N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

glycosyltransferase family 28 protein, putative

Species:

Leishmania mexicana

UniProt:

E9B0H8_LEIMU

TriTrypDb:

LmxM.29.0530

Length:

437

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005783	endoplasmic reticulum	5	12
GO:0016020	membrane	2	12
GO:0031090	organelle membrane	3	12
GO:0031965	nuclear membrane	4	12
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0043229	intracellular organelle	3	12
GO:0043231	intracellular membrane-bounded organelle	4	12
GO:0110165	cellular anatomical entity	1	12

Expansion

Sequence features

E9B0H8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B0H8

Function

Biological processes
Term	Name	Level	Count
GO:0006488	dolichol-linked oligosaccharide biosynthetic process	5	12
GO:0006490	oligosaccharide-lipid intermediate biosynthetic process	4	12
GO:0006629	lipid metabolic process	3	12
GO:0008152	metabolic process	1	12
GO:0009058	biosynthetic process	2	12
GO:0009987	cellular process	1	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044255	cellular lipid metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:1901135	carbohydrate derivative metabolic process	3	12
GO:1901137	carbohydrate derivative biosynthetic process	4	12
GO:1901576	organic substance biosynthetic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0004577	N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity	6	9
GO:0008194	UDP-glycosyltransferase activity	4	9
GO:0008375	acetylglucosaminyltransferase activity	5	9
GO:0016740	transferase activity	2	12
GO:0016757	glycosyltransferase activity	3	12
GO:0016758	hexosyltransferase activity	4	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_MEL_PAP_1	269	275	PF00089	0.325
CLV_NRD_NRD_1	211	213	PF00675	0.328
CLV_NRD_NRD_1	94	96	PF00675	0.310
CLV_PCSK_FUR_1	208	212	PF00082	0.362
CLV_PCSK_KEX2_1	208	210	PF00082	0.293
CLV_PCSK_KEX2_1	211	213	PF00082	0.291
CLV_PCSK_KEX2_1	276	278	PF00082	0.313
CLV_PCSK_KEX2_1	94	96	PF00082	0.310
CLV_PCSK_PC1ET2_1	276	278	PF00082	0.327
CLV_PCSK_PC7_1	204	210	PF00082	0.366
CLV_PCSK_PC7_1	272	278	PF00082	0.362
CLV_PCSK_SKI1_1	335	339	PF00082	0.325
CLV_PCSK_SKI1_1	421	425	PF00082	0.371
CLV_PCSK_SKI1_1	54	58	PF00082	0.353
CLV_Separin_Metazoa	51	55	PF03568	0.501
DEG_APCC_DBOX_1	211	219	PF00400	0.611
DEG_APCC_DBOX_1	326	334	PF00400	0.487
DEG_APCC_DBOX_1	395	403	PF00400	0.610
DEG_MDM2_SWIB_1	195	202	PF02201	0.517
DEG_Nend_Nbox_1	1	3	PF02207	0.340
DOC_CYCLIN_yCln2_LP_2	186	192	PF00134	0.562
DOC_MAPK_gen_1	208	218	PF00069	0.510
DOC_MAPK_MEF2A_6	34	43	PF00069	0.579
DOC_PP2B_LxvP_1	186	189	PF13499	0.501
DOC_SPAK_OSR1_1	272	276	PF12202	0.525
DOC_USP7_MATH_1	305	309	PF00917	0.581
DOC_WW_Pin1_4	28	33	PF00397	0.679
LIG_14-3-3_CanoR_1	120	124	PF00244	0.439
LIG_14-3-3_CanoR_1	224	230	PF00244	0.706
LIG_14-3-3_CanoR_1	232	237	PF00244	0.688
LIG_14-3-3_CanoR_1	335	340	PF00244	0.562
LIG_14-3-3_CanoR_1	365	375	PF00244	0.513
LIG_14-3-3_CanoR_1	54	63	PF00244	0.553
LIG_BRCT_BRCA1_1	249	253	PF00533	0.466
LIG_CaM_IQ_9	413	429	PF13499	0.447
LIG_EH1_1	2	10	PF00400	0.281
LIG_FHA_1	111	117	PF00498	0.587
LIG_FHA_1	181	187	PF00498	0.550
LIG_FHA_1	282	288	PF00498	0.509
LIG_FHA_1	336	342	PF00498	0.556
LIG_FHA_2	346	352	PF00498	0.476
LIG_LIR_Gen_1	16	26	PF02991	0.398
LIG_LIR_Gen_1	172	182	PF02991	0.476
LIG_LIR_Gen_1	241	248	PF02991	0.541
LIG_LIR_Gen_1	250	261	PF02991	0.462
LIG_LIR_Nem_3	16	21	PF02991	0.475
LIG_LIR_Nem_3	172	177	PF02991	0.476
LIG_LIR_Nem_3	196	202	PF02991	0.487
LIG_LIR_Nem_3	241	246	PF02991	0.529
LIG_LIR_Nem_3	250	256	PF02991	0.438
LIG_LIR_Nem_3	67	73	PF02991	0.562
LIG_NRBOX	3	9	PF00104	0.399
LIG_Pex14_2	195	199	PF04695	0.508
LIG_PTB_Apo_2	378	385	PF02174	0.553
LIG_SH2_CRK	163	167	PF00017	0.501
LIG_SH2_CRK	243	247	PF00017	0.559
LIG_SH2_CRK	99	103	PF00017	0.528
LIG_SH2_NCK_1	243	247	PF00017	0.562
LIG_SH2_PTP2	24	27	PF00017	0.674
LIG_SH2_STAT5	111	114	PF00017	0.476
LIG_SH2_STAT5	24	27	PF00017	0.674
LIG_SH2_STAT5	279	282	PF00017	0.532
LIG_SH2_STAT5	71	74	PF00017	0.476
LIG_SH2_STAT5	99	102	PF00017	0.502
LIG_SH3_3	141	147	PF00018	0.292
LIG_SH3_3	257	263	PF00018	0.529
LIG_SH3_3	284	290	PF00018	0.525
LIG_SH3_3	294	300	PF00018	0.435
LIG_SH3_3	322	328	PF00018	0.502
LIG_SH3_3	357	363	PF00018	0.504
LIG_Sin3_3	337	344	PF02671	0.476
LIG_SUMO_SIM_par_1	244	251	PF11976	0.476
LIG_SUMO_SIM_par_1	337	342	PF11976	0.547
LIG_SUMO_SIM_par_1	398	404	PF11976	0.627
LIG_TRAF2_1	238	241	PF00917	0.716
LIG_TYR_ITIM	22	27	PF00017	0.674
LIG_WRC_WIRS_1	171	176	PF05994	0.501
MOD_CDC14_SPxK_1	31	34	PF00782	0.698
MOD_CDK_SPxK_1	28	34	PF00069	0.684
MOD_CK1_1	110	116	PF00069	0.551
MOD_CK1_1	178	184	PF00069	0.566
MOD_CK1_1	308	314	PF00069	0.587
MOD_CK1_1	82	88	PF00069	0.581
MOD_CK2_1	345	351	PF00069	0.476
MOD_CK2_1	82	88	PF00069	0.549
MOD_GlcNHglycan	427	430	PF01048	0.454
MOD_GlcNHglycan	44	47	PF01048	0.300
MOD_GlcNHglycan	73	76	PF01048	0.266
MOD_GlcNHglycan	79	82	PF01048	0.251
MOD_GSK3_1	107	114	PF00069	0.477
MOD_GSK3_1	115	122	PF00069	0.492
MOD_GSK3_1	13	20	PF00069	0.430
MOD_GSK3_1	244	251	PF00069	0.480
MOD_GSK3_1	308	315	PF00069	0.542
MOD_GSK3_1	335	342	PF00069	0.549
MOD_GSK3_1	42	49	PF00069	0.498
MOD_GSK3_1	73	80	PF00069	0.488
MOD_NEK2_1	13	18	PF00069	0.411
MOD_NEK2_1	180	185	PF00069	0.485
MOD_NEK2_1	339	344	PF00069	0.479
MOD_NEK2_1	400	405	PF00069	0.603
MOD_NEK2_1	422	427	PF00069	0.650
MOD_PIKK_1	217	223	PF00454	0.560
MOD_PIKK_1	308	314	PF00454	0.525
MOD_PIKK_1	82	88	PF00454	0.581
MOD_PKA_1	211	217	PF00069	0.562
MOD_PKA_2	119	125	PF00069	0.398
MOD_PKA_2	211	217	PF00069	0.551
MOD_PKA_2	223	229	PF00069	0.638
MOD_PKB_1	209	217	PF00069	0.581
MOD_Plk_1	291	297	PF00069	0.565
MOD_Plk_1	400	406	PF00069	0.583
MOD_Plk_4	107	113	PF00069	0.487
MOD_Plk_4	13	19	PF00069	0.396
MOD_Plk_4	211	217	PF00069	0.521
MOD_Plk_4	252	258	PF00069	0.537
MOD_Plk_4	291	297	PF00069	0.558
MOD_Plk_4	335	341	PF00069	0.508
MOD_Plk_4	345	351	PF00069	0.438
MOD_Plk_4	59	65	PF00069	0.538
MOD_ProDKin_1	28	34	PF00069	0.684
MOD_SUMO_for_1	434	437	PF00179	0.654
TRG_DiLeu_BaEn_1	241	246	PF01217	0.555
TRG_DiLeu_BaEn_1	418	423	PF01217	0.627
TRG_DiLeu_BaLyEn_6	182	187	PF01217	0.453
TRG_DiLeu_LyEn_5	395	400	PF01217	0.642
TRG_ENDOCYTIC_2	24	27	PF00928	0.674
TRG_ENDOCYTIC_2	243	246	PF00928	0.585
TRG_ER_diArg_1	102	105	PF00400	0.476
TRG_ER_diArg_1	208	211	PF00400	0.521
TRG_ER_diArg_1	269	272	PF00400	0.373
TRG_ER_diArg_1	94	96	PF00400	0.508
TRG_Pf-PMV_PEXEL_1	234	239	PF00026	0.546
TRG_Pf-PMV_PEXEL_1	365	370	PF00026	0.282
TRG_Pf-PMV_PEXEL_1	54	58	PF00026	0.307

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I7X1	Leptomonas seymouri	62%	100%
A0A0S4IKP3	Bodo saltans	41%	100%
A0A1X0P2G9	Trypanosomatidae	49%	100%
A0A3Q8IBT8	Leishmania donovani	96%	100%
A0A422NIG8	Trypanosoma rangeli	49%	100%
A4HI04	Leishmania braziliensis	84%	100%
A4I581	Leishmania infantum	96%	100%
C9ZQL0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	96%
Q4Q7Q3	Leishmania major	95%	100%
V5BC77	Trypanosoma cruzi	49%	100%