Putative trypanothione synthetase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

trypanothione synthetase

Species:

Leishmania mexicana

UniProt:

E9AZ89_LEIMU

TriTrypDb:

LmxM.27.1870

Length:

652

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 2
Forrest at al. (procyclic)	no	yes: 2
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 9

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 5

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 5

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 5
NetGPI	no	yes: 0, no: 5

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AZ89

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AZ89

PDB structure(s): 2vob_A , 2vob_B , 2vpm_A , 2vpm_B , 2vps_A

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	6
GO:0016874	ligase activity	2	6
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	6
GO:0016880	acid-ammonia (or amide) ligase activity	4	6
GO:0047479	trypanothione synthase activity	5	6

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	175	179	PF00656	0.399
CLV_C14_Caspase3-7	561	565	PF00656	0.441
CLV_C14_Caspase3-7	620	624	PF00656	0.377
CLV_C14_Caspase3-7	89	93	PF00656	0.352
CLV_MEL_PAP_1	550	556	PF00089	0.377
CLV_NRD_NRD_1	222	224	PF00675	0.531
CLV_NRD_NRD_1	540	542	PF00675	0.441
CLV_NRD_NRD_1	64	66	PF00675	0.598
CLV_NRD_NRD_1	69	71	PF00675	0.541
CLV_PCSK_KEX2_1	222	224	PF00082	0.531
CLV_PCSK_KEX2_1	386	388	PF00082	0.202
CLV_PCSK_KEX2_1	64	66	PF00082	0.598
CLV_PCSK_PC1ET2_1	386	388	PF00082	0.202
CLV_PCSK_SKI1_1	383	387	PF00082	0.441
CLV_PCSK_SKI1_1	513	517	PF00082	0.441
CLV_PCSK_SKI1_1	65	69	PF00082	0.596
DEG_Kelch_Keap1_1	334	339	PF01344	0.377
DOC_CYCLIN_RxL_1	65	77	PF00134	0.318
DOC_CYCLIN_yCln2_LP_2	636	642	PF00134	0.578
DOC_MAPK_gen_1	485	493	PF00069	0.441
DOC_MAPK_MEF2A_6	197	206	PF00069	0.585
DOC_PP1_RVXF_1	395	401	PF00149	0.441
DOC_PP2B_LxvP_1	73	76	PF13499	0.364
DOC_PP4_FxxP_1	11	14	PF00568	0.470
DOC_PP4_FxxP_1	212	215	PF00568	0.551
DOC_PP4_FxxP_1	304	307	PF00568	0.441
DOC_USP7_MATH_1	227	231	PF00917	0.536
DOC_USP7_MATH_1	7	11	PF00917	0.446
DOC_USP7_UBL2_3	479	483	PF12436	0.441
DOC_USP7_UBL2_3	643	647	PF12436	0.490
DOC_WW_Pin1_4	478	483	PF00397	0.441
DOC_WW_Pin1_4	621	626	PF00397	0.447
LIG_14-3-3_CanoR_1	313	319	PF00244	0.498
LIG_14-3-3_CanoR_1	6	12	PF00244	0.460
LIG_BIR_II_1	1	5	PF00653	0.565
LIG_BRCT_BRCA1_1	208	212	PF00533	0.553
LIG_BRCT_BRCA1_1	325	329	PF00533	0.441
LIG_BRCT_BRCA1_1	7	11	PF00533	0.455
LIG_deltaCOP1_diTrp_1	308	318	PF00928	0.441
LIG_deltaCOP1_diTrp_1	495	502	PF00928	0.441
LIG_deltaCOP1_diTrp_1	77	85	PF00928	0.318
LIG_FHA_1	123	129	PF00498	0.286
LIG_FHA_1	289	295	PF00498	0.466
LIG_FHA_1	335	341	PF00498	0.441
LIG_FHA_1	393	399	PF00498	0.441
LIG_FHA_1	535	541	PF00498	0.377
LIG_FHA_2	170	176	PF00498	0.488
LIG_FHA_2	254	260	PF00498	0.569
LIG_FHA_2	350	356	PF00498	0.451
LIG_FHA_2	559	565	PF00498	0.441
LIG_FHA_2	618	624	PF00498	0.441
LIG_FHA_2	639	645	PF00498	0.515
LIG_LIR_Apic_2	209	215	PF02991	0.550
LIG_LIR_Apic_2	498	504	PF02991	0.441
LIG_LIR_Apic_2	8	14	PF02991	0.463
LIG_LIR_Gen_1	139	149	PF02991	0.364
LIG_LIR_Gen_1	168	179	PF02991	0.509
LIG_LIR_Gen_1	407	418	PF02991	0.441
LIG_LIR_Gen_1	495	505	PF02991	0.441
LIG_LIR_Gen_1	592	603	PF02991	0.441
LIG_LIR_Gen_1	623	633	PF02991	0.441
LIG_LIR_Gen_1	97	104	PF02991	0.368
LIG_LIR_Nem_3	139	144	PF02991	0.364
LIG_LIR_Nem_3	168	174	PF02991	0.550
LIG_LIR_Nem_3	308	314	PF02991	0.441
LIG_LIR_Nem_3	407	413	PF02991	0.441
LIG_LIR_Nem_3	49	55	PF02991	0.329
LIG_LIR_Nem_3	495	500	PF02991	0.441
LIG_LIR_Nem_3	503	509	PF02991	0.333
LIG_LIR_Nem_3	592	598	PF02991	0.441
LIG_LIR_Nem_3	623	629	PF02991	0.441
LIG_LIR_Nem_3	97	102	PF02991	0.368
LIG_NRBOX	487	493	PF00104	0.441
LIG_Pex14_1	154	158	PF04695	0.457
LIG_Pex14_1	497	501	PF04695	0.441
LIG_Pex14_2	245	249	PF04695	0.527
LIG_Pex14_2	329	333	PF04695	0.441
LIG_Pex14_2	502	506	PF04695	0.441
LIG_Pex14_2	62	66	PF04695	0.339
LIG_Rb_pABgroove_1	426	434	PF01858	0.441
LIG_REV1ctd_RIR_1	50	60	PF16727	0.377
LIG_SH2_CRK	410	414	PF00017	0.441
LIG_SH2_CRK	582	586	PF00017	0.441
LIG_SH2_CRK	595	599	PF00017	0.265
LIG_SH2_GRB2like	33	36	PF00017	0.496
LIG_SH2_GRB2like	520	523	PF00017	0.441
LIG_SH2_NCK_1	595	599	PF00017	0.441
LIG_SH2_PTP2	141	144	PF00017	0.364
LIG_SH2_SRC	25	28	PF00017	0.513
LIG_SH2_SRC	595	598	PF00017	0.441
LIG_SH2_STAP1	158	162	PF00017	0.441
LIG_SH2_STAP1	410	414	PF00017	0.441
LIG_SH2_STAT3	279	282	PF00017	0.441
LIG_SH2_STAT3	414	417	PF00017	0.441
LIG_SH2_STAT5	141	144	PF00017	0.364
LIG_SH2_STAT5	219	222	PF00017	0.476
LIG_SH2_STAT5	324	327	PF00017	0.505
LIG_SH2_STAT5	40	43	PF00017	0.488
LIG_SH2_STAT5	410	413	PF00017	0.441
LIG_SH2_STAT5	414	417	PF00017	0.387
LIG_SH2_STAT5	501	504	PF00017	0.441
LIG_SH3_3	186	192	PF00018	0.425
LIG_SH3_3	212	218	PF00018	0.542
LIG_SH3_3	521	527	PF00018	0.505
LIG_SH3_3	619	625	PF00018	0.441
LIG_SH3_4	479	486	PF00018	0.441
LIG_TRAF2_1	470	473	PF00917	0.441
LIG_TYR_ITAM	579	598	PF00017	0.441
LIG_UBA3_1	585	590	PF00899	0.441
MOD_CDK_SPK_2	478	483	PF00069	0.441
MOD_CDK_SPxxK_3	478	485	PF00069	0.441
MOD_CK1_1	122	128	PF00069	0.284
MOD_CK1_1	349	355	PF00069	0.457
MOD_CK1_1	392	398	PF00069	0.505
MOD_CK1_1	462	468	PF00069	0.444
MOD_CK1_1	556	562	PF00069	0.390
MOD_CK1_1	617	623	PF00069	0.466
MOD_CK2_1	253	259	PF00069	0.627
MOD_CK2_1	261	267	PF00069	0.383
MOD_CK2_1	302	308	PF00069	0.202
MOD_CK2_1	349	355	PF00069	0.441
MOD_CMANNOS	455	458	PF00535	0.441
MOD_GlcNHglycan	325	328	PF01048	0.457
MOD_GlcNHglycan	348	351	PF01048	0.441
MOD_GlcNHglycan	376	379	PF01048	0.447
MOD_GlcNHglycan	391	394	PF01048	0.302
MOD_GlcNHglycan	41	44	PF01048	0.512
MOD_GlcNHglycan	461	464	PF01048	0.454
MOD_GlcNHglycan	561	564	PF01048	0.470
MOD_GlcNHglycan	570	573	PF01048	0.470
MOD_GlcNHglycan	607	610	PF01048	0.505
MOD_GSK3_1	261	268	PF00069	0.536
MOD_GSK3_1	39	46	PF00069	0.414
MOD_GSK3_1	617	624	PF00069	0.451
MOD_N-GLC_1	334	339	PF02516	0.441
MOD_N-GLC_1	556	561	PF02516	0.361
MOD_NEK2_1	251	256	PF00069	0.596
MOD_NEK2_1	288	293	PF00069	0.486
MOD_NEK2_1	459	464	PF00069	0.441
MOD_NEK2_1	607	612	PF00069	0.457
MOD_PIKK_1	278	284	PF00454	0.441
MOD_PK_1	302	308	PF00069	0.202
MOD_PKA_2	314	320	PF00069	0.463
MOD_PKA_2	374	380	PF00069	0.470
MOD_PKA_2	5	11	PF00069	0.466
MOD_Plk_1	154	160	PF00069	0.446
MOD_Plk_1	43	49	PF00069	0.430
MOD_Plk_1	556	562	PF00069	0.361
MOD_Plk_1	614	620	PF00069	0.441
MOD_Plk_4	261	267	PF00069	0.518
MOD_Plk_4	320	326	PF00069	0.441
MOD_Plk_4	349	355	PF00069	0.441
MOD_Plk_4	454	460	PF00069	0.441
MOD_Plk_4	614	620	PF00069	0.441
MOD_ProDKin_1	478	484	PF00069	0.441
MOD_ProDKin_1	621	627	PF00069	0.447
MOD_SUMO_for_1	110	113	PF00179	0.193
MOD_SUMO_for_1	530	533	PF00179	0.441
MOD_SUMO_rev_2	254	263	PF00179	0.548
MOD_SUMO_rev_2	296	301	PF00179	0.441
MOD_SUMO_rev_2	336	343	PF00179	0.441
MOD_SUMO_rev_2	420	428	PF00179	0.377
MOD_SUMO_rev_2	608	618	PF00179	0.441
TRG_DiLeu_BaEn_1	113	118	PF01217	0.364
TRG_ENDOCYTIC_2	141	144	PF00928	0.364
TRG_ENDOCYTIC_2	410	413	PF00928	0.441
TRG_ENDOCYTIC_2	57	60	PF00928	0.364
TRG_ENDOCYTIC_2	582	585	PF00928	0.441
TRG_ENDOCYTIC_2	594	597	PF00928	0.279
TRG_ER_diArg_1	221	223	PF00400	0.521
TRG_ER_diArg_1	255	258	PF00400	0.670
TRG_ER_diArg_1	312	315	PF00400	0.441
TRG_ER_diArg_1	449	452	PF00400	0.441
TRG_ER_diArg_1	64	66	PF00400	0.396

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P5Y6	Leptomonas seymouri	77%	100%
A0A0N1PC88	Leptomonas seymouri	29%	91%
A0A0S4J0Y2	Bodo saltans	29%	74%
A0A0S4JSQ6	Bodo saltans	53%	100%
A0A1X0ND08	Trypanosomatidae	28%	91%
A0A1X0NR71	Trypanosomatidae	63%	100%
A0A3Q8IGF4	Leishmania donovani	95%	100%
A0A3Q8IGS6	Leishmania donovani	28%	91%
A0A3R7LYC7	Trypanosoma rangeli	59%	100%
A4HFW1	Leishmania braziliensis	86%	100%
A4I1T8	Leishmania infantum	28%	91%
A4I2Z3	Leishmania infantum	95%	100%
C9ZJE1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	61%	100%
E9AXX3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	98%
O60993	Crithidia fasciculata	78%	100%
P0AES0	Escherichia coli (strain K12)	33%	100%
P0AES1	Shigella flexneri	33%	100%
P90518	Crithidia fasciculata	29%	91%
Q711P7	Leishmania major	94%	100%
Q9GT49	Trypanosoma cruzi (strain CL Brener)	60%	100%
V5ASH7	Trypanosoma cruzi	26%	91%
V5AYP7	Trypanosoma cruzi	60%	100%