DNA polymerase kappa

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DNA polymerase kappa, putative

Species:

Leishmania mexicana

UniProt:

E9AZW2_LEIMU

TriTrypDb:

LmxM.28.1420

Length:

598

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 22
NetGPI	no	yes: 0, no: 22

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9AZW2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AZW2

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	23
GO:0006259	DNA metabolic process	4	23
GO:0006281	DNA repair	5	23
GO:0006725	cellular aromatic compound metabolic process	3	23
GO:0006807	nitrogen compound metabolic process	2	23
GO:0006950	response to stress	2	23
GO:0006974	DNA damage response	4	23
GO:0008152	metabolic process	1	23
GO:0009987	cellular process	1	23
GO:0033554	cellular response to stress	3	23
GO:0034641	cellular nitrogen compound metabolic process	3	23
GO:0043170	macromolecule metabolic process	3	23
GO:0044237	cellular metabolic process	2	23
GO:0044238	primary metabolic process	2	23
GO:0044260	obsolete cellular macromolecule metabolic process	3	23
GO:0046483	heterocycle metabolic process	3	23
GO:0050896	response to stimulus	1	23
GO:0051716	cellular response to stimulus	2	23
GO:0071704	organic substance metabolic process	2	23
GO:0090304	nucleic acid metabolic process	4	23
GO:1901360	organic cyclic compound metabolic process	3	23
GO:0006260	DNA replication	5	11
GO:0000731	DNA synthesis involved in DNA repair	6	2
GO:0006301	postreplication repair	6	2
GO:0009058	biosynthetic process	2	2
GO:0009059	macromolecule biosynthetic process	4	2
GO:0018130	heterocycle biosynthetic process	4	2
GO:0019438	aromatic compound biosynthetic process	4	2
GO:0019985	translesion synthesis	7	2
GO:0034654	nucleobase-containing compound biosynthetic process	4	2
GO:0042276	error-prone translesion synthesis	8	2
GO:0044249	cellular biosynthetic process	3	2
GO:0044271	cellular nitrogen compound biosynthetic process	4	2
GO:0071897	DNA biosynthetic process	5	2
GO:1901362	organic cyclic compound biosynthetic process	4	2
GO:1901576	organic substance biosynthetic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	23
GO:0003677	DNA binding	4	23
GO:0003684	damaged DNA binding	5	23
GO:0003824	catalytic activity	1	23
GO:0003887	DNA-directed DNA polymerase activity	5	23
GO:0005488	binding	1	23
GO:0016740	transferase activity	2	23
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	23
GO:0016779	nucleotidyltransferase activity	4	23
GO:0034061	DNA polymerase activity	4	23
GO:0097159	organic cyclic compound binding	2	23
GO:0140097	catalytic activity, acting on DNA	3	23
GO:0140640	catalytic activity, acting on a nucleic acid	2	23
GO:1901363	heterocyclic compound binding	2	23
GO:0043167	ion binding	2	11
GO:0043169	cation binding	3	11
GO:0046872	metal ion binding	4	11
GO:0016491	oxidoreductase activity	2	6

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	495	499	PF00656	0.535
CLV_NRD_NRD_1	100	102	PF00675	0.458
CLV_NRD_NRD_1	143	145	PF00675	0.330
CLV_NRD_NRD_1	320	322	PF00675	0.418
CLV_NRD_NRD_1	377	379	PF00675	0.501
CLV_NRD_NRD_1	406	408	PF00675	0.318
CLV_NRD_NRD_1	424	426	PF00675	0.215
CLV_NRD_NRD_1	550	552	PF00675	0.739
CLV_NRD_NRD_1	583	585	PF00675	0.724
CLV_NRD_NRD_1	596	598	PF00675	0.470
CLV_PCSK_KEX2_1	142	144	PF00082	0.357
CLV_PCSK_KEX2_1	550	552	PF00082	0.739
CLV_PCSK_KEX2_1	585	587	PF00082	0.683
CLV_PCSK_KEX2_1	596	598	PF00082	0.712
CLV_PCSK_KEX2_1	99	101	PF00082	0.432
CLV_PCSK_PC1ET2_1	550	552	PF00082	0.578
CLV_PCSK_PC1ET2_1	585	587	PF00082	0.656
CLV_PCSK_PC1ET2_1	596	598	PF00082	0.734
CLV_PCSK_PC7_1	139	145	PF00082	0.483
CLV_PCSK_SKI1_1	349	353	PF00082	0.324
CLV_PCSK_SKI1_1	407	411	PF00082	0.308
CLV_PCSK_SKI1_1	425	429	PF00082	0.154
CLV_PCSK_SKI1_1	497	501	PF00082	0.696
CLV_PCSK_SKI1_1	557	561	PF00082	0.593
CLV_PCSK_SKI1_1	578	582	PF00082	0.737
DOC_CYCLIN_RxL_1	404	411	PF00134	0.521
DOC_CYCLIN_yCln2_LP_2	504	510	PF00134	0.528
DOC_MAPK_gen_1	139	148	PF00069	0.313
DOC_MAPK_gen_1	200	208	PF00069	0.439
DOC_MAPK_gen_1	425	431	PF00069	0.440
DOC_MAPK_MEF2A_6	142	150	PF00069	0.352
DOC_MAPK_MEF2A_6	200	208	PF00069	0.435
DOC_MAPK_RevD_3	146	161	PF00069	0.239
DOC_PP1_RVXF_1	55	61	PF00149	0.532
DOC_PP2B_LxvP_1	29	32	PF13499	0.461
DOC_PP4_FxxP_1	392	395	PF00568	0.519
DOC_USP7_MATH_1	254	258	PF00917	0.498
DOC_USP7_MATH_1	32	36	PF00917	0.687
DOC_USP7_MATH_1	382	386	PF00917	0.299
DOC_USP7_MATH_1	525	529	PF00917	0.544
DOC_USP7_MATH_1	532	536	PF00917	0.649
DOC_USP7_UBL2_3	121	125	PF12436	0.347
DOC_USP7_UBL2_3	581	585	PF12436	0.690
DOC_WW_Pin1_4	159	164	PF00397	0.412
DOC_WW_Pin1_4	395	400	PF00397	0.455
DOC_WW_Pin1_4	538	543	PF00397	0.739
LIG_14-3-3_CanoR_1	383	387	PF00244	0.446
LIG_14-3-3_CanoR_1	425	430	PF00244	0.418
LIG_14-3-3_CanoR_1	586	594	PF00244	0.693
LIG_14-3-3_CanoR_1	8	17	PF00244	0.715
LIG_Actin_WH2_1	308	323	PF00022	0.318
LIG_APCC_ABBAyCdc20_2	143	149	PF00400	0.398
LIG_BIR_II_1	1	5	PF00653	0.540
LIG_eIF4E_1	350	356	PF01652	0.382
LIG_eIF4E_1	470	476	PF01652	0.403
LIG_FHA_1	136	142	PF00498	0.349
LIG_FHA_1	160	166	PF00498	0.451
LIG_FHA_1	179	185	PF00498	0.472
LIG_FHA_1	241	247	PF00498	0.406
LIG_FHA_1	282	288	PF00498	0.437
LIG_FHA_1	372	378	PF00498	0.557
LIG_FHA_1	426	432	PF00498	0.482
LIG_FHA_1	447	453	PF00498	0.407
LIG_FHA_1	499	505	PF00498	0.717
LIG_FHA_2	305	311	PF00498	0.324
LIG_FHA_2	482	488	PF00498	0.521
LIG_GBD_Chelix_1	361	369	PF00786	0.275
LIG_HCF-1_HBM_1	347	350	PF13415	0.296
LIG_LIR_Apic_2	389	395	PF02991	0.519
LIG_LIR_Gen_1	112	119	PF02991	0.395
LIG_LIR_Gen_1	151	159	PF02991	0.425
LIG_LIR_Gen_1	231	242	PF02991	0.404
LIG_LIR_Gen_1	400	410	PF02991	0.470
LIG_LIR_Nem_3	112	116	PF02991	0.433
LIG_LIR_Nem_3	151	157	PF02991	0.425
LIG_LIR_Nem_3	212	216	PF02991	0.428
LIG_LIR_Nem_3	231	237	PF02991	0.468
LIG_LIR_Nem_3	252	256	PF02991	0.521
LIG_LIR_Nem_3	400	405	PF02991	0.500
LIG_LIR_Nem_3	472	476	PF02991	0.503
LIG_Pex14_2	402	406	PF04695	0.519
LIG_PTB_Apo_2	264	271	PF02174	0.331
LIG_SH2_NCK_1	154	158	PF00017	0.430
LIG_SH2_PTP2	145	148	PF00017	0.269
LIG_SH2_SRC	145	148	PF00017	0.371
LIG_SH2_SRC	74	77	PF00017	0.355
LIG_SH2_STAP1	230	234	PF00017	0.531
LIG_SH2_STAP1	328	332	PF00017	0.466
LIG_SH2_STAT3	230	233	PF00017	0.519
LIG_SH2_STAT5	145	148	PF00017	0.308
LIG_SH2_STAT5	183	186	PF00017	0.447
LIG_SH2_STAT5	249	252	PF00017	0.496
LIG_SH2_STAT5	314	317	PF00017	0.381
LIG_SH2_STAT5	350	353	PF00017	0.329
LIG_SH2_STAT5	354	357	PF00017	0.322
LIG_SH2_STAT5	364	367	PF00017	0.289
LIG_SH2_STAT5	74	77	PF00017	0.485
LIG_SH3_1	566	572	PF00018	0.553
LIG_SH3_3	291	297	PF00018	0.564
LIG_SH3_3	539	545	PF00018	0.586
LIG_SH3_3	559	565	PF00018	0.559
LIG_SH3_3	566	572	PF00018	0.516
LIG_SH3_4	296	303	PF00018	0.376
LIG_SH3_4	581	588	PF00018	0.470
LIG_SUMO_SIM_anti_2	205	210	PF11976	0.519
LIG_SUMO_SIM_anti_2	302	307	PF11976	0.496
LIG_TRAF2_1	156	159	PF00917	0.477
LIG_TRAF2_1	307	310	PF00917	0.435
LIG_TRAF2_2	507	512	PF00917	0.524
LIG_UBA3_1	351	359	PF00899	0.480
MOD_CDK_SPxxK_3	159	166	PF00069	0.412
MOD_CK1_1	225	231	PF00069	0.519
MOD_CK1_1	446	452	PF00069	0.373
MOD_CK1_1	83	89	PF00069	0.446
MOD_CK2_1	114	120	PF00069	0.427
MOD_CK2_1	304	310	PF00069	0.441
MOD_CK2_1	388	394	PF00069	0.462
MOD_Cter_Amidation	198	201	PF01082	0.244
MOD_GlcNHglycan	116	119	PF01048	0.514
MOD_GlcNHglycan	191	194	PF01048	0.323
MOD_GlcNHglycan	256	259	PF01048	0.286
MOD_GlcNHglycan	277	280	PF01048	0.244
MOD_GlcNHglycan	526	530	PF01048	0.679
MOD_GlcNHglycan	53	56	PF01048	0.481
MOD_GlcNHglycan	85	88	PF01048	0.492
MOD_GSK3_1	240	247	PF00069	0.410
MOD_GSK3_1	270	277	PF00069	0.493
MOD_GSK3_1	435	442	PF00069	0.452
MOD_GSK3_1	488	495	PF00069	0.617
MOD_GSK3_1	498	505	PF00069	0.668
MOD_GSK3_1	553	560	PF00069	0.739
MOD_N-GLC_1	493	498	PF02516	0.666
MOD_NEK2_1	177	182	PF00069	0.472
MOD_NEK2_1	244	249	PF00069	0.412
MOD_NEK2_1	270	275	PF00069	0.427
MOD_NEK2_1	371	376	PF00069	0.434
MOD_NEK2_1	441	446	PF00069	0.508
MOD_NEK2_1	456	461	PF00069	0.533
MOD_NEK2_1	60	65	PF00069	0.425
MOD_NEK2_1	95	100	PF00069	0.575
MOD_PIKK_1	171	177	PF00454	0.412
MOD_PIKK_1	270	276	PF00454	0.446
MOD_PIKK_1	456	462	PF00454	0.501
MOD_PK_1	222	228	PF00069	0.519
MOD_PKA_1	425	431	PF00069	0.444
MOD_PKA_1	584	590	PF00069	0.732
MOD_PKA_2	382	388	PF00069	0.381
MOD_PKA_2	481	487	PF00069	0.450
MOD_PKA_2	7	13	PF00069	0.771
MOD_PKB_1	584	592	PF00069	0.461
MOD_PKB_1	6	14	PF00069	0.448
MOD_Plk_1	240	246	PF00069	0.400
MOD_Plk_1	301	307	PF00069	0.496
MOD_Plk_1	330	336	PF00069	0.306
MOD_Plk_1	357	363	PF00069	0.403
MOD_Plk_1	388	394	PF00069	0.437
MOD_Plk_2-3	240	246	PF00069	0.410
MOD_Plk_4	282	288	PF00069	0.519
MOD_Plk_4	425	431	PF00069	0.549
MOD_ProDKin_1	159	165	PF00069	0.412
MOD_ProDKin_1	395	401	PF00069	0.455
MOD_ProDKin_1	538	544	PF00069	0.740
MOD_SUMO_rev_2	158	163	PF00179	0.519
MOD_SUMO_rev_2	446	454	PF00179	0.408
TRG_DiLeu_BaEn_1	347	352	PF01217	0.285
TRG_ENDOCYTIC_2	145	148	PF00928	0.306
TRG_ENDOCYTIC_2	154	157	PF00928	0.428
TRG_ENDOCYTIC_2	364	367	PF00928	0.405
TRG_ENDOCYTIC_2	470	473	PF00928	0.414
TRG_ER_diArg_1	141	144	PF00400	0.349
TRG_ER_diArg_1	5	8	PF00400	0.749
TRG_ER_diArg_1	99	101	PF00400	0.506
TRG_NES_CRM1_1	260	274	PF08389	0.519
TRG_NLS_MonoExtC_3	549	554	PF00514	0.571
TRG_NLS_MonoExtC_3	583	588	PF00514	0.470
TRG_NLS_MonoExtN_4	581	588	PF00514	0.501
TRG_Pf-PMV_PEXEL_1	248	252	PF00026	0.319
TRG_Pf-PMV_PEXEL_1	407	411	PF00026	0.292

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P7I6	Leptomonas seymouri	34%	85%
A0A0N1I6L1	Leptomonas seymouri	72%	90%
A0A0N1PDB9	Leptomonas seymouri	46%	96%
A0A0S4JDU0	Bodo saltans	38%	67%
A0A1X0NYM2	Trypanosomatidae	45%	98%
A0A1X0NZ35	Trypanosomatidae	50%	100%
A0A3Q8IEM0	Leishmania donovani	36%	79%
A0A3S7X1J7	Leishmania donovani	95%	100%
A4HGJ3	Leishmania braziliensis	37%	79%
A4I3M0	Leishmania infantum	36%	79%
A4I3M1	Leishmania infantum	95%	100%
A4I3M2	Leishmania infantum	39%	100%
D0A846	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	37%	100%
D0A847	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	40%	100%
D0A848	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	38%	100%
D0A849	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	38%	100%
D0A851	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	37%	100%
D0A852	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	37%	100%
E9AZW1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	36%	79%
E9AZW3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	44%	100%
O74944	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	32%	100%
Q4Q8C4	Leishmania major	94%	100%
Q4Q8C5	Leishmania major	35%	100%
Q6JDV7	Arabidopsis thaliana	36%	89%