Orange domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

EMG1/NEP1 methyltransferase, putative

Species:

Leishmania mexicana

UniProt:

E9AZD1_LEIMU

TriTrypDb:

LmxM.27.2250

Length:

246

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005730	nucleolus	5	1
GO:0030684	preribosome	3	1
GO:0032040	small-subunit processome	4	1
GO:0032991	protein-containing complex	1	1
GO:0043226	organelle	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1
GO:1990904	ribonucleoprotein complex	2	1

Expansion

Sequence features

E9AZD1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AZD1

Function

Biological processes
Term	Name	Level	Count
GO:0000154	rRNA modification	6	12
GO:0001510	RNA methylation	4	12
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006364	rRNA processing	8	12
GO:0006396	RNA processing	6	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009451	RNA modification	5	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0016072	rRNA metabolic process	7	12
GO:0031167	rRNA methylation	5	12
GO:0032259	methylation	2	12
GO:0034470	ncRNA processing	7	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034660	ncRNA metabolic process	6	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0043414	macromolecule methylation	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0046483	heterocycle metabolic process	3	12
GO:0070475	rRNA base methylation	6	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0008168	methyltransferase activity	4	12
GO:0008173	RNA methyltransferase activity	4	12
GO:0008649	rRNA methyltransferase activity	5	12
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0019843	rRNA binding	5	12
GO:0070037	rRNA (pseudouridine) methyltransferase activity	6	12
GO:0097159	organic cyclic compound binding	2	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140102	catalytic activity, acting on a rRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	231	233	PF00675	0.632
CLV_PCSK_KEX2_1	230	232	PF00082	0.764
CLV_PCSK_SKI1_1	149	153	PF00082	0.339
CLV_PCSK_SKI1_1	9	13	PF00082	0.382
CLV_Separin_Metazoa	227	231	PF03568	0.465
DOC_MAPK_gen_1	43	52	PF00069	0.249
DOC_MAPK_HePTP_8	40	52	PF00069	0.145
DOC_MAPK_MEF2A_6	43	52	PF00069	0.249
DOC_MAPK_NFAT4_5	47	55	PF00069	0.299
DOC_PP1_RVXF_1	74	81	PF00149	0.145
DOC_PP2B_LxvP_1	66	69	PF13499	0.236
DOC_USP7_MATH_1	16	20	PF00917	0.251
DOC_USP7_MATH_1	200	204	PF00917	0.557
DOC_WW_Pin1_4	120	125	PF00397	0.379
DOC_WW_Pin1_4	222	227	PF00397	0.559
DOC_WW_Pin1_4	60	65	PF00397	0.251
LIG_14-3-3_CanoR_1	222	226	PF00244	0.632
LIG_14-3-3_CanoR_1	47	53	PF00244	0.258
LIG_14-3-3_CanoR_1	67	73	PF00244	0.117
LIG_14-3-3_CanoR_1	87	97	PF00244	0.117
LIG_Actin_WH2_2	71	86	PF00022	0.251
LIG_FHA_1	10	16	PF00498	0.382
LIG_FHA_1	116	122	PF00498	0.274
LIG_FHA_1	170	176	PF00498	0.292
LIG_FHA_1	181	187	PF00498	0.298
LIG_FHA_2	194	200	PF00498	0.477
LIG_LIR_Gen_1	71	80	PF02991	0.382
LIG_LIR_Nem_3	152	157	PF02991	0.406
LIG_LIR_Nem_3	71	75	PF02991	0.251
LIG_MYND_1	64	68	PF01753	0.236
LIG_Rb_pABgroove_1	30	38	PF01858	0.236
LIG_SH2_NCK_1	7	11	PF00017	0.236
LIG_SH2_PTP2	49	52	PF00017	0.256
LIG_SH2_STAP1	7	11	PF00017	0.270
LIG_SH2_STAT3	170	173	PF00017	0.154
LIG_SH2_STAT5	155	158	PF00017	0.310
LIG_SH2_STAT5	170	173	PF00017	0.262
LIG_SH2_STAT5	49	52	PF00017	0.238
LIG_SH3_2	226	231	PF14604	0.578
LIG_SH3_3	103	109	PF00018	0.236
LIG_SH3_3	133	139	PF00018	0.367
LIG_SH3_3	223	229	PF00018	0.581
LIG_SUMO_SIM_par_1	31	39	PF11976	0.236
LIG_SUMO_SIM_par_1	58	63	PF11976	0.251
LIG_WW_3	227	231	PF00397	0.465
LIG_WW_3	67	71	PF00397	0.236
MOD_CDK_SPxxK_3	60	67	PF00069	0.251
MOD_CK1_1	120	126	PF00069	0.456
MOD_CK1_1	202	208	PF00069	0.677
MOD_CK2_1	193	199	PF00069	0.451
MOD_CK2_1	221	227	PF00069	0.632
MOD_DYRK1A_RPxSP_1	222	226	PF00069	0.478
MOD_GlcNHglycan	177	180	PF01048	0.276
MOD_GlcNHglycan	204	207	PF01048	0.681
MOD_GSK3_1	115	122	PF00069	0.295
MOD_GSK3_1	171	178	PF00069	0.432
MOD_GSK3_1	200	207	PF00069	0.605
MOD_GSK3_1	217	224	PF00069	0.743
MOD_N-GLC_1	16	21	PF02516	0.240
MOD_NEK2_1	117	122	PF00069	0.340
MOD_NEK2_2	16	21	PF00069	0.295
MOD_NMyristoyl	1	7	PF02799	0.299
MOD_PIKK_1	126	132	PF00454	0.251
MOD_PIKK_1	169	175	PF00454	0.154
MOD_PKA_2	221	227	PF00069	0.642
MOD_Plk_1	140	146	PF00069	0.406
MOD_Plk_1	16	22	PF00069	0.240
MOD_ProDKin_1	120	126	PF00069	0.379
MOD_ProDKin_1	222	228	PF00069	0.556
MOD_ProDKin_1	60	66	PF00069	0.251
TRG_ENDOCYTIC_2	49	52	PF00928	0.270
TRG_ENDOCYTIC_2	72	75	PF00928	0.330
TRG_ER_diArg_1	229	232	PF00400	0.475
TRG_ER_diArg_1	42	45	PF00400	0.248

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8T8	Leptomonas seymouri	91%	69%
A0A0S4IQP7	Bodo saltans	69%	72%
A0A1X0NR30	Trypanosomatidae	69%	91%
A0A3Q8IDJ6	Leishmania donovani	98%	100%
A0A422NAI1	Trypanosoma rangeli	70%	84%
A4HG02	Leishmania braziliensis	92%	100%
A4I321	Leishmania infantum	98%	100%
C9ZJJ4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	65%	88%
E9ACU2	Leishmania major	95%	100%
O35130	Mus musculus	31%	100%
Q06287	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	30%	98%
Q10107	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	30%	69%
Q92979	Homo sapiens	33%	100%
Q96UP2	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	29%	100%
Q9P8P7	Candida albicans	29%	92%
Q9W4J5	Drosophila melanogaster	28%	98%
Q9XX15	Caenorhabditis elegans	35%	100%
V5B3U5	Trypanosoma cruzi	69%	89%