SpoU_methylase domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

SpoU rRNA Methylase family, putative

Species:

Leishmania mexicana

UniProt:

E9AX80_LEIMU

TriTrypDb:

LmxM.24.2340

Length:

479

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0005829	cytosol	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AX80

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AX80

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006396	RNA processing	6	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0001510	RNA methylation	4	1
GO:0002128	tRNA nucleoside ribose methylation	6	1
GO:0006399	tRNA metabolic process	7	1
GO:0006400	tRNA modification	6	1
GO:0008033	tRNA processing	8	1
GO:0009451	RNA modification	5	1
GO:0030488	tRNA methylation	5	1
GO:0032259	methylation	2	1
GO:0034470	ncRNA processing	7	1
GO:0034660	ncRNA metabolic process	6	1
GO:0043412	macromolecule modification	4	1
GO:0043414	macromolecule methylation	3	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	10
GO:0003723	RNA binding	4	10
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	10
GO:0008168	methyltransferase activity	4	12
GO:0008173	RNA methyltransferase activity	4	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0097159	organic cyclic compound binding	2	10
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901363	heterocyclic compound binding	2	10
GO:0008171	O-methyltransferase activity	5	1
GO:0008175	tRNA methyltransferase activity	5	1
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	1
GO:0016300	tRNA (uracil) methyltransferase activity	6	1
GO:0016427	tRNA (cytosine) methyltransferase activity	6	1
GO:0052665	tRNA (uracil-2'-O-)-methyltransferase activity	7	1
GO:0052666	tRNA (cytosine-2'-O-)-methyltransferase activity	7	1
GO:0062105	RNA 2'-O-methyltransferase activity	5	1
GO:0106050	tRNA 2'-O-methyltransferase activity	6	1
GO:0140101	catalytic activity, acting on a tRNA	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	148	152	PF00656	0.530
CLV_NRD_NRD_1	242	244	PF00675	0.422
CLV_NRD_NRD_1	283	285	PF00675	0.428
CLV_NRD_NRD_1	287	289	PF00675	0.458
CLV_NRD_NRD_1	400	402	PF00675	0.539
CLV_NRD_NRD_1	7	9	PF00675	0.483
CLV_PCSK_KEX2_1	241	243	PF00082	0.396
CLV_PCSK_KEX2_1	282	284	PF00082	0.453
CLV_PCSK_KEX2_1	287	289	PF00082	0.458
CLV_PCSK_KEX2_1	399	401	PF00082	0.524
CLV_PCSK_KEX2_1	7	9	PF00082	0.594
CLV_PCSK_PC1ET2_1	399	401	PF00082	0.524
CLV_PCSK_PC7_1	283	289	PF00082	0.504
CLV_PCSK_SKI1_1	103	107	PF00082	0.275
CLV_PCSK_SKI1_1	235	239	PF00082	0.446
CLV_PCSK_SKI1_1	404	408	PF00082	0.483
CLV_PCSK_SKI1_1	85	89	PF00082	0.375
DEG_APCC_DBOX_1	399	407	PF00400	0.516
DEG_Nend_Nbox_1	1	3	PF02207	0.643
DOC_ANK_TNKS_1	286	293	PF00023	0.285
DOC_CDC14_PxL_1	323	331	PF14671	0.474
DOC_CYCLIN_RxL_1	232	240	PF00134	0.502
DOC_CYCLIN_yClb1_LxF_4	83	88	PF00134	0.575
DOC_CYCLIN_yCln2_LP_2	321	327	PF00134	0.510
DOC_MAPK_gen_1	241	249	PF00069	0.392
DOC_MAPK_gen_1	399	410	PF00069	0.474
DOC_MAPK_HePTP_8	398	410	PF00069	0.504
DOC_MAPK_MEF2A_6	130	139	PF00069	0.522
DOC_MAPK_MEF2A_6	242	251	PF00069	0.393
DOC_MAPK_MEF2A_6	401	410	PF00069	0.471
DOC_PP1_RVXF_1	33	39	PF00149	0.478
DOC_PP1_RVXF_1	83	89	PF00149	0.575
DOC_PP2B_LxvP_1	408	411	PF13499	0.365
DOC_PP4_FxxP_1	199	202	PF00568	0.404
DOC_USP7_MATH_1	177	181	PF00917	0.560
DOC_USP7_MATH_1	461	465	PF00917	0.459
DOC_WW_Pin1_4	104	109	PF00397	0.480
DOC_WW_Pin1_4	209	214	PF00397	0.730
DOC_WW_Pin1_4	27	32	PF00397	0.533
DOC_WW_Pin1_4	413	418	PF00397	0.430
DOC_WW_Pin1_4	430	435	PF00397	0.446
DOC_WW_Pin1_4	71	76	PF00397	0.493
LIG_14-3-3_CanoR_1	130	138	PF00244	0.515
LIG_14-3-3_CanoR_1	215	221	PF00244	0.599
LIG_14-3-3_CanoR_1	241	246	PF00244	0.416
LIG_14-3-3_CanoR_1	389	398	PF00244	0.514
LIG_Actin_RPEL_3	234	253	PF02755	0.424
LIG_Actin_WH2_2	388	406	PF00022	0.484
LIG_Actin_WH2_2	446	461	PF00022	0.419
LIG_APCC_ABBA_1	251	256	PF00400	0.404
LIG_BIR_III_4	15	19	PF00653	0.600
LIG_BRCT_BRCA1_1	195	199	PF00533	0.639
LIG_BRCT_BRCA1_1	343	347	PF00533	0.625
LIG_BRCT_BRCA1_1	432	436	PF00533	0.486
LIG_deltaCOP1_diTrp_1	118	127	PF00928	0.575
LIG_deltaCOP1_diTrp_1	15	23	PF00928	0.452
LIG_EH1_1	113	121	PF00400	0.450
LIG_eIF4E_1	47	53	PF01652	0.477
LIG_FHA_1	242	248	PF00498	0.353
LIG_FHA_1	291	297	PF00498	0.409
LIG_FHA_1	31	37	PF00498	0.502
LIG_FHA_1	380	386	PF00498	0.384
LIG_FHA_2	302	308	PF00498	0.469
LIG_HP1_1	370	374	PF01393	0.258
LIG_LIR_Apic_2	196	202	PF02991	0.583
LIG_LIR_Gen_1	275	280	PF02991	0.468
LIG_LIR_LC3C_4	368	373	PF02991	0.539
LIG_LIR_Nem_3	180	185	PF02991	0.237
LIG_LIR_Nem_3	275	279	PF02991	0.507
LIG_LIR_Nem_3	318	323	PF02991	0.397
LIG_LYPXL_S_1	374	378	PF13949	0.248
LIG_LYPXL_yS_3	326	329	PF13949	0.420
LIG_LYPXL_yS_3	375	378	PF13949	0.521
LIG_PCNA_yPIPBox_3	231	243	PF02747	0.515
LIG_PTB_Apo_2	270	277	PF02174	0.461
LIG_PTB_Apo_2	295	302	PF02174	0.464
LIG_PTB_Phospho_1	270	276	PF10480	0.462
LIG_REV1ctd_RIR_1	56	63	PF16727	0.549
LIG_SH2_CRK	162	166	PF00017	0.470
LIG_SH2_PTP2	276	279	PF00017	0.486
LIG_SH2_STAP1	245	249	PF00017	0.520
LIG_SH2_STAP1	317	321	PF00017	0.410
LIG_SH2_STAT3	158	161	PF00017	0.470
LIG_SH2_STAT3	19	22	PF00017	0.577
LIG_SH2_STAT3	245	248	PF00017	0.407
LIG_SH2_STAT5	158	161	PF00017	0.479
LIG_SH2_STAT5	276	279	PF00017	0.472
LIG_SH2_STAT5	430	433	PF00017	0.404
LIG_SH3_3	105	111	PF00018	0.480
LIG_SH3_3	321	327	PF00018	0.502
LIG_SH3_3	370	376	PF00018	0.525
LIG_SH3_3	428	434	PF00018	0.401
LIG_SH3_3	468	474	PF00018	0.595
LIG_SH3_3	58	64	PF00018	0.529
LIG_SUMO_SIM_anti_2	275	281	PF11976	0.442
LIG_SUMO_SIM_par_1	406	413	PF11976	0.383
LIG_TRAF2_1	115	118	PF00917	0.578
LIG_TYR_ITIM	252	257	PF00017	0.510
LIG_TYR_ITIM	373	378	PF00017	0.539
LIG_UBA3_1	28	35	PF00899	0.542
LIG_UBA3_1	86	94	PF00899	0.529
MOD_CDC14_SPxK_1	212	215	PF00782	0.746
MOD_CDK_SPxK_1	209	215	PF00069	0.727
MOD_CK1_1	164	170	PF00069	0.479
MOD_CK1_1	30	36	PF00069	0.506
MOD_CK1_1	341	347	PF00069	0.620
MOD_CK1_1	390	396	PF00069	0.426
MOD_CK1_1	68	74	PF00069	0.560
MOD_GlcNHglycan	131	134	PF01048	0.353
MOD_GlcNHglycan	171	174	PF01048	0.280
MOD_GlcNHglycan	81	84	PF01048	0.375
MOD_GSK3_1	188	195	PF00069	0.631
MOD_GSK3_1	338	345	PF00069	0.641
MOD_GSK3_1	461	468	PF00069	0.565
MOD_GSK3_1	65	72	PF00069	0.469
MOD_LATS_1	63	69	PF00433	0.562
MOD_N-GLC_1	342	347	PF02516	0.649
MOD_N-GLC_1	68	73	PF02516	0.234
MOD_N-GLC_1	97	102	PF02516	0.321
MOD_N-GLC_2	154	156	PF02516	0.250
MOD_NEK2_1	193	198	PF00069	0.548
MOD_NEK2_1	227	232	PF00069	0.544
MOD_NEK2_1	247	252	PF00069	0.350
MOD_NEK2_1	301	306	PF00069	0.417
MOD_NEK2_1	465	470	PF00069	0.353
MOD_NEK2_1	70	75	PF00069	0.516
MOD_NEK2_1	88	93	PF00069	0.504
MOD_NEK2_1	97	102	PF00069	0.411
MOD_NEK2_2	379	384	PF00069	0.252
MOD_PIKK_1	143	149	PF00454	0.588
MOD_PIKK_1	358	364	PF00454	0.553
MOD_PIKK_1	438	444	PF00454	0.518
MOD_PKA_1	241	247	PF00069	0.424
MOD_PKA_2	129	135	PF00069	0.469
MOD_PKA_2	216	222	PF00069	0.646
MOD_PKA_2	241	247	PF00069	0.405
MOD_Plk_1	342	348	PF00069	0.464
MOD_Plk_1	387	393	PF00069	0.462
MOD_Plk_4	154	160	PF00069	0.510
MOD_Plk_4	166	172	PF00069	0.442
MOD_Plk_4	247	253	PF00069	0.350
MOD_Plk_4	343	349	PF00069	0.515
MOD_Plk_4	379	385	PF00069	0.326
MOD_Plk_4	432	438	PF00069	0.496
MOD_ProDKin_1	104	110	PF00069	0.480
MOD_ProDKin_1	209	215	PF00069	0.733
MOD_ProDKin_1	27	33	PF00069	0.528
MOD_ProDKin_1	413	419	PF00069	0.429
MOD_ProDKin_1	430	436	PF00069	0.456
MOD_ProDKin_1	71	77	PF00069	0.493
MOD_SUMO_rev_2	37	46	PF00179	0.329
TRG_ENDOCYTIC_2	162	165	PF00928	0.473
TRG_ENDOCYTIC_2	254	257	PF00928	0.420
TRG_ENDOCYTIC_2	276	279	PF00928	0.486
TRG_ENDOCYTIC_2	326	329	PF00928	0.404
TRG_ENDOCYTIC_2	375	378	PF00928	0.521
TRG_ER_diArg_1	241	243	PF00400	0.411
TRG_ER_diArg_1	282	284	PF00400	0.563
TRG_ER_diArg_1	311	314	PF00400	0.254
TRG_ER_diArg_1	6	8	PF00400	0.489
TRG_NLS_MonoCore_2	398	403	PF00514	0.524
TRG_NLS_MonoExtN_4	399	405	PF00514	0.526
TRG_Pf-PMV_PEXEL_1	235	239	PF00026	0.528
TRG_Pf-PMV_PEXEL_1	7	11	PF00026	0.614

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HUK1	Leptomonas seymouri	65%	100%
A0A0S4IV97	Bodo saltans	44%	95%
A0A1X0NN27	Trypanosomatidae	50%	100%
A0A3S7WYP3	Leishmania donovani	88%	100%
A0A422NV05	Trypanosoma rangeli	50%	100%
A4I142	Leishmania infantum	90%	100%
C9ZI50	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	48%	100%
D0AA55	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	22%	89%
E9AIR3	Leishmania braziliensis	82%	100%
Q4QA96	Leishmania major	89%	100%
V5AZL8	Trypanosoma cruzi	51%	100%