_1	759	766	PF00069	0.717
MOD_GSK3_1	774	781	PF00069	0.765
MOD_LATS_1	752	758	PF00433	0.567
MOD_N-GLC_1	673	678	PF02516	0.297
MOD_N-GLC_2	650	652	PF02516	0.331
MOD_NEK2_1	132	137	PF00069	0.472
MOD_NEK2_1	153	158	PF00069	0.389
MOD_NEK2_1	171	176	PF00069	0.229
MOD_NEK2_1	179	184	PF00069	0.275
MOD_NEK2_1	441	446	PF00069	0.460
MOD_NEK2_1	448	453	PF00069	0.471
MOD_NEK2_1	522	527	PF00069	0.467
MOD_NEK2_1	549	554	PF00069	0.563
MOD_NEK2_1	582	587	PF00069	0.465
MOD_NEK2_1	607	612	PF00069	0.493
MOD_NEK2_1	72	77	PF00069	0.467
MOD_NEK2_1	747	752	PF00069	0.667
MOD_NEK2_2	652	657	PF00069	0.468
MOD_OFUCOSY	646	652	PF10250	0.317
MOD_PIKK_1	14	20	PF00454	0.425
MOD_PIKK_1	278	284	PF00454	0.472
MOD_PIKK_1	522	528	PF00454	0.470
MOD_PIKK_1	722	728	PF00454	0.617
MOD_PIKK_1	80	86	PF00454	0.412
MOD_PK_1	462	468	PF00069	0.381
MOD_PKA_2	262	268	PF00069	0.386
MOD_PKA_2	418	424	PF00069	0.517
MOD_PKA_2	432	438	PF00069	0.446
MOD_PKA_2	448	454	PF00069	0.439
MOD_PKA_2	482	488	PF00069	0.482
MOD_PKA_2	542	548	PF00069	0.449
MOD_PKA_2	564	570	PF00069	0.499
MOD_PKA_2	680	686	PF00069	0.556
MOD_PKA_2	747	753	PF00069	0.710
MOD_PKA_2	762	768	PF00069	0.649
MOD_PKA_2	778	784	PF00069	0.729
MOD_PKA_2	80	86	PF00069	0.547
MOD_Plk_1	583	589	PF00069	0.482
MOD_Plk_1	630	636	PF00069	0.531
MOD_Plk_4	153	159	PF00069	0.444
MOD_Plk_4	171	177	PF00069	0.250
MOD_Plk_4	289	295	PF00069	0.413
MOD_Plk_4	315	321	PF00069	0.385
MOD_Plk_4	4	10	PF00069	0.368
MOD_Plk_4	630	636	PF00069	0.457
MOD_Plk_4	638	644	PF00069	0.436
MOD_Plk_4	652	658	PF00069	0.490
MOD_Plk_4	75	81	PF00069	0.404
MOD_ProDKin_1	116	122	PF00069	0.449
MOD_ProDKin_1	19	25	PF00069	0.578
MOD_ProDKin_1	241	247	PF00069	0.358
MOD_ProDKin_1	378	384	PF00069	0.535
MOD_ProDKin_1	673	679	PF00069	0.544
MOD_SUMO_for_1	601	604	PF00179	0.517
MOD_SUMO_rev_2	2	7	PF00179	0.487
MOD_SUMO_rev_2	406	411	PF00179	0.365
TRG_DiLeu_BaEn_1	246	251	PF01217	0.358
TRG_DiLeu_BaEn_1	257	262	PF01217	0.407
TRG_DiLeu_BaLyEn_6	149	154	PF01217	0.465
TRG_DiLeu_BaLyEn_6	166	171	PF01217	0.454
TRG_DiLeu_BaLyEn_6	383	388	PF01217	0.435
TRG_ENDOCYTIC_2	354	357	PF00928	0.531
TRG_ENDOCYTIC_2	454	457	PF00928	0.449
TRG_ENDOCYTIC_2	532	535	PF00928	0.436
TRG_ENDOCYTIC_2	590	593	PF00928	0.505
TRG_ENDOCYTIC_2	667	670	PF00928	0.499
TRG_ENDOCYTIC_2	76	79	PF00928	0.465
TRG_ER_diArg_1	167	169	PF00400	0.494
TRG_ER_diArg_1	258	260	PF00400	0.368
TRG_ER_diArg_1	49	51	PF00400	0.454
TRG_ER_diArg_1	532	534	PF00400	0.391
TRG_ER_diArg_1	570	572	PF00400	0.449
TRG_ER_diArg_1	728	731	PF00400	0.627
TRG_Pf-PMV_PEXEL_1	168	173	PF00026	0.357
TRG_Pf-PMV_PEXEL_1	259	264	PF00026	0.410
TRG_Pf-PMV_PEXEL_1	553	558	PF00026	0.311

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IC04	Leptomonas seymouri	64%	98%
A0A1X0P204	Trypanosomatidae	44%	100%
A0A3Q8IEU3	Leishmania donovani	89%	100%
A0A422P4W6	Trypanosoma rangeli	43%	100%
A4HI49	Leishmania braziliensis	82%	100%
A4I5C5	Leishmania infantum	89%	100%
C9ZQQ8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	100%
Q4Q7K9	Leishmania major	91%	100%
V5B937	Trypanosoma cruzi	44%	100%