Calpain family cysteine protease-like protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

calpain family cysteine protease-like protein

Species:

Leishmania mexicana

UniProt:

E9AXM9_LEIMU

TriTrypDb:

LmxM.25.1480

Length:

705

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 1
Forrest at al. (metacyclic)	no	yes: 3
Forrest at al. (procyclic)	no	yes: 4
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 36

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 16

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 20

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 1, no: 93
NetGPI	no	yes: 0, no: 94

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	1
GO:0110165	cellular anatomical entity	1	5
GO:0005737	cytoplasm	2	3
GO:0005930	axoneme	2	1
GO:0005815	microtubule organizing center	2	1
GO:0005856	cytoskeleton	5	1
GO:0020016	ciliary pocket	2	1
GO:0020038	subpellicular network	2	1
GO:0030863	cortical cytoskeleton	6	1
GO:0036064	ciliary basal body	3	1
GO:0043226	organelle	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1

Expansion

Sequence features

E9AXM9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AXM9

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	95
GO:0006807	nitrogen compound metabolic process	2	95
GO:0008152	metabolic process	1	95
GO:0019538	protein metabolic process	3	95
GO:0043170	macromolecule metabolic process	3	95
GO:0044238	primary metabolic process	2	95
GO:0071704	organic substance metabolic process	2	95
GO:1901564	organonitrogen compound metabolic process	3	95

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	95
GO:0004175	endopeptidase activity	4	95
GO:0004197	cysteine-type endopeptidase activity	5	95
GO:0004198	calcium-dependent cysteine-type endopeptidase activity	6	95
GO:0008233	peptidase activity	3	95
GO:0008234	cysteine-type peptidase activity	4	95
GO:0016787	hydrolase activity	2	95
GO:0140096	catalytic activity, acting on a protein	2	95

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	184	188	PF00656	0.518
CLV_NRD_NRD_1	138	140	PF00675	0.543
CLV_NRD_NRD_1	159	161	PF00675	0.533
CLV_NRD_NRD_1	199	201	PF00675	0.340
CLV_NRD_NRD_1	493	495	PF00675	0.559
CLV_NRD_NRD_1	603	605	PF00675	0.399
CLV_NRD_NRD_1	611	613	PF00675	0.371
CLV_NRD_NRD_1	75	77	PF00675	0.487
CLV_PCSK_KEX2_1	159	161	PF00082	0.343
CLV_PCSK_KEX2_1	191	193	PF00082	0.329
CLV_PCSK_KEX2_1	199	201	PF00082	0.331
CLV_PCSK_KEX2_1	493	495	PF00082	0.487
CLV_PCSK_KEX2_1	611	613	PF00082	0.448
CLV_PCSK_PC1ET2_1	191	193	PF00082	0.373
CLV_PCSK_SKI1_1	153	157	PF00082	0.508
CLV_PCSK_SKI1_1	249	253	PF00082	0.303
CLV_PCSK_SKI1_1	34	38	PF00082	0.327
CLV_PCSK_SKI1_1	362	366	PF00082	0.286
CLV_PCSK_SKI1_1	385	389	PF00082	0.201
CLV_PCSK_SKI1_1	611	615	PF00082	0.497
CLV_Separin_Metazoa	73	77	PF03568	0.203
DEG_MDM2_SWIB_1	474	481	PF02201	0.495
DEG_SCF_TRCP1_1	79	84	PF00400	0.208
DOC_CKS1_1	380	385	PF01111	0.506
DOC_CYCLIN_RxL_1	359	367	PF00134	0.583
DOC_MAPK_gen_1	122	131	PF00069	0.357
DOC_MAPK_gen_1	207	215	PF00069	0.613
DOC_MAPK_gen_1	374	380	PF00069	0.485
DOC_MAPK_gen_1	599	609	PF00069	0.536
DOC_MAPK_gen_1	623	630	PF00069	0.425
DOC_MAPK_MEF2A_6	207	215	PF00069	0.573
DOC_MAPK_MEF2A_6	551	559	PF00069	0.368
DOC_PP2B_LxvP_1	607	610	PF13499	0.395
DOC_USP7_MATH_1	20	24	PF00917	0.398
DOC_USP7_MATH_1	320	324	PF00917	0.472
DOC_USP7_MATH_1	436	440	PF00917	0.553
DOC_USP7_MATH_1	573	577	PF00917	0.477
DOC_USP7_MATH_1	85	89	PF00917	0.409
DOC_USP7_UBL2_3	12	16	PF12436	0.497
DOC_USP7_UBL2_3	122	126	PF12436	0.491
DOC_USP7_UBL2_3	140	144	PF12436	0.590
DOC_USP7_UBL2_3	208	212	PF12436	0.401
DOC_USP7_UBL2_3	249	253	PF12436	0.629
DOC_USP7_UBL2_3	315	319	PF12436	0.505
DOC_USP7_UBL2_3	385	389	PF12436	0.401
DOC_USP7_UBL2_3	95	99	PF12436	0.426
DOC_WW_Pin1_4	217	222	PF00397	0.489
DOC_WW_Pin1_4	24	29	PF00397	0.389
DOC_WW_Pin1_4	379	384	PF00397	0.519
DOC_WW_Pin1_4	448	453	PF00397	0.527
LIG_14-3-3_CanoR_1	592	597	PF00244	0.443
LIG_14-3-3_CanoR_1	611	621	PF00244	0.474
LIG_Actin_WH2_2	373	391	PF00022	0.574
LIG_BRCT_BRCA1_1	481	485	PF00533	0.503
LIG_BRCT_BRCA1_1	488	492	PF00533	0.487
LIG_CNOT1_NIM_1	498	507	PF04054	0.253
LIG_deltaCOP1_diTrp_1	307	312	PF00928	0.575
LIG_deltaCOP1_diTrp_1	42	46	PF00928	0.279
LIG_deltaCOP1_diTrp_1	442	447	PF00928	0.502
LIG_deltaCOP1_diTrp_1	477	485	PF00928	0.514
LIG_FHA_1	280	286	PF00498	0.517
LIG_FHA_1	515	521	PF00498	0.364
LIG_FHA_1	582	588	PF00498	0.576
LIG_FHA_1	70	76	PF00498	0.460
LIG_FHA_1	82	88	PF00498	0.374
LIG_FHA_2	114	120	PF00498	0.496
LIG_FHA_2	5	11	PF00498	0.488
LIG_IRF3_LxIS_1	272	277	PF10401	0.405
LIG_LIR_Apic_2	195	201	PF02991	0.501
LIG_LIR_Gen_1	307	317	PF02991	0.498
LIG_LIR_Gen_1	323	332	PF02991	0.510
LIG_LIR_Gen_1	50	60	PF02991	0.286
LIG_LIR_Gen_1	509	518	PF02991	0.400
LIG_LIR_Gen_1	549	559	PF02991	0.409
LIG_LIR_Gen_1	700	705	PF02991	0.618
LIG_LIR_LC3C_4	700	705	PF02991	0.354
LIG_LIR_Nem_3	277	283	PF02991	0.496
LIG_LIR_Nem_3	307	312	PF02991	0.502
LIG_LIR_Nem_3	323	327	PF02991	0.503
LIG_LIR_Nem_3	340	345	PF02991	0.529
LIG_LIR_Nem_3	42	47	PF02991	0.338
LIG_LIR_Nem_3	451	457	PF02991	0.472
LIG_LIR_Nem_3	477	481	PF02991	0.475
LIG_LIR_Nem_3	50	56	PF02991	0.349
LIG_LIR_Nem_3	500	504	PF02991	0.425
LIG_LIR_Nem_3	509	513	PF02991	0.386
LIG_LIR_Nem_3	526	532	PF02991	0.475
LIG_LIR_Nem_3	549	555	PF02991	0.432
LIG_LIR_Nem_3	595	600	PF02991	0.387
LIG_LIR_Nem_3	700	704	PF02991	0.566
LIG_LYPXL_yS_3	457	460	PF13949	0.463
LIG_MLH1_MIPbox_1	481	485	PF16413	0.555
LIG_PCNA_yPIPBox_3	266	280	PF02747	0.486
LIG_PDZ_Class_2	700	705	PF00595	0.633
LIG_Pex14_2	347	351	PF04695	0.500
LIG_Pex14_2	474	478	PF04695	0.504
LIG_SH2_CRK	110	114	PF00017	0.436
LIG_SH2_CRK	324	328	PF00017	0.502
LIG_SH2_CRK	504	508	PF00017	0.386
LIG_SH2_CRK	53	57	PF00017	0.189
LIG_SH2_CRK	552	556	PF00017	0.429
LIG_SH2_NCK_1	317	321	PF00017	0.526
LIG_SH2_PTP2	532	535	PF00017	0.423
LIG_SH2_SRC	288	291	PF00017	0.519
LIG_SH2_SRC	626	629	PF00017	0.415
LIG_SH2_STAP1	317	321	PF00017	0.518
LIG_SH2_STAP1	502	506	PF00017	0.364
LIG_SH2_STAP1	552	556	PF00017	0.340
LIG_SH2_STAT3	331	334	PF00017	0.571
LIG_SH2_STAT3	586	589	PF00017	0.538
LIG_SH2_STAT5	165	168	PF00017	0.521
LIG_SH2_STAT5	234	237	PF00017	0.527
LIG_SH2_STAT5	288	291	PF00017	0.515
LIG_SH2_STAT5	376	379	PF00017	0.524
LIG_SH2_STAT5	45	48	PF00017	0.385
LIG_SH2_STAT5	532	535	PF00017	0.382
LIG_SH2_STAT5	586	589	PF00017	0.491
LIG_SH2_STAT5	606	609	PF00017	0.378
LIG_SH2_STAT5	626	629	PF00017	0.385
LIG_SH3_3	576	582	PF00018	0.502
LIG_SH3_4	12	19	PF00018	0.550
LIG_SUMO_SIM_anti_2	516	524	PF11976	0.348
LIG_SUMO_SIM_par_1	281	287	PF11976	0.487
LIG_SUMO_SIM_par_1	516	524	PF11976	0.423
LIG_SUMO_SIM_par_1	555	561	PF11976	0.401
LIG_SUMO_SIM_par_1	626	632	PF11976	0.470
LIG_TYR_ITIM	108	113	PF00017	0.217
LIG_UBA3_1	215	222	PF00899	0.405
LIG_UBA3_1	35	40	PF00899	0.381
LIG_UBA3_1	377	385	PF00899	0.575
LIG_UBA3_1	519	525	PF00899	0.519
LIG_WRC_WIRS_1	507	512	PF05994	0.269
LIG_WRC_WIRS_1	658	663	PF05994	0.463
MOD_CDK_SPK_2	217	222	PF00069	0.575
MOD_CDK_SPxK_1	379	385	PF00069	0.525
MOD_CK1_1	381	387	PF00069	0.485
MOD_CK1_1	524	530	PF00069	0.502
MOD_CK1_1	668	674	PF00069	0.499
MOD_CK2_1	308	314	PF00069	0.489
MOD_CK2_1	436	442	PF00069	0.509
MOD_CK2_1	555	561	PF00069	0.415
MOD_CK2_1	67	73	PF00069	0.330
MOD_CMANNOS	475	478	PF00535	0.304
MOD_Cter_Amidation	161	164	PF01082	0.313
MOD_Cter_Amidation	692	695	PF01082	0.597
MOD_GlcNHglycan	110	113	PF01048	0.452
MOD_GlcNHglycan	131	134	PF01048	0.552
MOD_GlcNHglycan	22	25	PF01048	0.358
MOD_GlcNHglycan	276	279	PF01048	0.318
MOD_GlcNHglycan	486	489	PF01048	0.302
MOD_GlcNHglycan	61	64	PF01048	0.268
MOD_GlcNHglycan	79	82	PF01048	0.384
MOD_GSK3_1	166	173	PF00069	0.495
MOD_GSK3_1	181	188	PF00069	0.475
MOD_GSK3_1	20	27	PF00069	0.368
MOD_GSK3_1	284	291	PF00069	0.537
MOD_GSK3_1	353	360	PF00069	0.530
MOD_GSK3_1	588	595	PF00069	0.391
MOD_GSK3_1	631	638	PF00069	0.512
MOD_GSK3_1	664	671	PF00069	0.417
MOD_GSK3_1	77	84	PF00069	0.387
MOD_N-GLC_1	217	222	PF02516	0.205
MOD_N-GLC_1	274	279	PF02516	0.314
MOD_N-GLC_1	47	52	PF02516	0.351
MOD_N-GLC_1	564	569	PF02516	0.511
MOD_N-GLC_1	573	578	PF02516	0.456
MOD_N-GLC_1	77	82	PF02516	0.368
MOD_N-GLC_2	3	5	PF02516	0.459
MOD_N-GLC_2	398	400	PF02516	0.257
MOD_NEK2_1	274	279	PF00069	0.516
MOD_NEK2_1	308	313	PF00069	0.505
MOD_NEK2_1	350	355	PF00069	0.558
MOD_NEK2_1	364	369	PF00069	0.511
MOD_NEK2_1	484	489	PF00069	0.497
MOD_NEK2_1	588	593	PF00069	0.445
MOD_NEK2_1	613	618	PF00069	0.526
MOD_NEK2_2	531	536	PF00069	0.475
MOD_NEK2_2	85	90	PF00069	0.341
MOD_NMyristoyl	1	7	PF02799	0.550
MOD_PIKK_1	171	177	PF00454	0.566
MOD_PIKK_1	222	228	PF00454	0.543
MOD_PIKK_1	259	265	PF00454	0.460
MOD_PIKK_1	357	363	PF00454	0.571
MOD_PIKK_1	47	53	PF00454	0.404
MOD_PIKK_1	524	530	PF00454	0.456
MOD_PIKK_1	564	570	PF00454	0.574
MOD_PIKK_1	629	635	PF00454	0.544
MOD_Plk_1	166	172	PF00069	0.505
MOD_Plk_4	173	179	PF00069	0.513
MOD_Plk_4	284	290	PF00069	0.572
MOD_Plk_4	40	46	PF00069	0.373
MOD_Plk_4	413	419	PF00069	0.517
MOD_Plk_4	592	598	PF00069	0.447
MOD_Plk_4	668	674	PF00069	0.528
MOD_Plk_4	85	91	PF00069	0.380
MOD_ProDKin_1	217	223	PF00069	0.489
MOD_ProDKin_1	24	30	PF00069	0.389
MOD_ProDKin_1	379	385	PF00069	0.519
MOD_ProDKin_1	448	454	PF00069	0.527
MOD_SUMO_for_1	11	14	PF00179	0.502
MOD_SUMO_rev_2	10	17	PF00179	0.539
MOD_SUMO_rev_2	367	377	PF00179	0.573
MOD_SUMO_rev_2	416	423	PF00179	0.552
MOD_SUMO_rev_2	547	553	PF00179	0.455
MOD_SUMO_rev_2	88	97	PF00179	0.382
TRG_DiLeu_BaEn_1	373	378	PF01217	0.587
TRG_DiLeu_BaLyEn_6	150	155	PF01217	0.313
TRG_ENDOCYTIC_2	110	113	PF00928	0.350
TRG_ENDOCYTIC_2	324	327	PF00928	0.500
TRG_ENDOCYTIC_2	415	418	PF00928	0.527
TRG_ENDOCYTIC_2	457	460	PF00928	0.468
TRG_ENDOCYTIC_2	504	507	PF00928	0.363
TRG_ENDOCYTIC_2	53	56	PF00928	0.425
TRG_ENDOCYTIC_2	532	535	PF00928	0.401
TRG_ENDOCYTIC_2	552	555	PF00928	0.392
TRG_ENDOCYTIC_2	597	600	PF00928	0.431
TRG_ENDOCYTIC_2	606	609	PF00928	0.356
TRG_ER_diArg_1	198	200	PF00400	0.537
TRG_ER_diArg_1	492	494	PF00400	0.414
TRG_ER_diArg_1	610	612	PF00400	0.493
TRG_NLS_Bipartite_1	611	627	PF00514	0.552
TRG_NLS_MonoExtC_3	159	164	PF00514	0.310
TRG_NLS_MonoExtN_4	159	164	PF00514	0.594
TRG_NLS_MonoExtN_4	622	627	PF00514	0.530
TRG_Pf-PMV_PEXEL_1	139	143	PF00026	0.589

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8T6	Leptomonas seymouri	36%	100%
A0A0N1I8N2	Leptomonas seymouri	26%	72%
A0A0N1IGQ2	Leptomonas seymouri	69%	100%
A0A0N1ILF1	Leptomonas seymouri	34%	100%
A0A0N1IMH1	Leptomonas seymouri	32%	90%
A0A0N1P9P1	Leptomonas seymouri	32%	83%
A0A0N1PCA9	Leptomonas seymouri	28%	96%
A0A0N1PE91	Leptomonas seymouri	23%	79%
A0A0N1PFI4	Leptomonas seymouri	32%	91%
A0A0S4JLK6	Bodo saltans	34%	87%
A0A0S4JS70	Bodo saltans	24%	92%
A0A0S4KGT2	Bodo saltans	38%	93%
A0A1X0NJ61	Trypanosomatidae	45%	100%
A0A1X0NJK2	Trypanosomatidae	32%	93%
A0A1X0NJX8	Trypanosomatidae	34%	91%
A0A1X0NKT7	Trypanosomatidae	28%	89%
A0A1X0NKX8	Trypanosomatidae	31%	86%
A0A1X0NMT3	Trypanosomatidae	32%	83%
A0A1X0NW84	Trypanosomatidae	34%	100%
A0A1X0NW85	Trypanosomatidae	31%	100%
A0A1X0NW89	Trypanosomatidae	34%	82%
A0A1X0NWA6	Trypanosomatidae	28%	81%
A0A1X0NWW1	Trypanosomatidae	34%	98%
A0A3Q8IBS3	Leishmania donovani	34%	77%
A0A3Q8IDD4	Leishmania donovani	92%	100%
A0A3Q8IJT4	Leishmania donovani	24%	94%
A0A3S5H5A5	Leishmania donovani	31%	82%
A0A3S5ISG2	Trypanosoma rangeli	31%	91%
A0A3S7WW18	Leishmania donovani	34%	86%
A0A3S7WW41	Leishmania donovani	28%	95%
A0A3S7WW71	Leishmania donovani	35%	100%
A0A3S7X430	Leishmania donovani	32%	90%
A0A3S7X438	Leishmania donovani	28%	75%
A0A3S7X460	Leishmania donovani	35%	91%
A0A3S7X463	Leishmania donovani	28%	75%
A0A3S7X470	Leishmania donovani	32%	97%
A0A422MYU1	Trypanosoma rangeli	33%	77%
A0A422MYX0	Trypanosoma rangeli	33%	97%
A4H3W4	Leishmania braziliensis	32%	100%
A4HE81	Leishmania braziliensis	82%	100%
A4HJ14	Leishmania braziliensis	33%	100%
A4HJ21	Leishmania braziliensis	24%	79%
A4HJ22	Leishmania braziliensis	29%	100%
A4HJ23	Leishmania braziliensis	32%	96%
A4HJ24	Leishmania braziliensis	36%	100%
A4HS39	Leishmania infantum	32%	82%
A4HYN0	Leishmania infantum	34%	86%
A4HYW1	Leishmania infantum	34%	70%
A4HYW2	Leishmania infantum	34%	100%
A4HYW3	Leishmania infantum	28%	95%
A4HYW4	Leishmania infantum	25%	71%
A4I1J4	Leishmania infantum	93%	100%
A4I6E4	Leishmania infantum	32%	97%
A4I6E6	Leishmania infantum	35%	91%
A4I6F0	Leishmania infantum	28%	75%
A4I6K4	Leishmania infantum	32%	90%
A4I6K5	Leishmania infantum	28%	75%
A4I6K6	Leishmania infantum	24%	94%
C9ZIE8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	32%	100%
C9ZIE9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	32%	96%
C9ZN52	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	33%	89%
C9ZN53	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	29%	83%
C9ZWY4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	28%	88%
C9ZY36	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	32%	82%
E8NHF1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	39%	100%
E8NHG6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E8NHG7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	100%
E8NHG8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
E8NHM2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
E8NHM4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
E8NHM8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	37%	100%
E9AIH1	Leishmania braziliensis	34%	100%
E9AIH3	Leishmania braziliensis	33%	100%
E9AIH4	Leishmania braziliensis	29%	100%
E9AIH6	Leishmania braziliensis	25%	99%
E9AK26	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E9AUQ7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	100%
E9AUQ9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
E9AUR0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	100%
E9AUR1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	99%
E9B1J0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	33%	90%
E9B1J1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	75%
E9B1J2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	22%	94%
E9B1J6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
Q22036	Caenorhabditis elegans	27%	100%
Q4Q6L7	Leishmania major	34%	100%
Q4Q6L9	Leishmania major	32%	100%
Q4Q6M0	Leishmania major	29%	100%
Q4Q6M2	Leishmania major	21%	100%
Q4Q6M3	Leishmania major	27%	76%
Q4Q6M4	Leishmania major	32%	100%
Q4Q9U3	Leishmania major	92%	100%
Q4QCS6	Leishmania major	29%	100%
Q4QCS7	Leishmania major	34%	100%
Q4QCS8	Leishmania major	34%	100%
Q4QCS9	Leishmania major	34%	100%
Q9U0T9	Leishmania major	31%	100%
V5AYJ1	Trypanosoma cruzi	35%	99%
V5B5I4	Trypanosoma cruzi	33%	84%
V5BA05	Trypanosoma cruzi	33%	100%
V5BEL3	Trypanosoma cruzi	34%	100%
V5BN20	Trypanosoma cruzi	33%	91%
V5D5V8	Trypanosoma cruzi	29%	95%
V5D9Y2	Trypanosoma cruzi	31%	83%
V5DES7	Trypanosoma cruzi	31%	89%