Nfu_N domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Scaffold protein Nfu/NifU N terminal/NifU-like domain containing protein, putative

Species:

Leishmania mexicana

UniProt:

E9AY01_LEIMU

TriTrypDb:

LmxM.26.0190

Length:

281

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005739	mitochondrion	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AY01

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AY01

Function

Biological processes
Term	Name	Level	Count
GO:0006790	sulfur compound metabolic process	3	11
GO:0008152	metabolic process	1	11
GO:0009987	cellular process	1	11
GO:0016043	cellular component organization	3	11
GO:0016226	iron-sulfur cluster assembly	4	11
GO:0022607	cellular component assembly	4	11
GO:0031163	metallo-sulfur cluster assembly	5	11
GO:0044237	cellular metabolic process	2	11
GO:0071840	cellular component organization or biogenesis	2	11
GO:0006807	nitrogen compound metabolic process	2	1
GO:0019538	protein metabolic process	3	1
GO:0043170	macromolecule metabolic process	3	1
GO:0044238	primary metabolic process	2	1
GO:0051604	protein maturation	4	1
GO:0071704	organic substance metabolic process	2	1
GO:0097428	protein maturation by iron-sulfur cluster transfer	5	1
GO:1901564	organonitrogen compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	11
GO:0005506	iron ion binding	6	11
GO:0043167	ion binding	2	11
GO:0043169	cation binding	3	11
GO:0046872	metal ion binding	4	11
GO:0046914	transition metal ion binding	5	11
GO:0051536	iron-sulfur cluster binding	3	11
GO:0051540	metal cluster binding	2	11
GO:0051539	4 iron, 4 sulfur cluster binding	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	127	129	PF00675	0.289
CLV_NRD_NRD_1	267	269	PF00675	0.433
CLV_NRD_NRD_1	3	5	PF00675	0.591
CLV_NRD_NRD_1	33	35	PF00675	0.482
CLV_PCSK_KEX2_1	265	267	PF00082	0.396
CLV_PCSK_KEX2_1	3	5	PF00082	0.573
CLV_PCSK_KEX2_1	33	35	PF00082	0.482
CLV_PCSK_PC1ET2_1	265	267	PF00082	0.481
CLV_PCSK_SKI1_1	15	19	PF00082	0.565
CLV_PCSK_SKI1_1	186	190	PF00082	0.213
CLV_PCSK_SKI1_1	194	198	PF00082	0.235
CLV_PCSK_SKI1_1	269	273	PF00082	0.519
CLV_PCSK_SKI1_1	34	38	PF00082	0.581
DEG_APCC_KENBOX_2	152	156	PF00400	0.436
DEG_Nend_Nbox_1	1	3	PF02207	0.446
DEG_SPOP_SBC_1	27	31	PF00917	0.645
DOC_CYCLIN_RxL_1	183	191	PF00134	0.244
DOC_MAPK_MEF2A_6	77	85	PF00069	0.223
DOC_MAPK_RevD_3	113	129	PF00069	0.323
DOC_PP4_FxxP_1	85	88	PF00568	0.273
DOC_USP7_MATH_1	16	20	PF00917	0.524
DOC_USP7_MATH_1	221	225	PF00917	0.366
DOC_USP7_MATH_1	27	31	PF00917	0.631
DOC_USP7_UBL2_3	265	269	PF12436	0.464
DOC_WW_Pin1_4	100	105	PF00397	0.219
DOC_WW_Pin1_4	48	53	PF00397	0.494
DOC_WW_Pin1_4	67	72	PF00397	0.263
DOC_WW_Pin1_4	93	98	PF00397	0.282
LIG_14-3-3_CanoR_1	15	22	PF00244	0.543
LIG_14-3-3_CanoR_1	33	37	PF00244	0.485
LIG_14-3-3_CanoR_1	4	10	PF00244	0.505
LIG_14-3-3_CanoR_1	77	82	PF00244	0.260
LIG_BRCT_BRCA1_1	44	48	PF00533	0.364
LIG_FHA_1	211	217	PF00498	0.422
LIG_FHA_1	35	41	PF00498	0.685
LIG_FHA_2	170	176	PF00498	0.461
LIG_LIR_Apic_2	82	88	PF02991	0.244
LIG_LIR_Gen_1	122	131	PF02991	0.253
LIG_LIR_Gen_1	133	142	PF02991	0.228
LIG_LIR_Gen_1	213	221	PF02991	0.420
LIG_LIR_Nem_3	122	127	PF02991	0.244
LIG_LIR_Nem_3	133	138	PF02991	0.244
LIG_LIR_Nem_3	213	218	PF02991	0.443
LIG_SH2_CRK	101	105	PF00017	0.262
LIG_SH2_PTP2	124	127	PF00017	0.244
LIG_SH2_STAP1	12	16	PF00017	0.407
LIG_SH2_STAT5	124	127	PF00017	0.258
LIG_SH3_3	89	95	PF00018	0.204
LIG_SUMO_SIM_anti_2	39	45	PF11976	0.431
LIG_SUMO_SIM_par_1	176	187	PF11976	0.359
LIG_SUMO_SIM_par_1	39	45	PF11976	0.434
LIG_TRAF2_1	160	163	PF00917	0.490
LIG_UBA3_1	138	143	PF00899	0.300
MOD_CK1_1	224	230	PF00069	0.403
MOD_CK1_1	5	11	PF00069	0.454
MOD_CK1_1	51	57	PF00069	0.521
MOD_CK2_1	231	237	PF00069	0.501
MOD_GlcNHglycan	157	160	PF01048	0.420
MOD_GlcNHglycan	18	21	PF01048	0.460
MOD_GlcNHglycan	233	236	PF01048	0.230
MOD_GlcNHglycan	4	7	PF01048	0.525
MOD_GlcNHglycan	44	47	PF01048	0.507
MOD_GlcNHglycan	48	51	PF01048	0.483
MOD_GlcNHglycan	53	56	PF01048	0.373
MOD_GSK3_1	16	23	PF00069	0.485
MOD_GSK3_1	221	228	PF00069	0.409
MOD_GSK3_1	28	35	PF00069	0.575
MOD_GSK3_1	42	49	PF00069	0.598
MOD_GSK3_1	77	84	PF00069	0.244
MOD_N-GLC_1	169	174	PF02516	0.434
MOD_N-GLC_1	67	72	PF02516	0.323
MOD_NEK2_1	188	193	PF00069	0.445
MOD_NEK2_1	2	7	PF00069	0.508
MOD_NEK2_1	36	41	PF00069	0.538
MOD_NEK2_1	81	86	PF00069	0.248
MOD_NEK2_2	28	33	PF00069	0.442
MOD_PKA_2	2	8	PF00069	0.527
MOD_PKA_2	32	38	PF00069	0.522
MOD_Plk_1	81	87	PF00069	0.244
MOD_Plk_1	88	94	PF00069	0.244
MOD_Plk_4	210	216	PF00069	0.424
MOD_ProDKin_1	100	106	PF00069	0.219
MOD_ProDKin_1	48	54	PF00069	0.491
MOD_ProDKin_1	67	73	PF00069	0.263
MOD_ProDKin_1	93	99	PF00069	0.282
MOD_SUMO_for_1	86	89	PF00179	0.375
TRG_DiLeu_BaEn_2	210	216	PF01217	0.461
TRG_ENDOCYTIC_2	124	127	PF00928	0.244
TRG_ER_diArg_1	192	195	PF00400	0.407
TRG_ER_diArg_1	2	4	PF00400	0.594
TRG_ER_diArg_1	32	34	PF00400	0.488
TRG_Pf-PMV_PEXEL_1	269	273	PF00026	0.500

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I2Q0	Leptomonas seymouri	79%	96%
A0A0N1I735	Leptomonas seymouri	31%	100%
A0A0S4IJH0	Bodo saltans	56%	100%
A0A0S4IVB5	Bodo saltans	35%	100%
A0A1X0NST9	Trypanosomatidae	69%	99%
A0A1X0P8F8	Trypanosomatidae	30%	100%
A0A3R7MX73	Trypanosoma rangeli	58%	100%
A0A3S5H7F5	Leishmania donovani	91%	99%
A0A3S7XAI2	Leishmania donovani	33%	94%
A4HEM5	Leishmania braziliensis	81%	100%
A4HNU8	Leishmania braziliensis	32%	100%
A4I1V6	Leishmania infantum	91%	99%
A4ICP6	Leishmania infantum	33%	94%
B3MRT7	Drosophila ananassae	40%	98%
B3NYF7	Drosophila erecta	41%	99%
B4H303	Drosophila persimilis	40%	100%
B4IMF6	Drosophila sechellia	40%	99%
B4JWR9	Drosophila grimshawi	40%	94%
B4M375	Drosophila virilis	40%	94%
B4NE93	Drosophila willistoni	41%	97%
B4PZ52	Drosophila yakuba	40%	99%
B4R3T1	Drosophila simulans	40%	99%
B5DKJ8	Drosophila pseudoobscura pseudoobscura	40%	98%
C9ZS65	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	68%	100%
D0A2R6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	35%	100%
E9ASK8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	93%
P32860	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	35%	100%
Q4Q1Y8	Leishmania major	33%	90%
Q4Q9H2	Leishmania major	93%	100%
Q8SY96	Drosophila melanogaster	41%	99%
Q9C8J2	Arabidopsis thaliana	41%	100%
Q9LIG6	Arabidopsis thaliana	38%	99%
Q9QZ23	Mus musculus	41%	100%
Q9UMS0	Homo sapiens	42%	100%
Q9UUB8	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	40%	100%
V5DRN0	Trypanosoma cruzi	33%	100%