Putative co-chaperone, GrpE

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

heat shock protein grpe

Species:

Leishmania mexicana

UniProt:

E9B0J9_LEIMU

TriTrypDb:

LmxM.29.0735

Length:

243

Annotations

LeishMANIAdb annotations

Homologous to other Eukaryotic mitochondrial proteins. Likely non-TM.

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 6
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0001405	PAM complex, Tim23 associated import motor	4	1
GO:0032991	protein-containing complex	1	1
GO:0098796	membrane protein complex	2	1
GO:0098798	mitochondrial protein-containing complex	2	1
GO:0098800	inner mitochondrial membrane protein complex	3	1
GO:0016020	membrane	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9B0J9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B0J9

Function

Biological processes
Term	Name	Level	Count
GO:0006457	protein folding	2	7
GO:0009987	cellular process	1	7
GO:0006810	transport	3	1
GO:0006839	mitochondrial transport	4	1
GO:0006886	intracellular protein transport	4	1
GO:0008104	protein localization	4	1
GO:0015031	protein transport	4	1
GO:0030150	protein import into mitochondrial matrix	4	1
GO:0033036	macromolecule localization	2	1
GO:0033365	protein localization to organelle	5	1
GO:0044743	protein transmembrane import into intracellular organelle	4	1
GO:0045184	establishment of protein localization	3	1
GO:0046907	intracellular transport	3	1
GO:0051179	localization	1	1
GO:0051234	establishment of localization	2	1
GO:0051641	cellular localization	2	1
GO:0051649	establishment of localization in cell	3	1
GO:0055085	transmembrane transport	2	1
GO:0065002	intracellular protein transmembrane transport	4	1
GO:0070585	protein localization to mitochondrion	6	1
GO:0070727	cellular macromolecule localization	3	1
GO:0071702	organic substance transport	4	1
GO:0071705	nitrogen compound transport	4	1
GO:0071806	protein transmembrane transport	3	1
GO:0072594	establishment of protein localization to organelle	4	1
GO:0072655	establishment of protein localization to mitochondrion	5	1
GO:1990542	mitochondrial transmembrane transport	3	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	7
GO:0000774	adenyl-nucleotide exchange factor activity	3	7
GO:0005488	binding	1	7
GO:0005515	protein binding	2	7
GO:0017076	purine nucleotide binding	4	7
GO:0030554	adenyl nucleotide binding	5	7
GO:0036094	small molecule binding	2	7
GO:0042802	identical protein binding	3	7
GO:0042803	protein homodimerization activity	4	7
GO:0046983	protein dimerization activity	3	7
GO:0051087	protein-folding chaperone binding	3	7
GO:0060590	ATPase regulator activity	2	7
GO:0097159	organic cyclic compound binding	2	7
GO:0098772	molecular function regulator activity	1	7
GO:1901265	nucleoside phosphate binding	3	7
GO:1901363	heterocyclic compound binding	2	7
GO:0051082	unfolded protein binding	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	110	112	PF00675	0.244
CLV_NRD_NRD_1	144	146	PF00675	0.213
CLV_NRD_NRD_1	233	235	PF00675	0.247
CLV_NRD_NRD_1	41	43	PF00675	0.512
CLV_NRD_NRD_1	58	60	PF00675	0.572
CLV_PCSK_KEX2_1	180	182	PF00082	0.280
CLV_PCSK_KEX2_1	233	235	PF00082	0.390
CLV_PCSK_KEX2_1	41	43	PF00082	0.509
CLV_PCSK_KEX2_1	90	92	PF00082	0.255
CLV_PCSK_PC1ET2_1	180	182	PF00082	0.280
CLV_PCSK_PC1ET2_1	90	92	PF00082	0.255
CLV_PCSK_SKI1_1	131	135	PF00082	0.211
CLV_PCSK_SKI1_1	141	145	PF00082	0.229
CLV_PCSK_SKI1_1	177	181	PF00082	0.286
CLV_PCSK_SKI1_1	202	206	PF00082	0.286
CLV_Separin_Metazoa	80	84	PF03568	0.455
DOC_CKS1_1	207	212	PF01111	0.471
DOC_CKS1_1	29	34	PF01111	0.408
DOC_MAPK_gen_1	233	239	PF00069	0.480
DOC_MAPK_RevD_3	45	60	PF00069	0.500
DOC_PP2B_LxvP_1	16	19	PF13499	0.437
DOC_PP4_FxxP_1	204	207	PF00568	0.486
DOC_USP7_MATH_1	103	107	PF00917	0.501
DOC_USP7_MATH_1	125	129	PF00917	0.533
DOC_WW_Pin1_4	206	211	PF00397	0.471
DOC_WW_Pin1_4	28	33	PF00397	0.469
DOC_WW_Pin1_4	45	50	PF00397	0.453
LIG_14-3-3_CanoR_1	181	191	PF00244	0.486
LIG_Actin_WH2_2	126	143	PF00022	0.380
LIG_Actin_WH2_2	199	215	PF00022	0.411
LIG_FHA_1	142	148	PF00498	0.444
LIG_FHA_1	169	175	PF00498	0.455
LIG_FHA_1	207	213	PF00498	0.444
LIG_FHA_1	46	52	PF00498	0.445
LIG_FHA_1	5	11	PF00498	0.434
LIG_FHA_1	65	71	PF00498	0.640
LIG_FHA_2	111	117	PF00498	0.423
LIG_FHA_2	192	198	PF00498	0.423
LIG_LIR_Apic_2	201	207	PF02991	0.444
LIG_LIR_Nem_3	24	28	PF02991	0.418
LIG_SH2_NCK_1	102	106	PF00017	0.486
LIG_SH2_STAT5	150	153	PF00017	0.429
LIG_SH2_STAT5	34	37	PF00017	0.424
LIG_SH3_3	204	210	PF00018	0.464
LIG_SUMO_SIM_par_1	132	138	PF11976	0.444
LIG_SxIP_EBH_1	150	159	PF03271	0.455
LIG_TRAF2_1	113	116	PF00917	0.486
LIG_Vh1_VBS_1	133	151	PF01044	0.413
LIG_WRC_WIRS_1	126	131	PF05994	0.486
LIG_WRC_WIRS_1	22	27	PF05994	0.413
MOD_CDK_SPK_2	28	33	PF00069	0.464
MOD_CDK_SPxxK_3	206	213	PF00069	0.429
MOD_CK1_1	160	166	PF00069	0.404
MOD_CK1_1	208	214	PF00069	0.564
MOD_CK2_1	110	116	PF00069	0.423
MOD_CK2_1	191	197	PF00069	0.423
MOD_CK2_1	208	214	PF00069	0.453
MOD_CK2_1	68	74	PF00069	0.482
MOD_GSK3_1	111	118	PF00069	0.455
MOD_GSK3_1	148	155	PF00069	0.457
MOD_GSK3_1	182	189	PF00069	0.444
MOD_GSK3_1	202	209	PF00069	0.418
MOD_GSK3_1	60	67	PF00069	0.598
MOD_N-GLC_1	227	232	PF02516	0.244
MOD_NEK2_1	12	17	PF00069	0.435
MOD_NEK2_1	227	232	PF00069	0.476
MOD_NEK2_1	50	55	PF00069	0.673
MOD_NEK2_1	86	91	PF00069	0.486
MOD_NEK2_2	21	26	PF00069	0.411
MOD_PIKK_1	111	117	PF00454	0.486
MOD_PIKK_1	148	154	PF00454	0.496
MOD_PIKK_1	182	188	PF00454	0.486
MOD_PK_1	213	219	PF00069	0.429
MOD_PKA_1	111	117	PF00069	0.486
MOD_PKA_2	110	116	PF00069	0.423
MOD_Plk_1	160	166	PF00069	0.453
MOD_Plk_4	152	158	PF00069	0.478
MOD_Plk_4	160	166	PF00069	0.440
MOD_Plk_4	21	27	PF00069	0.454
MOD_Plk_4	227	233	PF00069	0.444
MOD_ProDKin_1	206	212	PF00069	0.471
MOD_ProDKin_1	28	34	PF00069	0.468
MOD_ProDKin_1	45	51	PF00069	0.449
MOD_SUMO_for_1	158	161	PF00179	0.455
MOD_SUMO_rev_2	196	204	PF00179	0.486
TRG_DiLeu_BaLyEn_6	46	51	PF01217	0.550
TRG_ENDOCYTIC_2	79	82	PF00928	0.444
TRG_ER_diArg_1	232	234	PF00400	0.486
TRG_ER_diArg_1	40	42	PF00400	0.513
TRG_Pf-PMV_PEXEL_1	33	37	PF00026	0.452
TRG_Pf-PMV_PEXEL_1	91	95	PF00026	0.230

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HZM2	Leptomonas seymouri	48%	100%
A0A0N1ILR2	Leptomonas seymouri	31%	100%
A0A0S4JEA6	Bodo saltans	32%	100%
A0A1X0P2B4	Trypanosomatidae	31%	100%
A0A3Q8IEN8	Leishmania donovani	88%	95%
A0A3Q8IJ81	Leishmania donovani	32%	100%
A0A422P4T2	Trypanosoma rangeli	25%	100%
A0KZB0	Shewanella sp. (strain ANA-3)	23%	100%
A1AXV2	Ruthia magnifica subsp. Calyptogena magnifica	26%	100%
A1RLV4	Shewanella sp. (strain W3-18-1)	26%	100%
A1WAR5	Acidovorax sp. (strain JS42)	26%	100%
A3D2B1	Shewanella baltica (strain OS155 / ATCC BAA-1091)	27%	100%
A4HI25	Leishmania braziliensis	32%	100%
A4HI26	Leishmania braziliensis	72%	100%
A4I5A2	Leishmania infantum	32%	100%
A4I5A3	Leishmania infantum	93%	100%
A4VPQ6	Pseudomonas stutzeri (strain A1501)	31%	100%
A4XYF7	Pseudomonas mendocina (strain ymp)	28%	100%
A4Y4W9	Shewanella putrefaciens (strain CN-32 / ATCC BAA-453)	26%	100%
A5F369	Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)	25%	100%
A5IDK9	Legionella pneumophila (strain Corby)	25%	100%
A5V5Q2	Rhizorhabdus wittichii (strain DSM 6014 / CCUG 31198 / JCM 15750 / NBRC 105917 / EY 4224 / RW1)	27%	100%
A5VNA6	Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512)	35%	100%
A6VCL9	Pseudomonas aeruginosa (strain PA7)	28%	100%
A6WL03	Shewanella baltica (strain OS185)	27%	100%
A8FYL1	Shewanella sediminis (strain HAW-EB3)	26%	100%
A8H6X2	Shewanella pealeana (strain ATCC 700345 / ANG-SQ1)	25%	100%
A9KTL2	Shewanella baltica (strain OS195)	27%	100%
A9M7B6	Brucella canis (strain ATCC 23365 / NCTC 10854)	35%	100%
B0CJ30	Brucella suis (strain ATCC 23445 / NCTC 10510)	35%	100%
B0R3H6	Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)	25%	100%
B0TQ37	Shewanella halifaxensis (strain HAW-EB4)	26%	100%
B1KQY9	Shewanella woodyi (strain ATCC 51908 / MS32)	26%	100%
B2FMY4	Stenotrophomonas maltophilia (strain K279a)	30%	100%
B3PZA4	Rhizobium etli (strain CIAT 652)	32%	100%
B4F059	Proteus mirabilis (strain HI4320)	29%	100%
B4S9D1	Prosthecochloris aestuarii (strain DSM 271 / SK 413)	27%	100%
B5FA13	Aliivibrio fischeri (strain MJ11)	26%	100%
B6EKA3	Aliivibrio salmonicida (strain LFI1238)	27%	100%
B6JCI1	Afipia carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5)	29%	100%
B7V1H4	Pseudomonas aeruginosa (strain LESB58)	30%	100%
B7VJW7	Vibrio atlanticus (strain LGP32)	26%	100%
B8CS27	Shewanella piezotolerans (strain WP3 / JCM 13877)	26%	100%
B8EAU9	Shewanella baltica (strain OS223)	27%	100%
B8GNX0	Thioalkalivibrio sulfidiphilus (strain HL-EbGR7)	28%	100%
B9MDJ6	Acidovorax ebreus (strain TPSY)	26%	100%
C1DFM4	Azotobacter vinelandii (strain DJ / ATCC BAA-1303)	30%	100%
C3K276	Pseudomonas fluorescens (strain SBW25)	27%	100%
C3LTA4	Vibrio cholerae serotype O1 (strain M66-2)	25%	100%
C3PP76	Rickettsia africae (strain ESF-5)	23%	100%
C4K2U3	Rickettsia peacockii (strain Rustic)	23%	100%
C5BQ34	Teredinibacter turnerae (strain ATCC 39867 / T7901)	29%	100%
C9ZQN6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	32%	100%
E9B0J8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
O30862	Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)	25%	100%
O32481	Legionella pneumophila	25%	100%
O43047	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	30%	100%
O88396	Mus musculus	27%	100%
P38523	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	24%	100%
P43732	Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)	27%	100%
P48604	Drosophila melanogaster	28%	100%
P63187	Agrobacterium fabrum (strain C58 / ATCC 33970)	32%	100%
P63188	Rhizobium radiobacter	32%	100%
Q02FR0	Pseudomonas aeruginosa (strain UCBPP-PA14)	29%	100%
Q07ZD3	Shewanella frigidimarina (strain NCIMB 400)	29%	100%
Q0BX02	Hyphomonas neptunium (strain ATCC 15444)	28%	100%
Q0HGL4	Shewanella sp. (strain MR-4)	23%	100%
Q0HSW5	Shewanella sp. (strain MR-7)	23%	100%
Q0P5N5	Bos taurus	28%	100%
Q12L25	Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013)	29%	100%
Q18421	Caenorhabditis elegans	25%	100%
Q1MPH5	Lawsonia intracellularis (strain PHE/MN1-00)	26%	100%
Q1QSW9	Chromohalobacter salexigens (strain ATCC BAA-138 / DSM 3043 / CIP 106854 / NCIMB 13768 / 1H11)	28%	100%
Q21H35	Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024)	27%	100%
Q2G6M5	Novosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CCUG 56034 / CIP 105152 / NBRC 16084 / F199)	26%	100%
Q2GHU0	Ehrlichia chaffeensis (strain ATCC CRL-10679 / Arkansas)	26%	100%
Q2KD99	Rhizobium etli (strain CFN 42 / ATCC 51251)	31%	100%
Q2NAJ5	Erythrobacter litoralis (strain HTCC2594)	26%	100%
Q3IYI4	Cereibacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / CCUG 31486 / LMG 2827 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.)	29%	100%
Q3KIA1	Pseudomonas fluorescens (strain Pf0-1)	26%	100%
Q3SZC1	Bos taurus	28%	100%
Q3YSZ3	Ehrlichia canis (strain Jake)	26%	100%
Q48E61	Pseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6)	26%	100%
Q4KIH2	Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)	27%	100%
Q4Q7N3	Leishmania major	88%	100%
Q4Q7N4	Leishmania major	32%	100%
Q4ZNP6	Pseudomonas syringae pv. syringae (strain B728a)	26%	100%
Q5E3A5	Aliivibrio fischeri (strain ATCC 700601 / ES114)	26%	100%
Q5RA81	Pongo abelii	28%	100%
Q5WV14	Legionella pneumophila (strain Lens)	25%	100%
Q5X3M6	Legionella pneumophila (strain Paris)	24%	100%
Q5ZTY2	Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513)	25%	100%
Q68WA8	Rickettsia typhi (strain ATCC VR-144 / Wilmington)	22%	100%
Q6BTP9	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	30%	100%
Q6CRQ1	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	28%	100%
Q6FPH2	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	26%	100%
Q6G563	Bartonella henselae (strain ATCC 49882 / DSM 28221 / Houston 1)	31%	100%
Q6IT00	Vibrio harveyi	26%	100%
Q75C01	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	27%	100%
Q79V15	Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110)	31%	100%
Q7N1U7	Photorhabdus laumondii subsp. laumondii (strain DSM 15139 / CIP 105565 / TT01)	30%	100%
Q7VMB7	Haemophilus ducreyi (strain 35000HP / ATCC 700724)	26%	100%
Q87RX5	Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)	29%	100%
Q87WN9	Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)	26%	100%
Q89AS0	Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp)	25%	100%
Q8EGS0	Shewanella oneidensis (strain MR-1)	23%	100%
Q8G2Y6	Brucella suis biovar 1 (strain 1330)	35%	100%
Q8YEV0	Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)	35%	100%
Q92GZ5	Rickettsia conorii (strain ATCC VR-613 / Malish 7)	23%	100%
Q92SK0	Rhizobium meliloti (strain 1021)	33%	100%
Q98GQ5	Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)	33%	100%
Q9HAV7	Homo sapiens	28%	100%
Q9HRY0	Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)	25%	100%
Q9HV42	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	29%	100%
Q9L7Z3	Vibrio proteolyticus	24%	100%
Q9P5U4	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	29%	100%