Putative metallopeptidase (Metallo-peptidase, clan mp, family m67)

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Lys-63-specific deubiquitinase BRCC36, putative

Species:

Leishmania mexicana

UniProt:

E9AYM4_LEIMU

TriTrypDb:

LmxM.26.2430

Length:

335

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 7
NetGPI	no	yes: 0, no: 7

Cellular components
Term	Name	Level	Count
GO:0032991	protein-containing complex	1	2
GO:0070531	BRCA1-A complex	3	2
GO:0070552	BRISC complex	3	2
GO:0140513	nuclear protein-containing complex	2	2

Expansion

Sequence features

E9AYM4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AYM4

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006259	DNA metabolic process	4	2
GO:0006281	DNA repair	5	2
GO:0006302	double-strand break repair	6	1
GO:0006508	proteolysis	4	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0006950	response to stress	2	2
GO:0006974	DNA damage response	4	2
GO:0008152	metabolic process	1	2
GO:0009987	cellular process	1	2
GO:0016570	histone modification	5	1
GO:0016578	histone deubiquitination	6	1
GO:0016579	protein deubiquitination	6	2
GO:0019538	protein metabolic process	3	2
GO:0033554	cellular response to stress	3	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0036211	protein modification process	4	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0046483	heterocycle metabolic process	3	2
GO:0050896	response to stimulus	1	2
GO:0051716	cellular response to stimulus	2	2
GO:0070536	protein K63-linked deubiquitination	7	2
GO:0070537	histone H2A K63-linked deubiquitination	7	1
GO:0070646	protein modification by small protein removal	5	2
GO:0070647	protein modification by small protein conjugation or removal	5	2
GO:0071704	organic substance metabolic process	2	2
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2
GO:1901564	organonitrogen compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	8
GO:0008233	peptidase activity	3	8
GO:0008237	metallopeptidase activity	4	8
GO:0016787	hydrolase activity	2	8
GO:0140096	catalytic activity, acting on a protein	2	8
GO:0005488	binding	1	1
GO:0005515	protein binding	2	1
GO:0031593	polyubiquitin modification-dependent protein binding	4	1
GO:0140030	modification-dependent protein binding	3	1
GO:0004843	cysteine-type deubiquitinase activity	5	1
GO:0008234	cysteine-type peptidase activity	4	1
GO:0019783	ubiquitin-like protein peptidase activity	4	1
GO:0101005	deubiquitinase activity	5	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	259	263	PF00656	0.528
CLV_C14_Caspase3-7	271	275	PF00656	0.459
CLV_C14_Caspase3-7	74	78	PF00656	0.480
CLV_NRD_NRD_1	300	302	PF00675	0.561
CLV_PCSK_KEX2_1	107	109	PF00082	0.497
CLV_PCSK_PC1ET2_1	107	109	PF00082	0.529
CLV_PCSK_SKI1_1	35	39	PF00082	0.465
DEG_APCC_DBOX_1	16	24	PF00400	0.513
DEG_Nend_UBRbox_3	1	3	PF02207	0.715
DOC_MAPK_gen_1	279	289	PF00069	0.557
DOC_USP7_MATH_1	100	104	PF00917	0.584
DOC_USP7_MATH_1	13	17	PF00917	0.411
DOC_USP7_MATH_1	169	173	PF00917	0.199
DOC_USP7_MATH_1	275	279	PF00917	0.577
DOC_USP7_MATH_1	305	309	PF00917	0.296
DOC_USP7_MATH_1	4	8	PF00917	0.566
DOC_WW_Pin1_4	246	251	PF00397	0.474
LIG_14-3-3_CanoR_1	146	154	PF00244	0.403
LIG_14-3-3_CanoR_1	17	25	PF00244	0.504
LIG_14-3-3_CanoR_1	35	45	PF00244	0.519
LIG_Actin_WH2_2	22	37	PF00022	0.430
LIG_BRCT_BRCA1_1	232	236	PF00533	0.568
LIG_Clathr_ClatBox_1	289	293	PF01394	0.553
LIG_EH1_1	289	297	PF00400	0.451
LIG_FHA_1	149	155	PF00498	0.331
LIG_FHA_1	247	253	PF00498	0.428
LIG_FHA_1	88	94	PF00498	0.567
LIG_FHA_2	265	271	PF00498	0.517
LIG_FHA_2	298	304	PF00498	0.541
LIG_FHA_2	38	44	PF00498	0.386
LIG_LIR_Gen_1	233	244	PF02991	0.584
LIG_LIR_Nem_3	187	193	PF02991	0.348
LIG_LIR_Nem_3	233	239	PF02991	0.544
LIG_SH2_STAT5	113	116	PF00017	0.460
LIG_SH2_STAT5	156	159	PF00017	0.427
LIG_SH3_3	162	168	PF00018	0.377
LIG_SH3_3	220	226	PF00018	0.561
LIG_SH3_3	293	299	PF00018	0.491
LIG_UBA3_1	252	258	PF00899	0.274
MOD_CDK_SPK_2	246	251	PF00069	0.471
MOD_CK1_1	133	139	PF00069	0.392
MOD_CK1_1	7	13	PF00069	0.449
MOD_CK2_1	136	142	PF00069	0.349
MOD_CK2_1	264	270	PF00069	0.514
MOD_CK2_1	297	303	PF00069	0.502
MOD_CK2_1	37	43	PF00069	0.377
MOD_GlcNHglycan	103	106	PF01048	0.644
MOD_GlcNHglycan	131	135	PF01048	0.557
MOD_GlcNHglycan	138	141	PF01048	0.330
MOD_GlcNHglycan	187	190	PF01048	0.386
MOD_GlcNHglycan	270	273	PF01048	0.556
MOD_GlcNHglycan	85	88	PF01048	0.738
MOD_GSK3_1	148	155	PF00069	0.385
MOD_GSK3_1	193	200	PF00069	0.375
MOD_GSK3_1	264	271	PF00069	0.532
MOD_GSK3_1	83	90	PF00069	0.622
MOD_N-GLC_1	7	12	PF02516	0.624
MOD_N-GLC_1	87	92	PF02516	0.460
MOD_NEK2_1	193	198	PF00069	0.336
MOD_NEK2_1	245	250	PF00069	0.396
MOD_NEK2_1	323	328	PF00069	0.592
MOD_NEK2_1	6	11	PF00069	0.456
MOD_NEK2_2	207	212	PF00069	0.262
MOD_PIKK_1	175	181	PF00454	0.314
MOD_PIKK_1	71	77	PF00454	0.483
MOD_PKA_2	101	107	PF00069	0.446
MOD_PKA_2	145	151	PF00069	0.330
MOD_PKA_2	23	29	PF00069	0.426
MOD_PKA_2	268	274	PF00069	0.598
MOD_Plk_1	200	206	PF00069	0.390
MOD_Plk_1	7	13	PF00069	0.568
MOD_Plk_4	152	158	PF00069	0.423
MOD_Plk_4	193	199	PF00069	0.435
MOD_ProDKin_1	246	252	PF00069	0.467
MOD_SUMO_for_1	106	109	PF00179	0.560
TRG_DiLeu_BaEn_1	44	49	PF01217	0.414
TRG_DiLeu_BaLyEn_6	248	253	PF01217	0.404
TRG_DiLeu_BaLyEn_6	307	312	PF01217	0.575
TRG_Pf-PMV_PEXEL_1	282	286	PF00026	0.421

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PFL1	Leptomonas seymouri	53%	100%
A0A1X0P845	Trypanosomatidae	37%	100%
A4HF88	Leishmania braziliensis	76%	100%
C9ZX24	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	39%	100%
E2AXC7	Camponotus floridanus	31%	100%
Q4Q8U8	Leishmania major	89%	100%
V5BGS3	Trypanosoma cruzi	41%	100%