CRISPR-associated protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Repeat of unknown function (DUF1126)/EF-hand domain pair, putative

Species:

Leishmania mexicana

UniProt:

E9AYY9_LEIMU

TriTrypDb:

LmxM.27.0870

Length:

756

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005819	spindle	5	1
GO:0005930	axoneme	2	1
GO:0043226	organelle	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0072686	mitotic spindle	6	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AYY9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AYY9

Function

Biological processes
Term	Name	Level	Count
GO:0000226	microtubule cytoskeleton organization	3	1
GO:0000281	mitotic cytokinesis	4	1
GO:0000910	cytokinesis	3	1
GO:0001539	cilium or flagellum-dependent cell motility	3	1
GO:0006996	organelle organization	4	1
GO:0007010	cytoskeleton organization	5	1
GO:0007017	microtubule-based process	2	1
GO:0007051	spindle organization	3	1
GO:0007052	mitotic spindle organization	4	1
GO:0009987	cellular process	1	1
GO:0016043	cellular component organization	3	1
GO:0022402	cell cycle process	2	1
GO:0048870	cell motility	2	1
GO:0060285	cilium-dependent cell motility	4	1
GO:0061640	cytoskeleton-dependent cytokinesis	4	1
GO:0071840	cellular component organization or biogenesis	2	1
GO:1902850	microtubule cytoskeleton organization involved in mitosis	4	1
GO:1903047	mitotic cell cycle process	3	1

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	6
GO:0005509	calcium ion binding	5	6
GO:0005515	protein binding	2	1
GO:0008092	cytoskeletal protein binding	3	1
GO:0015631	tubulin binding	4	1
GO:0043014	alpha-tubulin binding	5	1
GO:0043167	ion binding	2	6
GO:0043169	cation binding	3	6
GO:0046872	metal ion binding	4	6

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	56	60	PF00656	0.539
CLV_NRD_NRD_1	115	117	PF00675	0.254
CLV_NRD_NRD_1	251	253	PF00675	0.334
CLV_NRD_NRD_1	29	31	PF00675	0.546
CLV_NRD_NRD_1	34	36	PF00675	0.550
CLV_NRD_NRD_1	396	398	PF00675	0.568
CLV_NRD_NRD_1	416	418	PF00675	0.253
CLV_NRD_NRD_1	441	443	PF00675	0.254
CLV_NRD_NRD_1	478	480	PF00675	0.306
CLV_NRD_NRD_1	532	534	PF00675	0.396
CLV_NRD_NRD_1	646	648	PF00675	0.617
CLV_NRD_NRD_1	669	671	PF00675	0.413
CLV_NRD_NRD_1	699	701	PF00675	0.440
CLV_PCSK_KEX2_1	148	150	PF00082	0.254
CLV_PCSK_KEX2_1	29	31	PF00082	0.546
CLV_PCSK_KEX2_1	34	36	PF00082	0.550
CLV_PCSK_KEX2_1	396	398	PF00082	0.568
CLV_PCSK_KEX2_1	416	418	PF00082	0.253
CLV_PCSK_KEX2_1	441	443	PF00082	0.254
CLV_PCSK_KEX2_1	478	480	PF00082	0.254
CLV_PCSK_KEX2_1	669	671	PF00082	0.413
CLV_PCSK_KEX2_1	699	701	PF00082	0.517
CLV_PCSK_PC1ET2_1	148	150	PF00082	0.254
CLV_PCSK_PC7_1	30	36	PF00082	0.654
CLV_PCSK_PC7_1	474	480	PF00082	0.254
CLV_PCSK_SKI1_1	117	121	PF00082	0.237
CLV_PCSK_SKI1_1	188	192	PF00082	0.441
CLV_PCSK_SKI1_1	245	249	PF00082	0.335
CLV_PCSK_SKI1_1	252	256	PF00082	0.294
CLV_PCSK_SKI1_1	271	275	PF00082	0.285
CLV_PCSK_SKI1_1	308	312	PF00082	0.237
CLV_PCSK_SKI1_1	354	358	PF00082	0.254
CLV_PCSK_SKI1_1	442	446	PF00082	0.254
CLV_PCSK_SKI1_1	495	499	PF00082	0.302
CLV_PCSK_SKI1_1	536	540	PF00082	0.255
CLV_PCSK_SKI1_1	55	59	PF00082	0.654
CLV_PCSK_SKI1_1	670	674	PF00082	0.425
CLV_PCSK_SKI1_1	717	721	PF00082	0.499
CLV_Separin_Metazoa	26	30	PF03568	0.548
DEG_SIAH_1	198	206	PF03145	0.600
DOC_CDC14_PxL_1	15	23	PF14671	0.659
DOC_CYCLIN_RxL_1	185	193	PF00134	0.639
DOC_CYCLIN_RxL_1	568	579	PF00134	0.467
DOC_CYCLIN_RxL_1	667	676	PF00134	0.411
DOC_CYCLIN_RxL_1	695	708	PF00134	0.521
DOC_MAPK_FxFP_2	141	144	PF00069	0.451
DOC_MAPK_gen_1	114	122	PF00069	0.457
DOC_MAPK_gen_1	242	250	PF00069	0.452
DOC_MAPK_gen_1	34	42	PF00069	0.662
DOC_MAPK_gen_1	439	449	PF00069	0.461
DOC_MAPK_MEF2A_6	114	122	PF00069	0.506
DOC_MAPK_MEF2A_6	441	449	PF00069	0.502
DOC_PP4_FxxP_1	13	16	PF00568	0.528
DOC_PP4_FxxP_1	141	144	PF00568	0.451
DOC_PP4_FxxP_1	389	392	PF00568	0.634
DOC_USP7_MATH_1	450	454	PF00917	0.492
DOC_USP7_MATH_1	553	557	PF00917	0.381
DOC_USP7_MATH_1	612	616	PF00917	0.528
DOC_USP7_MATH_1	631	635	PF00917	0.634
DOC_USP7_MATH_1	653	657	PF00917	0.509
DOC_USP7_MATH_1	684	688	PF00917	0.540
DOC_USP7_MATH_1	725	729	PF00917	0.530
DOC_USP7_MATH_1	748	752	PF00917	0.536
DOC_USP7_UBL2_3	435	439	PF12436	0.557
DOC_USP7_UBL2_3	530	534	PF12436	0.539
DOC_WW_Pin1_4	155	160	PF00397	0.443
DOC_WW_Pin1_4	227	232	PF00397	0.559
DOC_WW_Pin1_4	234	239	PF00397	0.514
DOC_WW_Pin1_4	376	381	PF00397	0.511
DOC_WW_Pin1_4	382	387	PF00397	0.591
LIG_14-3-3_CanoR_1	419	425	PF00244	0.414
LIG_14-3-3_CanoR_1	55	64	PF00244	0.565
LIG_14-3-3_CanoR_1	726	730	PF00244	0.440
LIG_AP2alpha_2	184	186	PF02296	0.557
LIG_AP2alpha_2	298	300	PF02296	0.454
LIG_BIR_II_1	1	5	PF00653	0.555
LIG_BIR_III_2	182	186	PF00653	0.567
LIG_BRCT_BRCA1_1	283	287	PF00533	0.502
LIG_BRCT_BRCA1_1	315	319	PF00533	0.534
LIG_BRCT_BRCA1_1	325	329	PF00533	0.468
LIG_BRCT_BRCA1_1	499	503	PF00533	0.534
LIG_BRCT_BRCA1_1	690	694	PF00533	0.479
LIG_Clathr_ClatBox_1	428	432	PF01394	0.357
LIG_eIF4E_1	683	689	PF01652	0.535
LIG_FHA_1	110	116	PF00498	0.494
LIG_FHA_1	126	132	PF00498	0.407
LIG_FHA_1	175	181	PF00498	0.450
LIG_FHA_1	185	191	PF00498	0.526
LIG_FHA_1	262	268	PF00498	0.468
LIG_FHA_1	541	547	PF00498	0.476
LIG_FHA_1	89	95	PF00498	0.356
LIG_FHA_2	258	264	PF00498	0.534
LIG_FHA_2	326	332	PF00498	0.461
LIG_FHA_2	337	343	PF00498	0.413
LIG_FHA_2	393	399	PF00498	0.684
LIG_FHA_2	401	407	PF00498	0.490
LIG_FHA_2	54	60	PF00498	0.517
LIG_FHA_2	576	582	PF00498	0.470
LIG_LIR_Apic_2	12	16	PF02991	0.534
LIG_LIR_Apic_2	139	144	PF02991	0.451
LIG_LIR_Gen_1	206	217	PF02991	0.500
LIG_LIR_Gen_1	244	255	PF02991	0.458
LIG_LIR_Gen_1	270	280	PF02991	0.506
LIG_LIR_Gen_1	500	510	PF02991	0.508
LIG_LIR_Gen_1	564	573	PF02991	0.400
LIG_LIR_Gen_1	598	607	PF02991	0.466
LIG_LIR_Gen_1	619	624	PF02991	0.402
LIG_LIR_Gen_1	661	666	PF02991	0.420
LIG_LIR_Gen_1	676	684	PF02991	0.355
LIG_LIR_Gen_1	728	736	PF02991	0.497
LIG_LIR_Nem_3	177	181	PF02991	0.454
LIG_LIR_Nem_3	244	250	PF02991	0.355
LIG_LIR_Nem_3	270	276	PF02991	0.464
LIG_LIR_Nem_3	298	303	PF02991	0.454
LIG_LIR_Nem_3	360	364	PF02991	0.463
LIG_LIR_Nem_3	376	381	PF02991	0.370
LIG_LIR_Nem_3	455	460	PF02991	0.481
LIG_LIR_Nem_3	500	506	PF02991	0.508
LIG_LIR_Nem_3	564	569	PF02991	0.398
LIG_LIR_Nem_3	593	599	PF02991	0.460
LIG_LIR_Nem_3	619	623	PF02991	0.407
LIG_LIR_Nem_3	661	665	PF02991	0.414
LIG_LIR_Nem_3	676	680	PF02991	0.282
LIG_LIR_Nem_3	728	732	PF02991	0.504
LIG_PCNA_APIM_2	424	430	PF02747	0.349
LIG_Pex14_1	620	624	PF04695	0.530
LIG_Pex14_2	673	677	PF04695	0.418
LIG_SH2_CRK	378	382	PF00017	0.493
LIG_SH2_NCK_1	378	382	PF00017	0.400
LIG_SH2_NCK_1	599	603	PF00017	0.583
LIG_SH2_SRC	599	602	PF00017	0.588
LIG_SH2_SRC	624	627	PF00017	0.579
LIG_SH2_STAP1	599	603	PF00017	0.471
LIG_SH2_STAP1	729	733	PF00017	0.491
LIG_SH2_STAP1	742	746	PF00017	0.531
LIG_SH2_STAT3	352	355	PF00017	0.557
LIG_SH2_STAT5	100	103	PF00017	0.468
LIG_SH2_STAT5	123	126	PF00017	0.534
LIG_SH2_STAT5	209	212	PF00017	0.526
LIG_SH2_STAT5	278	281	PF00017	0.461
LIG_SH2_STAT5	338	341	PF00017	0.454
LIG_SH2_STAT5	364	367	PF00017	0.552
LIG_SH2_STAT5	378	381	PF00017	0.464
LIG_SH2_STAT5	448	451	PF00017	0.502
LIG_SH2_STAT5	484	487	PF00017	0.460
LIG_SH2_STAT5	491	494	PF00017	0.446
LIG_SH2_STAT5	588	591	PF00017	0.420
LIG_SH2_STAT5	596	599	PF00017	0.451
LIG_SH3_3	128	134	PF00018	0.534
LIG_SH3_3	13	19	PF00018	0.525
LIG_SH3_3	149	155	PF00018	0.506
LIG_SH3_3	164	170	PF00018	0.488
LIG_SH3_3	385	391	PF00018	0.495
LIG_SUMO_SIM_anti_2	581	587	PF11976	0.395
LIG_SUMO_SIM_par_1	745	751	PF11976	0.377
LIG_TRAF2_1	108	111	PF00917	0.405
LIG_TRAF2_1	194	197	PF00917	0.462
LIG_TRAF2_1	578	581	PF00917	0.367
LIG_TRAF2_1	598	601	PF00917	0.292
LIG_TRAF2_1	708	711	PF00917	0.522
LIG_TRAF2_1	728	731	PF00917	0.401
LIG_TYR_ITIM	597	602	PF00017	0.562
LIG_TYR_ITSM	374	381	PF00017	0.439
LIG_UBA3_1	582	591	PF00899	0.408
LIG_WRC_WIRS_1	632	637	PF05994	0.372
MOD_CK1_1	281	287	PF00069	0.506
MOD_CK1_1	323	329	PF00069	0.524
MOD_CK1_1	400	406	PF00069	0.573
MOD_CK1_1	541	547	PF00069	0.414
MOD_CK1_1	595	601	PF00069	0.469
MOD_CK1_1	705	711	PF00069	0.492
MOD_CK2_1	105	111	PF00069	0.457
MOD_CK2_1	127	133	PF00069	0.454
MOD_CK2_1	19	25	PF00069	0.517
MOD_CK2_1	325	331	PF00069	0.482
MOD_CK2_1	392	398	PF00069	0.634
MOD_CK2_1	400	406	PF00069	0.476
MOD_CK2_1	452	458	PF00069	0.485
MOD_CK2_1	575	581	PF00069	0.372
MOD_CK2_1	595	601	PF00069	0.283
MOD_CK2_1	705	711	PF00069	0.445
MOD_CK2_1	725	731	PF00069	0.399
MOD_GlcNHglycan	107	110	PF01048	0.291
MOD_GlcNHglycan	137	141	PF01048	0.243
MOD_GlcNHglycan	294	297	PF01048	0.254
MOD_GlcNHglycan	313	316	PF01048	0.323
MOD_GlcNHglycan	463	466	PF01048	0.257
MOD_GSK3_1	105	112	PF00069	0.512
MOD_GSK3_1	125	132	PF00069	0.388
MOD_GSK3_1	203	210	PF00069	0.525
MOD_GSK3_1	222	229	PF00069	0.396
MOD_GSK3_1	257	264	PF00069	0.534
MOD_GSK3_1	319	326	PF00069	0.500
MOD_GSK3_1	332	339	PF00069	0.468
MOD_GSK3_1	505	512	PF00069	0.449
MOD_GSK3_1	612	619	PF00069	0.514
MOD_GSK3_1	684	691	PF00069	0.502
MOD_GSK3_1	738	745	PF00069	0.499
MOD_GSK3_1	748	755	PF00069	0.590
MOD_GSK3_1	84	91	PF00069	0.449
MOD_N-GLC_1	616	621	PF02516	0.499
MOD_N-GLC_1	736	741	PF02516	0.408
MOD_NEK2_1	1	6	PF00069	0.564
MOD_NEK2_1	267	272	PF00069	0.506
MOD_NEK2_1	319	324	PF00069	0.534
MOD_NEK2_1	373	378	PF00069	0.463
MOD_NEK2_1	420	425	PF00069	0.516
MOD_NEK2_1	538	543	PF00069	0.406
MOD_NEK2_1	702	707	PF00069	0.462
MOD_NEK2_1	736	741	PF00069	0.426
MOD_NEK2_2	208	213	PF00069	0.545
MOD_NEK2_2	325	330	PF00069	0.472
MOD_NEK2_2	65	70	PF00069	0.729
MOD_PIKK_1	1	7	PF00454	0.596
MOD_PKA_2	241	247	PF00069	0.446
MOD_PKA_2	461	467	PF00069	0.468
MOD_PKA_2	646	652	PF00069	0.626
MOD_PKA_2	725	731	PF00069	0.441
MOD_Plk_1	257	263	PF00069	0.534
MOD_Plk_1	319	325	PF00069	0.516
MOD_Plk_1	400	406	PF00069	0.573
MOD_Plk_1	450	456	PF00069	0.454
MOD_Plk_1	509	515	PF00069	0.534
MOD_Plk_1	53	59	PF00069	0.650
MOD_Plk_1	616	622	PF00069	0.496
MOD_Plk_1	702	708	PF00069	0.514
MOD_Plk_2-3	703	709	PF00069	0.493
MOD_Plk_2-3	84	90	PF00069	0.415
MOD_Plk_4	169	175	PF00069	0.534
MOD_Plk_4	231	237	PF00069	0.638
MOD_Plk_4	263	269	PF00069	0.515
MOD_Plk_4	272	278	PF00069	0.449
MOD_Plk_4	332	338	PF00069	0.464
MOD_Plk_4	420	426	PF00069	0.534
MOD_Plk_4	452	458	PF00069	0.504
MOD_Plk_4	565	571	PF00069	0.462
MOD_Plk_4	581	587	PF00069	0.261
MOD_Plk_4	592	598	PF00069	0.446
MOD_Plk_4	631	637	PF00069	0.619
MOD_Plk_4	684	690	PF00069	0.426
MOD_Plk_4	742	748	PF00069	0.370
MOD_ProDKin_1	155	161	PF00069	0.443
MOD_ProDKin_1	227	233	PF00069	0.559
MOD_ProDKin_1	234	240	PF00069	0.508
MOD_ProDKin_1	376	382	PF00069	0.514
MOD_SUMO_rev_2	249	255	PF00179	0.534
MOD_SUMO_rev_2	432	440	PF00179	0.557
MOD_SUMO_rev_2	634	642	PF00179	0.558
MOD_SUMO_rev_2	643	649	PF00179	0.501
MOD_SUMO_rev_2	687	696	PF00179	0.432
TRG_DiLeu_BaEn_1	731	736	PF01217	0.486
TRG_DiLeu_BaEn_4	710	716	PF01217	0.500
TRG_DiLeu_LyEn_5	731	736	PF01217	0.519
TRG_ENDOCYTIC_2	209	212	PF00928	0.496
TRG_ENDOCYTIC_2	277	280	PF00928	0.459
TRG_ENDOCYTIC_2	378	381	PF00928	0.439
TRG_ENDOCYTIC_2	599	602	PF00928	0.477
TRG_ENDOCYTIC_2	729	732	PF00928	0.461
TRG_ER_diArg_1	212	215	PF00400	0.532
TRG_ER_diArg_1	28	30	PF00400	0.548
TRG_ER_diArg_1	306	309	PF00400	0.459
TRG_ER_diArg_1	415	417	PF00400	0.567
TRG_ER_diArg_1	440	442	PF00400	0.454
TRG_ER_diArg_1	477	479	PF00400	0.507
TRG_ER_diArg_1	698	700	PF00400	0.410
TRG_NLS_MonoExtN_4	530	537	PF00514	0.349
TRG_Pf-PMV_PEXEL_1	188	193	PF00026	0.541
TRG_Pf-PMV_PEXEL_1	397	401	PF00026	0.637
TRG_Pf-PMV_PEXEL_1	55	59	PF00026	0.440

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P5H4	Leptomonas seymouri	23%	90%
A0A0N1HZ98	Leptomonas seymouri	27%	98%
A0A0N1I3M2	Leptomonas seymouri	75%	99%
A0A0N1PBW1	Leptomonas seymouri	27%	100%
A0A0S4IJW8	Bodo saltans	26%	92%
A0A0S4IQM0	Bodo saltans	24%	100%
A0A0S4J8J7	Bodo saltans	27%	82%
A0A1X0NEV8	Trypanosomatidae	24%	95%
A0A1X0NLI2	Trypanosomatidae	26%	100%
A0A1X0NMQ3	Trypanosomatidae	24%	97%
A0A1X0P419	Trypanosomatidae	63%	99%
A0A3Q1N1R0	Bos taurus	31%	100%
A0A3Q8IDH4	Leishmania donovani	25%	98%
A0A3Q8IPU3	Leishmania donovani	95%	100%
A0A3R7KM50	Trypanosoma rangeli	27%	95%
A0A3R7L048	Trypanosoma rangeli	59%	100%
A0A3S7XC76	Leishmania donovani	26%	100%
A0A422N0Z6	Trypanosoma rangeli	24%	97%
A0A422NK00	Trypanosoma rangeli	29%	100%
A4HE78	Leishmania braziliensis	26%	100%
A4HFK9	Leishmania braziliensis	88%	100%
A4HQF8	Leishmania braziliensis	27%	100%
A4I1J2	Leishmania infantum	25%	98%
A4IC94	Leishmania infantum	26%	100%
C9ZJX4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	25%	94%
C9ZPE7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	25%	97%
D0A3K7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	25%	100%
D0A5T5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	55%	100%
E1BKH1	Bos taurus	30%	100%
E9AD64	Leishmania major	95%	100%
E9AHF6	Leishmania infantum	95%	100%
E9AI34	Leishmania braziliensis	23%	100%
E9AMI2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	22%	100%
E9AU74	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	96%
E9AXM7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	24%	100%
Q32TF8	Danio rerio	30%	100%
Q32TG3	Gallus gallus	28%	100%
Q4Q0C0	Leishmania major	27%	97%
Q4Q9U5	Leishmania major	25%	100%
Q4QI57	Leishmania major	22%	87%
Q5JST6	Homo sapiens	28%	100%
Q5JVL4	Homo sapiens	30%	100%
Q9D485	Mus musculus	29%	100%
Q9D9T8	Mus musculus	30%	100%
V5ASV2	Trypanosoma cruzi	26%	100%
V5D3X9	Trypanosoma cruzi	24%	97%
V5DA42	Trypanosoma cruzi	59%	100%