ATP-dependent DNA helicase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

PIF1 helicase-like protein, putative

Species:

Leishmania mexicana

UniProt:

E9B075_LEIMU

TriTrypDb:

LmxM.28.2530

Length:

935

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	11
GO:0110165	cellular anatomical entity	1	11
GO:0005657	replication fork	2	1

Expansion

Sequence features

E9B075

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B075

Function

Biological processes
Term	Name	Level	Count
GO:0000723	telomere maintenance	5	11
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006259	DNA metabolic process	4	11
GO:0006281	DNA repair	5	11
GO:0006310	DNA recombination	5	11
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0006950	response to stress	2	11
GO:0006974	DNA damage response	4	11
GO:0006996	organelle organization	4	11
GO:0008152	metabolic process	1	11
GO:0009987	cellular process	1	11
GO:0016043	cellular component organization	3	11
GO:0032200	telomere organization	6	11
GO:0033554	cellular response to stress	3	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0043170	macromolecule metabolic process	3	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044260	obsolete cellular macromolecule metabolic process	3	11
GO:0046483	heterocycle metabolic process	3	11
GO:0050896	response to stimulus	1	11
GO:0051276	chromosome organization	5	11
GO:0051716	cellular response to stimulus	2	11
GO:0071704	organic substance metabolic process	2	11
GO:0071840	cellular component organization or biogenesis	2	11
GO:0090304	nucleic acid metabolic process	4	11
GO:1901360	organic cyclic compound metabolic process	3	11
GO:0006260	DNA replication	5	1
GO:0032392	DNA geometric change	7	1
GO:0032508	DNA duplex unwinding	8	1
GO:0071103	DNA conformation change	6	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0003678	DNA helicase activity	3	11
GO:0003824	catalytic activity	1	11
GO:0004386	helicase activity	2	11
GO:0005488	binding	1	11
GO:0005524	ATP binding	5	11
GO:0008094	ATP-dependent activity, acting on DNA	2	11
GO:0016462	pyrophosphatase activity	5	11
GO:0016787	hydrolase activity	2	11
GO:0016817	hydrolase activity, acting on acid anhydrides	3	11
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	11
GO:0016887	ATP hydrolysis activity	7	11
GO:0017076	purine nucleotide binding	4	11
GO:0017111	ribonucleoside triphosphate phosphatase activity	6	11
GO:0030554	adenyl nucleotide binding	5	11
GO:0032553	ribonucleotide binding	3	11
GO:0032555	purine ribonucleotide binding	4	11
GO:0032559	adenyl ribonucleotide binding	5	11
GO:0035639	purine ribonucleoside triphosphate binding	4	11
GO:0036094	small molecule binding	2	11
GO:0043167	ion binding	2	11
GO:0043168	anion binding	3	11
GO:0097159	organic cyclic compound binding	2	11
GO:0097367	carbohydrate derivative binding	2	11
GO:0140097	catalytic activity, acting on DNA	3	11
GO:0140640	catalytic activity, acting on a nucleic acid	2	11
GO:0140657	ATP-dependent activity	1	11
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11
GO:0000287	magnesium ion binding	5	1
GO:0043169	cation binding	3	1
GO:0046872	metal ion binding	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	377	381	PF00656	0.335
CLV_C14_Caspase3-7	610	614	PF00656	0.750
CLV_C14_Caspase3-7	620	624	PF00656	0.786
CLV_NRD_NRD_1	157	159	PF00675	0.668
CLV_NRD_NRD_1	226	228	PF00675	0.564
CLV_NRD_NRD_1	457	459	PF00675	0.448
CLV_NRD_NRD_1	487	489	PF00675	0.451
CLV_NRD_NRD_1	562	564	PF00675	0.759
CLV_NRD_NRD_1	659	661	PF00675	0.719
CLV_NRD_NRD_1	672	674	PF00675	0.611
CLV_NRD_NRD_1	772	774	PF00675	0.496
CLV_NRD_NRD_1	873	875	PF00675	0.532
CLV_NRD_NRD_1	892	894	PF00675	0.361
CLV_PCSK_FUR_1	670	674	PF00082	0.713
CLV_PCSK_KEX2_1	156	158	PF00082	0.695
CLV_PCSK_KEX2_1	226	228	PF00082	0.607
CLV_PCSK_KEX2_1	457	459	PF00082	0.509
CLV_PCSK_KEX2_1	486	488	PF00082	0.474
CLV_PCSK_KEX2_1	669	671	PF00082	0.714
CLV_PCSK_KEX2_1	672	674	PF00082	0.657
CLV_PCSK_KEX2_1	772	774	PF00082	0.496
CLV_PCSK_KEX2_1	892	894	PF00082	0.490
CLV_PCSK_PC1ET2_1	669	671	PF00082	0.751
CLV_PCSK_PC7_1	152	158	PF00082	0.631
CLV_PCSK_PC7_1	222	228	PF00082	0.608
CLV_PCSK_SKI1_1	115	119	PF00082	0.445
CLV_PCSK_SKI1_1	167	171	PF00082	0.679
CLV_PCSK_SKI1_1	222	226	PF00082	0.626
CLV_PCSK_SKI1_1	309	313	PF00082	0.311
CLV_PCSK_SKI1_1	39	43	PF00082	0.563
CLV_PCSK_SKI1_1	436	440	PF00082	0.354
CLV_PCSK_SKI1_1	504	508	PF00082	0.317
CLV_PCSK_SKI1_1	594	598	PF00082	0.473
CLV_PCSK_SKI1_1	672	676	PF00082	0.638
CLV_PCSK_SKI1_1	779	783	PF00082	0.564
CLV_PCSK_SKI1_1	893	897	PF00082	0.481
DEG_APCC_DBOX_1	542	550	PF00400	0.522
DEG_COP1_1	565	575	PF00400	0.442
DEG_Nend_UBRbox_2	1	3	PF02207	0.680
DEG_ODPH_VHL_1	730	743	PF01847	0.431
DEG_SCF_FBW7_2	78	85	PF00400	0.479
DEG_SPOP_SBC_1	107	111	PF00917	0.558
DOC_CKS1_1	269	274	PF01111	0.649
DOC_CKS1_1	437	442	PF01111	0.408
DOC_CYCLIN_RxL_1	542	553	PF00134	0.328
DOC_CYCLIN_yCln2_LP_2	269	275	PF00134	0.646
DOC_CYCLIN_yCln2_LP_2	28	34	PF00134	0.476
DOC_CYCLIN_yCln2_LP_2	437	443	PF00134	0.408
DOC_CYCLIN_yCln2_LP_2	469	475	PF00134	0.628
DOC_CYCLIN_yCln2_LP_2	828	834	PF00134	0.517
DOC_MAPK_DCC_7	789	799	PF00069	0.508
DOC_MAPK_gen_1	25	34	PF00069	0.622
DOC_MAPK_gen_1	660	666	PF00069	0.666
DOC_MAPK_gen_1	734	743	PF00069	0.379
DOC_MAPK_gen_1	772	782	PF00069	0.559
DOC_MAPK_gen_1	872	881	PF00069	0.395
DOC_MAPK_gen_1	892	898	PF00069	0.222
DOC_MAPK_MEF2A_6	25	32	PF00069	0.471
DOC_MAPK_MEF2A_6	287	294	PF00069	0.252
DOC_MAPK_MEF2A_6	399	408	PF00069	0.314
DOC_MAPK_MEF2A_6	430	437	PF00069	0.336
DOC_MAPK_MEF2A_6	773	782	PF00069	0.594
DOC_MAPK_RevD_3	879	893	PF00069	0.445
DOC_PP1_RVXF_1	777	783	PF00149	0.541
DOC_PP2B_LxvP_1	28	31	PF13499	0.601
DOC_PP2B_LxvP_1	828	831	PF13499	0.519
DOC_PP4_FxxP_1	21	24	PF00568	0.632
DOC_PP4_FxxP_1	34	37	PF00568	0.592
DOC_PP4_FxxP_1	349	352	PF00568	0.335
DOC_PP4_FxxP_1	68	71	PF00568	0.487
DOC_PP4_FxxP_1	762	765	PF00568	0.415
DOC_PP4_FxxP_1	98	101	PF00568	0.448
DOC_USP7_MATH_1	176	180	PF00917	0.519
DOC_USP7_MATH_1	188	192	PF00917	0.793
DOC_USP7_MATH_1	193	197	PF00917	0.762
DOC_USP7_MATH_1	423	427	PF00917	0.406
DOC_USP7_MATH_1	571	575	PF00917	0.667
DOC_USP7_MATH_1	765	769	PF00917	0.556
DOC_USP7_MATH_1	905	909	PF00917	0.629
DOC_USP7_MATH_1	917	921	PF00917	0.536
DOC_WW_Pin1_4	132	137	PF00397	0.582
DOC_WW_Pin1_4	268	273	PF00397	0.586
DOC_WW_Pin1_4	280	285	PF00397	0.268
DOC_WW_Pin1_4	436	441	PF00397	0.408
DOC_WW_Pin1_4	506	511	PF00397	0.477
DOC_WW_Pin1_4	57	62	PF00397	0.723
DOC_WW_Pin1_4	67	72	PF00397	0.692
DOC_WW_Pin1_4	74	79	PF00397	0.687
DOC_WW_Pin1_4	910	915	PF00397	0.601
LIG_14-3-3_CanoR_1	108	113	PF00244	0.549
LIG_14-3-3_CanoR_1	192	201	PF00244	0.766
LIG_14-3-3_CanoR_1	326	335	PF00244	0.368
LIG_14-3-3_CanoR_1	672	677	PF00244	0.604
LIG_14-3-3_CanoR_1	772	780	PF00244	0.570
LIG_14-3-3_CanoR_1	892	898	PF00244	0.519
LIG_14-3-3_CanoR_1	906	914	PF00244	0.530
LIG_14-3-3_CanoR_1	919	923	PF00244	0.454
LIG_Actin_WH2_2	246	263	PF00022	0.594
LIG_Actin_WH2_2	313	328	PF00022	0.335
LIG_Actin_WH2_2	723	738	PF00022	0.547
LIG_BRCT_BRCA1_1	425	429	PF00533	0.335
LIG_BRCT_BRCA1_1	90	94	PF00533	0.645
LIG_CtBP_PxDLS_1	706	710	PF00389	0.498
LIG_EH_1	99	103	PF12763	0.473
LIG_FHA_1	231	237	PF00498	0.607
LIG_FHA_1	308	314	PF00498	0.335
LIG_FHA_1	330	336	PF00498	0.335
LIG_FHA_1	475	481	PF00498	0.588
LIG_FHA_1	591	597	PF00498	0.524
LIG_FHA_1	636	642	PF00498	0.571
LIG_FHA_1	669	675	PF00498	0.700
LIG_FHA_1	67	73	PF00498	0.511
LIG_FHA_1	713	719	PF00498	0.638
LIG_FHA_1	827	833	PF00498	0.521
LIG_FHA_1	84	90	PF00498	0.675
LIG_FHA_1	849	855	PF00498	0.384
LIG_FHA_2	265	271	PF00498	0.532
LIG_FHA_2	715	721	PF00498	0.523
LIG_Integrin_isoDGR_2	103	105	PF01839	0.642
LIG_LIR_Apic_2	33	37	PF02991	0.577
LIG_LIR_Apic_2	481	485	PF02991	0.419
LIG_LIR_Apic_2	760	765	PF02991	0.414
LIG_LIR_Apic_2	96	101	PF02991	0.454
LIG_LIR_Gen_1	244	253	PF02991	0.603
LIG_LIR_Gen_1	255	265	PF02991	0.638
LIG_LIR_Gen_1	411	419	PF02991	0.335
LIG_LIR_Gen_1	464	474	PF02991	0.578
LIG_LIR_LC3C_4	877	881	PF02991	0.497
LIG_LIR_Nem_3	244	249	PF02991	0.553
LIG_LIR_Nem_3	255	260	PF02991	0.580
LIG_LIR_Nem_3	411	415	PF02991	0.328
LIG_LIR_Nem_3	426	432	PF02991	0.335
LIG_LIR_Nem_3	464	469	PF02991	0.581
LIG_LIR_Nem_3	682	686	PF02991	0.419
LIG_LIR_Nem_3	88	93	PF02991	0.716
LIG_PCNA_yPIPBox_3	250	261	PF02747	0.629
LIG_PCNA_yPIPBox_3	789	800	PF02747	0.541
LIG_PDZ_Class_2	930	935	PF00595	0.653
LIG_Pex14_2	118	122	PF04695	0.539
LIG_Pex14_2	345	349	PF04695	0.321
LIG_Pex14_2	94	98	PF04695	0.496
LIG_SH2_CRK	123	127	PF00017	0.595
LIG_SH2_CRK	257	261	PF00017	0.633
LIG_SH2_CRK	90	94	PF00017	0.678
LIG_SH2_GRB2like	248	251	PF00017	0.620
LIG_SH2_SRC	315	318	PF00017	0.190
LIG_SH2_STAP1	424	428	PF00017	0.447
LIG_SH2_STAP1	836	840	PF00017	0.473
LIG_SH2_STAT3	882	885	PF00017	0.570
LIG_SH2_STAT5	248	251	PF00017	0.557
LIG_SH2_STAT5	315	318	PF00017	0.252
LIG_SH2_STAT5	482	485	PF00017	0.418
LIG_SH2_STAT5	821	824	PF00017	0.483
LIG_SH2_STAT5	867	870	PF00017	0.357
LIG_SH3_3	123	129	PF00018	0.539
LIG_SH3_3	16	22	PF00018	0.729
LIG_SH3_3	269	275	PF00018	0.688
LIG_SH3_3	469	475	PF00018	0.566
LIG_SH3_3	578	584	PF00018	0.459
LIG_SH3_3	671	677	PF00018	0.495
LIG_SH3_3	802	808	PF00018	0.411
LIG_SH3_CIN85_PxpxPR_1	22	27	PF14604	0.695
LIG_SUMO_SIM_anti_2	369	375	PF11976	0.331
LIG_SUMO_SIM_anti_2	579	584	PF11976	0.565
LIG_SUMO_SIM_par_1	369	375	PF11976	0.294
LIG_SUMO_SIM_par_1	638	644	PF11976	0.597
LIG_SUMO_SIM_par_1	662	668	PF11976	0.686
LIG_SUMO_SIM_par_1	710	715	PF11976	0.501
LIG_SUMO_SIM_par_1	850	855	PF11976	0.375
LIG_SUMO_SIM_par_1	929	934	PF11976	0.474
LIG_TRAF2_1	162	165	PF00917	0.447
MOD_CDK_SPxK_1	436	442	PF00069	0.408
MOD_CDK_SPxK_1	506	512	PF00069	0.533
MOD_CDK_SPxxK_3	280	287	PF00069	0.190
MOD_CK1_1	178	184	PF00069	0.713
MOD_CK1_1	304	310	PF00069	0.378
MOD_CK1_1	627	633	PF00069	0.737
MOD_CK1_1	70	76	PF00069	0.750
MOD_CK1_1	752	758	PF00069	0.423
MOD_CK2_1	132	138	PF00069	0.586
MOD_CK2_1	197	203	PF00069	0.584
MOD_CK2_1	676	682	PF00069	0.537
MOD_CK2_1	891	897	PF00069	0.412
MOD_GlcNHglycan	14	17	PF01048	0.708
MOD_GlcNHglycan	178	181	PF01048	0.708
MOD_GlcNHglycan	195	198	PF01048	0.778
MOD_GlcNHglycan	303	306	PF01048	0.371
MOD_GlcNHglycan	523	526	PF01048	0.534
MOD_GlcNHglycan	620	623	PF01048	0.776
MOD_GlcNHglycan	630	633	PF01048	0.707
MOD_GlcNHglycan	647	650	PF01048	0.681
MOD_GlcNHglycan	678	681	PF01048	0.568
MOD_GlcNHglycan	893	896	PF01048	0.407
MOD_GlcNHglycan	90	93	PF01048	0.609
MOD_GSK3_1	106	113	PF00069	0.423
MOD_GSK3_1	176	183	PF00069	0.773
MOD_GSK3_1	188	195	PF00069	0.671
MOD_GSK3_1	260	267	PF00069	0.522
MOD_GSK3_1	274	281	PF00069	0.593
MOD_GSK3_1	39	46	PF00069	0.698
MOD_GSK3_1	413	420	PF00069	0.382
MOD_GSK3_1	474	481	PF00069	0.567
MOD_GSK3_1	500	507	PF00069	0.431
MOD_GSK3_1	517	524	PF00069	0.600
MOD_GSK3_1	624	631	PF00069	0.732
MOD_GSK3_1	643	650	PF00069	0.529
MOD_GSK3_1	66	73	PF00069	0.712
MOD_GSK3_1	668	675	PF00069	0.683
MOD_GSK3_1	685	692	PF00069	0.488
MOD_GSK3_1	710	717	PF00069	0.615
MOD_GSK3_1	74	81	PF00069	0.661
MOD_GSK3_1	745	752	PF00069	0.448
MOD_GSK3_1	844	851	PF00069	0.403
MOD_N-GLC_1	475	480	PF02516	0.551
MOD_N-GLC_1	745	750	PF02516	0.472
MOD_N-GLC_1	752	757	PF02516	0.425
MOD_N-GLC_1	923	928	PF02516	0.577
MOD_NEK2_1	106	111	PF00069	0.517
MOD_NEK2_1	260	265	PF00069	0.418
MOD_NEK2_1	329	334	PF00069	0.335
MOD_NEK2_1	43	48	PF00069	0.683
MOD_NEK2_1	517	522	PF00069	0.505
MOD_NEK2_1	712	717	PF00069	0.569
MOD_NEK2_1	814	819	PF00069	0.597
MOD_NEK2_1	848	853	PF00069	0.389
MOD_NEK2_1	94	99	PF00069	0.588
MOD_NEK2_2	341	346	PF00069	0.354
MOD_NEK2_2	655	660	PF00069	0.670
MOD_NEK2_2	765	770	PF00069	0.496
MOD_NEK2_2	836	841	PF00069	0.376
MOD_PIKK_1	146	152	PF00454	0.614
MOD_PIKK_1	215	221	PF00454	0.615
MOD_PKA_1	672	678	PF00069	0.594
MOD_PKA_2	107	113	PF00069	0.499
MOD_PKA_2	193	199	PF00069	0.744
MOD_PKA_2	260	266	PF00069	0.649
MOD_PKA_2	628	634	PF00069	0.750
MOD_PKA_2	672	678	PF00069	0.630
MOD_PKA_2	771	777	PF00069	0.628
MOD_PKA_2	891	897	PF00069	0.480
MOD_PKA_2	905	911	PF00069	0.503
MOD_PKA_2	918	924	PF00069	0.410
MOD_PKA_2	94	100	PF00069	0.577
MOD_PKB_1	670	678	PF00069	0.613
MOD_Plk_1	374	380	PF00069	0.335
MOD_Plk_1	39	45	PF00069	0.560
MOD_Plk_1	417	423	PF00069	0.381
MOD_Plk_1	475	481	PF00069	0.503
MOD_Plk_1	504	510	PF00069	0.400
MOD_Plk_1	765	771	PF00069	0.541
MOD_Plk_2-3	374	380	PF00069	0.335
MOD_Plk_4	260	266	PF00069	0.386
MOD_Plk_4	307	313	PF00069	0.373
MOD_Plk_4	374	380	PF00069	0.335
MOD_Plk_4	423	429	PF00069	0.346
MOD_Plk_4	636	642	PF00069	0.526
MOD_Plk_4	705	711	PF00069	0.472
MOD_Plk_4	765	771	PF00069	0.522
MOD_Plk_4	817	823	PF00069	0.438
MOD_Plk_4	848	854	PF00069	0.384
MOD_Plk_4	94	100	PF00069	0.550
MOD_ProDKin_1	132	138	PF00069	0.586
MOD_ProDKin_1	268	274	PF00069	0.588
MOD_ProDKin_1	280	286	PF00069	0.268
MOD_ProDKin_1	436	442	PF00069	0.408
MOD_ProDKin_1	506	512	PF00069	0.487
MOD_ProDKin_1	57	63	PF00069	0.725
MOD_ProDKin_1	67	73	PF00069	0.693
MOD_ProDKin_1	74	80	PF00069	0.689
MOD_ProDKin_1	910	916	PF00069	0.606
MOD_SUMO_rev_2	165	172	PF00179	0.405
MOD_SUMO_rev_2	489	498	PF00179	0.427
TRG_DiLeu_BaEn_3	704	710	PF01217	0.553
TRG_DiLeu_BaLyEn_6	542	547	PF01217	0.639
TRG_DiLeu_BaLyEn_6	795	800	PF01217	0.439
TRG_ENDOCYTIC_2	123	126	PF00928	0.615
TRG_ENDOCYTIC_2	257	260	PF00928	0.523
TRG_ENDOCYTIC_2	317	320	PF00928	0.479
TRG_ENDOCYTIC_2	424	427	PF00928	0.293
TRG_ENDOCYTIC_2	90	93	PF00928	0.653
TRG_ER_diArg_1	156	158	PF00400	0.672
TRG_ER_diArg_1	225	227	PF00400	0.592
TRG_ER_diArg_1	24	27	PF00400	0.526
TRG_ER_diArg_1	456	458	PF00400	0.493
TRG_ER_diArg_1	485	488	PF00400	0.458
TRG_ER_diArg_1	597	600	PF00400	0.417
TRG_ER_diArg_1	670	673	PF00400	0.690
TRG_ER_diArg_1	771	773	PF00400	0.494
TRG_ER_diArg_1	891	893	PF00400	0.575
TRG_NLS_MonoExtC_3	183	189	PF00514	0.628
TRG_NLS_MonoExtC_3	668	673	PF00514	0.733
TRG_Pf-PMV_PEXEL_1	251	255	PF00026	0.597
TRG_Pf-PMV_PEXEL_1	604	609	PF00026	0.475
TRG_Pf-PMV_PEXEL_1	700	704	PF00026	0.583
TRG_Pf-PMV_PEXEL_1	779	783	PF00026	0.433
TRG_Pf-PMV_PEXEL_1	798	803	PF00026	0.417
TRG_Pf-PMV_PEXEL_1	874	878	PF00026	0.472

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PCJ6	Leptomonas seymouri	58%	84%
A0A1X0NSB2	Trypanosomatidae	50%	100%
A0A3Q8IGY7	Leishmania donovani	90%	100%
A0A422NKI2	Trypanosoma rangeli	49%	100%
A4HGV5	Leishmania braziliensis	79%	100%
A4I3Y5	Leishmania infantum	90%	100%
D0A956	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	47%	100%
Q383A1	Trypanosoma brucei brucei (strain 927/4 GUTat10.1)	47%	100%
Q4Q810	Leishmania major	89%	100%
V5BL79	Trypanosoma cruzi	50%	100%