EF-hand domain-containing protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania mexicana

UniProt:

E9AWV6_LEIMU

TriTrypDb:

LmxM.24.1140

Length:

380

Annotations

LeishMANIAdb annotations

Related to USP9X animal ubiquitin-specific proteases. Does not appear to be a TM protein.

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AWV6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AWV6

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	1
GO:0005509	calcium ion binding	5	1
GO:0043167	ion binding	2	1
GO:0043169	cation binding	3	1
GO:0046872	metal ion binding	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	133	137	PF00656	0.416
CLV_NRD_NRD_1	63	65	PF00675	0.513
CLV_PCSK_KEX2_1	63	65	PF00082	0.491
CLV_PCSK_SKI1_1	182	186	PF00082	0.493
CLV_PCSK_SKI1_1	218	222	PF00082	0.328
CLV_PCSK_SKI1_1	252	256	PF00082	0.367
CLV_PCSK_SKI1_1	269	273	PF00082	0.371
CLV_PCSK_SKI1_1	35	39	PF00082	0.571
CLV_PCSK_SKI1_1	63	67	PF00082	0.538
DEG_APCC_DBOX_1	181	189	PF00400	0.420
DEG_Nend_UBRbox_1	1	4	PF02207	0.519
DEG_SPOP_SBC_1	49	53	PF00917	0.507
DOC_MAPK_gen_1	140	149	PF00069	0.418
DOC_MAPK_MEF2A_6	142	151	PF00069	0.412
DOC_MAPK_MEF2A_6	198	207	PF00069	0.466
DOC_PP2B_LxvP_1	169	172	PF13499	0.338
DOC_PP2B_LxvP_1	232	235	PF13499	0.529
DOC_PP2B_LxvP_1	42	45	PF13499	0.470
DOC_PP4_FxxP_1	66	69	PF00568	0.432
DOC_USP7_MATH_1	126	130	PF00917	0.569
DOC_USP7_MATH_1	275	279	PF00917	0.390
DOC_USP7_MATH_1	69	73	PF00917	0.597
DOC_USP7_MATH_1	76	80	PF00917	0.619
DOC_WW_Pin1_4	154	159	PF00397	0.310
LIG_14-3-3_CanoR_1	15	19	PF00244	0.547
LIG_14-3-3_CanoR_1	305	312	PF00244	0.484
LIG_14-3-3_CanoR_1	375	380	PF00244	0.453
LIG_14-3-3_CanoR_1	48	58	PF00244	0.602
LIG_14-3-3_CanoR_1	75	81	PF00244	0.597
LIG_APCC_ABBA_1	244	249	PF00400	0.343
LIG_BRCT_BRCA1_1	277	281	PF00533	0.392
LIG_EH_1	67	71	PF12763	0.411
LIG_FHA_1	271	277	PF00498	0.489
LIG_FHA_1	294	300	PF00498	0.422
LIG_FHA_1	4	10	PF00498	0.755
LIG_FHA_1	50	56	PF00498	0.572
LIG_FHA_2	155	161	PF00498	0.322
LIG_FHA_2	93	99	PF00498	0.643
LIG_LIR_Gen_1	141	151	PF02991	0.394
LIG_LIR_Gen_1	160	169	PF02991	0.301
LIG_LIR_Gen_1	278	289	PF02991	0.393
LIG_LIR_LC3C_4	183	187	PF02991	0.424
LIG_LIR_Nem_3	141	147	PF02991	0.389
LIG_LIR_Nem_3	160	165	PF02991	0.305
LIG_LIR_Nem_3	194	200	PF02991	0.322
LIG_LIR_Nem_3	278	284	PF02991	0.384
LIG_LIR_Nem_3	28	33	PF02991	0.550
LIG_NRBOX	279	285	PF00104	0.390
LIG_NRBOX	336	342	PF00104	0.398
LIG_Pex14_2	66	70	PF04695	0.425
LIG_SH2_CRK	166	170	PF00017	0.341
LIG_SH2_CRK	334	338	PF00017	0.391
LIG_SH2_STAP1	334	338	PF00017	0.413
LIG_SH2_STAT5	166	169	PF00017	0.470
LIG_SH2_STAT5	247	250	PF00017	0.383
LIG_SH3_3	256	262	PF00018	0.249
LIG_SUMO_SIM_anti_2	145	151	PF11976	0.505
LIG_SUMO_SIM_anti_2	183	190	PF11976	0.460
LIG_SUMO_SIM_par_1	117	123	PF11976	0.245
LIG_SUMO_SIM_par_1	183	190	PF11976	0.337
LIG_SUMO_SIM_par_1	255	260	PF11976	0.245
LIG_SUMO_SIM_par_1	272	278	PF11976	0.391
LIG_SUMO_SIM_par_1	336	342	PF11976	0.419
LIG_TRAF2_1	239	242	PF00917	0.454
LIG_TYR_ITIM	164	169	PF00017	0.341
LIG_TYR_ITIM	332	337	PF00017	0.355
MOD_CK1_1	129	135	PF00069	0.523
MOD_CK1_1	17	23	PF00069	0.519
MOD_CK2_1	154	160	PF00069	0.318
MOD_CK2_1	303	309	PF00069	0.544
MOD_CK2_1	92	98	PF00069	0.544
MOD_GlcNHglycan	122	125	PF01048	0.481
MOD_GlcNHglycan	128	131	PF01048	0.505
MOD_GlcNHglycan	132	135	PF01048	0.362
MOD_GlcNHglycan	20	23	PF01048	0.698
MOD_GlcNHglycan	71	74	PF01048	0.678
MOD_GlcNHglycan	78	81	PF01048	0.634
MOD_GlcNHglycan	83	86	PF01048	0.655
MOD_GSK3_1	126	133	PF00069	0.565
MOD_GSK3_1	14	21	PF00069	0.601
MOD_GSK3_1	31	38	PF00069	0.558
MOD_GSK3_1	311	318	PF00069	0.436
MOD_GSK3_1	371	378	PF00069	0.480
MOD_GSK3_1	49	56	PF00069	0.608
MOD_NEK2_1	1	6	PF00069	0.664
MOD_NEK2_1	164	169	PF00069	0.376
MOD_NEK2_1	18	23	PF00069	0.515
MOD_NEK2_1	33	38	PF00069	0.670
MOD_NEK2_1	358	363	PF00069	0.400
MOD_NEK2_1	81	86	PF00069	0.747
MOD_PIKK_1	358	364	PF00454	0.401
MOD_PKA_2	14	20	PF00069	0.566
MOD_PKA_2	304	310	PF00069	0.437
MOD_PKA_2	49	55	PF00069	0.658
MOD_PKA_2	74	80	PF00069	0.587
MOD_PKA_2	81	87	PF00069	0.497
MOD_PKB_1	48	56	PF00069	0.440
MOD_Plk_1	269	275	PF00069	0.351
MOD_Plk_4	192	198	PF00069	0.314
MOD_Plk_4	242	248	PF00069	0.408
MOD_ProDKin_1	154	160	PF00069	0.307
MOD_SUMO_rev_2	327	333	PF00179	0.477
TRG_DiLeu_BaEn_1	107	112	PF01217	0.433
TRG_DiLeu_BaEn_1	160	165	PF01217	0.349
TRG_DiLeu_BaEn_4	241	247	PF01217	0.348
TRG_ENDOCYTIC_2	166	169	PF00928	0.341
TRG_ENDOCYTIC_2	197	200	PF00928	0.396
TRG_ENDOCYTIC_2	334	337	PF00928	0.361
TRG_ER_diArg_1	62	64	PF00400	0.606
TRG_NES_CRM1_1	141	153	PF08389	0.475
TRG_Pf-PMV_PEXEL_1	310	315	PF00026	0.406

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PBQ7	Leptomonas seymouri	63%	100%
A0A0S4IZX9	Bodo saltans	31%	83%
A0A1X0NX67	Trypanosomatidae	43%	97%
A0A3S7WYF3	Leishmania donovani	84%	92%
A0A422NQ90	Trypanosoma rangeli	51%	100%
A4HDH1	Leishmania braziliensis	69%	99%
A4I0V2	Leishmania infantum	84%	92%
D0A781	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	48%	100%
Q4QAL8	Leishmania major	83%	69%
V5BR35	Trypanosoma cruzi	51%	100%