tRNA:m(4)X modification enzyme TRM13

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

methyltransferase domain containing protein, putative

Species:

Leishmania mexicana

UniProt:

E9AWP4_LEIMU

TriTrypDb:

LmxM.24.0540

Length:

462

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 13
NetGPI	no	yes: 0, no: 13

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

E9AWP4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AWP4

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	2
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006396	RNA processing	6	2
GO:0006399	tRNA metabolic process	7	2
GO:0006400	tRNA modification	6	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008033	tRNA processing	8	2
GO:0008152	metabolic process	1	2
GO:0009451	RNA modification	5	2
GO:0009987	cellular process	1	2
GO:0016070	RNA metabolic process	5	2
GO:0030488	tRNA methylation	5	2
GO:0032259	methylation	2	2
GO:0034470	ncRNA processing	7	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0034660	ncRNA metabolic process	6	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0043414	macromolecule methylation	3	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0046483	heterocycle metabolic process	3	2
GO:0071704	organic substance metabolic process	2	2
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	14
GO:0005488	binding	1	14
GO:0008168	methyltransferase activity	4	14
GO:0008171	O-methyltransferase activity	5	14
GO:0008173	RNA methyltransferase activity	4	14
GO:0008175	tRNA methyltransferase activity	5	14
GO:0016740	transferase activity	2	14
GO:0016741	transferase activity, transferring one-carbon groups	3	14
GO:0043167	ion binding	2	14
GO:0043169	cation binding	3	14
GO:0046872	metal ion binding	4	14
GO:0062105	RNA 2'-O-methyltransferase activity	5	14
GO:0106050	tRNA 2'-O-methyltransferase activity	6	14
GO:0140098	catalytic activity, acting on RNA	3	14
GO:0140101	catalytic activity, acting on a tRNA	4	14
GO:0140640	catalytic activity, acting on a nucleic acid	2	14

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	164	166	PF00675	0.609
CLV_NRD_NRD_1	233	235	PF00675	0.495
CLV_NRD_NRD_1	352	354	PF00675	0.484
CLV_NRD_NRD_1	396	398	PF00675	0.543
CLV_PCSK_FUR_1	162	166	PF00082	0.486
CLV_PCSK_KEX2_1	164	166	PF00082	0.609
CLV_PCSK_KEX2_1	233	235	PF00082	0.480
CLV_PCSK_KEX2_1	352	354	PF00082	0.484
CLV_PCSK_KEX2_1	360	362	PF00082	0.481
CLV_PCSK_KEX2_1	396	398	PF00082	0.616
CLV_PCSK_PC1ET2_1	360	362	PF00082	0.435
CLV_PCSK_PC7_1	229	235	PF00082	0.537
CLV_PCSK_SKI1_1	229	233	PF00082	0.575
CLV_PCSK_SKI1_1	260	264	PF00082	0.440
CLV_PCSK_SKI1_1	57	61	PF00082	0.386
CLV_PCSK_SKI1_1	77	81	PF00082	0.394
CLV_Separin_Metazoa	154	158	PF03568	0.429
CLV_Separin_Metazoa	275	279	PF03568	0.431
DOC_CKS1_1	445	450	PF01111	0.372
DOC_MAPK_gen_1	360	368	PF00069	0.422
DOC_MAPK_gen_1	430	438	PF00069	0.505
DOC_MAPK_gen_1	449	456	PF00069	0.311
DOC_MAPK_gen_1	83	93	PF00069	0.371
DOC_MAPK_MEF2A_6	361	370	PF00069	0.418
DOC_PP1_RVXF_1	244	251	PF00149	0.465
DOC_PP1_RVXF_1	341	348	PF00149	0.384
DOC_PP2B_PxIxI_1	286	292	PF00149	0.412
DOC_USP7_MATH_1	105	109	PF00917	0.420
DOC_USP7_MATH_1	135	139	PF00917	0.478
DOC_USP7_MATH_1	210	214	PF00917	0.443
DOC_USP7_MATH_1	351	355	PF00917	0.530
DOC_USP7_MATH_1	372	376	PF00917	0.481
DOC_USP7_MATH_1	4	8	PF00917	0.479
DOC_WW_Pin1_4	12	17	PF00397	0.531
DOC_WW_Pin1_4	444	449	PF00397	0.403
LIG_14-3-3_CanoR_1	233	239	PF00244	0.475
LIG_14-3-3_CanoR_1	249	255	PF00244	0.475
LIG_14-3-3_CanoR_1	328	334	PF00244	0.571
LIG_14-3-3_CanoR_1	57	66	PF00244	0.399
LIG_14-3-3_CterR_2	457	462	PF00244	0.415
LIG_Actin_WH2_2	143	159	PF00022	0.490
LIG_AP2alpha_2	206	208	PF02296	0.366
LIG_APCC_ABBA_1	242	247	PF00400	0.439
LIG_BIR_III_2	252	256	PF00653	0.459
LIG_FHA_1	129	135	PF00498	0.455
LIG_FHA_1	239	245	PF00498	0.488
LIG_FHA_1	259	265	PF00498	0.259
LIG_FHA_1	61	67	PF00498	0.498
LIG_FHA_2	117	123	PF00498	0.358
LIG_FHA_2	39	45	PF00498	0.552
LIG_FHA_2	8	14	PF00498	0.551
LIG_LIR_Gen_1	122	130	PF02991	0.406
LIG_LIR_Gen_1	410	421	PF02991	0.362
LIG_LIR_Nem_3	122	126	PF02991	0.425
LIG_LIR_Nem_3	128	133	PF02991	0.455
LIG_LIR_Nem_3	410	416	PF02991	0.389
LIG_LIR_Nem_3	92	96	PF02991	0.467
LIG_SH2_CRK	96	100	PF00017	0.388
LIG_SH2_GRB2like	96	99	PF00017	0.392
LIG_SH2_STAP1	130	134	PF00017	0.370
LIG_SH2_STAP1	96	100	PF00017	0.482
LIG_SH2_STAT3	310	313	PF00017	0.361
LIG_SH2_STAT5	130	133	PF00017	0.399
LIG_SH2_STAT5	305	308	PF00017	0.339
LIG_SH2_STAT5	310	313	PF00017	0.380
LIG_SH2_STAT5	441	444	PF00017	0.376
LIG_SH3_2	255	260	PF14604	0.474
LIG_SH3_3	111	117	PF00018	0.395
LIG_SH3_3	201	207	PF00018	0.377
LIG_SH3_3	252	258	PF00018	0.462
LIG_SH3_3	288	294	PF00018	0.322
LIG_SH3_3	442	448	PF00018	0.378
LIG_SUMO_SIM_par_1	125	132	PF11976	0.419
LIG_SUMO_SIM_par_1	15	20	PF11976	0.487
LIG_SUMO_SIM_par_1	29	35	PF11976	0.383
LIG_WRC_WIRS_1	130	135	PF05994	0.559
MOD_CDK_SPK_2	444	449	PF00069	0.403
MOD_CDK_SPxxK_3	444	451	PF00069	0.429
MOD_CK1_1	15	21	PF00069	0.521
MOD_CK1_1	354	360	PF00069	0.534
MOD_CK1_1	7	13	PF00069	0.508
MOD_CK2_1	116	122	PF00069	0.387
MOD_CK2_1	336	342	PF00069	0.470
MOD_CK2_1	7	13	PF00069	0.513
MOD_Cter_Amidation	358	361	PF01082	0.419
MOD_GlcNHglycan	107	110	PF01048	0.454
MOD_GlcNHglycan	270	273	PF01048	0.424
MOD_GlcNHglycan	354	357	PF01048	0.510
MOD_GlcNHglycan	378	381	PF01048	0.487
MOD_GlcNHglycan	38	41	PF01048	0.459
MOD_GlcNHglycan	397	400	PF01048	0.449
MOD_GSK3_1	105	112	PF00069	0.498
MOD_GSK3_1	221	228	PF00069	0.622
MOD_GSK3_1	234	241	PF00069	0.444
MOD_GSK3_1	332	339	PF00069	0.555
MOD_GSK3_1	351	358	PF00069	0.488
MOD_GSK3_1	36	43	PF00069	0.478
MOD_GSK3_1	372	379	PF00069	0.457
MOD_GSK3_1	395	402	PF00069	0.466
MOD_GSK3_1	403	410	PF00069	0.460
MOD_N-GLC_1	332	337	PF02516	0.525
MOD_N-GLC_1	381	386	PF02516	0.436
MOD_N-GLC_1	50	55	PF02516	0.439
MOD_N-GLC_1	67	72	PF02516	0.277
MOD_N-GLC_2	450	452	PF02516	0.429
MOD_NEK2_1	129	134	PF00069	0.532
MOD_NEK2_1	238	243	PF00069	0.469
MOD_NEK2_1	268	273	PF00069	0.379
MOD_NEK2_1	36	41	PF00069	0.485
MOD_NEK2_1	50	55	PF00069	0.598
MOD_NEK2_2	332	337	PF00069	0.591
MOD_PIKK_1	372	378	PF00454	0.487
MOD_PKA_1	352	358	PF00069	0.466
MOD_PKA_2	156	162	PF00069	0.445
MOD_PKA_2	248	254	PF00069	0.448
MOD_PKA_2	327	333	PF00069	0.475
MOD_PKA_2	351	357	PF00069	0.690
MOD_PKA_2	395	401	PF00069	0.518
MOD_PKB_1	227	235	PF00069	0.573
MOD_Plk_1	135	141	PF00069	0.483
MOD_Plk_1	332	338	PF00069	0.603
MOD_Plk_1	67	73	PF00069	0.534
MOD_Plk_4	129	135	PF00069	0.424
MOD_Plk_4	199	205	PF00069	0.379
MOD_ProDKin_1	12	18	PF00069	0.521
MOD_ProDKin_1	444	450	PF00069	0.397
MOD_SUMO_rev_2	112	119	PF00179	0.386
TRG_DiLeu_BaEn_1	122	127	PF01217	0.398
TRG_DiLeu_BaLyEn_6	45	50	PF01217	0.439
TRG_DiLeu_LyEn_5	122	127	PF01217	0.398
TRG_ENDOCYTIC_2	130	133	PF00928	0.385
TRG_ENDOCYTIC_2	423	426	PF00928	0.305
TRG_ENDOCYTIC_2	96	99	PF00928	0.462
TRG_ER_diArg_1	162	165	PF00400	0.577
TRG_ER_diArg_1	227	230	PF00400	0.491
TRG_ER_diArg_1	233	236	PF00400	0.453
TRG_ER_diArg_1	429	432	PF00400	0.268
TRG_ER_diArg_1	45	48	PF00400	0.404
TRG_Pf-PMV_PEXEL_1	278	283	PF00026	0.497

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A1X0NJE0	Trypanosomatidae	33%	95%
A0A3Q8IG18	Leishmania donovani	94%	100%
A0A3S7WY71	Leishmania donovani	52%	90%
A4HDB1	Leishmania braziliensis	79%	100%
A4I0P4	Leishmania infantum	94%	100%
A4I0P5	Leishmania infantum	52%	90%
D0A723	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	34%	91%
E9AIR1	Leishmania braziliensis	58%	100%
E9AWP5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	53%	90%
Q4QAS6	Leishmania major	52%	100%
Q4QAS7	Leishmania major	92%	100%
V5BR90	Trypanosoma cruzi	36%	87%