Zinc finger, C3HC4 type (RING finger)/EF-hand domain pair

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Zinc finger, C3HC4 type (RING finger)/EF-hand domain pair, putative

Species:

Leishmania mexicana

UniProt:

E9AWY1_LEIMU

TriTrypDb:

LmxM.24.1380

Length:

395

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 24
NetGPI	no	yes: 0, no: 24

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0031974	membrane-enclosed lumen	2	2
GO:0031981	nuclear lumen	5	2
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	2
GO:0043233	organelle lumen	3	2
GO:0070013	intracellular organelle lumen	4	2
GO:0097014	ciliary plasm	5	2
GO:0099568	cytoplasmic region	3	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9AWY1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AWY1

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0016567	protein ubiquitination	7	2
GO:0019538	protein metabolic process	3	2
GO:0032446	protein modification by small protein conjugation	6	2
GO:0036211	protein modification process	4	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0044238	primary metabolic process	2	2
GO:0070647	protein modification by small protein conjugation or removal	5	2
GO:0071704	organic substance metabolic process	2	2
GO:1901564	organonitrogen compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	24
GO:0005509	calcium ion binding	5	18
GO:0043167	ion binding	2	24
GO:0043169	cation binding	3	24
GO:0046872	metal ion binding	4	24
GO:0003824	catalytic activity	1	2
GO:0004842	ubiquitin-protein transferase activity	4	2
GO:0016740	transferase activity	2	2
GO:0019787	ubiquitin-like protein transferase activity	3	2
GO:0061630	ubiquitin protein ligase activity	5	2
GO:0061659	ubiquitin-like protein ligase activity	4	2
GO:0140096	catalytic activity, acting on a protein	2	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	340	344	PF00656	0.453
CLV_C14_Caspase3-7	360	364	PF00656	0.306
CLV_NRD_NRD_1	76	78	PF00675	0.592
CLV_PCSK_KEX2_1	287	289	PF00082	0.486
CLV_PCSK_KEX2_1	76	78	PF00082	0.586
CLV_PCSK_PC1ET2_1	287	289	PF00082	0.486
CLV_PCSK_SKI1_1	287	291	PF00082	0.437
DEG_SPOP_SBC_1	95	99	PF00917	0.681
DOC_CYCLIN_RxL_1	272	282	PF00134	0.343
DOC_MAPK_gen_1	287	295	PF00069	0.445
DOC_MAPK_MEF2A_6	62	71	PF00069	0.497
DOC_PP1_RVXF_1	273	280	PF00149	0.366
DOC_PP4_FxxP_1	123	126	PF00568	0.732
DOC_USP7_MATH_1	163	167	PF00917	0.780
DOC_USP7_MATH_1	182	186	PF00917	0.661
DOC_USP7_MATH_1	216	220	PF00917	0.481
DOC_USP7_MATH_1	95	99	PF00917	0.706
DOC_WW_Pin1_4	102	107	PF00397	0.604
DOC_WW_Pin1_4	122	127	PF00397	0.751
DOC_WW_Pin1_4	136	141	PF00397	0.722
DOC_WW_Pin1_4	180	185	PF00397	0.700
DOC_WW_Pin1_4	279	284	PF00397	0.421
DOC_WW_Pin1_4	96	101	PF00397	0.553
LIG_14-3-3_CanoR_1	235	240	PF00244	0.588
LIG_APCC_ABBA_1	253	258	PF00400	0.381
LIG_BIR_II_1	1	5	PF00653	0.588
LIG_CaM_IQ_9	68	83	PF13499	0.329
LIG_FHA_1	13	19	PF00498	0.476
LIG_FHA_2	280	286	PF00498	0.364
LIG_FHA_2	338	344	PF00498	0.453
LIG_LIR_Apic_2	21	26	PF02991	0.349
LIG_LIR_Gen_1	245	255	PF02991	0.338
LIG_LIR_Gen_1	326	335	PF02991	0.377
LIG_LIR_Gen_1	350	358	PF02991	0.343
LIG_LIR_Gen_1	386	392	PF02991	0.427
LIG_LIR_Nem_3	224	229	PF02991	0.555
LIG_LIR_Nem_3	245	250	PF02991	0.496
LIG_LIR_Nem_3	252	257	PF02991	0.379
LIG_LIR_Nem_3	308	312	PF02991	0.438
LIG_LIR_Nem_3	326	331	PF02991	0.430
LIG_LIR_Nem_3	350	354	PF02991	0.352
LIG_LIR_Nem_3	386	390	PF02991	0.379
LIG_LYPXL_yS_3	150	153	PF13949	0.545
LIG_SH2_CRK	247	251	PF00017	0.469
LIG_SH2_CRK	312	316	PF00017	0.566
LIG_SH2_CRK	328	332	PF00017	0.260
LIG_SH2_GRB2like	201	204	PF00017	0.527
LIG_SH2_NCK_1	312	316	PF00017	0.443
LIG_SH2_STAP1	247	251	PF00017	0.469
LIG_SH2_STAT3	201	204	PF00017	0.759
LIG_SH2_STAT3	212	215	PF00017	0.646
LIG_SH3_3	225	231	PF00018	0.627
LIG_SH3_3	97	103	PF00018	0.601
LIG_SH3_4	314	321	PF00018	0.592
LIG_SUMO_SIM_anti_2	25	31	PF11976	0.421
LIG_SUMO_SIM_par_1	379	386	PF11976	0.395
MOD_CDC14_SPxK_1	125	128	PF00782	0.478
MOD_CDK_SPK_2	96	101	PF00069	0.440
MOD_CDK_SPxK_1	122	128	PF00069	0.480
MOD_CK1_1	104	110	PF00069	0.566
MOD_CK1_1	131	137	PF00069	0.710
MOD_CK1_1	202	208	PF00069	0.776
MOD_CK1_1	221	227	PF00069	0.683
MOD_CK1_1	53	59	PF00069	0.189
MOD_CK1_1	96	102	PF00069	0.625
MOD_CK2_1	299	305	PF00069	0.381
MOD_CK2_1	380	386	PF00069	0.412
MOD_GlcNHglycan	133	136	PF01048	0.756
MOD_GlcNHglycan	165	168	PF01048	0.676
MOD_GlcNHglycan	177	180	PF01048	0.808
MOD_GlcNHglycan	184	187	PF01048	0.582
MOD_GlcNHglycan	204	207	PF01048	0.525
MOD_GlcNHglycan	261	264	PF01048	0.403
MOD_GlcNHglycan	296	300	PF01048	0.365
MOD_GSK3_1	129	136	PF00069	0.774
MOD_GSK3_1	163	170	PF00069	0.739
MOD_GSK3_1	195	202	PF00069	0.725
MOD_GSK3_1	295	302	PF00069	0.363
MOD_GSK3_1	323	330	PF00069	0.523
MOD_GSK3_1	50	57	PF00069	0.508
MOD_GSK3_1	8	15	PF00069	0.555
MOD_N-GLC_1	199	204	PF02516	0.709
MOD_N-GLC_1	259	264	PF02516	0.454
MOD_N-GLC_1	60	65	PF02516	0.501
MOD_N-GLC_2	3	5	PF02516	0.403
MOD_NEK2_1	295	300	PF00069	0.303
MOD_NEK2_1	323	328	PF00069	0.571
MOD_NEK2_2	380	385	PF00069	0.411
MOD_NEK2_2	50	55	PF00069	0.189
MOD_PIKK_1	140	146	PF00454	0.682
MOD_PIKK_1	157	163	PF00454	0.583
MOD_PIKK_1	188	194	PF00454	0.834
MOD_PIKK_1	321	327	PF00454	0.487
MOD_PKA_2	50	56	PF00069	0.367
MOD_Plk_1	129	135	PF00069	0.487
MOD_Plk_1	268	274	PF00069	0.312
MOD_Plk_1	349	355	PF00069	0.395
MOD_Plk_1	60	66	PF00069	0.501
MOD_Plk_2-3	8	14	PF00069	0.408
MOD_Plk_4	104	110	PF00069	0.534
MOD_Plk_4	249	255	PF00069	0.347
MOD_Plk_4	305	311	PF00069	0.357
MOD_Plk_4	327	333	PF00069	0.343
MOD_ProDKin_1	122	128	PF00069	0.752
MOD_ProDKin_1	136	142	PF00069	0.722
MOD_ProDKin_1	180	186	PF00069	0.702
MOD_ProDKin_1	279	285	PF00069	0.421
MOD_ProDKin_1	96	102	PF00069	0.554
TRG_DiLeu_BaEn_1	350	355	PF01217	0.454
TRG_ENDOCYTIC_2	150	153	PF00928	0.737
TRG_ENDOCYTIC_2	226	229	PF00928	0.512
TRG_ENDOCYTIC_2	247	250	PF00928	0.493
TRG_ENDOCYTIC_2	328	331	PF00928	0.459

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P229	Leptomonas seymouri	56%	100%
A0A0N1ILD7	Leptomonas seymouri	21%	87%
A0A0S4JBP2	Bodo saltans	25%	88%
A0A1X0NCU6	Trypanosomatidae	34%	100%
A0A1X0NTS2	Trypanosomatidae	25%	88%
A0A3Q8IMS6	Leishmania donovani	90%	100%
A0A3S7WWY0	Leishmania donovani	25%	88%
A0A3S7WYG1	Leishmania donovani	69%	100%
A4HDK3	Leishmania braziliensis	74%	100%
A4HDK4	Leishmania braziliensis	75%	100%
A4HZP7	Leishmania infantum	25%	88%
A4I0X7	Leishmania infantum	91%	100%
A4I0X8	Leishmania infantum	68%	100%
C9ZWH1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	28%	100%
E9AK36	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E9AVK3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	23%	100%
E9AWY2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	69%	100%
O97201	Leishmania major	31%	67%
Q4QAJ2	Leishmania major	67%	85%
Q4QAJ3	Leishmania major	89%	100%
Q4QBX6	Leishmania major	25%	88%
V5B9E6	Trypanosoma cruzi	25%	100%
V5BK04	Trypanosoma cruzi	35%	100%