Adenine DNA glycosylase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

A/G-specific adenine glycosylase, putative

Species:

Leishmania mexicana

UniProt:

E9B035_LEIMU

TriTrypDb:

LmxM.28.2140

Length:

501

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9B035

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9B035

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006259	DNA metabolic process	4	12
GO:0006281	DNA repair	5	12
GO:0006284	base-excision repair	6	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0006950	response to stress	2	12
GO:0006974	DNA damage response	4	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0033554	cellular response to stress	3	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0046483	heterocycle metabolic process	3	12
GO:0050896	response to stimulus	1	12
GO:0051716	cellular response to stimulus	2	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0006298	mismatch repair	6	1

Molecular functions
Term	Name	Level	Count
GO:0000700	mismatch base pair DNA N-glycosylase activity	5	12
GO:0000701	purine-specific mismatch base pair DNA N-glycosylase activity	6	12
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0016787	hydrolase activity	2	12
GO:0016798	hydrolase activity, acting on glycosyl bonds	3	12
GO:0016799	hydrolase activity, hydrolyzing N-glycosyl compounds	4	12
GO:0019104	DNA N-glycosylase activity	4	12
GO:0043167	ion binding	2	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0051536	iron-sulfur cluster binding	3	12
GO:0051539	4 iron, 4 sulfur cluster binding	4	12
GO:0051540	metal cluster binding	2	12
GO:0140097	catalytic activity, acting on DNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:0000702	oxidized base lesion DNA N-glycosylase activity	5	1
GO:0003676	nucleic acid binding	3	4
GO:0003677	DNA binding	4	4
GO:0003684	damaged DNA binding	5	1
GO:0003690	double-stranded DNA binding	5	1
GO:0008534	oxidized purine nucleobase lesion DNA N-glycosylase activity	6	1
GO:0030983	mismatched DNA binding	6	1
GO:0032356	oxidized DNA binding	6	1
GO:0032357	oxidized purine DNA binding	7	1
GO:0034039	8-oxo-7,8-dihydroguanine DNA N-glycosylase activity	7	1
GO:0035485	adenine/guanine mispair binding	7	1
GO:0097159	organic cyclic compound binding	2	4
GO:1901363	heterocyclic compound binding	2	4
GO:0004518	nuclease activity	4	2
GO:0004519	endonuclease activity	5	2
GO:0016788	hydrolase activity, acting on ester bonds	3	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	482	486	PF00656	0.492
CLV_NRD_NRD_1	134	136	PF00675	0.206
CLV_NRD_NRD_1	139	141	PF00675	0.206
CLV_NRD_NRD_1	324	326	PF00675	0.275
CLV_NRD_NRD_1	330	332	PF00675	0.275
CLV_PCSK_KEX2_1	134	136	PF00082	0.206
CLV_PCSK_KEX2_1	303	305	PF00082	0.511
CLV_PCSK_KEX2_1	324	326	PF00082	0.276
CLV_PCSK_KEX2_1	330	332	PF00082	0.276
CLV_PCSK_KEX2_1	414	416	PF00082	0.534
CLV_PCSK_KEX2_1	477	479	PF00082	0.517
CLV_PCSK_PC1ET2_1	303	305	PF00082	0.511
CLV_PCSK_PC1ET2_1	414	416	PF00082	0.417
CLV_PCSK_PC1ET2_1	477	479	PF00082	0.517
CLV_PCSK_SKI1_1	141	145	PF00082	0.226
CLV_PCSK_SKI1_1	153	157	PF00082	0.311
CLV_PCSK_SKI1_1	297	301	PF00082	0.538
CLV_PCSK_SKI1_1	386	390	PF00082	0.284
CLV_PCSK_SKI1_1	437	441	PF00082	0.421
CLV_PCSK_SKI1_1	453	457	PF00082	0.252
DEG_APCC_DBOX_1	374	382	PF00400	0.142
DEG_APCC_DBOX_1	452	460	PF00400	0.351
DEG_SCF_FBW7_1	16	21	PF00400	0.404
DOC_CKS1_1	15	20	PF01111	0.478
DOC_CYCLIN_yCln2_LP_2	361	367	PF00134	0.262
DOC_MAPK_gen_1	303	311	PF00069	0.356
DOC_MAPK_gen_1	373	381	PF00069	0.310
DOC_PP2B_LxvP_1	169	172	PF13499	0.510
DOC_PP4_FxxP_1	187	190	PF00568	0.421
DOC_SPAK_OSR1_1	325	329	PF12202	0.275
DOC_USP7_MATH_1	155	159	PF00917	0.335
DOC_USP7_MATH_1	176	180	PF00917	0.420
DOC_USP7_MATH_1	18	22	PF00917	0.416
DOC_USP7_MATH_1	181	185	PF00917	0.393
DOC_USP7_MATH_1	190	194	PF00917	0.379
DOC_USP7_MATH_1	234	238	PF00917	0.518
DOC_USP7_MATH_1	302	306	PF00917	0.439
DOC_USP7_MATH_1	54	58	PF00917	0.542
DOC_USP7_UBL2_3	457	461	PF12436	0.313
DOC_WD40_RPTOR_TOS_1	463	469	PF00400	0.249
DOC_WW_Pin1_4	14	19	PF00397	0.434
DOC_WW_Pin1_4	230	235	PF00397	0.479
DOC_WW_Pin1_4	485	490	PF00397	0.606
LIG_14-3-3_CanoR_1	386	396	PF00244	0.271
LIG_14-3-3_CanoR_1	50	54	PF00244	0.470
LIG_BIR_II_1	1	5	PF00653	0.601
LIG_BIR_III_4	406	410	PF00653	0.379
LIG_BRCT_BRCA1_1	183	187	PF00533	0.406
LIG_deltaCOP1_diTrp_1	14	22	PF00928	0.365
LIG_FHA_1	234	240	PF00498	0.498
LIG_FHA_2	117	123	PF00498	0.522
LIG_FHA_2	91	97	PF00498	0.434
LIG_LIR_Apic_2	14	18	PF02991	0.476
LIG_LIR_Apic_2	184	190	PF02991	0.421
LIG_LIR_Nem_3	26	31	PF02991	0.359
LIG_LIR_Nem_3	342	346	PF02991	0.281
LIG_LIR_Nem_3	75	81	PF02991	0.487
LIG_LRP6_Inhibitor_1	194	200	PF00058	0.310
LIG_PCNA_yPIPBox_3	29	39	PF02747	0.366
LIG_PCNA_yPIPBox_3	330	341	PF02747	0.262
LIG_Pex14_1	105	109	PF04695	0.406
LIG_SH2_CRK	28	32	PF00017	0.344
LIG_SH2_NCK_1	177	181	PF00017	0.417
LIG_SH2_STAP1	102	106	PF00017	0.382
LIG_SH2_STAP1	177	181	PF00017	0.417
LIG_SH2_STAT5	138	141	PF00017	0.405
LIG_SH2_STAT5	145	148	PF00017	0.408
LIG_SH2_STAT5	177	180	PF00017	0.406
LIG_SH3_3	210	216	PF00018	0.432
LIG_SH3_3	255	261	PF00018	0.315
LIG_SH3_3	326	332	PF00018	0.300
LIG_SH3_3	398	404	PF00018	0.366
LIG_SUMO_SIM_anti_2	395	400	PF11976	0.251
LIG_SUMO_SIM_par_1	363	369	PF11976	0.429
LIG_TRAF2_1	163	166	PF00917	0.510
LIG_TRAF2_1	30	33	PF00917	0.354
LIG_TRAF2_1	479	482	PF00917	0.596
LIG_UBA3_1	455	461	PF00899	0.370
LIG_WRC_WIRS_1	19	24	PF05994	0.369
MOD_CDC14_SPxK_1	488	491	PF00782	0.612
MOD_CDK_SPxK_1	485	491	PF00069	0.609
MOD_CDK_SPxxK_3	485	492	PF00069	0.611
MOD_CK1_1	2	8	PF00069	0.666
MOD_CK1_1	21	27	PF00069	0.534
MOD_CK1_1	233	239	PF00069	0.565
MOD_CK1_1	366	372	PF00069	0.429
MOD_CK1_1	72	78	PF00069	0.476
MOD_CK1_1	90	96	PF00069	0.335
MOD_CK2_1	116	122	PF00069	0.481
MOD_CK2_1	160	166	PF00069	0.451
MOD_CK2_1	27	33	PF00069	0.447
MOD_CK2_1	302	308	PF00069	0.351
MOD_CK2_1	90	96	PF00069	0.480
MOD_Cter_Amidation	475	478	PF01082	0.616
MOD_GlcNHglycan	178	181	PF01048	0.269
MOD_GlcNHglycan	236	239	PF01048	0.507
MOD_GlcNHglycan	243	247	PF01048	0.376
MOD_GlcNHglycan	261	264	PF01048	0.259
MOD_GlcNHglycan	304	307	PF01048	0.472
MOD_GlcNHglycan	314	318	PF01048	0.386
MOD_GlcNHglycan	348	351	PF01048	0.342
MOD_GlcNHglycan	4	7	PF01048	0.657
MOD_GlcNHglycan	56	59	PF01048	0.652
MOD_GlcNHglycan	60	63	PF01048	0.598
MOD_GlcNHglycan	71	74	PF01048	0.420
MOD_GSK3_1	14	21	PF00069	0.532
MOD_GSK3_1	156	163	PF00069	0.504
MOD_GSK3_1	230	237	PF00069	0.399
MOD_GSK3_1	313	320	PF00069	0.322
MOD_GSK3_1	342	349	PF00069	0.248
MOD_GSK3_1	481	488	PF00069	0.632
MOD_GSK3_1	54	61	PF00069	0.501
MOD_GSK3_1	68	75	PF00069	0.590
MOD_GSK3_1	83	90	PF00069	0.177
MOD_N-GLC_1	346	351	PF02516	0.354
MOD_NEK2_1	254	259	PF00069	0.490
MOD_NEK2_1	266	271	PF00069	0.314
MOD_NEK2_1	381	386	PF00069	0.387
MOD_NEK2_2	7	12	PF00069	0.444
MOD_PIKK_1	161	167	PF00454	0.476
MOD_PIKK_1	87	93	PF00454	0.406
MOD_PK_1	108	114	PF00069	0.520
MOD_PK_1	461	467	PF00069	0.343
MOD_PKA_2	2	8	PF00069	0.517
MOD_PKA_2	259	265	PF00069	0.351
MOD_PKA_2	374	380	PF00069	0.306
MOD_PKA_2	470	476	PF00069	0.498
MOD_PKA_2	49	55	PF00069	0.470
MOD_PKB_1	151	159	PF00069	0.335
MOD_Plk_4	122	128	PF00069	0.512
MOD_Plk_4	277	283	PF00069	0.184
MOD_Plk_4	363	369	PF00069	0.306
MOD_Plk_4	374	380	PF00069	0.341
MOD_Plk_4	94	100	PF00069	0.414
MOD_ProDKin_1	14	20	PF00069	0.436
MOD_ProDKin_1	230	236	PF00069	0.474
MOD_ProDKin_1	485	491	PF00069	0.609
MOD_SUMO_rev_2	118	126	PF00179	0.512
MOD_SUMO_rev_2	208	216	PF00179	0.406
MOD_SUMO_rev_2	464	470	PF00179	0.418
TRG_DiLeu_BaLyEn_6	356	361	PF01217	0.262
TRG_ENDOCYTIC_2	28	31	PF00928	0.348
TRG_ER_diArg_1	133	135	PF00400	0.406
TRG_ER_diArg_1	324	326	PF00400	0.276
TRG_ER_diArg_1	329	331	PF00400	0.276
TRG_ER_diArg_1	373	376	PF00400	0.262
TRG_Pf-PMV_PEXEL_1	144	148	PF00026	0.324
TRG_Pf-PMV_PEXEL_1	478	482	PF00026	0.550

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IL90	Leptomonas seymouri	60%	98%
A0A0S4IRI2	Bodo saltans	37%	95%
A0A1X0NR87	Trypanosomatidae	44%	100%
A0A3S7X1P4	Leishmania donovani	93%	100%
A0A422N7D8	Trypanosoma rangeli	42%	100%
A4HGR8	Leishmania braziliensis	80%	100%
A4I3U3	Leishmania infantum	93%	100%
D0A913	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	43%	100%
F4JRF4	Arabidopsis thaliana	33%	80%
O31584	Bacillus subtilis (strain 168)	29%	100%
P17802	Escherichia coli (strain K12)	30%	100%
P44320	Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)	28%	100%
P57617	Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS)	25%	100%
P83847	Geobacillus stearothermophilus	32%	100%
Q05869	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)	28%	100%
Q10159	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	31%	100%
Q4Q851	Leishmania major	91%	100%
Q8R5G2	Rattus norvegicus	31%	97%
Q99P21	Mus musculus	30%	97%
Q9UIF7	Homo sapiens	32%	92%
V5B3S1	Trypanosoma cruzi	42%	100%