Putative 3-oxo-5-alpha-steroid 4-dehydrogenase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

3-oxo-5-alpha-steroid 4-dehydrogenase, putative

Species:

Leishmania mexicana

UniProt:

E9AXQ9_LEIMU

TriTrypDb:

LmxM.25.1770

Length:

306

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 18

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	11
GO:0110165	cellular anatomical entity	1	11
GO:0005737	cytoplasm	2	1
GO:0005777	peroxisome	6	1
GO:0020015	glycosome	7	1
GO:0042579	microbody	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0005789	endoplasmic reticulum membrane	4	1
GO:0031090	organelle membrane	3	1

Expansion

Sequence features

E9AXQ9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AXQ9

Function

Biological processes
Term	Name	Level	Count
GO:0006629	lipid metabolic process	3	11
GO:0008152	metabolic process	1	11
GO:0044238	primary metabolic process	2	11
GO:0071704	organic substance metabolic process	2	11
GO:0000038	very long-chain fatty acid metabolic process	5	1
GO:0006082	organic acid metabolic process	3	1
GO:0006631	fatty acid metabolic process	4	1
GO:0006633	fatty acid biosynthetic process	5	1
GO:0008610	lipid biosynthetic process	4	1
GO:0009058	biosynthetic process	2	1
GO:0009987	cellular process	1	1
GO:0016053	organic acid biosynthetic process	4	1
GO:0019752	carboxylic acid metabolic process	5	1
GO:0032787	monocarboxylic acid metabolic process	6	1
GO:0042761	very long-chain fatty acid biosynthetic process	6	1
GO:0043436	oxoacid metabolic process	4	1
GO:0044237	cellular metabolic process	2	1
GO:0044249	cellular biosynthetic process	3	1
GO:0044255	cellular lipid metabolic process	3	1
GO:0044281	small molecule metabolic process	2	1
GO:0044283	small molecule biosynthetic process	3	1
GO:0046394	carboxylic acid biosynthetic process	5	1
GO:0072330	monocarboxylic acid biosynthetic process	6	1
GO:1901576	organic substance biosynthetic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0016491	oxidoreductase activity	2	11
GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors	3	11
GO:0003865	3-oxo-5-alpha-steroid 4-dehydrogenase activity	5	4
GO:0016229	steroid dehydrogenase activity	3	4
GO:0033765	steroid dehydrogenase activity, acting on the CH-CH group of donors	4	4
GO:0016628	oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor	4	1
GO:0102758	very-long-chain enoyl-CoA reductase activity	5	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	7	11	PF00656	0.640
CLV_NRD_NRD_1	12	14	PF00675	0.442
CLV_NRD_NRD_1	222	224	PF00675	0.473
CLV_NRD_NRD_1	39	41	PF00675	0.315
CLV_PCSK_KEX2_1	146	148	PF00082	0.277
CLV_PCSK_PC1ET2_1	146	148	PF00082	0.277
CLV_PCSK_SKI1_1	165	169	PF00082	0.362
CLV_PCSK_SKI1_1	41	45	PF00082	0.312
CLV_PCSK_SKI1_1	96	100	PF00082	0.259
DEG_APCC_DBOX_1	60	68	PF00400	0.639
DEG_Nend_UBRbox_1	1	4	PF02207	0.504
DOC_MAPK_gen_1	13	19	PF00069	0.614
DOC_MAPK_gen_1	146	154	PF00069	0.506
DOC_MAPK_gen_1	296	304	PF00069	0.469
DOC_MAPK_gen_1	59	67	PF00069	0.624
DOC_MAPK_MEF2A_6	134	141	PF00069	0.424
DOC_MAPK_MEF2A_6	29	38	PF00069	0.548
DOC_MAPK_NFAT4_5	134	142	PF00069	0.424
DOC_PP4_FxxP_1	157	160	PF00568	0.536
DOC_PP4_FxxP_1	43	46	PF00568	0.632
DOC_USP7_MATH_1	195	199	PF00917	0.351
DOC_USP7_MATH_1	240	244	PF00917	0.321
DOC_WW_Pin1_4	47	52	PF00397	0.654
LIG_14-3-3_CanoR_1	40	44	PF00244	0.510
LIG_APCC_ABBA_1	83	88	PF00400	0.488
LIG_BRCT_BRCA1_1	264	268	PF00533	0.314
LIG_FHA_1	130	136	PF00498	0.322
LIG_FHA_1	228	234	PF00498	0.273
LIG_FHA_1	259	265	PF00498	0.354
LIG_FHA_2	22	28	PF00498	0.529
LIG_FHA_2	64	70	PF00498	0.653
LIG_GBD_Chelix_1	107	115	PF00786	0.424
LIG_GBD_Chelix_1	141	149	PF00786	0.298
LIG_LIR_Apic_2	102	106	PF02991	0.312
LIG_LIR_Apic_2	42	46	PF02991	0.629
LIG_LIR_Gen_1	176	185	PF02991	0.331
LIG_LIR_Gen_1	253	259	PF02991	0.326
LIG_LIR_Nem_3	176	180	PF02991	0.334
LIG_LIR_Nem_3	198	203	PF02991	0.374
LIG_LIR_Nem_3	253	257	PF02991	0.326
LIG_LIR_Nem_3	265	271	PF02991	0.351
LIG_LIR_Nem_3	97	103	PF02991	0.378
LIG_MLH1_MIPbox_1	265	269	PF16413	0.401
LIG_PALB2_WD40_1	263	271	PF16756	0.340
LIG_Pex14_2	140	144	PF04695	0.326
LIG_Pex14_2	153	157	PF04695	0.543
LIG_PTB_Apo_2	167	174	PF02174	0.459
LIG_PTB_Apo_2	184	191	PF02174	0.165
LIG_PTB_Phospho_1	167	173	PF10480	0.459
LIG_SH2_CRK	103	107	PF00017	0.312
LIG_SH2_CRK	95	99	PF00017	0.459
LIG_SH2_PTP2	136	139	PF00017	0.385
LIG_SH2_SRC	32	35	PF00017	0.586
LIG_SH2_STAP1	131	135	PF00017	0.331
LIG_SH2_STAT5	100	103	PF00017	0.456
LIG_SH2_STAT5	131	134	PF00017	0.339
LIG_SH2_STAT5	136	139	PF00017	0.327
LIG_SH2_STAT5	183	186	PF00017	0.325
LIG_SH2_STAT5	247	250	PF00017	0.259
LIG_SH2_STAT5	288	291	PF00017	0.473
LIG_SH3_1	231	237	PF00018	0.289
LIG_SH3_2	160	165	PF14604	0.489
LIG_SH3_3	157	163	PF00018	0.523
LIG_SH3_3	231	237	PF00018	0.263
LIG_SH3_3	239	245	PF00018	0.218
LIG_SUMO_SIM_par_1	62	69	PF11976	0.578
LIG_TYR_ITIM	84	89	PF00017	0.522
LIG_TYR_ITIM	93	98	PF00017	0.479
LIG_WRC_WIRS_1	174	179	PF05994	0.312
MOD_CK1_1	158	164	PF00069	0.447
MOD_CK1_1	189	195	PF00069	0.154
MOD_CK1_1	253	259	PF00069	0.372
MOD_CK1_1	47	53	PF00069	0.689
MOD_GlcNHglycan	122	125	PF01048	0.598
MOD_GlcNHglycan	193	196	PF01048	0.498
MOD_GlcNHglycan	21	24	PF01048	0.355
MOD_GlcNHglycan	6	9	PF01048	0.371
MOD_GSK3_1	186	193	PF00069	0.339
MOD_GSK3_1	201	208	PF00069	0.305
MOD_GSK3_1	218	225	PF00069	0.337
MOD_GSK3_1	258	265	PF00069	0.325
MOD_GSK3_1	59	66	PF00069	0.541
MOD_NEK2_1	19	24	PF00069	0.508
MOD_NEK2_1	190	195	PF00069	0.308
MOD_NEK2_1	263	268	PF00069	0.323
MOD_NEK2_2	94	99	PF00069	0.497
MOD_PIKK_1	129	135	PF00454	0.320
MOD_PIKK_1	193	199	PF00454	0.154
MOD_PIKK_1	71	77	PF00454	0.588
MOD_PK_1	79	85	PF00069	0.568
MOD_PKA_2	115	121	PF00069	0.308
MOD_PKA_2	222	228	PF00069	0.362
MOD_PKA_2	39	45	PF00069	0.536
MOD_Plk_4	115	121	PF00069	0.308
MOD_Plk_4	148	154	PF00069	0.517
MOD_Plk_4	201	207	PF00069	0.258
MOD_Plk_4	250	256	PF00069	0.274
MOD_Plk_4	263	269	PF00069	0.270
MOD_Plk_4	79	85	PF00069	0.617
MOD_ProDKin_1	47	53	PF00069	0.657
MOD_SUMO_for_1	145	148	PF00179	0.485
MOD_SUMO_rev_2	22	31	PF00179	0.532
TRG_ENDOCYTIC_2	136	139	PF00928	0.385
TRG_ENDOCYTIC_2	174	177	PF00928	0.312
TRG_ENDOCYTIC_2	86	89	PF00928	0.527
TRG_ENDOCYTIC_2	95	98	PF00928	0.481
TRG_NLS_Bipartite_1	282	300	PF00514	0.491
TRG_NLS_MonoExtC_3	281	286	PF00514	0.539
TRG_NLS_MonoExtN_4	282	287	PF00514	0.525
TRG_NLS_MonoExtN_4	293	300	PF00514	0.485

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PF75	Leptomonas seymouri	72%	99%
A0A0S4IKY4	Bodo saltans	57%	100%
A0A1X0NYG0	Trypanosomatidae	59%	100%
A0A3Q8INK5	Leishmania donovani	94%	100%
A0A422NGL4	Trypanosoma rangeli	60%	100%
A4HEG5	Leishmania braziliensis	86%	100%
A4I1M5	Leishmania infantum	94%	100%
C9ZK67	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	62%	100%
O94511	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	37%	100%
P24008	Rattus norvegicus	23%	100%
Q3SZ89	Bos taurus	29%	84%
Q3ZCD7	Bos taurus	36%	99%
Q4Q9R2	Leishmania major	93%	100%
Q55C17	Dictyostelium discoideum	38%	100%
Q5HYJ1	Homo sapiens	30%	84%
Q64232	Rattus norvegicus	39%	99%
Q68FF9	Mus musculus	24%	100%
Q8BFZ1	Mus musculus	30%	85%
Q99190	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	29%	99%
Q9CY27	Mus musculus	39%	99%
Q9M2U2	Arabidopsis thaliana	35%	99%
Q9N5Y2	Caenorhabditis elegans	39%	99%
Q9NZ01	Homo sapiens	36%	99%
Q9UT20	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	27%	100%