Pseudouridylate synthase-like protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

pseudouridylate synthase-like protein

Species:

Leishmania mexicana

UniProt:

E9AY24_LEIMU

TriTrypDb:

LmxM.26.0420

Length:

696

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0043226	organelle	2	4
GO:0043227	membrane-bounded organelle	3	4
GO:0043229	intracellular organelle	3	4
GO:0043231	intracellular membrane-bounded organelle	4	4
GO:0110165	cellular anatomical entity	1	4

Expansion

Sequence features

E9AY24

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AY24

Function

Biological processes
Term	Name	Level	Count
GO:0001522	pseudouridine synthesis	6	6
GO:0006139	nucleobase-containing compound metabolic process	3	6
GO:0006396	RNA processing	6	6
GO:0006399	tRNA metabolic process	7	6
GO:0006400	tRNA modification	6	4
GO:0006725	cellular aromatic compound metabolic process	3	6
GO:0006807	nitrogen compound metabolic process	2	6
GO:0008033	tRNA processing	8	6
GO:0008152	metabolic process	1	6
GO:0009451	RNA modification	5	6
GO:0009987	cellular process	1	6
GO:0016070	RNA metabolic process	5	6
GO:0016071	mRNA metabolic process	6	1
GO:0016556	mRNA modification	6	1
GO:0031119	tRNA pseudouridine synthesis	7	4
GO:0034470	ncRNA processing	7	6
GO:0034641	cellular nitrogen compound metabolic process	3	6
GO:0034660	ncRNA metabolic process	6	6
GO:0043170	macromolecule metabolic process	3	6
GO:0043412	macromolecule modification	4	6
GO:0044237	cellular metabolic process	2	6
GO:0044238	primary metabolic process	2	6
GO:0046483	heterocycle metabolic process	3	6
GO:0071704	organic substance metabolic process	2	6
GO:0090304	nucleic acid metabolic process	4	6
GO:1901360	organic cyclic compound metabolic process	3	6
GO:1990481	mRNA pseudouridine synthesis	7	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	11
GO:0003723	RNA binding	4	11
GO:0003824	catalytic activity	1	11
GO:0004730	pseudouridylate synthase activity	5	8
GO:0005488	binding	1	11
GO:0009982	pseudouridine synthase activity	4	11
GO:0016829	lyase activity	2	8
GO:0016835	carbon-oxygen lyase activity	3	8
GO:0016836	hydro-lyase activity	4	8
GO:0016853	isomerase activity	2	11
GO:0016866	intramolecular transferase activity	3	11
GO:0097159	organic cyclic compound binding	2	11
GO:1901363	heterocyclic compound binding	2	11
GO:0106029	tRNA pseudouridine synthase activity	5	1
GO:0140098	catalytic activity, acting on RNA	3	1
GO:0140101	catalytic activity, acting on a tRNA	4	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	447	451	PF00656	0.310
CLV_C14_Caspase3-7	667	671	PF00656	0.611
CLV_NRD_NRD_1	13	15	PF00675	0.562
CLV_NRD_NRD_1	149	151	PF00675	0.310
CLV_NRD_NRD_1	246	248	PF00675	0.493
CLV_NRD_NRD_1	380	382	PF00675	0.288
CLV_NRD_NRD_1	407	409	PF00675	0.385
CLV_NRD_NRD_1	691	693	PF00675	0.720
CLV_NRD_NRD_1	694	696	PF00675	0.721
CLV_NRD_NRD_1	8	10	PF00675	0.580
CLV_PCSK_FUR_1	689	693	PF00082	0.623
CLV_PCSK_KEX2_1	13	15	PF00082	0.558
CLV_PCSK_KEX2_1	149	151	PF00082	0.310
CLV_PCSK_KEX2_1	246	248	PF00082	0.522
CLV_PCSK_KEX2_1	407	409	PF00082	0.361
CLV_PCSK_KEX2_1	653	655	PF00082	0.517
CLV_PCSK_KEX2_1	691	693	PF00082	0.720
CLV_PCSK_KEX2_1	694	696	PF00082	0.721
CLV_PCSK_KEX2_1	7	9	PF00082	0.584
CLV_PCSK_PC1ET2_1	653	655	PF00082	0.532
CLV_PCSK_PC7_1	9	15	PF00082	0.711
CLV_PCSK_SKI1_1	105	109	PF00082	0.548
CLV_PCSK_SKI1_1	153	157	PF00082	0.404
CLV_PCSK_SKI1_1	225	229	PF00082	0.385
CLV_PCSK_SKI1_1	286	290	PF00082	0.313
CLV_PCSK_SKI1_1	407	411	PF00082	0.416
CLV_PCSK_SKI1_1	515	519	PF00082	0.559
CLV_PCSK_SKI1_1	557	561	PF00082	0.438
DEG_Nend_UBRbox_1	1	4	PF02207	0.742
DEG_SPOP_SBC_1	339	343	PF00917	0.607
DOC_CKS1_1	409	414	PF01111	0.483
DOC_CYCLIN_yCln2_LP_2	161	164	PF00134	0.409
DOC_MAPK_DCC_7	194	204	PF00069	0.490
DOC_MAPK_FxFP_2	288	291	PF00069	0.406
DOC_MAPK_gen_1	149	158	PF00069	0.366
DOC_MAPK_gen_1	19	29	PF00069	0.365
DOC_MAPK_MEF2A_6	22	31	PF00069	0.328
DOC_MAPK_MEF2A_6	280	289	PF00069	0.306
DOC_MAPK_MEF2A_6	96	103	PF00069	0.315
DOC_PP1_RVXF_1	284	290	PF00149	0.440
DOC_PP2B_LxvP_1	101	104	PF13499	0.350
DOC_PP2B_LxvP_1	161	164	PF13499	0.360
DOC_PP4_FxxP_1	288	291	PF00568	0.346
DOC_USP7_MATH_1	525	529	PF00917	0.407
DOC_USP7_MATH_1	579	583	PF00917	0.747
DOC_USP7_UBL2_3	615	619	PF12436	0.576
DOC_WD40_RPTOR_TOS_1	251	256	PF00400	0.515
DOC_WW_Pin1_4	215	220	PF00397	0.564
DOC_WW_Pin1_4	325	330	PF00397	0.591
DOC_WW_Pin1_4	348	353	PF00397	0.595
DOC_WW_Pin1_4	354	359	PF00397	0.459
DOC_WW_Pin1_4	401	406	PF00397	0.422
DOC_WW_Pin1_4	408	413	PF00397	0.378
DOC_WW_Pin1_4	573	578	PF00397	0.617
DOC_WW_Pin1_4	601	606	PF00397	0.739
DOC_WW_Pin1_4	633	638	PF00397	0.664
LIG_14-3-3_CanoR_1	259	269	PF00244	0.581
LIG_14-3-3_CanoR_1	407	412	PF00244	0.422
LIG_14-3-3_CanoR_1	444	449	PF00244	0.382
LIG_14-3-3_CanoR_1	565	571	PF00244	0.429
LIG_14-3-3_CanoR_1	84	92	PF00244	0.347
LIG_14-3-3_CanoR_1	96	102	PF00244	0.296
LIG_Actin_WH2_2	49	64	PF00022	0.318
LIG_APCC_ABBA_1	304	309	PF00400	0.511
LIG_BIR_III_4	450	454	PF00653	0.421
LIG_BIR_III_4	662	666	PF00653	0.551
LIG_BRCT_BRCA1_1	137	141	PF00533	0.403
LIG_EH1_1	92	100	PF00400	0.297
LIG_FHA_1	112	118	PF00498	0.351
LIG_FHA_1	126	132	PF00498	0.365
LIG_FHA_1	358	364	PF00498	0.439
LIG_FHA_1	408	414	PF00498	0.461
LIG_FHA_1	529	535	PF00498	0.346
LIG_FHA_2	268	274	PF00498	0.592
LIG_FHA_2	310	316	PF00498	0.499
LIG_FHA_2	325	331	PF00498	0.517
LIG_FHA_2	445	451	PF00498	0.469
LIG_FHA_2	499	505	PF00498	0.515
LIG_FHA_2	581	587	PF00498	0.565
LIG_FHA_2	602	608	PF00498	0.667
LIG_FHA_2	634	640	PF00498	0.631
LIG_FHA_2	674	680	PF00498	0.824
LIG_LIR_Apic_2	195	200	PF02991	0.386
LIG_LIR_Gen_1	106	115	PF02991	0.374
LIG_LIR_Gen_1	166	176	PF02991	0.346
LIG_LIR_Gen_1	245	255	PF02991	0.372
LIG_LIR_Gen_1	531	539	PF02991	0.449
LIG_LIR_Gen_1	627	637	PF02991	0.553
LIG_LIR_Nem_3	106	111	PF02991	0.295
LIG_LIR_Nem_3	166	171	PF02991	0.318
LIG_LIR_Nem_3	245	251	PF02991	0.339
LIG_LIR_Nem_3	36	41	PF02991	0.309
LIG_LIR_Nem_3	424	429	PF02991	0.387
LIG_LIR_Nem_3	513	517	PF02991	0.488
LIG_LIR_Nem_3	531	536	PF02991	0.295
LIG_LIR_Nem_3	627	633	PF02991	0.586
LIG_PAM2_1	356	368	PF00658	0.371
LIG_PCNA_yPIPBox_3	144	155	PF02747	0.327
LIG_Pex14_2	648	652	PF04695	0.622
LIG_RPA_C_Fungi	254	266	PF08784	0.595
LIG_SH2_CRK	154	158	PF00017	0.305
LIG_SH2_CRK	159	163	PF00017	0.320
LIG_SH2_CRK	197	201	PF00017	0.368
LIG_SH2_CRK	514	518	PF00017	0.494
LIG_SH2_GRB2like	248	251	PF00017	0.461
LIG_SH2_NCK_1	660	664	PF00017	0.479
LIG_SH2_PTP2	201	204	PF00017	0.417
LIG_SH2_SRC	660	663	PF00017	0.522
LIG_SH2_STAP1	314	318	PF00017	0.468
LIG_SH2_STAP1	429	433	PF00017	0.397
LIG_SH2_STAT3	420	423	PF00017	0.440
LIG_SH2_STAT5	201	204	PF00017	0.380
LIG_SH2_STAT5	418	421	PF00017	0.339
LIG_SH2_STAT5	632	635	PF00017	0.570
LIG_SH2_STAT5	647	650	PF00017	0.470
LIG_SH3_1	320	326	PF00018	0.459
LIG_SH3_2	516	521	PF14604	0.490
LIG_SH3_3	207	213	PF00018	0.589
LIG_SH3_3	263	269	PF00018	0.453
LIG_SH3_3	320	326	PF00018	0.459
LIG_SH3_3	406	412	PF00018	0.376
LIG_SH3_3	513	519	PF00018	0.424
LIG_SH3_3	617	623	PF00018	0.711
LIG_SUMO_SIM_par_1	471	476	PF11976	0.352
LIG_SxIP_EBH_1	135	149	PF03271	0.395
LIG_SxIP_EBH_1	515	528	PF03271	0.447
LIG_TRAF2_1	636	639	PF00917	0.538
LIG_TRFH_1	159	163	PF08558	0.429
LIG_TYR_ITIM	199	204	PF00017	0.465
LIG_UBA3_1	376	382	PF00899	0.301
LIG_UBA3_1	57	62	PF00899	0.347
LIG_WW_3	321	325	PF00397	0.528
MOD_CDC14_SPxK_1	404	407	PF00782	0.409
MOD_CDK_SPxK_1	401	407	PF00069	0.396
MOD_CDK_SPxxK_3	401	408	PF00069	0.440
MOD_CK1_1	240	246	PF00069	0.491
MOD_CK1_1	257	263	PF00069	0.459
MOD_CK1_1	357	363	PF00069	0.527
MOD_CK1_1	528	534	PF00069	0.337
MOD_CK1_1	566	572	PF00069	0.476
MOD_CK1_1	614	620	PF00069	0.722
MOD_CK1_1	85	91	PF00069	0.350
MOD_CK2_1	267	273	PF00069	0.452
MOD_CK2_1	307	313	PF00069	0.452
MOD_CK2_1	324	330	PF00069	0.488
MOD_CK2_1	498	504	PF00069	0.481
MOD_CK2_1	580	586	PF00069	0.537
MOD_CK2_1	633	639	PF00069	0.631
MOD_DYRK1A_RPxSP_1	408	412	PF00069	0.485
MOD_GlcNHglycan	137	140	PF01048	0.567
MOD_GlcNHglycan	259	262	PF01048	0.592
MOD_GlcNHglycan	33	36	PF01048	0.433
MOD_GlcNHglycan	336	339	PF01048	0.724
MOD_GlcNHglycan	616	619	PF01048	0.821
MOD_GSK3_1	215	222	PF00069	0.407
MOD_GSK3_1	325	332	PF00069	0.603
MOD_GSK3_1	334	341	PF00069	0.601
MOD_GSK3_1	343	350	PF00069	0.528
MOD_GSK3_1	354	361	PF00069	0.460
MOD_GSK3_1	494	501	PF00069	0.544
MOD_GSK3_1	524	531	PF00069	0.497
MOD_GSK3_1	563	570	PF00069	0.452
MOD_N-GLC_1	249	254	PF02516	0.348
MOD_N-GLC_1	257	262	PF02516	0.492
MOD_N-GLC_1	358	363	PF02516	0.398
MOD_NEK2_1	135	140	PF00069	0.479
MOD_NEK2_1	237	242	PF00069	0.372
MOD_NEK2_1	421	426	PF00069	0.309
MOD_NEK2_1	494	499	PF00069	0.546
MOD_NEK2_1	82	87	PF00069	0.379
MOD_PIKK_1	192	198	PF00454	0.576
MOD_PKA_1	407	413	PF00069	0.381
MOD_PKA_2	407	413	PF00069	0.380
MOD_PKA_2	564	570	PF00069	0.568
MOD_Plk_1	249	255	PF00069	0.377
MOD_Plk_1	358	364	PF00069	0.456
MOD_Plk_1	429	435	PF00069	0.489
MOD_Plk_1	528	534	PF00069	0.337
MOD_Plk_1	638	644	PF00069	0.515
MOD_Plk_2-3	309	315	PF00069	0.393
MOD_Plk_2-3	673	679	PF00069	0.628
MOD_Plk_4	137	143	PF00069	0.473
MOD_Plk_4	219	225	PF00069	0.552
MOD_Plk_4	371	377	PF00069	0.354
MOD_Plk_4	383	389	PF00069	0.367
MOD_Plk_4	528	534	PF00069	0.382
MOD_Plk_4	647	653	PF00069	0.513
MOD_Plk_4	664	670	PF00069	0.551
MOD_Plk_4	97	103	PF00069	0.428
MOD_ProDKin_1	215	221	PF00069	0.552
MOD_ProDKin_1	325	331	PF00069	0.597
MOD_ProDKin_1	348	354	PF00069	0.584
MOD_ProDKin_1	401	407	PF00069	0.426
MOD_ProDKin_1	408	414	PF00069	0.373
MOD_ProDKin_1	573	579	PF00069	0.624
MOD_ProDKin_1	601	607	PF00069	0.740
MOD_ProDKin_1	633	639	PF00069	0.660
MOD_SUMO_rev_2	221	229	PF00179	0.390
MOD_SUMO_rev_2	510	517	PF00179	0.538
MOD_SUMO_rev_2	586	594	PF00179	0.622
TRG_DiLeu_BaEn_1	301	306	PF01217	0.465
TRG_DiLeu_BaEn_2	370	376	PF01217	0.305
TRG_DiLeu_BaEn_2	528	534	PF01217	0.316
TRG_DiLeu_BaLyEn_6	532	537	PF01217	0.434
TRG_ENDOCYTIC_2	152	155	PF00928	0.284
TRG_ENDOCYTIC_2	159	162	PF00928	0.283
TRG_ENDOCYTIC_2	201	204	PF00928	0.380
TRG_ENDOCYTIC_2	248	251	PF00928	0.461
TRG_ENDOCYTIC_2	418	421	PF00928	0.350
TRG_ENDOCYTIC_2	514	517	PF00928	0.486
TRG_ER_diArg_1	12	14	PF00400	0.568
TRG_ER_diArg_1	149	151	PF00400	0.310
TRG_ER_diArg_1	246	248	PF00400	0.533
TRG_ER_diArg_1	407	409	PF00400	0.385
TRG_ER_diArg_1	654	657	PF00400	0.486
TRG_ER_diArg_1	689	692	PF00400	0.735
TRG_ER_diArg_1	694	696	PF00400	0.698
TRG_ER_diArg_1	7	9	PF00400	0.615
TRG_ER_diArg_1	71	74	PF00400	0.406
TRG_NLS_MonoExtC_3	652	657	PF00514	0.553
TRG_Pf-PMV_PEXEL_1	39	44	PF00026	0.339
TRG_Pf-PMV_PEXEL_1	554	558	PF00026	0.538

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I5J6	Leptomonas seymouri	58%	100%
A0A0S4KL20	Bodo saltans	35%	94%
A0A1X0NSY0	Trypanosomatidae	40%	100%
A0A3Q8ICM8	Leishmania donovani	92%	100%
A0A3R7KTM1	Trypanosoma rangeli	40%	100%
A4HEP8	Leishmania braziliensis	79%	100%
A4I1X8	Leishmania infantum	92%	100%
C9ZS41	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	39%	100%
Q4Q9E9	Leishmania major	91%	100%