Diphthine--ammonia ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

MJ0570-related uncharacterized domain containing protein, putative

Species:

Leishmania mexicana

UniProt:

E9AXA6_LEIMU

TriTrypDb:

LmxM.25.0300

Length:

615

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

E9AXA6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AXA6

Function

Biological processes
Term	Name	Level	Count
GO:0006417	regulation of translation	6	1
GO:0006448	regulation of translational elongation	7	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009058	biosynthetic process	2	1
GO:0009889	regulation of biosynthetic process	4	1
GO:0009987	cellular process	1	1
GO:0010468	regulation of gene expression	5	1
GO:0010556	regulation of macromolecule biosynthetic process	5	1
GO:0010608	post-transcriptional regulation of gene expression	6	1
GO:0017182	peptidyl-diphthamide metabolic process	7	1
GO:0017183	peptidyl-diphthamide biosynthetic process from peptidyl-histidine	4	1
GO:0018193	peptidyl-amino acid modification	5	1
GO:0018202	peptidyl-histidine modification	6	1
GO:0019222	regulation of metabolic process	3	1
GO:0019538	protein metabolic process	3	1
GO:0031323	regulation of cellular metabolic process	4	1
GO:0031326	regulation of cellular biosynthetic process	5	1
GO:0034248	regulation of amide metabolic process	5	1
GO:0036211	protein modification process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044249	cellular biosynthetic process	3	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0051171	regulation of nitrogen compound metabolic process	4	1
GO:0051246	regulation of protein metabolic process	5	1
GO:0060255	regulation of macromolecule metabolic process	4	1
GO:0065007	biological regulation	1	1
GO:0071704	organic substance metabolic process	2	1
GO:0080090	regulation of primary metabolic process	4	1
GO:1900247	regulation of cytoplasmic translational elongation	8	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:2000112	obsolete regulation of cellular macromolecule biosynthetic process	6	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	10
GO:0016874	ligase activity	2	10
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	10
GO:0016880	acid-ammonia (or amide) ligase activity	4	10
GO:0017178	diphthine-ammonia ligase activity	5	10
GO:0000166	nucleotide binding	3	4
GO:0005488	binding	1	4
GO:0005524	ATP binding	5	4
GO:0017076	purine nucleotide binding	4	4
GO:0030554	adenyl nucleotide binding	5	4
GO:0032553	ribonucleotide binding	3	4
GO:0032555	purine ribonucleotide binding	4	4
GO:0032559	adenyl ribonucleotide binding	5	4
GO:0035639	purine ribonucleoside triphosphate binding	4	4
GO:0036094	small molecule binding	2	4
GO:0043167	ion binding	2	4
GO:0043168	anion binding	3	4
GO:0097159	organic cyclic compound binding	2	4
GO:0097367	carbohydrate derivative binding	2	4
GO:1901265	nucleoside phosphate binding	3	4
GO:1901363	heterocyclic compound binding	2	4

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	188	190	PF00675	0.305
CLV_NRD_NRD_1	464	466	PF00675	0.377
CLV_NRD_NRD_1	81	83	PF00675	0.251
CLV_NRD_NRD_1	86	88	PF00675	0.267
CLV_PCSK_KEX2_1	188	190	PF00082	0.213
CLV_PCSK_KEX2_1	464	466	PF00082	0.377
CLV_PCSK_KEX2_1	81	83	PF00082	0.246
CLV_PCSK_KEX2_1	86	88	PF00082	0.342
CLV_PCSK_PC7_1	82	88	PF00082	0.363
CLV_PCSK_SKI1_1	327	331	PF00082	0.447
CLV_PCSK_SKI1_1	376	380	PF00082	0.376
CLV_PCSK_SKI1_1	536	540	PF00082	0.310
DEG_APCC_DBOX_1	146	154	PF00400	0.270
DEG_APCC_DBOX_1	198	206	PF00400	0.318
DEG_Nend_UBRbox_1	1	4	PF02207	0.292
DOC_CYCLIN_RxL_1	533	544	PF00134	0.406
DOC_CYCLIN_yCln2_LP_2	225	231	PF00134	0.371
DOC_CYCLIN_yCln2_LP_2	320	326	PF00134	0.413
DOC_MAPK_gen_1	279	287	PF00069	0.431
DOC_PP1_RVXF_1	138	144	PF00149	0.303
DOC_PP2B_LxvP_1	320	323	PF13499	0.437
DOC_PP2B_LxvP_1	432	435	PF13499	0.333
DOC_PP2B_LxvP_1	76	79	PF13499	0.363
DOC_PP4_FxxP_1	385	388	PF00568	0.370
DOC_USP7_MATH_1	307	311	PF00917	0.585
DOC_USP7_MATH_1	354	358	PF00917	0.479
DOC_USP7_MATH_1	388	392	PF00917	0.426
DOC_USP7_MATH_1	402	406	PF00917	0.408
DOC_USP7_MATH_1	46	50	PF00917	0.541
DOC_WW_Pin1_4	248	253	PF00397	0.369
DOC_WW_Pin1_4	376	381	PF00397	0.431
DOC_WW_Pin1_4	418	423	PF00397	0.333
DOC_WW_Pin1_4	477	482	PF00397	0.416
LIG_14-3-3_CanoR_1	262	268	PF00244	0.375
LIG_14-3-3_CanoR_1	474	484	PF00244	0.465
LIG_14-3-3_CanoR_1	536	541	PF00244	0.404
LIG_14-3-3_CanoR_1	550	554	PF00244	0.404
LIG_14-3-3_CanoR_1	599	603	PF00244	0.388
LIG_Actin_WH2_2	65	83	PF00022	0.270
LIG_BIR_III_2	290	294	PF00653	0.276
LIG_BRCT_BRCA1_1	327	331	PF00533	0.582
LIG_BRCT_BRCA1_1	472	476	PF00533	0.308
LIG_FHA_1	1	7	PF00498	0.300
LIG_FHA_1	178	184	PF00498	0.242
LIG_FHA_1	220	226	PF00498	0.246
LIG_FHA_1	434	440	PF00498	0.344
LIG_FHA_1	485	491	PF00498	0.327
LIG_FHA_1	519	525	PF00498	0.286
LIG_FHA_1	573	579	PF00498	0.324
LIG_FHA_1	593	599	PF00498	0.408
LIG_FHA_2	114	120	PF00498	0.363
LIG_FHA_2	192	198	PF00498	0.363
LIG_FHA_2	236	242	PF00498	0.245
LIG_FHA_2	490	496	PF00498	0.382
LIG_LIR_Apic_2	347	351	PF02991	0.413
LIG_LIR_Gen_1	108	118	PF02991	0.281
LIG_LIR_Gen_1	152	161	PF02991	0.363
LIG_LIR_Gen_1	470	479	PF02991	0.310
LIG_LIR_Gen_1	535	545	PF02991	0.358
LIG_LIR_Gen_1	58	69	PF02991	0.246
LIG_LIR_Nem_3	108	114	PF02991	0.252
LIG_LIR_Nem_3	119	124	PF02991	0.330
LIG_LIR_Nem_3	379	385	PF02991	0.334
LIG_LIR_Nem_3	470	475	PF02991	0.257
LIG_LIR_Nem_3	535	540	PF02991	0.419
LIG_LIR_Nem_3	544	548	PF02991	0.335
LIG_LIR_Nem_3	58	64	PF02991	0.246
LIG_PDZ_Class_3	610	615	PF00595	0.477
LIG_Pex14_2	381	385	PF04695	0.350
LIG_Pex14_2	472	476	PF04695	0.210
LIG_Pex14_2	541	545	PF04695	0.487
LIG_SH2_CRK	111	115	PF00017	0.270
LIG_SH2_CRK	348	352	PF00017	0.406
LIG_SH2_CRK	61	65	PF00017	0.363
LIG_SH2_STAP1	220	224	PF00017	0.363
LIG_SH2_STAP1	400	404	PF00017	0.410
LIG_SH2_STAT3	59	62	PF00017	0.246
LIG_SH2_STAT3	97	100	PF00017	0.282
LIG_SH2_STAT5	155	158	PF00017	0.363
LIG_SH2_STAT5	503	506	PF00017	0.285
LIG_SH2_STAT5	537	540	PF00017	0.358
LIG_SH3_3	299	305	PF00018	0.360
LIG_SH3_3	404	410	PF00018	0.301
LIG_SUMO_SIM_anti_2	238	244	PF11976	0.328
LIG_SUMO_SIM_anti_2	3	8	PF11976	0.307
LIG_SUMO_SIM_par_1	2	8	PF11976	0.283
LIG_SUMO_SIM_par_1	256	261	PF11976	0.401
LIG_TRAF2_1	101	104	PF00917	0.311
LIG_TRAF2_1	194	197	PF00917	0.306
LIG_TRAF2_1	358	361	PF00917	0.208
LIG_TRAF2_1	610	613	PF00917	0.578
LIG_TRFH_1	301	305	PF08558	0.365
MOD_CDK_SPxK_1	477	483	PF00069	0.363
MOD_CK1_1	109	115	PF00069	0.267
MOD_CK1_1	314	320	PF00069	0.634
MOD_CK1_1	395	401	PF00069	0.501
MOD_CK2_1	191	197	PF00069	0.348
MOD_CK2_1	235	241	PF00069	0.245
MOD_CK2_1	354	360	PF00069	0.372
MOD_CK2_1	383	389	PF00069	0.362
MOD_CK2_1	590	596	PF00069	0.444
MOD_DYRK1A_RPxSP_1	376	380	PF00069	0.343
MOD_GlcNHglycan	128	131	PF01048	0.306
MOD_GlcNHglycan	252	255	PF01048	0.343
MOD_GlcNHglycan	305	308	PF01048	0.612
MOD_GlcNHglycan	309	312	PF01048	0.677
MOD_GlcNHglycan	313	316	PF01048	0.719
MOD_GlcNHglycan	327	330	PF01048	0.403
MOD_GlcNHglycan	333	336	PF01048	0.461
MOD_GlcNHglycan	356	359	PF01048	0.447
MOD_GlcNHglycan	397	400	PF01048	0.246
MOD_GlcNHglycan	47	51	PF01048	0.427
MOD_GlcNHglycan	477	480	PF01048	0.308
MOD_GlcNHglycan	592	595	PF01048	0.402
MOD_GlcNHglycan	7	10	PF01048	0.291
MOD_GSK3_1	109	116	PF00069	0.363
MOD_GSK3_1	229	236	PF00069	0.213
MOD_GSK3_1	303	310	PF00069	0.594
MOD_GSK3_1	314	321	PF00069	0.683
MOD_GSK3_1	364	371	PF00069	0.428
MOD_GSK3_1	388	395	PF00069	0.312
MOD_GSK3_1	412	419	PF00069	0.497
MOD_GSK3_1	514	521	PF00069	0.566
MOD_GSK3_1	549	556	PF00069	0.273
MOD_GSK3_1	568	575	PF00069	0.383
MOD_GSK3_1	91	98	PF00069	0.386
MOD_NEK2_1	113	118	PF00069	0.248
MOD_NEK2_1	177	182	PF00069	0.232
MOD_NEK2_1	208	213	PF00069	0.266
MOD_NEK2_1	235	240	PF00069	0.288
MOD_NEK2_1	258	263	PF00069	0.487
MOD_NEK2_1	294	299	PF00069	0.455
MOD_NEK2_1	331	336	PF00069	0.542
MOD_NEK2_1	412	417	PF00069	0.438
MOD_NEK2_1	475	480	PF00069	0.420
MOD_NEK2_1	541	546	PF00069	0.469
MOD_NEK2_1	60	65	PF00069	0.363
MOD_NEK2_2	392	397	PF00069	0.292
MOD_PIKK_1	458	464	PF00454	0.502
MOD_PIKK_1	507	513	PF00454	0.477
MOD_PIKK_1	569	575	PF00454	0.376
MOD_PK_1	179	185	PF00069	0.270
MOD_PKA_2	198	204	PF00069	0.363
MOD_PKA_2	311	317	PF00069	0.550
MOD_PKA_2	416	422	PF00069	0.512
MOD_PKA_2	549	555	PF00069	0.378
MOD_PKA_2	598	604	PF00069	0.508
MOD_Plk_1	220	226	PF00069	0.335
MOD_Plk_1	318	324	PF00069	0.396
MOD_Plk_1	374	380	PF00069	0.349
MOD_Plk_1	91	97	PF00069	0.363
MOD_Plk_4	10	16	PF00069	0.308
MOD_Plk_4	106	112	PF00069	0.230
MOD_Plk_4	113	119	PF00069	0.306
MOD_Plk_4	220	226	PF00069	0.249
MOD_Plk_4	364	370	PF00069	0.418
MOD_Plk_4	536	542	PF00069	0.308
MOD_Plk_4	549	555	PF00069	0.342
MOD_Plk_4	55	61	PF00069	0.363
MOD_Plk_4	91	97	PF00069	0.371
MOD_ProDKin_1	248	254	PF00069	0.362
MOD_ProDKin_1	376	382	PF00069	0.426
MOD_ProDKin_1	418	424	PF00069	0.336
MOD_ProDKin_1	477	483	PF00069	0.416
TRG_DiLeu_BaEn_1	272	277	PF01217	0.236
TRG_DiLeu_BaEn_4	272	278	PF01217	0.239
TRG_DiLeu_BaLyEn_6	186	191	PF01217	0.147
TRG_DiLeu_BaLyEn_6	290	295	PF01217	0.422
TRG_ENDOCYTIC_2	111	114	PF00928	0.270
TRG_ENDOCYTIC_2	155	158	PF00928	0.363
TRG_ENDOCYTIC_2	382	385	PF00928	0.455
TRG_ENDOCYTIC_2	537	540	PF00928	0.358
TRG_ENDOCYTIC_2	61	64	PF00928	0.399
TRG_ER_diArg_1	138	141	PF00400	0.270
TRG_ER_diArg_1	187	189	PF00400	0.213
TRG_ER_diArg_1	464	466	PF00400	0.399
TRG_ER_diArg_1	80	82	PF00400	0.246
TRG_ER_diArg_1	85	87	PF00400	0.246
TRG_NES_CRM1_1	162	174	PF08389	0.327
TRG_NES_CRM1_1	596	609	PF08389	0.368
TRG_Pf-PMV_PEXEL_1	193	197	PF00026	0.363
TRG_Pf-PMV_PEXEL_1	87	92	PF00026	0.267

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PEV4	Leptomonas seymouri	59%	100%
A0A1X0P4I2	Trypanosomatidae	39%	100%
A0A3R7K7F7	Trypanosoma rangeli	40%	100%
A0A3S7WYW4	Leishmania donovani	89%	100%
A4HDX7	Leishmania braziliensis	79%	100%
A4I172	Leishmania infantum	89%	100%
D0A5F8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	38%	100%
Q4QA66	Leishmania major	89%	100%
Q9USQ7	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	30%	100%