DNA polymerase kappa

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DNA polymerase kappa, putative

Species:

Leishmania mexicana

UniProt:

E9AZW3_LEIMU

TriTrypDb:

LmxM.28.1430

Length:

463

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 18

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 19
NetGPI	no	yes: 0, no: 19

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AZW3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AZW3

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	20
GO:0006259	DNA metabolic process	4	20
GO:0006260	DNA replication	5	13
GO:0006281	DNA repair	5	20
GO:0006725	cellular aromatic compound metabolic process	3	20
GO:0006807	nitrogen compound metabolic process	2	20
GO:0006950	response to stress	2	20
GO:0006974	DNA damage response	4	20
GO:0008152	metabolic process	1	20
GO:0009987	cellular process	1	20
GO:0033554	cellular response to stress	3	20
GO:0034641	cellular nitrogen compound metabolic process	3	20
GO:0043170	macromolecule metabolic process	3	20
GO:0044237	cellular metabolic process	2	20
GO:0044238	primary metabolic process	2	20
GO:0044260	obsolete cellular macromolecule metabolic process	3	20
GO:0046483	heterocycle metabolic process	3	20
GO:0050896	response to stimulus	1	20
GO:0051716	cellular response to stimulus	2	20
GO:0071704	organic substance metabolic process	2	20
GO:0090304	nucleic acid metabolic process	4	20
GO:1901360	organic cyclic compound metabolic process	3	20
GO:0000731	DNA synthesis involved in DNA repair	6	1
GO:0006301	postreplication repair	6	1
GO:0009058	biosynthetic process	2	1
GO:0009059	macromolecule biosynthetic process	4	1
GO:0018130	heterocycle biosynthetic process	4	1
GO:0019438	aromatic compound biosynthetic process	4	1
GO:0019985	translesion synthesis	7	1
GO:0034654	nucleobase-containing compound biosynthetic process	4	1
GO:0042276	error-prone translesion synthesis	8	1
GO:0044249	cellular biosynthetic process	3	1
GO:0044271	cellular nitrogen compound biosynthetic process	4	1
GO:0071897	DNA biosynthetic process	5	1
GO:1901362	organic cyclic compound biosynthetic process	4	1
GO:1901576	organic substance biosynthetic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	20
GO:0003677	DNA binding	4	20
GO:0003684	damaged DNA binding	5	20
GO:0003824	catalytic activity	1	19
GO:0003887	DNA-directed DNA polymerase activity	5	19
GO:0005488	binding	1	20
GO:0016740	transferase activity	2	19
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	19
GO:0016779	nucleotidyltransferase activity	4	19
GO:0034061	DNA polymerase activity	4	19
GO:0043167	ion binding	2	13
GO:0043169	cation binding	3	13
GO:0046872	metal ion binding	4	13
GO:0097159	organic cyclic compound binding	2	20
GO:0140097	catalytic activity, acting on DNA	3	19
GO:0140640	catalytic activity, acting on a nucleic acid	2	19
GO:1901363	heterocyclic compound binding	2	20
GO:0016491	oxidoreductase activity	2	6

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	116	118	PF00675	0.139
CLV_NRD_NRD_1	196	198	PF00675	0.210
CLV_NRD_NRD_1	386	388	PF00675	0.136
CLV_NRD_NRD_1	432	434	PF00675	0.570
CLV_NRD_NRD_1	66	68	PF00675	0.160
CLV_PCSK_KEX2_1	115	117	PF00082	0.353
CLV_PCSK_KEX2_1	386	388	PF00082	0.136
CLV_PCSK_KEX2_1	432	434	PF00082	0.461
CLV_PCSK_KEX2_1	66	68	PF00082	0.160
CLV_PCSK_SKI1_1	192	196	PF00082	0.375
CLV_PCSK_SKI1_1	284	288	PF00082	0.130
CLV_PCSK_SKI1_1	295	299	PF00082	0.247
CLV_PCSK_SKI1_1	426	430	PF00082	0.494
DEG_Nend_Nbox_1	1	3	PF02207	0.334
DOC_MAPK_gen_1	367	376	PF00069	0.391
DOC_MAPK_gen_1	49	57	PF00069	0.414
DOC_MAPK_MEF2A_6	170	177	PF00069	0.521
DOC_MAPK_MEF2A_6	367	376	PF00069	0.424
DOC_MAPK_MEF2A_6	49	57	PF00069	0.377
DOC_PP1_RVXF_1	319	326	PF00149	0.385
DOC_USP7_MATH_1	103	107	PF00917	0.481
DOC_USP7_MATH_1	258	262	PF00917	0.625
DOC_USP7_MATH_1	278	282	PF00917	0.272
DOC_USP7_UBL2_3	280	284	PF12436	0.426
DOC_USP7_UBL2_3	399	403	PF12436	0.434
DOC_WW_Pin1_4	262	267	PF00397	0.692
DOC_WW_Pin1_4	427	432	PF00397	0.626
DOC_WW_Pin1_4	435	440	PF00397	0.555
LIG_14-3-3_CanoR_1	259	269	PF00244	0.536
LIG_14-3-3_CanoR_1	277	286	PF00244	0.220
LIG_14-3-3_CanoR_1	448	453	PF00244	0.445
LIG_BRCT_BRCA1_1	328	332	PF00533	0.339
LIG_FHA_1	318	324	PF00498	0.462
LIG_FHA_1	34	40	PF00498	0.460
LIG_FHA_1	90	96	PF00498	0.390
LIG_FHA_2	154	160	PF00498	0.309
LIG_FHA_2	188	194	PF00498	0.201
LIG_FHA_2	221	227	PF00498	0.370
LIG_FHA_2	452	458	PF00498	0.585
LIG_FHA_2	99	105	PF00498	0.352
LIG_Integrin_RGD_1	412	414	PF01839	0.435
LIG_LIR_Apic_2	54	59	PF02991	0.439
LIG_LIR_Gen_1	133	143	PF02991	0.453
LIG_LIR_Gen_1	23	32	PF02991	0.341
LIG_LIR_Gen_1	296	306	PF02991	0.473
LIG_LIR_Gen_1	345	355	PF02991	0.388
LIG_LIR_Gen_1	80	91	PF02991	0.382
LIG_LIR_Nem_3	133	139	PF02991	0.453
LIG_LIR_Nem_3	207	213	PF02991	0.347
LIG_LIR_Nem_3	23	27	PF02991	0.341
LIG_LIR_Nem_3	296	302	PF02991	0.496
LIG_LIR_Nem_3	345	351	PF02991	0.364
LIG_LIR_Nem_3	368	372	PF02991	0.433
LIG_LIR_Nem_3	80	86	PF02991	0.396
LIG_PDZ_Class_1	458	463	PF00595	0.448
LIG_SH2_CRK	24	28	PF00017	0.349
LIG_SH2_NCK_1	3	7	PF00017	0.391
LIG_SH2_PTP2	83	86	PF00017	0.364
LIG_SH2_SRC	79	82	PF00017	0.415
LIG_SH2_STAT3	408	411	PF00017	0.433
LIG_SH2_STAT5	163	166	PF00017	0.397
LIG_SH2_STAT5	203	206	PF00017	0.337
LIG_SH2_STAT5	213	216	PF00017	0.331
LIG_SH2_STAT5	32	35	PF00017	0.385
LIG_SH2_STAT5	326	329	PF00017	0.389
LIG_SH2_STAT5	83	86	PF00017	0.367
LIG_SH2_STAT5	98	101	PF00017	0.424
LIG_SH3_1	433	439	PF00018	0.452
LIG_SH3_3	140	146	PF00018	0.556
LIG_SH3_3	355	361	PF00018	0.343
LIG_SH3_3	433	439	PF00018	0.511
LIG_SH3_4	145	152	PF00018	0.301
LIG_SUMO_SIM_par_1	150	156	PF11976	0.245
LIG_TRAF2_1	156	159	PF00917	0.431
LIG_TRAF2_1	5	8	PF00917	0.487
LIG_TRAF2_2	409	414	PF00917	0.507
LIG_WW_3	256	260	PF00397	0.433
LIG_WW_3	58	62	PF00397	0.323
MOD_CDK_SPK_2	427	432	PF00069	0.446
MOD_CDK_SPxK_1	427	433	PF00069	0.448
MOD_CDK_SPxxK_3	435	442	PF00069	0.454
MOD_CK1_1	342	348	PF00069	0.377
MOD_CK2_1	10	16	PF00069	0.339
MOD_CK2_1	153	159	PF00069	0.435
MOD_CK2_1	225	231	PF00069	0.552
MOD_CK2_1	451	457	PF00069	0.665
MOD_Cter_Amidation	384	387	PF01082	0.138
MOD_GlcNHglycan	104	108	PF01048	0.249
MOD_GlcNHglycan	126	129	PF01048	0.223
MOD_GlcNHglycan	239	242	PF01048	0.689
MOD_GlcNHglycan	248	251	PF01048	0.427
MOD_GlcNHglycan	260	263	PF01048	0.682
MOD_GlcNHglycan	266	269	PF01048	0.645
MOD_GlcNHglycan	40	43	PF01048	0.243
MOD_GlcNHglycan	444	447	PF01048	0.580
MOD_GlcNHglycan	460	463	PF01048	0.463
MOD_GSK3_1	233	240	PF00069	0.455
MOD_GSK3_1	242	249	PF00069	0.495
MOD_GSK3_1	258	265	PF00069	0.436
MOD_GSK3_1	89	96	PF00069	0.389
MOD_NEK2_1	10	15	PF00069	0.442
MOD_NEK2_1	124	129	PF00069	0.438
MOD_NEK2_1	212	217	PF00069	0.234
MOD_NEK2_1	220	225	PF00069	0.370
MOD_NEK2_1	26	31	PF00069	0.408
MOD_NEK2_1	325	330	PF00069	0.521
MOD_NEK2_2	187	192	PF00069	0.488
MOD_PIKK_1	119	125	PF00454	0.425
MOD_PIKK_1	260	266	PF00454	0.600
MOD_PKA_1	386	392	PF00069	0.650
MOD_PKA_2	258	264	PF00069	0.580
MOD_PKA_2	385	391	PF00069	0.650
MOD_Plk_1	10	16	PF00069	0.323
MOD_Plk_1	119	125	PF00069	0.323
MOD_Plk_1	206	212	PF00069	0.424
MOD_Plk_1	89	95	PF00069	0.386
MOD_Plk_2-3	89	95	PF00069	0.386
MOD_Plk_4	284	290	PF00069	0.323
MOD_Plk_4	51	57	PF00069	0.369
MOD_ProDKin_1	262	268	PF00069	0.690
MOD_ProDKin_1	427	433	PF00069	0.628
MOD_ProDKin_1	435	441	PF00069	0.555
TRG_DiLeu_BaEn_4	119	125	PF01217	0.323
TRG_DiLeu_BaEn_4	7	13	PF01217	0.323
TRG_DiLeu_BaLyEn_6	367	372	PF01217	0.456
TRG_ENDOCYTIC_2	213	216	PF00928	0.417
TRG_ENDOCYTIC_2	24	27	PF00928	0.349
TRG_ENDOCYTIC_2	3	6	PF00928	0.396
TRG_ENDOCYTIC_2	352	355	PF00928	0.415
TRG_ENDOCYTIC_2	83	86	PF00928	0.345
TRG_ER_diArg_1	114	117	PF00400	0.336
TRG_ER_diArg_1	154	157	PF00400	0.323
TRG_ER_diArg_1	431	433	PF00400	0.453
TRG_ER_diArg_1	65	67	PF00400	0.360
TRG_NES_CRM1_1	159	171	PF08389	0.339
TRG_Pf-PMV_PEXEL_1	303	307	PF00026	0.281

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I6L1	Leptomonas seymouri	43%	70%
A0A0N1PDB9	Leptomonas seymouri	65%	74%
A0A1X0NYM2	Trypanosomatidae	41%	76%
A0A1X0NZ35	Trypanosomatidae	40%	90%
A0A3R7KKS0	Trypanosoma rangeli	42%	100%
A0A3S7X1H5	Leishmania donovani	83%	86%
A0A3S7X1J7	Leishmania donovani	44%	77%
A0A422MU96	Trypanosoma rangeli	43%	100%
A0AK80	Listeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / CIP 8149 / NCTC 11857 / SLCC 5334 / V8)	27%	100%
A4I3M1	Leishmania infantum	44%	77%
A4I3M2	Leishmania infantum	83%	100%
A4QFK8	Corynebacterium glutamicum (strain R)	24%	99%
A9A3A9	Nitrosopumilus maritimus (strain SCM1)	26%	100%
B8DBV7	Listeria monocytogenes serotype 4a (strain HCC23)	27%	100%
B8FBE8	Desulfatibacillum aliphaticivorans	26%	100%
C1KWR9	Listeria monocytogenes serotype 4b (strain CLIP80459)	27%	100%
D0A846	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	35%	81%
D0A847	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	38%	81%
D0A848	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	36%	81%
D0A849	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	37%	81%
D0A851	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	36%	81%
D0A852	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	36%	81%
E9AZW2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	44%	77%
P58965	Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4)	27%	100%
P96022	Saccharolobus solfataricus	24%	100%
Q2RJ46	Moorella thermoacetica (strain ATCC 39073 / JCM 9320)	24%	100%
Q4JB80	Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)	24%	100%
Q4Q8C4	Leishmania major	44%	77%
Q71Y46	Listeria monocytogenes serotype 4b (strain F2365)	27%	100%
Q8NNP4	Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)	24%	99%
Q8Y5T0	Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)	27%	100%
Q92A40	Listeria innocua serovar 6a (strain ATCC BAA-680 / CLIP 11262)	27%	100%
Q974T8	Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)	24%	100%
Q98LV1	Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)	28%	100%