Protein disulfide-isomerase

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

protein disulfide isomerase, putative

Species:

Leishmania mexicana

UniProt:

E9AY45_LEIMU

TriTrypDb:

LmxM.26.0660

Length:

379

Annotations

LeishMANIAdb annotations

Appears to be a family of secreted proteins, probably ER-localized, like its homologs in other Eukaryotes.. Seems to carry a putative KDEL-like signal on its C-terminus.. Localization: Secreted (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 4
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 16

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	yes	yes: 9, no: 2
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005783	endoplasmic reticulum	5	12
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0043229	intracellular organelle	3	12
GO:0043231	intracellular membrane-bounded organelle	4	12
GO:0110165	cellular anatomical entity	1	12
GO:0005794	Golgi apparatus	5	1

Expansion

Sequence features

E9AY45

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AY45

Function

Biological processes
Term	Name	Level	Count
GO:0006457	protein folding	2	1
GO:0009987	cellular process	1	1

Molecular functions
Term	Name	Level	Count
GO:0003756	protein disulfide isomerase activity	3	10
GO:0003824	catalytic activity	1	11
GO:0016853	isomerase activity	2	11
GO:0016860	intramolecular oxidoreductase activity	3	10
GO:0016864	intramolecular oxidoreductase activity, transposing S-S bonds	4	10
GO:0140096	catalytic activity, acting on a protein	2	10

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_MEL_PAP_1	103	109	PF00089	0.368
CLV_NRD_NRD_1	222	224	PF00675	0.461
CLV_NRD_NRD_1	360	362	PF00675	0.474
CLV_PCSK_FUR_1	347	351	PF00082	0.498
CLV_PCSK_KEX2_1	105	107	PF00082	0.461
CLV_PCSK_KEX2_1	349	351	PF00082	0.498
CLV_PCSK_KEX2_1	360	362	PF00082	0.413
CLV_PCSK_PC1ET2_1	105	107	PF00082	0.370
CLV_PCSK_PC1ET2_1	349	351	PF00082	0.521
CLV_PCSK_SKI1_1	176	180	PF00082	0.312
CLV_PCSK_SKI1_1	191	195	PF00082	0.301
CLV_PCSK_SKI1_1	199	203	PF00082	0.357
CLV_PCSK_SKI1_1	224	228	PF00082	0.370
CLV_PCSK_SKI1_1	52	56	PF00082	0.405
CLV_PCSK_SKI1_1	87	91	PF00082	0.379
DOC_CYCLIN_yClb5_NLxxxL_5	255	261	PF00134	0.370
DOC_MAPK_gen_1	360	368	PF00069	0.229
DOC_MAPK_gen_1	50	58	PF00069	0.448
DOC_MAPK_MEF2A_6	3	11	PF00069	0.591
DOC_MAPK_MEF2A_6	361	370	PF00069	0.309
DOC_PP2B_LxvP_1	150	153	PF13499	0.401
DOC_PP4_FxxP_1	182	185	PF00568	0.301
DOC_PP4_FxxP_1	58	61	PF00568	0.301
DOC_SPAK_OSR1_1	106	110	PF12202	0.293
DOC_USP7_MATH_1	174	178	PF00917	0.245
DOC_USP7_MATH_1	296	300	PF00917	0.299
DOC_USP7_UBL2_3	172	176	PF12436	0.410
DOC_WW_Pin1_4	30	35	PF00397	0.464
LIG_14-3-3_CanoR_1	223	231	PF00244	0.370
LIG_14-3-3_CanoR_1	3	8	PF00244	0.574
LIG_14-3-3_CanoR_1	347	353	PF00244	0.354
LIG_Actin_WH2_2	134	151	PF00022	0.439
LIG_FHA_1	31	37	PF00498	0.264
LIG_FHA_1	8	14	PF00498	0.404
LIG_FHA_2	131	137	PF00498	0.410
LIG_FHA_2	163	169	PF00498	0.439
LIG_FHA_2	316	322	PF00498	0.316
LIG_GBD_Chelix_1	7	15	PF00786	0.378
LIG_Integrin_isoDGR_2	253	255	PF01839	0.370
LIG_LIR_Apic_2	114	120	PF02991	0.358
LIG_LIR_Apic_2	236	241	PF02991	0.448
LIG_LIR_Apic_2	57	61	PF02991	0.293
LIG_LIR_Gen_1	165	174	PF02991	0.311
LIG_LIR_Gen_1	196	204	PF02991	0.243
LIG_LIR_Gen_1	258	268	PF02991	0.412
LIG_LIR_Gen_1	42	48	PF02991	0.388
LIG_LIR_Gen_1	72	80	PF02991	0.336
LIG_LIR_Nem_3	110	115	PF02991	0.378
LIG_LIR_Nem_3	135	140	PF02991	0.324
LIG_LIR_Nem_3	165	170	PF02991	0.311
LIG_LIR_Nem_3	196	200	PF02991	0.243
LIG_LIR_Nem_3	232	237	PF02991	0.237
LIG_LIR_Nem_3	258	263	PF02991	0.370
LIG_LIR_Nem_3	279	285	PF02991	0.485
LIG_LIR_Nem_3	42	47	PF02991	0.388
LIG_LIR_Nem_3	72	76	PF02991	0.317
LIG_Pex14_2	182	186	PF04695	0.303
LIG_Pex14_2	58	62	PF04695	0.278
LIG_RPA_C_Fungi	209	221	PF08784	0.370
LIG_SH2_CRK	197	201	PF00017	0.381
LIG_SH2_CRK	282	286	PF00017	0.273
LIG_SH2_CRK	73	77	PF00017	0.337
LIG_SH2_STAT5	117	120	PF00017	0.343
LIG_SH2_STAT5	140	143	PF00017	0.477
LIG_SH2_STAT5	238	241	PF00017	0.424
LIG_SH2_STAT5	326	329	PF00017	0.358
LIG_SH2_STAT5	340	343	PF00017	0.358
LIG_SH3_3	150	156	PF00018	0.578
LIG_SH3_3	332	338	PF00018	0.157
LIG_SUMO_SIM_anti_2	10	15	PF11976	0.591
LIG_SUMO_SIM_anti_2	177	182	PF11976	0.157
LIG_SUMO_SIM_anti_2	88	93	PF11976	0.421
LIG_TRAF2_1	97	100	PF00917	0.157
LIG_UBA3_1	370	379	PF00899	0.420
LIG_UBA3_1	89	94	PF00899	0.406
LIG_WRC_WIRS_1	260	265	PF05994	0.410
MOD_CK1_1	299	305	PF00069	0.428
MOD_CK2_1	295	301	PF00069	0.330
MOD_CK2_1	315	321	PF00069	0.348
MOD_Cter_Amidation	103	106	PF01082	0.370
MOD_Cter_Amidation	358	361	PF01082	0.157
MOD_GlcNHglycan	141	144	PF01048	0.442
MOD_GlcNHglycan	231	234	PF01048	0.362
MOD_GlcNHglycan	298	301	PF01048	0.379
MOD_GlcNHglycan	344	347	PF01048	0.385
MOD_GSK3_1	107	114	PF00069	0.368
MOD_GSK3_1	229	236	PF00069	0.308
MOD_GSK3_1	295	302	PF00069	0.349
MOD_GSK3_1	3	10	PF00069	0.414
MOD_GSK3_1	348	355	PF00069	0.413
MOD_N-GLC_1	342	347	PF02516	0.157
MOD_NEK2_1	107	112	PF00069	0.368
MOD_NEK2_1	148	153	PF00069	0.514
MOD_NEK2_1	162	167	PF00069	0.269
MOD_NEK2_1	20	25	PF00069	0.658
MOD_NEK2_1	348	353	PF00069	0.396
MOD_PIKK_1	95	101	PF00454	0.259
MOD_PK_1	3	9	PF00069	0.671
MOD_PKA_2	148	154	PF00069	0.467
MOD_Plk_1	227	233	PF00069	0.349
MOD_Plk_1	276	282	PF00069	0.401
MOD_Plk_4	111	117	PF00069	0.317
MOD_Plk_4	174	180	PF00069	0.296
MOD_Plk_4	233	239	PF00069	0.336
MOD_Plk_4	259	265	PF00069	0.341
MOD_Plk_4	3	9	PF00069	0.560
MOD_ProDKin_1	30	36	PF00069	0.457
MOD_SUMO_for_1	123	126	PF00179	0.157
TRG_ENDOCYTIC_2	197	200	PF00928	0.351
TRG_ENDOCYTIC_2	282	285	PF00928	0.376
TRG_ENDOCYTIC_2	73	76	PF00928	0.358
TRG_NES_CRM1_1	287	301	PF08389	0.249
TRG_Pf-PMV_PEXEL_1	255	259	PF00026	0.313

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HVU7	Leptomonas seymouri	51%	100%
A0A0S4IKM8	Bodo saltans	25%	98%
A0A0S4JB31	Bodo saltans	32%	85%
A0A0S4JJE1	Bodo saltans	48%	100%
A0A1X0NSF7	Trypanosomatidae	49%	99%
A0A3S7WZP2	Leishmania donovani	89%	100%
A0A422N2J8	Trypanosoma rangeli	50%	100%
A4HAT2	Leishmania braziliensis	27%	88%
A4HES1	Leishmania braziliensis	54%	100%
A4I1Z8	Leishmania infantum	89%	100%
C9ZS16	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	100%
O13704	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	27%	100%
O13811	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	38%	100%
O22263	Arabidopsis thaliana	42%	100%
O48773	Arabidopsis thaliana	32%	86%
P38660	Mesocricetus auratus	32%	86%
P38661	Medicago sativa	42%	100%
Q00216	Aspergillus niger	41%	100%
Q11067	Caenorhabditis elegans	31%	86%
Q15084	Homo sapiens	32%	86%
Q4Q9C8	Leishmania major	88%	99%
Q5I0H9	Rattus norvegicus	33%	73%
Q5R6T1	Pongo abelii	32%	86%
Q63081	Rattus norvegicus	32%	86%
Q75M08	Oryza sativa subsp. japonica	42%	100%
Q86IA3	Dictyostelium discoideum	35%	100%
Q921X9	Mus musculus	32%	73%
Q92249	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	43%	100%
Q922R8	Mus musculus	32%	86%
Q942L2	Oryza sativa subsp. japonica	40%	100%
Q9MAU6	Arabidopsis thaliana	31%	85%
Q9V438	Drosophila melanogaster	36%	88%
Q9VYV3	Drosophila melanogaster	29%	91%
V5DR98	Trypanosoma cruzi	46%	100%