Putative rev7 (Putative mitotic spindle checkpoint component)

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Mad2-like HORMA domain containing protein

Species:

Leishmania mexicana

UniProt:

E9AXP8_LEIMU

TriTrypDb:

LmxM.25.1670

Length:

201

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	9
GO:0043226	organelle	2	10
GO:0043227	membrane-bounded organelle	3	9
GO:0043229	intracellular organelle	3	10
GO:0043231	intracellular membrane-bounded organelle	4	9
GO:0110165	cellular anatomical entity	1	10
GO:0000776	kinetochore	3	1
GO:0005654	nucleoplasm	2	1
GO:0005737	cytoplasm	2	1
GO:0005930	axoneme	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0099080	supramolecular complex	2	1

Expansion

Sequence features

E9AXP8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AXP8

Function

Biological processes
Term	Name	Level	Count
GO:0000075	cell cycle checkpoint signaling	4	10
GO:0007088	regulation of mitotic nuclear division	6	10
GO:0007093	mitotic cell cycle checkpoint signaling	4	10
GO:0007094	mitotic spindle assembly checkpoint signaling	6	10
GO:0007165	signal transduction	2	10
GO:0007346	regulation of mitotic cell cycle	5	10
GO:0009987	cellular process	1	10
GO:0010564	regulation of cell cycle process	5	10
GO:0010639	negative regulation of organelle organization	6	10
GO:0010948	negative regulation of cell cycle process	6	10
GO:0010965	regulation of mitotic sister chromatid separation	6	10
GO:0022402	cell cycle process	2	10
GO:0030071	regulation of mitotic metaphase/anaphase transition	7	10
GO:0031577	spindle checkpoint signaling	5	10
GO:0033043	regulation of organelle organization	5	10
GO:0033044	regulation of chromosome organization	6	10
GO:0033045	regulation of sister chromatid segregation	5	10
GO:0033046	negative regulation of sister chromatid segregation	6	10
GO:0033047	regulation of mitotic sister chromatid segregation	6	10
GO:0033048	negative regulation of mitotic sister chromatid segregation	7	10
GO:0035556	intracellular signal transduction	3	10
GO:0045786	negative regulation of cell cycle	5	10
GO:0045839	negative regulation of mitotic nuclear division	7	10
GO:0045841	negative regulation of mitotic metaphase/anaphase transition	8	10
GO:0045930	negative regulation of mitotic cell cycle	6	10
GO:0048519	negative regulation of biological process	3	10
GO:0048523	negative regulation of cellular process	4	10
GO:0050789	regulation of biological process	2	10
GO:0050794	regulation of cellular process	3	10
GO:0051128	regulation of cellular component organization	4	10
GO:0051129	negative regulation of cellular component organization	5	10
GO:0051726	regulation of cell cycle	4	10
GO:0051783	regulation of nuclear division	6	10
GO:0051784	negative regulation of nuclear division	7	10
GO:0051983	regulation of chromosome segregation	4	10
GO:0051985	negative regulation of chromosome segregation	5	10
GO:0065007	biological regulation	1	10
GO:0071173	spindle assembly checkpoint signaling	6	10
GO:0071174	mitotic spindle checkpoint signaling	5	10
GO:1901987	regulation of cell cycle phase transition	6	10
GO:1901988	negative regulation of cell cycle phase transition	7	10
GO:1901990	regulation of mitotic cell cycle phase transition	6	10
GO:1901991	negative regulation of mitotic cell cycle phase transition	7	10
GO:1902099	regulation of metaphase/anaphase transition of cell cycle	6	10
GO:1902100	negative regulation of metaphase/anaphase transition of cell cycle	7	10
GO:1903047	mitotic cell cycle process	3	10
GO:1905818	regulation of chromosome separation	5	10
GO:1905819	negative regulation of chromosome separation	6	10
GO:2000816	negative regulation of mitotic sister chromatid separation	7	10
GO:2001251	negative regulation of chromosome organization	7	10

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	156	160	PF00656	0.351
CLV_NRD_NRD_1	116	118	PF00675	0.322
CLV_NRD_NRD_1	79	81	PF00675	0.259
CLV_PCSK_KEX2_1	176	178	PF00082	0.223
CLV_PCSK_KEX2_1	79	81	PF00082	0.372
CLV_PCSK_PC1ET2_1	176	178	PF00082	0.223
CLV_PCSK_SKI1_1	132	136	PF00082	0.287
CLV_PCSK_SKI1_1	184	188	PF00082	0.382
DEG_SPOP_SBC_1	106	110	PF00917	0.284
DOC_MAPK_gen_1	176	183	PF00069	0.320
DOC_MAPK_gen_1	44	52	PF00069	0.322
DOC_MAPK_gen_1	79	85	PF00069	0.262
DOC_MAPK_MEF2A_6	44	52	PF00069	0.259
DOC_MAPK_NFAT4_5	45	53	PF00069	0.372
DOC_USP7_MATH_1	105	109	PF00917	0.456
DOC_USP7_MATH_1	116	120	PF00917	0.307
DOC_USP7_MATH_1	136	140	PF00917	0.308
LIG_14-3-3_CanoR_1	117	125	PF00244	0.342
LIG_14-3-3_CanoR_1	132	141	PF00244	0.182
LIG_14-3-3_CanoR_1	184	193	PF00244	0.260
LIG_EH1_1	22	30	PF00400	0.372
LIG_EVH1_2	36	40	PF00568	0.351
LIG_FHA_1	133	139	PF00498	0.283
LIG_FHA_1	16	22	PF00498	0.195
LIG_FHA_1	193	199	PF00498	0.654
LIG_FHA_1	3	9	PF00498	0.587
LIG_FHA_2	14	20	PF00498	0.280
LIG_FHA_2	154	160	PF00498	0.271
LIG_FHA_2	163	169	PF00498	0.296
LIG_FHA_2	52	58	PF00498	0.251
LIG_IRF3_LxIS_1	46	53	PF10401	0.325
LIG_LIR_Gen_1	187	198	PF02991	0.547
LIG_LIR_Gen_1	38	48	PF02991	0.323
LIG_LIR_Nem_3	139	143	PF02991	0.293
LIG_LIR_Nem_3	18	23	PF02991	0.256
LIG_LIR_Nem_3	187	193	PF02991	0.282
LIG_LIR_Nem_3	38	43	PF02991	0.139
LIG_NRBOX	61	67	PF00104	0.316
LIG_Pex14_2	186	190	PF04695	0.316
LIG_PTB_Apo_2	24	31	PF02174	0.372
LIG_PTB_Phospho_1	24	30	PF10480	0.372
LIG_SH2_NCK_1	155	159	PF00017	0.372
LIG_SH2_STAT5	155	158	PF00017	0.327
LIG_SH2_STAT5	20	23	PF00017	0.330
LIG_SH2_STAT5	61	64	PF00017	0.285
LIG_SH3_3	166	172	PF00018	0.393
LIG_SH3_3	31	37	PF00018	0.365
LIG_SUMO_SIM_par_1	80	87	PF11976	0.318
LIG_WRC_WIRS_1	183	188	PF05994	0.256
MOD_CK1_1	13	19	PF00069	0.319
MOD_CK1_1	185	191	PF00069	0.289
MOD_CK1_1	90	96	PF00069	0.405
MOD_CK2_1	13	19	PF00069	0.280
MOD_CK2_1	162	168	PF00069	0.372
MOD_GlcNHglycan	113	116	PF01048	0.348
MOD_GlcNHglycan	156	159	PF01048	0.389
MOD_GSK3_1	107	114	PF00069	0.249
MOD_GSK3_1	132	139	PF00069	0.251
MOD_GSK3_1	182	189	PF00069	0.330
MOD_GSK3_1	2	9	PF00069	0.540
MOD_GSK3_1	46	53	PF00069	0.332
MOD_GSK3_1	83	90	PF00069	0.372
MOD_NEK2_1	1	6	PF00069	0.550
MOD_NEK2_1	10	15	PF00069	0.500
MOD_NEK2_1	153	158	PF00069	0.372
MOD_NEK2_1	186	191	PF00069	0.316
MOD_NEK2_1	46	51	PF00069	0.441
MOD_NEK2_2	74	79	PF00069	0.332
MOD_PIKK_1	142	148	PF00454	0.332
MOD_PKA_1	176	182	PF00069	0.223
MOD_PKA_2	116	122	PF00069	0.332
MOD_PKA_2	176	182	PF00069	0.284
MOD_Plk_1	193	199	PF00069	0.553
MOD_Plk_4	136	142	PF00069	0.280
MOD_Plk_4	193	199	PF00069	0.559
MOD_Plk_4	46	52	PF00069	0.443
MOD_Plk_4	68	74	PF00069	0.343
TRG_ENDOCYTIC_2	20	23	PF00928	0.277
TRG_ER_diArg_1	79	81	PF00400	0.372

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I9P7	Leptomonas seymouri	74%	100%
A0A1X0NYH2	Trypanosomatidae	55%	100%
A0A3Q8IGN4	Leishmania donovani	97%	100%
A0A3R7NLU2	Trypanosoma rangeli	50%	100%
A4HEH6	Leishmania braziliensis	88%	100%
A4I1L4	Leishmania infantum	97%	100%
C9ZK57	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	48%	100%
D3Z8D9	Rattus norvegicus	28%	95%
O14417	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	40%	99%
P40958	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	38%	100%
Q13257	Homo sapiens	42%	98%
Q28H85	Xenopus tropicalis	28%	95%
Q2KIP7	Bos taurus	28%	95%
Q4KWZ6	Gallus gallus	29%	95%
Q4Q9S3	Leishmania major	95%	100%
Q556Y9	Dictyostelium discoideum	44%	99%
Q568H3	Danio rerio	28%	95%
Q59VQ3	Candida albicans (strain SC5314 / ATCC MYA-2876)	37%	94%
Q8QFR4	Xenopus laevis	28%	95%
Q9D752	Mus musculus	28%	95%
Q9LU93	Arabidopsis thaliana	39%	96%
Q9UI95	Homo sapiens	29%	95%
Q9XFH3	Zea mays	40%	97%
Q9Z1B5	Mus musculus	42%	98%