tRNA:m(4)X modification enzyme TRM13

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

methyltransferase domain containing protein, putative

Species:

Leishmania mexicana

UniProt:

E9AWP5_LEIMU

TriTrypDb:

LmxM.24.0550

Length:

511

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

E9AWP5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AWP5

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	2
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006396	RNA processing	6	2
GO:0006399	tRNA metabolic process	7	2
GO:0006400	tRNA modification	6	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008033	tRNA processing	8	2
GO:0008152	metabolic process	1	2
GO:0009451	RNA modification	5	2
GO:0009987	cellular process	1	2
GO:0016070	RNA metabolic process	5	2
GO:0030488	tRNA methylation	5	2
GO:0032259	methylation	2	2
GO:0034470	ncRNA processing	7	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0034660	ncRNA metabolic process	6	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0043414	macromolecule methylation	3	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0046483	heterocycle metabolic process	3	2
GO:0071704	organic substance metabolic process	2	2
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	13
GO:0005488	binding	1	13
GO:0008168	methyltransferase activity	4	13
GO:0008171	O-methyltransferase activity	5	13
GO:0008173	RNA methyltransferase activity	4	13
GO:0008175	tRNA methyltransferase activity	5	13
GO:0016740	transferase activity	2	13
GO:0016741	transferase activity, transferring one-carbon groups	3	13
GO:0043167	ion binding	2	13
GO:0043169	cation binding	3	13
GO:0046872	metal ion binding	4	13
GO:0062105	RNA 2'-O-methyltransferase activity	5	13
GO:0106050	tRNA 2'-O-methyltransferase activity	6	13
GO:0140098	catalytic activity, acting on RNA	3	13
GO:0140101	catalytic activity, acting on a tRNA	4	13
GO:0140640	catalytic activity, acting on a nucleic acid	2	13

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	401	405	PF00656	0.395
CLV_C14_Caspase3-7	41	45	PF00656	0.472
CLV_C14_Caspase3-7	98	102	PF00656	0.420
CLV_NRD_NRD_1	113	115	PF00675	0.299
CLV_NRD_NRD_1	191	193	PF00675	0.454
CLV_NRD_NRD_1	214	216	PF00675	0.493
CLV_NRD_NRD_1	261	263	PF00675	0.626
CLV_NRD_NRD_1	28	30	PF00675	0.478
CLV_NRD_NRD_1	330	332	PF00675	0.303
CLV_NRD_NRD_1	484	486	PF00675	0.195
CLV_PCSK_FUR_1	259	263	PF00082	0.606
CLV_PCSK_KEX2_1	113	115	PF00082	0.299
CLV_PCSK_KEX2_1	191	193	PF00082	0.480
CLV_PCSK_KEX2_1	214	216	PF00082	0.493
CLV_PCSK_KEX2_1	261	263	PF00082	0.626
CLV_PCSK_KEX2_1	330	332	PF00082	0.304
CLV_PCSK_KEX2_1	484	486	PF00082	0.195
CLV_PCSK_PC7_1	326	332	PF00082	0.333
CLV_PCSK_SKI1_1	191	195	PF00082	0.544
CLV_PCSK_SKI1_1	326	330	PF00082	0.404
CLV_PCSK_SKI1_1	357	361	PF00082	0.358
CLV_Separin_Metazoa	251	255	PF03568	0.554
CLV_Separin_Metazoa	372	376	PF03568	0.604
DEG_Nend_Nbox_1	1	3	PF02207	0.490
DOC_CKS1_1	139	144	PF01111	0.272
DOC_PP1_RVXF_1	341	348	PF00149	0.604
DOC_PP1_RVXF_1	476	482	PF00149	0.448
DOC_PP2B_LxvP_1	177	180	PF13499	0.508
DOC_PP2B_PxIxI_1	383	389	PF00149	0.558
DOC_PP4_FxxP_1	139	142	PF00568	0.420
DOC_USP7_MATH_1	151	155	PF00917	0.386
DOC_USP7_MATH_1	209	213	PF00917	0.490
DOC_USP7_MATH_1	232	236	PF00917	0.552
DOC_USP7_MATH_1	307	311	PF00917	0.460
DOC_USP7_MATH_1	447	451	PF00917	0.536
DOC_USP7_MATH_1	472	476	PF00917	0.479
DOC_USP7_MATH_1	77	81	PF00917	0.707
DOC_WW_Pin1_4	138	143	PF00397	0.460
DOC_WW_Pin1_4	497	502	PF00397	0.488
LIG_14-3-3_CanoR_1	330	336	PF00244	0.526
LIG_14-3-3_CanoR_1	346	352	PF00244	0.551
LIG_14-3-3_CanoR_1	425	433	PF00244	0.570
LIG_14-3-3_CanoR_1	457	463	PF00244	0.479
LIG_14-3-3_CanoR_1	9	15	PF00244	0.535
LIG_Actin_WH2_2	240	256	PF00022	0.504
LIG_AP2alpha_2	303	305	PF02296	0.400
LIG_APCC_ABBA_1	339	344	PF00400	0.583
LIG_BIR_III_2	349	353	PF00653	0.604
LIG_BIR_III_4	44	48	PF00653	0.432
LIG_BIR_III_4	82	86	PF00653	0.461
LIG_BRCT_BRCA1_1	16	20	PF00533	0.428
LIG_Clathr_ClatBox_1	67	71	PF01394	0.364
LIG_FHA_1	150	156	PF00498	0.508
LIG_FHA_1	158	164	PF00498	0.525
LIG_FHA_1	226	232	PF00498	0.457
LIG_FHA_1	336	342	PF00498	0.583
LIG_FHA_1	356	362	PF00498	0.395
LIG_FHA_1	441	447	PF00498	0.429
LIG_FHA_2	399	405	PF00498	0.479
LIG_FHA_2	60	66	PF00498	0.575
LIG_FHA_2	98	104	PF00498	0.559
LIG_Integrin_RGD_1	42	44	PF01839	0.470
LIG_Integrin_RGD_1	46	48	PF01839	0.478
LIG_KLC1_Yacidic_2	400	404	PF13176	0.479
LIG_LIR_Apic_2	136	142	PF02991	0.445
LIG_LIR_Gen_1	143	151	PF02991	0.264
LIG_LIR_Gen_1	219	227	PF02991	0.405
LIG_LIR_Gen_1	418	426	PF02991	0.594
LIG_LIR_Gen_1	432	442	PF02991	0.395
LIG_LIR_Nem_3	143	149	PF02991	0.266
LIG_LIR_Nem_3	219	223	PF02991	0.426
LIG_LIR_Nem_3	225	230	PF02991	0.440
LIG_LIR_Nem_3	418	423	PF02991	0.594
LIG_LIR_Nem_3	432	437	PF02991	0.395
LIG_PCNA_TLS_4	396	403	PF02747	0.479
LIG_Pex14_1	16	20	PF04695	0.398
LIG_Pex14_2	146	150	PF04695	0.262
LIG_SH2_CRK	434	438	PF00017	0.437
LIG_SH2_SRC	402	405	PF00017	0.531
LIG_SH2_STAP1	12	16	PF00017	0.300
LIG_SH2_STAP1	159	163	PF00017	0.427
LIG_SH2_STAP1	227	231	PF00017	0.377
LIG_SH2_STAT5	159	162	PF00017	0.404
LIG_SH2_STAT5	182	185	PF00017	0.436
LIG_SH2_STAT5	227	230	PF00017	0.412
LIG_SH2_STAT5	402	405	PF00017	0.488
LIG_SH2_STAT5	458	461	PF00017	0.437
LIG_SH2_STAT5	487	490	PF00017	0.497
LIG_SH2_STAT5	494	497	PF00017	0.481
LIG_SH3_2	352	357	PF14604	0.604
LIG_SH3_3	145	151	PF00018	0.350
LIG_SH3_3	298	304	PF00018	0.374
LIG_SH3_3	349	355	PF00018	0.604
LIG_SH3_3	385	391	PF00018	0.494
LIG_SH3_3	457	463	PF00018	0.479
LIG_SUMO_SIM_par_1	222	229	PF11976	0.436
LIG_WRC_WIRS_1	227	232	PF05994	0.531
LIG_WRC_WIRS_1	78	83	PF05994	0.572
MOD_CDK_SPK_2	497	502	PF00069	0.488
MOD_CDK_SPxxK_3	497	504	PF00069	0.513
MOD_CK1_1	398	404	PF00069	0.472
MOD_CK1_1	416	422	PF00069	0.381
MOD_CK1_1	452	458	PF00069	0.497
MOD_CK1_1	54	60	PF00069	0.608
MOD_CK2_1	458	464	PF00069	0.539
MOD_CK2_1	97	103	PF00069	0.345
MOD_GlcNHglycan	126	129	PF01048	0.532
MOD_GlcNHglycan	133	136	PF01048	0.502
MOD_GlcNHglycan	196	200	PF01048	0.526
MOD_GlcNHglycan	367	370	PF01048	0.404
MOD_GlcNHglycan	408	411	PF01048	0.317
MOD_GlcNHglycan	415	418	PF01048	0.309
MOD_GlcNHglycan	87	90	PF01048	0.667
MOD_GSK3_1	10	17	PF00069	0.558
MOD_GSK3_1	318	325	PF00069	0.588
MOD_GSK3_1	331	338	PF00069	0.563
MOD_GSK3_1	47	54	PF00069	0.554
MOD_GSK3_1	59	66	PF00069	0.491
MOD_GSK3_1	87	94	PF00069	0.592
MOD_LATS_1	27	33	PF00433	0.351
MOD_N-GLC_1	429	434	PF02516	0.309
MOD_N-GLC_2	106	108	PF02516	0.275
MOD_N-GLC_2	49	51	PF02516	0.556
MOD_N-GLC_2	503	505	PF02516	0.383
MOD_NEK2_1	226	231	PF00069	0.522
MOD_NEK2_1	335	340	PF00069	0.604
MOD_NEK2_1	365	370	PF00069	0.494
MOD_NEK2_1	87	92	PF00069	0.490
MOD_NEK2_1	95	100	PF00069	0.487
MOD_NEK2_2	151	156	PF00069	0.387
MOD_NEK2_2	429	434	PF00069	0.553
MOD_PIKK_1	472	478	PF00454	0.479
MOD_PIKK_1	87	93	PF00454	0.505
MOD_PKA_1	29	35	PF00069	0.383
MOD_PKA_2	253	259	PF00069	0.566
MOD_PKA_2	345	351	PF00069	0.544
MOD_PKA_2	424	430	PF00069	0.518
MOD_PKB_1	324	332	PF00069	0.558
MOD_Plk_1	232	238	PF00069	0.471
MOD_Plk_1	429	435	PF00069	0.564
MOD_Plk_4	10	16	PF00069	0.549
MOD_Plk_4	226	232	PF00069	0.388
MOD_Plk_4	296	302	PF00069	0.376
MOD_Plk_4	398	404	PF00069	0.479
MOD_Plk_4	429	435	PF00069	0.437
MOD_Plk_4	63	69	PF00069	0.590
MOD_ProDKin_1	138	144	PF00069	0.465
MOD_ProDKin_1	497	503	PF00069	0.488
TRG_DiLeu_BaEn_1	219	224	PF01217	0.480
TRG_DiLeu_BaEn_1	63	68	PF01217	0.509
TRG_DiLeu_LyEn_5	219	224	PF01217	0.480
TRG_ENDOCYTIC_2	11	14	PF00928	0.437
TRG_ENDOCYTIC_2	227	230	PF00928	0.377
TRG_ENDOCYTIC_2	434	437	PF00928	0.437
TRG_ER_diArg_1	112	114	PF00400	0.404
TRG_ER_diArg_1	191	193	PF00400	0.513
TRG_ER_diArg_1	259	262	PF00400	0.603
TRG_ER_diArg_1	324	327	PF00400	0.496
TRG_ER_diArg_1	330	333	PF00400	0.494
TRG_Pf-PMV_PEXEL_1	105	110	PF00026	0.465
TRG_Pf-PMV_PEXEL_1	114	118	PF00026	0.489
TRG_Pf-PMV_PEXEL_1	375	380	PF00026	0.358

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A1X0NJE0	Trypanosomatidae	33%	100%
A0A3Q8IG18	Leishmania donovani	52%	100%
A0A3S7WY71	Leishmania donovani	90%	100%
A4I0P4	Leishmania infantum	52%	100%
A4I0P5	Leishmania infantum	90%	100%
D0A723	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	34%	100%
E9AIR1	Leishmania braziliensis	79%	100%
E9AWP4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	53%	94%
Q4QAS6	Leishmania major	89%	100%
Q4QAS7	Leishmania major	52%	94%
V5BR90	Trypanosoma cruzi	35%	96%