Putative spliceosome-associated protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

spliceosome-associated protein, putative

Species:

Leishmania mexicana

UniProt:

E9B0I2_LEIMU

TriTrypDb:

LmxM.29.0570

Length:

473

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	10
GO:0043226	organelle	2	10
GO:0043227	membrane-bounded organelle	3	10
GO:0043229	intracellular organelle	3	10
GO:0043231	intracellular membrane-bounded organelle	4	10
GO:0110165	cellular anatomical entity	1	10
GO:0005681	spliceosomal complex	3	1
GO:0005684	U2-type spliceosomal complex	4	1
GO:0005686	U2 snRNP	5	1
GO:0030532	small nuclear ribonucleoprotein complex	3	1
GO:0032991	protein-containing complex	1	1
GO:0071011	precatalytic spliceosome	4	1
GO:0071013	catalytic step 2 spliceosome	3	1
GO:0097525	spliceosomal snRNP complex	4	1
GO:0120114	Sm-like protein family complex	2	1
GO:0140513	nuclear protein-containing complex	2	1
GO:1902494	catalytic complex	2	1
GO:1990904	ribonucleoprotein complex	2	1

Expansion

Sequence features

E9B0I2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B0I2

Function

Biological processes
Term	Name	Level	Count
GO:0000375	RNA splicing, via transesterification reactions	8	1
GO:0000377	RNA splicing, via transesterification reactions with bulged adenosine as nucleophile	9	1
GO:0000398	mRNA splicing, via spliceosome	8	1
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006396	RNA processing	6	1
GO:0006397	mRNA processing	7	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0008380	RNA splicing	7	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0016071	mRNA metabolic process	6	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0043170	macromolecule metabolic process	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	117	119	PF00675	0.636
CLV_NRD_NRD_1	12	14	PF00675	0.479
CLV_NRD_NRD_1	126	128	PF00675	0.525
CLV_NRD_NRD_1	240	242	PF00675	0.448
CLV_NRD_NRD_1	297	299	PF00675	0.287
CLV_PCSK_KEX2_1	117	119	PF00082	0.639
CLV_PCSK_KEX2_1	12	14	PF00082	0.458
CLV_PCSK_KEX2_1	273	275	PF00082	0.330
CLV_PCSK_KEX2_1	297	299	PF00082	0.287
CLV_PCSK_PC1ET2_1	273	275	PF00082	0.330
CLV_PCSK_SKI1_1	101	105	PF00082	0.695
CLV_PCSK_SKI1_1	186	190	PF00082	0.291
CLV_PCSK_SKI1_1	276	280	PF00082	0.287
CLV_PCSK_SKI1_1	29	33	PF00082	0.591
CLV_Separin_Metazoa	330	334	PF03568	0.543
DEG_APCC_DBOX_1	100	108	PF00400	0.558
DEG_SIAH_1	424	432	PF03145	0.665
DOC_CKS1_1	407	412	PF01111	0.676
DOC_CKS1_1	50	55	PF01111	0.533
DOC_CYCLIN_RxL_1	26	34	PF00134	0.446
DOC_CYCLIN_yCln2_LP_2	311	317	PF00134	0.394
DOC_MAPK_DCC_7	405	413	PF00069	0.546
DOC_MAPK_FxFP_2	339	342	PF00069	0.427
DOC_MAPK_gen_1	241	248	PF00069	0.419
DOC_MAPK_gen_1	273	281	PF00069	0.405
DOC_PP2B_LxvP_1	306	309	PF13499	0.277
DOC_PP2B_LxvP_1	311	314	PF13499	0.259
DOC_PP4_FxxP_1	339	342	PF00568	0.427
DOC_USP7_MATH_1	350	354	PF00917	0.724
DOC_USP7_MATH_1	377	381	PF00917	0.785
DOC_USP7_UBL2_3	259	263	PF12436	0.440
DOC_USP7_UBL2_3	5	9	PF12436	0.547
DOC_WW_Pin1_4	234	239	PF00397	0.411
DOC_WW_Pin1_4	264	269	PF00397	0.495
DOC_WW_Pin1_4	383	388	PF00397	0.634
DOC_WW_Pin1_4	397	402	PF00397	0.651
DOC_WW_Pin1_4	406	411	PF00397	0.529
DOC_WW_Pin1_4	427	432	PF00397	0.583
DOC_WW_Pin1_4	457	462	PF00397	0.605
DOC_WW_Pin1_4	49	54	PF00397	0.613
LIG_14-3-3_CanoR_1	117	124	PF00244	0.652
LIG_14-3-3_CanoR_1	131	135	PF00244	0.382
LIG_14-3-3_CanoR_1	241	245	PF00244	0.502
LIG_Actin_WH2_2	42	59	PF00022	0.526
LIG_BIR_II_1	1	5	PF00653	0.548
LIG_BRCT_BRCA1_1	337	341	PF00533	0.567
LIG_EVH1_1	49	53	PF00568	0.539
LIG_FHA_1	148	154	PF00498	0.350
LIG_FHA_1	173	179	PF00498	0.294
LIG_FHA_1	241	247	PF00498	0.518
LIG_FHA_1	265	271	PF00498	0.347
LIG_FHA_1	443	449	PF00498	0.546
LIG_FHA_2	131	137	PF00498	0.555
LIG_FHA_2	352	358	PF00498	0.566
LIG_FHA_2	366	372	PF00498	0.483
LIG_FHA_2	428	434	PF00498	0.622
LIG_FHA_2	50	56	PF00498	0.614
LIG_LIR_Apic_2	326	331	PF02991	0.418
LIG_LIR_Apic_2	336	342	PF02991	0.444
LIG_LIR_Apic_2	44	50	PF02991	0.572
LIG_LIR_Apic_2	465	470	PF02991	0.575
LIG_LIR_Gen_1	133	142	PF02991	0.422
LIG_LIR_Gen_1	433	439	PF02991	0.553
LIG_LIR_Nem_3	133	137	PF02991	0.432
LIG_LIR_Nem_3	338	344	PF02991	0.435
LIG_LIR_Nem_3	357	363	PF02991	0.374
LIG_LIR_Nem_3	433	437	PF02991	0.557
LIG_LYPXL_yS_3	303	306	PF13949	0.269
LIG_Pex14_2	229	233	PF04695	0.426
LIG_SH2_CRK	47	51	PF00017	0.562
LIG_SH2_NCK_1	47	51	PF00017	0.634
LIG_SH2_PTP2	434	437	PF00017	0.555
LIG_SH2_STAT3	291	294	PF00017	0.269
LIG_SH2_STAT3	418	421	PF00017	0.656
LIG_SH2_STAT5	418	421	PF00017	0.523
LIG_SH2_STAT5	434	437	PF00017	0.555
LIG_SH2_STAT5	450	453	PF00017	0.568
LIG_SH2_STAT5	47	50	PF00017	0.510
LIG_SH3_1	298	304	PF00018	0.287
LIG_SH3_1	47	53	PF00018	0.553
LIG_SH3_2	461	466	PF14604	0.581
LIG_SH3_3	173	179	PF00018	0.269
LIG_SH3_3	298	304	PF00018	0.248
LIG_SH3_3	404	410	PF00018	0.589
LIG_SH3_3	448	454	PF00018	0.506
LIG_SH3_3	455	461	PF00018	0.497
LIG_SH3_3	47	53	PF00018	0.553
LIG_SH3_3	99	105	PF00018	0.574
LIG_TRAF2_1	133	136	PF00917	0.493
LIG_TRAF2_1	369	372	PF00917	0.628
LIG_TRAF2_1	430	433	PF00917	0.559
LIG_TRAF2_1	58	61	PF00917	0.612
LIG_TRFH_1	47	51	PF08558	0.634
LIG_WW_1	300	303	PF00397	0.269
LIG_WW_3	330	334	PF00397	0.410
MOD_CDK_SPxxK_3	234	241	PF00069	0.417
MOD_CK1_1	122	128	PF00069	0.651
MOD_CK1_1	292	298	PF00069	0.330
MOD_CK2_1	1	7	PF00069	0.585
MOD_CK2_1	130	136	PF00069	0.580
MOD_CK2_1	351	357	PF00069	0.549
MOD_CK2_1	365	371	PF00069	0.491
MOD_CK2_1	427	433	PF00069	0.616
MOD_CK2_1	49	55	PF00069	0.595
MOD_Cter_Amidation	115	118	PF01082	0.733
MOD_Cter_Amidation	125	128	PF01082	0.636
MOD_GlcNHglycan	121	124	PF01048	0.695
MOD_GlcNHglycan	381	384	PF01048	0.697
MOD_GSK3_1	118	125	PF00069	0.716
MOD_GSK3_1	228	235	PF00069	0.425
MOD_GSK3_1	379	386	PF00069	0.670
MOD_GSK3_1	438	445	PF00069	0.531
MOD_GSK3_1	45	52	PF00069	0.626
MOD_N-GLC_1	439	444	PF02516	0.671
MOD_NEK2_1	1	6	PF00069	0.640
MOD_NEK2_1	184	189	PF00069	0.356
MOD_NEK2_2	163	168	PF00069	0.311
MOD_NEK2_2	228	233	PF00069	0.436
MOD_PIKK_1	20	26	PF00454	0.542
MOD_PIKK_1	351	357	PF00454	0.574
MOD_PKA_1	117	123	PF00069	0.688
MOD_PKA_1	20	26	PF00069	0.521
MOD_PKA_2	117	123	PF00069	0.591
MOD_PKA_2	130	136	PF00069	0.374
MOD_PKA_2	214	220	PF00069	0.258
MOD_PKA_2	240	246	PF00069	0.557
MOD_Plk_1	335	341	PF00069	0.516
MOD_Plk_1	442	448	PF00069	0.594
MOD_Plk_4	335	341	PF00069	0.456
MOD_ProDKin_1	234	240	PF00069	0.417
MOD_ProDKin_1	264	270	PF00069	0.494
MOD_ProDKin_1	383	389	PF00069	0.631
MOD_ProDKin_1	397	403	PF00069	0.648
MOD_ProDKin_1	406	412	PF00069	0.517
MOD_ProDKin_1	427	433	PF00069	0.587
MOD_ProDKin_1	457	463	PF00069	0.605
MOD_ProDKin_1	49	55	PF00069	0.613
MOD_SUMO_for_1	169	172	PF00179	0.402
MOD_SUMO_rev_2	135	139	PF00179	0.445
MOD_SUMO_rev_2	4	11	PF00179	0.609
TRG_DiLeu_BaEn_1	148	153	PF01217	0.396
TRG_DiLeu_BaEn_2	334	340	PF01217	0.440
TRG_DiLeu_BaEn_3	196	202	PF01217	0.402
TRG_ENDOCYTIC_2	303	306	PF00928	0.269
TRG_ENDOCYTIC_2	434	437	PF00928	0.570
TRG_ENDOCYTIC_2	450	453	PF00928	0.568
TRG_ER_diArg_1	296	298	PF00400	0.287

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P7T1	Leptomonas seymouri	70%	98%
A0A1X0P248	Trypanosomatidae	48%	100%
A0A3R7MML5	Trypanosoma rangeli	49%	100%
A0A3S5H7M4	Leishmania donovani	95%	100%
A4HI08	Leishmania braziliensis	87%	100%
A4I585	Leishmania infantum	95%	100%
C9ZQL4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	49%	99%
Q4Q7P9	Leishmania major	95%	100%
V5B7R6	Trypanosoma cruzi	48%	100%