DNA polymerase kappa

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DNA polymerase kappa, putative

Species:

Leishmania mexicana

UniProt:

E9AZW1_LEIMU

TriTrypDb:

LmxM.28.1410

Length:

753

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 14
NetGPI	no	yes: 0, no: 14

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9AZW1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AZW1

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	15
GO:0006259	DNA metabolic process	4	15
GO:0006281	DNA repair	5	15
GO:0006725	cellular aromatic compound metabolic process	3	15
GO:0006807	nitrogen compound metabolic process	2	15
GO:0006950	response to stress	2	15
GO:0006974	DNA damage response	4	15
GO:0008152	metabolic process	1	15
GO:0009987	cellular process	1	15
GO:0033554	cellular response to stress	3	15
GO:0034641	cellular nitrogen compound metabolic process	3	15
GO:0043170	macromolecule metabolic process	3	15
GO:0044237	cellular metabolic process	2	15
GO:0044238	primary metabolic process	2	15
GO:0044260	obsolete cellular macromolecule metabolic process	3	15
GO:0046483	heterocycle metabolic process	3	15
GO:0050896	response to stimulus	1	15
GO:0051716	cellular response to stimulus	2	15
GO:0071704	organic substance metabolic process	2	15
GO:0090304	nucleic acid metabolic process	4	15
GO:1901360	organic cyclic compound metabolic process	3	15
GO:0000731	DNA synthesis involved in DNA repair	6	2
GO:0006301	postreplication repair	6	2
GO:0009058	biosynthetic process	2	2
GO:0009059	macromolecule biosynthetic process	4	2
GO:0018130	heterocycle biosynthetic process	4	2
GO:0019438	aromatic compound biosynthetic process	4	2
GO:0019985	translesion synthesis	7	2
GO:0034654	nucleobase-containing compound biosynthetic process	4	2
GO:0042276	error-prone translesion synthesis	8	2
GO:0044249	cellular biosynthetic process	3	2
GO:0044271	cellular nitrogen compound biosynthetic process	4	2
GO:0071897	DNA biosynthetic process	5	2
GO:1901362	organic cyclic compound biosynthetic process	4	2
GO:1901576	organic substance biosynthetic process	3	2
GO:0006260	DNA replication	5	4

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	15
GO:0003677	DNA binding	4	15
GO:0003684	damaged DNA binding	5	15
GO:0003824	catalytic activity	1	15
GO:0003887	DNA-directed DNA polymerase activity	5	15
GO:0005488	binding	1	15
GO:0016740	transferase activity	2	15
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	15
GO:0016779	nucleotidyltransferase activity	4	15
GO:0034061	DNA polymerase activity	4	15
GO:0097159	organic cyclic compound binding	2	15
GO:0140097	catalytic activity, acting on DNA	3	15
GO:0140640	catalytic activity, acting on a nucleic acid	2	15
GO:1901363	heterocyclic compound binding	2	15
GO:0043167	ion binding	2	4
GO:0043169	cation binding	3	4
GO:0046872	metal ion binding	4	4
GO:0016491	oxidoreductase activity	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	129	133	PF00656	0.416
CLV_NRD_NRD_1	147	149	PF00675	0.362
CLV_NRD_NRD_1	178	180	PF00675	0.395
CLV_NRD_NRD_1	273	275	PF00675	0.284
CLV_NRD_NRD_1	31	33	PF00675	0.561
CLV_NRD_NRD_1	350	352	PF00675	0.181
CLV_NRD_NRD_1	364	366	PF00675	0.370
CLV_NRD_NRD_1	45	47	PF00675	0.510
CLV_NRD_NRD_1	454	456	PF00675	0.397
CLV_NRD_NRD_1	504	506	PF00675	0.264
CLV_NRD_NRD_1	532	534	PF00675	0.266
CLV_NRD_NRD_1	554	556	PF00675	0.188
CLV_NRD_NRD_1	577	579	PF00675	0.297
CLV_NRD_NRD_1	606	608	PF00675	0.331
CLV_NRD_NRD_1	662	664	PF00675	0.684
CLV_PCSK_KEX2_1	31	33	PF00082	0.564
CLV_PCSK_KEX2_1	350	352	PF00082	0.181
CLV_PCSK_KEX2_1	363	365	PF00082	0.364
CLV_PCSK_KEX2_1	532	534	PF00082	0.206
CLV_PCSK_KEX2_1	553	555	PF00082	0.162
CLV_PCSK_KEX2_1	577	579	PF00082	0.292
CLV_PCSK_KEX2_1	605	607	PF00082	0.340
CLV_PCSK_KEX2_1	664	666	PF00082	0.681
CLV_PCSK_PC1ET2_1	553	555	PF00082	0.162
CLV_PCSK_PC1ET2_1	664	666	PF00082	0.751
CLV_PCSK_SKI1_1	32	36	PF00082	0.532
CLV_PCSK_SKI1_1	577	581	PF00082	0.229
CLV_Separin_Metazoa	574	578	PF03568	0.429
DEG_APCC_DBOX_1	445	453	PF00400	0.315
DEG_APCC_DBOX_1	576	584	PF00400	0.429
DEG_Nend_UBRbox_3	1	3	PF02207	0.529
DEG_SCF_FBW7_1	58	65	PF00400	0.537
DEG_SPOP_SBC_1	478	482	PF00917	0.536
DOC_ANK_TNKS_1	666	673	PF00023	0.552
DOC_CKS1_1	59	64	PF01111	0.560
DOC_CYCLIN_yCln2_LP_2	517	523	PF00134	0.344
DOC_MAPK_gen_1	184	193	PF00069	0.389
DOC_MAPK_gen_1	577	585	PF00069	0.453
DOC_MAPK_HePTP_8	575	587	PF00069	0.421
DOC_MAPK_MEF2A_6	289	296	PF00069	0.436
DOC_MAPK_MEF2A_6	578	587	PF00069	0.421
DOC_MIT_MIM_1	594	606	PF04212	0.455
DOC_PP1_RVXF_1	139	146	PF00149	0.543
DOC_PP2B_LxvP_1	583	586	PF13499	0.430
DOC_SPAK_OSR1_1	351	355	PF12202	0.429
DOC_USP7_MATH_1	102	106	PF00917	0.745
DOC_USP7_MATH_1	107	111	PF00917	0.757
DOC_USP7_MATH_1	119	123	PF00917	0.604
DOC_USP7_MATH_1	479	483	PF00917	0.690
DOC_USP7_MATH_1	710	714	PF00917	0.650
DOC_USP7_MATH_1	745	749	PF00917	0.448
DOC_USP7_UBL2_3	165	169	PF12436	0.450
DOC_USP7_UBL2_3	560	564	PF12436	0.429
DOC_WW_Pin1_4	10	15	PF00397	0.738
DOC_WW_Pin1_4	378	383	PF00397	0.455
DOC_WW_Pin1_4	38	43	PF00397	0.679
DOC_WW_Pin1_4	46	51	PF00397	0.707
DOC_WW_Pin1_4	58	63	PF00397	0.531
DOC_WW_Pin1_4	70	75	PF00397	0.731
DOC_WW_Pin1_4	706	711	PF00397	0.580
DOC_WW_Pin1_4	715	720	PF00397	0.622
LIG_14-3-3_CanoR_1	115	119	PF00244	0.594
LIG_14-3-3_CanoR_1	225	230	PF00244	0.410
LIG_14-3-3_CanoR_1	32	42	PF00244	0.737
LIG_14-3-3_CanoR_1	455	459	PF00244	0.419
LIG_14-3-3_CanoR_1	505	509	PF00244	0.443
LIG_14-3-3_CanoR_1	532	538	PF00244	0.533
LIG_14-3-3_CanoR_1	554	558	PF00244	0.405
LIG_Actin_WH2_2	519	534	PF00022	0.531
LIG_APCC_ABBA_1	566	571	PF00400	0.497
LIG_BRCT_BRCA1_1	307	311	PF00533	0.497
LIG_BRCT_BRCA1_1	634	638	PF00533	0.393
LIG_Clathr_ClatBox_1	324	328	PF01394	0.406
LIG_FHA_1	158	164	PF00498	0.409
LIG_FHA_1	267	273	PF00498	0.497
LIG_FHA_1	379	385	PF00498	0.450
LIG_FHA_1	54	60	PF00498	0.538
LIG_FHA_1	616	622	PF00498	0.697
LIG_FHA_1	62	68	PF00498	0.562
LIG_FHA_1	637	643	PF00498	0.525
LIG_FHA_1	76	82	PF00498	0.595
LIG_FHA_2	34	40	PF00498	0.620
LIG_FHA_2	436	442	PF00498	0.295
LIG_FHA_2	535	541	PF00498	0.428
LIG_FHA_2	678	684	PF00498	0.496
LIG_FHA_2	700	706	PF00498	0.582
LIG_GSK3_LRP6_1	62	67	PF00069	0.521
LIG_LIR_Apic_2	389	394	PF02991	0.519
LIG_LIR_Gen_1	228	236	PF02991	0.334
LIG_LIR_Gen_1	239	247	PF02991	0.425
LIG_LIR_Gen_1	457	466	PF02991	0.385
LIG_LIR_Gen_1	590	599	PF02991	0.482
LIG_LIR_Nem_3	117	123	PF02991	0.417
LIG_LIR_Nem_3	207	212	PF02991	0.547
LIG_LIR_Nem_3	228	232	PF02991	0.337
LIG_LIR_Nem_3	239	245	PF02991	0.433
LIG_LIR_Nem_3	308	314	PF02991	0.468
LIG_LIR_Nem_3	457	461	PF02991	0.291
LIG_LIR_Nem_3	511	517	PF02991	0.450
LIG_LIR_Nem_3	590	594	PF02991	0.465
LIG_MLH1_MIPbox_1	634	638	PF16413	0.393
LIG_NRBOX	579	585	PF00104	0.497
LIG_PCNA_PIPBox_1	331	340	PF02747	0.497
LIG_PCNA_PIPBox_1	631	640	PF02747	0.520
LIG_PCNA_yPIPBox_3	179	192	PF02747	0.356
LIG_PCNA_yPIPBox_3	631	640	PF02747	0.482
LIG_PTAP_UEV_1	54	59	PF05743	0.472
LIG_PTB_Apo_2	247	254	PF02174	0.497
LIG_PTB_Phospho_1	247	253	PF10480	0.497
LIG_Rb_pABgroove_1	466	474	PF01858	0.455
LIG_REV1ctd_RIR_1	635	644	PF16727	0.553
LIG_SH2_CRK	120	124	PF00017	0.407
LIG_SH2_CRK	391	395	PF00017	0.397
LIG_SH2_CRK	528	532	PF00017	0.482
LIG_SH2_GRB2like	174	177	PF00017	0.373
LIG_SH2_NCK_1	253	257	PF00017	0.497
LIG_SH2_NCK_1	391	395	PF00017	0.290
LIG_SH2_SRC	253	256	PF00017	0.429
LIG_SH2_STAP1	198	202	PF00017	0.444
LIG_SH2_STAP1	458	462	PF00017	0.349
LIG_SH2_STAT3	276	279	PF00017	0.395
LIG_SH2_STAT5	144	147	PF00017	0.360
LIG_SH2_STAT5	174	177	PF00017	0.415
LIG_SH2_STAT5	271	274	PF00017	0.468
LIG_SH2_STAT5	337	340	PF00017	0.483
LIG_SH2_STAT5	411	414	PF00017	0.405
LIG_SH2_STAT5	451	454	PF00017	0.326
LIG_SH2_STAT5	569	572	PF00017	0.432
LIG_SH3_1	47	53	PF00018	0.482
LIG_SH3_2	42	47	PF14604	0.748
LIG_SH3_3	292	298	PF00018	0.448
LIG_SH3_3	39	45	PF00018	0.670
LIG_SH3_3	47	53	PF00018	0.687
LIG_SH3_3	56	62	PF00018	0.676
LIG_SH3_3	704	710	PF00018	0.694
LIG_SH3_4	393	400	PF00018	0.343
LIG_SUMO_SIM_par_1	293	300	PF11976	0.497
LIG_SUMO_SIM_par_1	617	624	PF11976	0.559
LIG_SUMO_SIM_par_1	691	699	PF11976	0.669
LIG_TYR_ITIM	118	123	PF00017	0.412
LIG_TYR_ITIM	526	531	PF00017	0.341
LIG_UBA3_1	163	169	PF00899	0.405
LIG_UBA3_1	449	456	PF00899	0.436
LIG_WRC_WIRS_1	123	128	PF05994	0.462
MOD_CDC14_SPxK_1	44	47	PF00782	0.470
MOD_CDK_SPK_2	41	46	PF00069	0.552
MOD_CDK_SPK_2	706	711	PF00069	0.483
MOD_CDK_SPxK_1	41	47	PF00069	0.475
MOD_CDK_SPxK_1	70	76	PF00069	0.538
MOD_CK1_1	101	107	PF00069	0.628
MOD_CK1_1	109	115	PF00069	0.648
MOD_CK1_1	122	128	PF00069	0.553
MOD_CK1_1	168	174	PF00069	0.433
MOD_CK1_1	201	207	PF00069	0.503
MOD_CK1_1	228	234	PF00069	0.416
MOD_CK1_1	264	270	PF00069	0.424
MOD_CK1_1	41	47	PF00069	0.730
MOD_CK1_1	426	432	PF00069	0.446
MOD_CK1_1	474	480	PF00069	0.584
MOD_CK1_1	534	540	PF00069	0.324
MOD_CK1_1	610	616	PF00069	0.558
MOD_CK1_1	696	702	PF00069	0.666
MOD_CK1_1	718	724	PF00069	0.606
MOD_CK1_1	8	14	PF00069	0.647
MOD_CK1_1	88	94	PF00069	0.718
MOD_CK2_1	435	441	PF00069	0.497
MOD_CK2_1	534	540	PF00069	0.290
MOD_CK2_1	620	626	PF00069	0.750
MOD_CK2_1	682	688	PF00069	0.677
MOD_CK2_1	699	705	PF00069	0.738
MOD_Cter_Amidation	503	506	PF01082	0.416
MOD_GlcNHglycan	104	107	PF01048	0.569
MOD_GlcNHglycan	109	112	PF01048	0.615
MOD_GlcNHglycan	153	156	PF01048	0.405
MOD_GlcNHglycan	279	282	PF01048	0.406
MOD_GlcNHglycan	307	310	PF01048	0.376
MOD_GlcNHglycan	374	377	PF01048	0.297
MOD_GlcNHglycan	481	484	PF01048	0.685
MOD_GlcNHglycan	7	10	PF01048	0.711
MOD_GlcNHglycan	720	723	PF01048	0.631
MOD_GlcNHglycan	726	729	PF01048	0.561
MOD_GlcNHglycan	742	746	PF01048	0.775
MOD_GlcNHglycan	99	103	PF01048	0.591
MOD_GSK3_1	106	113	PF00069	0.608
MOD_GSK3_1	122	129	PF00069	0.549
MOD_GSK3_1	13	20	PF00069	0.566
MOD_GSK3_1	131	138	PF00069	0.600
MOD_GSK3_1	165	172	PF00069	0.455
MOD_GSK3_1	261	268	PF00069	0.372
MOD_GSK3_1	4	11	PF00069	0.588
MOD_GSK3_1	474	481	PF00069	0.510
MOD_GSK3_1	483	490	PF00069	0.554
MOD_GSK3_1	58	65	PF00069	0.721
MOD_GSK3_1	610	617	PF00069	0.611
MOD_GSK3_1	626	633	PF00069	0.494
MOD_GSK3_1	641	648	PF00069	0.554
MOD_GSK3_1	66	73	PF00069	0.643
MOD_GSK3_1	706	713	PF00069	0.641
MOD_GSK3_1	718	725	PF00069	0.542
MOD_GSK3_1	741	748	PF00069	0.524
MOD_GSK3_1	85	92	PF00069	0.695
MOD_GSK3_1	98	105	PF00069	0.666
MOD_LATS_1	558	564	PF00433	0.363
MOD_N-GLC_1	169	174	PF02516	0.355
MOD_N-GLC_1	89	94	PF02516	0.686
MOD_NEK2_1	192	197	PF00069	0.491
MOD_NEK2_1	265	270	PF00069	0.297
MOD_NEK2_1	372	377	PF00069	0.281
MOD_NEK2_1	461	466	PF00069	0.302
MOD_NEK2_1	543	548	PF00069	0.299
MOD_NEK2_1	619	624	PF00069	0.542
MOD_NEK2_1	632	637	PF00069	0.420
MOD_NEK2_1	642	647	PF00069	0.495
MOD_NEK2_1	712	717	PF00069	0.687
MOD_NEK2_2	119	124	PF00069	0.458
MOD_NEK2_2	198	203	PF00069	0.349
MOD_NEK2_2	745	750	PF00069	0.449
MOD_PIKK_1	543	549	PF00454	0.348
MOD_PIKK_1	627	633	PF00454	0.508
MOD_PIKK_1	696	702	PF00454	0.666
MOD_PKA_1	553	559	PF00069	0.328
MOD_PKA_2	114	120	PF00069	0.711
MOD_PKA_2	426	432	PF00069	0.454
MOD_PKA_2	454	460	PF00069	0.421
MOD_PKA_2	504	510	PF00069	0.267
MOD_PKA_2	531	537	PF00069	0.268
MOD_PKA_2	553	559	PF00069	0.169
MOD_PKA_2	666	672	PF00069	0.582
MOD_PKA_2	677	683	PF00069	0.562
MOD_PKA_2	75	81	PF00069	0.658
MOD_PKB_1	605	613	PF00069	0.455
MOD_Plk_1	169	175	PF00069	0.357
MOD_Plk_1	198	204	PF00069	0.496
MOD_Plk_1	510	516	PF00069	0.297
MOD_Plk_1	627	633	PF00069	0.357
MOD_Plk_1	745	751	PF00069	0.504
MOD_Plk_2-3	17	23	PF00069	0.516
MOD_Plk_2-3	677	683	PF00069	0.513
MOD_Plk_4	122	128	PF00069	0.605
MOD_Plk_4	169	175	PF00069	0.462
MOD_Plk_4	228	234	PF00069	0.433
MOD_Plk_4	24	30	PF00069	0.599
MOD_Plk_4	261	267	PF00069	0.236
MOD_Plk_4	426	432	PF00069	0.438
MOD_Plk_4	745	751	PF00069	0.450
MOD_ProDKin_1	10	16	PF00069	0.738
MOD_ProDKin_1	378	384	PF00069	0.305
MOD_ProDKin_1	38	44	PF00069	0.679
MOD_ProDKin_1	46	52	PF00069	0.705
MOD_ProDKin_1	58	64	PF00069	0.532
MOD_ProDKin_1	70	76	PF00069	0.733
MOD_ProDKin_1	706	712	PF00069	0.581
MOD_ProDKin_1	715	721	PF00069	0.621
MOD_SUMO_for_1	158	161	PF00179	0.292
MOD_SUMO_rev_2	146	150	PF00179	0.535
MOD_SUMO_rev_2	172	181	PF00179	0.489
MOD_SUMO_rev_2	703	710	PF00179	0.565
TRG_DiLeu_BaEn_1	21	26	PF01217	0.559
TRG_DiLeu_BaLyEn_6	592	597	PF01217	0.291
TRG_ENDOCYTIC_2	120	123	PF00928	0.405
TRG_ENDOCYTIC_2	233	236	PF00928	0.291
TRG_ENDOCYTIC_2	253	256	PF00928	0.305
TRG_ENDOCYTIC_2	458	461	PF00928	0.410
TRG_ENDOCYTIC_2	472	475	PF00928	0.587
TRG_ENDOCYTIC_2	528	531	PF00928	0.255
TRG_ER_diArg_1	350	352	PF00400	0.206
TRG_ER_diArg_1	363	365	PF00400	0.246
TRG_ER_diArg_1	446	449	PF00400	0.313
TRG_ER_diArg_1	531	533	PF00400	0.382
TRG_ER_diArg_1	576	578	PF00400	0.339
TRG_ER_diArg_1	604	607	PF00400	0.442
TRG_ER_diArg_1	662	665	PF00400	0.705
TRG_NES_CRM1_1	323	336	PF08389	0.365
TRG_NLS_MonoCore_2	663	668	PF00514	0.554
TRG_NLS_MonoExtC_3	662	667	PF00514	0.691
TRG_NLS_MonoExtN_4	551	557	PF00514	0.148
TRG_NLS_MonoExtN_4	663	668	PF00514	0.563
TRG_Pf-PMV_PEXEL_1	210	215	PF00026	0.362
TRG_Pf-PMV_PEXEL_1	357	361	PF00026	0.268
TRG_Pf-PMV_PEXEL_1	364	368	PF00026	0.221
TRG_Pf-PMV_PEXEL_1	437	441	PF00026	0.246
TRG_Pf-PMV_PEXEL_1	578	582	PF00026	0.363

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P7I6	Leptomonas seymouri	59%	100%
A0A0N1PDB9	Leptomonas seymouri	34%	100%
A0A0S4JDU0	Bodo saltans	37%	85%
A0A1X0NYM2	Trypanosomatidae	46%	100%
A0A3Q8IEM0	Leishmania donovani	88%	100%
A0A3S7X1J7	Leishmania donovani	37%	100%
A4HGJ3	Leishmania braziliensis	71%	99%
A4I3M0	Leishmania infantum	88%	100%
A4I3M1	Leishmania infantum	37%	100%
D0A848	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	37%	100%
E9AZW2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	36%	100%
P34409	Caenorhabditis elegans	31%	100%
Q4Q8C4	Leishmania major	36%	100%
Q4Q8C5	Leishmania major	87%	100%