Tubulin-tyrosine ligase-like protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tubulin-tyrosine ligase-like protein

Species:

Leishmania mexicana

UniProt:

E9AZF8_LEIMU

TriTrypDb:

LmxM.27.2490

Length:

476

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005874	microtubule	6	1
GO:0005876	spindle microtubule	7	1
GO:0099080	supramolecular complex	2	1
GO:0099081	supramolecular polymer	3	1
GO:0099512	supramolecular fiber	4	1
GO:0099513	polymeric cytoskeletal fiber	5	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AZF8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AZF8

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0019538	protein metabolic process	3	12
GO:0036211	protein modification process	4	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0044238	primary metabolic process	2	12
GO:0071704	organic substance metabolic process	2	12
GO:1901564	organonitrogen compound metabolic process	3	12
GO:0000226	microtubule cytoskeleton organization	3	1
GO:0006996	organelle organization	4	1
GO:0007010	cytoskeleton organization	5	1
GO:0007017	microtubule-based process	2	1
GO:0009987	cellular process	1	1
GO:0016043	cellular component organization	3	1
GO:0071840	cellular component organization or biogenesis	2	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0016874	ligase activity	2	12
GO:0004835	tubulin-tyrosine ligase activity	3	2
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	2
GO:0016881	acid-amino acid ligase activity	4	2
GO:0140096	catalytic activity, acting on a protein	2	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	154	156	PF00675	0.294
CLV_NRD_NRD_1	182	184	PF00675	0.148
CLV_NRD_NRD_1	222	224	PF00675	0.274
CLV_NRD_NRD_1	28	30	PF00675	0.676
CLV_NRD_NRD_1	432	434	PF00675	0.767
CLV_NRD_NRD_1	65	67	PF00675	0.534
CLV_PCSK_KEX2_1	154	156	PF00082	0.266
CLV_PCSK_KEX2_1	182	184	PF00082	0.247
CLV_PCSK_KEX2_1	431	433	PF00082	0.689
CLV_PCSK_KEX2_1	65	67	PF00082	0.541
CLV_PCSK_SKI1_1	154	158	PF00082	0.251
CLV_PCSK_SKI1_1	182	186	PF00082	0.291
CLV_PCSK_SKI1_1	215	219	PF00082	0.265
CLV_PCSK_SKI1_1	315	319	PF00082	0.287
CLV_PCSK_SKI1_1	320	324	PF00082	0.275
CLV_PCSK_SKI1_1	369	373	PF00082	0.476
CLV_PCSK_SKI1_1	379	383	PF00082	0.474
CLV_PCSK_SKI1_1	94	98	PF00082	0.477
DEG_APCC_DBOX_1	154	162	PF00400	0.537
DEG_APCC_DBOX_1	319	327	PF00400	0.504
DEG_SPOP_SBC_1	3	7	PF00917	0.527
DEG_SPOP_SBC_1	421	425	PF00917	0.559
DOC_CKS1_1	370	375	PF01111	0.477
DOC_CYCLIN_RxL_1	309	321	PF00134	0.543
DOC_CYCLIN_yCln2_LP_2	233	239	PF00134	0.451
DOC_MAPK_gen_1	180	187	PF00069	0.493
DOC_MAPK_gen_1	223	229	PF00069	0.453
DOC_PP4_FxxP_1	24	27	PF00568	0.435
DOC_PP4_FxxP_1	370	373	PF00568	0.448
DOC_PP4_FxxP_1	83	86	PF00568	0.379
DOC_SPAK_OSR1_1	82	86	PF12202	0.374
DOC_USP7_MATH_1	18	22	PF00917	0.611
DOC_USP7_MATH_1	200	204	PF00917	0.500
DOC_USP7_MATH_1	25	29	PF00917	0.660
DOC_USP7_MATH_1	3	7	PF00917	0.621
DOC_USP7_MATH_1	31	35	PF00917	0.646
DOC_USP7_MATH_1	396	400	PF00917	0.741
DOC_USP7_MATH_1	435	439	PF00917	0.684
DOC_USP7_MATH_1	96	100	PF00917	0.424
DOC_WW_Pin1_4	232	237	PF00397	0.462
DOC_WW_Pin1_4	360	365	PF00397	0.441
DOC_WW_Pin1_4	369	374	PF00397	0.369
DOC_WW_Pin1_4	417	422	PF00397	0.743
DOC_WW_Pin1_4	431	436	PF00397	0.708
DOC_WW_Pin1_4	439	444	PF00397	0.697
DOC_WW_Pin1_4	7	12	PF00397	0.635
LIG_14-3-3_CanoR_1	115	119	PF00244	0.463
LIG_14-3-3_CanoR_1	188	196	PF00244	0.556
LIG_14-3-3_CanoR_1	248	253	PF00244	0.451
LIG_14-3-3_CanoR_1	436	443	PF00244	0.707
LIG_14-3-3_CanoR_1	65	72	PF00244	0.465
LIG_APCC_ABBA_1	49	54	PF00400	0.363
LIG_BIR_III_2	466	470	PF00653	0.474
LIG_BRCT_BRCA1_1	20	24	PF00533	0.710
LIG_BRCT_BRCA1_1	45	49	PF00533	0.498
LIG_BRCT_BRCA1_1	97	101	PF00533	0.498
LIG_Clathr_ClatBox_1	89	93	PF01394	0.445
LIG_CSL_BTD_1	135	138	PF09270	0.473
LIG_CSL_BTD_1	287	290	PF09270	0.476
LIG_deltaCOP1_diTrp_1	130	136	PF00928	0.487
LIG_eIF4E_1	212	218	PF01652	0.451
LIG_eIF4E_1	337	343	PF01652	0.476
LIG_FHA_1	102	108	PF00498	0.427
LIG_FHA_1	247	253	PF00498	0.417
LIG_FHA_1	306	312	PF00498	0.526
LIG_FHA_1	370	376	PF00498	0.415
LIG_FHA_1	442	448	PF00498	0.485
LIG_FHA_1	46	52	PF00498	0.484
LIG_FHA_2	400	406	PF00498	0.712
LIG_GBD_Chelix_1	318	326	PF00786	0.343
LIG_Integrin_isoDGR_2	221	223	PF01839	0.300
LIG_Integrin_RGD_1	464	466	PF01839	0.487
LIG_LIR_Apic_2	21	27	PF02991	0.437
LIG_LIR_Apic_2	368	373	PF02991	0.392
LIG_LIR_Apic_2	81	86	PF02991	0.368
LIG_LIR_Gen_1	50	59	PF02991	0.357
LIG_LIR_Nem_3	104	109	PF02991	0.446
LIG_LIR_Nem_3	235	240	PF02991	0.462
LIG_LIR_Nem_3	334	340	PF02991	0.451
LIG_LIR_Nem_3	455	461	PF02991	0.493
LIG_LIR_Nem_3	46	52	PF02991	0.388
LIG_PCNA_PIPBox_1	126	135	PF02747	0.500
LIG_Pex14_1	106	110	PF04695	0.338
LIG_Pex14_2	458	462	PF04695	0.374
LIG_SH2_CRK	345	349	PF00017	0.451
LIG_SH2_CRK	87	91	PF00017	0.377
LIG_SH2_NCK_1	242	246	PF00017	0.537
LIG_SH2_SRC	141	144	PF00017	0.526
LIG_SH2_STAP1	237	241	PF00017	0.451
LIG_SH2_STAP1	345	349	PF00017	0.451
LIG_SH2_STAP1	52	56	PF00017	0.385
LIG_SH2_STAT3	207	210	PF00017	0.512
LIG_SH2_STAT5	109	112	PF00017	0.452
LIG_SH2_STAT5	207	210	PF00017	0.462
LIG_SH2_STAT5	212	215	PF00017	0.438
LIG_SH2_STAT5	242	245	PF00017	0.520
LIG_SH2_STAT5	295	298	PF00017	0.462
LIG_SH2_STAT5	42	45	PF00017	0.450
LIG_SH2_STAT5	87	90	PF00017	0.521
LIG_SH3_3	210	216	PF00018	0.451
LIG_SUMO_SIM_par_1	214	220	PF11976	0.537
LIG_TYR_ITIM	85	90	PF00017	0.355
MOD_CDK_SPK_2	431	436	PF00069	0.473
MOD_CDK_SPxxK_3	369	376	PF00069	0.451
MOD_CK1_1	20	26	PF00069	0.695
MOD_CK1_1	235	241	PF00069	0.472
MOD_CK1_1	246	252	PF00069	0.420
MOD_CK1_1	294	300	PF00069	0.500
MOD_CK1_1	38	44	PF00069	0.278
MOD_CK1_1	392	398	PF00069	0.711
MOD_CK1_1	399	405	PF00069	0.739
MOD_CK1_1	413	419	PF00069	0.631
MOD_CK1_1	420	426	PF00069	0.743
MOD_CK1_1	439	445	PF00069	0.646
MOD_CK1_1	47	53	PF00069	0.372
MOD_CK2_1	374	380	PF00069	0.499
MOD_CK2_1	399	405	PF00069	0.710
MOD_CK2_1	47	53	PF00069	0.399
MOD_CMANNOS	133	136	PF00535	0.337
MOD_Cter_Amidation	180	183	PF01082	0.148
MOD_Cter_Amidation	221	224	PF01082	0.263
MOD_Cter_Amidation	429	432	PF01082	0.684
MOD_GlcNHglycan	176	179	PF01048	0.256
MOD_GlcNHglycan	189	192	PF01048	0.251
MOD_GlcNHglycan	33	36	PF01048	0.655
MOD_GlcNHglycan	37	40	PF01048	0.486
MOD_GlcNHglycan	415	418	PF01048	0.730
MOD_GlcNHglycan	428	431	PF01048	0.668
MOD_GlcNHglycan	438	441	PF01048	0.692
MOD_GSK3_1	110	117	PF00069	0.447
MOD_GSK3_1	128	135	PF00069	0.473
MOD_GSK3_1	291	298	PF00069	0.500
MOD_GSK3_1	3	10	PF00069	0.671
MOD_GSK3_1	31	38	PF00069	0.587
MOD_GSK3_1	387	394	PF00069	0.673
MOD_GSK3_1	396	403	PF00069	0.650
MOD_GSK3_1	405	412	PF00069	0.560
MOD_GSK3_1	413	420	PF00069	0.742
MOD_GSK3_1	422	429	PF00069	0.464
MOD_GSK3_1	43	50	PF00069	0.528
MOD_GSK3_1	431	438	PF00069	0.523
MOD_GSK3_1	439	446	PF00069	0.616
MOD_N-GLC_1	360	365	PF02516	0.251
MOD_N-GLC_1	409	414	PF02516	0.693
MOD_N-GLC_2	268	270	PF02516	0.251
MOD_NEK2_1	101	106	PF00069	0.357
MOD_NEK2_1	110	115	PF00069	0.384
MOD_NEK2_1	187	192	PF00069	0.497
MOD_NEK2_1	217	222	PF00069	0.566
MOD_NEK2_1	305	310	PF00069	0.538
MOD_NEK2_1	318	323	PF00069	0.532
MOD_NEK2_1	374	379	PF00069	0.482
MOD_NEK2_1	4	9	PF00069	0.625
MOD_NEK2_1	43	48	PF00069	0.387
MOD_NEK2_1	95	100	PF00069	0.444
MOD_NEK2_2	202	207	PF00069	0.431
MOD_PIKK_1	240	246	PF00454	0.512
MOD_PK_1	248	254	PF00069	0.429
MOD_PKA_1	65	71	PF00069	0.486
MOD_PKA_2	114	120	PF00069	0.463
MOD_PKA_2	187	193	PF00069	0.534
MOD_PKA_2	31	37	PF00069	0.649
MOD_PKA_2	435	441	PF00069	0.718
MOD_PKA_2	65	71	PF00069	0.436
MOD_Plk_4	132	138	PF00069	0.476
MOD_Plk_4	235	241	PF00069	0.480
MOD_Plk_4	291	297	PF00069	0.500
MOD_Plk_4	38	44	PF00069	0.423
MOD_Plk_4	85	91	PF00069	0.370
MOD_Plk_4	96	102	PF00069	0.395
MOD_ProDKin_1	232	238	PF00069	0.462
MOD_ProDKin_1	360	366	PF00069	0.441
MOD_ProDKin_1	369	375	PF00069	0.363
MOD_ProDKin_1	417	423	PF00069	0.741
MOD_ProDKin_1	431	437	PF00069	0.707
MOD_ProDKin_1	439	445	PF00069	0.697
MOD_ProDKin_1	7	13	PF00069	0.636
TRG_DiLeu_BaEn_1	153	158	PF01217	0.537
TRG_DiLeu_BaEn_1	456	461	PF01217	0.521
TRG_DiLeu_BaEn_2	453	459	PF01217	0.540
TRG_DiLeu_BaLyEn_6	213	218	PF01217	0.451
TRG_DiLeu_BaLyEn_6	353	358	PF01217	0.554
TRG_ENDOCYTIC_2	337	340	PF00928	0.455
TRG_ENDOCYTIC_2	345	348	PF00928	0.444
TRG_ENDOCYTIC_2	52	55	PF00928	0.353
TRG_ENDOCYTIC_2	87	90	PF00928	0.358
TRG_ER_diArg_1	182	184	PF00400	0.348
TRG_ER_diArg_1	431	433	PF00400	0.689
TRG_ER_diArg_1	64	66	PF00400	0.554
TRG_NLS_MonoExtN_4	27	33	PF00514	0.585
TRG_Pf-PMV_PEXEL_1	155	159	PF00026	0.324
TRG_Pf-PMV_PEXEL_1	57	61	PF00026	0.449

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P402	Leptomonas seymouri	23%	100%
A0A0N1I1A1	Leptomonas seymouri	66%	100%
A0A0S4ISF0	Bodo saltans	25%	84%
A0A0S4J6I6	Bodo saltans	41%	100%
A0A1X0NFL2	Trypanosomatidae	52%	100%
A0A1X0NRN8	Trypanosomatidae	32%	96%
A0A1X0P6A0	Trypanosomatidae	25%	71%
A0A3Q8IDP1	Leishmania donovani	94%	89%
A0A422NE52	Trypanosoma rangeli	51%	100%
A4H9E1	Leishmania braziliensis	23%	100%
A4HG29	Leishmania braziliensis	78%	100%
A4HXR2	Leishmania infantum	22%	100%
A4I351	Leishmania infantum	94%	100%
A4Q9E4	Mus musculus	26%	88%
C9ZJM2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	52%	100%
E9ACW9	Leishmania major	92%	100%
E9AM52	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	80%
E9ARH4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	22%	100%
P38160	Sus scrofa	34%	100%
P38584	Bos taurus	34%	100%
P38585	Mus musculus	34%	100%
Q23SI8	Tetrahymena thermophila (strain SB210)	25%	100%
Q4QE05	Leishmania major	22%	100%
Q8NG68	Homo sapiens	34%	100%
Q9BWV7	Homo sapiens	27%	80%
Q9QXJ0	Rattus norvegicus	34%	100%
V5B4U2	Trypanosoma cruzi	23%	86%
V5D711	Trypanosoma cruzi	52%	100%