Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

poly A polymerase regulatory subunit, putative

Species:

Leishmania mexicana

UniProt:

E9AWK1_LEIMU

TriTrypDb:

LmxM.24.0110

Length:

438

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	3
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0110165	cellular anatomical entity	1	3

Expansion

Sequence features

E9AWK1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AWK1

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006370	7-methylguanosine mRNA capping	8	12
GO:0006396	RNA processing	6	12
GO:0006397	mRNA processing	7	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009452	7-methylguanosine RNA capping	8	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0016071	mRNA metabolic process	6	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0036260	RNA capping	7	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003746	translation elongation factor activity	4	12
GO:0003824	catalytic activity	1	12
GO:0004483	mRNA (nucleoside-2'-O-)-methyltransferase activity	6	12
GO:0005488	binding	1	12
GO:0008135	translation factor activity, RNA binding	3	12
GO:0008168	methyltransferase activity	4	12
GO:0008171	O-methyltransferase activity	5	12
GO:0008173	RNA methyltransferase activity	4	12
GO:0008174	mRNA methyltransferase activity	5	12
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0045182	translation regulator activity	1	12
GO:0090079	translation regulator activity, nucleic acid binding	2	12
GO:0097159	organic cyclic compound binding	2	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	216	220	PF00656	0.268
CLV_NRD_NRD_1	134	136	PF00675	0.534
CLV_NRD_NRD_1	15	17	PF00675	0.306
CLV_NRD_NRD_1	252	254	PF00675	0.521
CLV_NRD_NRD_1	334	336	PF00675	0.450
CLV_NRD_NRD_1	39	41	PF00675	0.230
CLV_NRD_NRD_1	411	413	PF00675	0.421
CLV_NRD_NRD_1	6	8	PF00675	0.323
CLV_PCSK_FUR_1	132	136	PF00082	0.497
CLV_PCSK_KEX2_1	134	136	PF00082	0.497
CLV_PCSK_KEX2_1	15	17	PF00082	0.305
CLV_PCSK_KEX2_1	244	246	PF00082	0.578
CLV_PCSK_KEX2_1	252	254	PF00082	0.556
CLV_PCSK_KEX2_1	411	413	PF00082	0.415
CLV_PCSK_KEX2_1	423	425	PF00082	0.455
CLV_PCSK_KEX2_1	432	434	PF00082	0.470
CLV_PCSK_KEX2_1	6	8	PF00082	0.322
CLV_PCSK_PC1ET2_1	244	246	PF00082	0.530
CLV_PCSK_PC1ET2_1	423	425	PF00082	0.468
CLV_PCSK_PC1ET2_1	432	434	PF00082	0.478
CLV_PCSK_SKI1_1	280	284	PF00082	0.467
CLV_PCSK_SKI1_1	326	330	PF00082	0.495
CLV_PCSK_SKI1_1	41	45	PF00082	0.231
CLV_PCSK_SKI1_1	6	10	PF00082	0.321
CLV_PCSK_SKI1_1	67	71	PF00082	0.227
DEG_Nend_Nbox_1	1	3	PF02207	0.528
DEG_SPOP_SBC_1	229	233	PF00917	0.230
DOC_CYCLIN_yCln2_LP_2	312	318	PF00134	0.309
DOC_CYCLIN_yCln2_LP_2	80	86	PF00134	0.409
DOC_CYCLIN_yCln2_LP_2	96	102	PF00134	0.230
DOC_MAPK_MEF2A_6	230	239	PF00069	0.230
DOC_PP2B_LxvP_1	80	83	PF13499	0.409
DOC_PP4_FxxP_1	190	193	PF00568	0.230
DOC_PP4_FxxP_1	27	30	PF00568	0.616
DOC_USP7_MATH_1	197	201	PF00917	0.243
DOC_USP7_MATH_1	229	233	PF00917	0.230
DOC_USP7_MATH_1	293	297	PF00917	0.318
DOC_WD40_RPTOR_TOS_1	179	184	PF00400	0.320
DOC_WW_Pin1_4	377	382	PF00397	0.290
DOC_WW_Pin1_4	62	67	PF00397	0.496
LIG_14-3-3_CanoR_1	132	138	PF00244	0.261
LIG_14-3-3_CanoR_1	253	259	PF00244	0.282
LIG_14-3-3_CanoR_1	326	331	PF00244	0.278
LIG_14-3-3_CanoR_1	6	14	PF00244	0.504
LIG_CaM_NSCaTE_8	327	334	PF13499	0.266
LIG_deltaCOP1_diTrp_1	176	179	PF00928	0.306
LIG_eIF4E_1	37	43	PF01652	0.438
LIG_FHA_1	113	119	PF00498	0.186
LIG_FHA_1	124	130	PF00498	0.261
LIG_FHA_1	138	144	PF00498	0.240
LIG_FHA_1	162	168	PF00498	0.230
LIG_FHA_1	219	225	PF00498	0.297
LIG_FHA_1	301	307	PF00498	0.231
LIG_FHA_2	134	140	PF00498	0.281
LIG_FHA_2	209	215	PF00498	0.376
LIG_FHA_2	255	261	PF00498	0.308
LIG_KLC1_Yacidic_2	214	219	PF13176	0.274
LIG_LIR_Apic_2	24	30	PF02991	0.621
LIG_LIR_Gen_1	213	223	PF02991	0.337
LIG_LIR_Gen_1	391	400	PF02991	0.455
LIG_LIR_Gen_1	93	102	PF02991	0.336
LIG_LIR_LC3C_4	234	239	PF02991	0.275
LIG_LIR_Nem_3	213	218	PF02991	0.337
LIG_LIR_Nem_3	309	313	PF02991	0.280
LIG_LIR_Nem_3	380	386	PF02991	0.298
LIG_LIR_Nem_3	391	395	PF02991	0.337
LIG_LIR_Nem_3	87	92	PF02991	0.446
LIG_LIR_Nem_3	93	97	PF02991	0.279
LIG_PCNA_TLS_4	280	287	PF02747	0.360
LIG_Pex14_2	383	387	PF04695	0.274
LIG_SH2_CRK	255	259	PF00017	0.405
LIG_SH2_CRK	396	400	PF00017	0.308
LIG_SH2_GRB2like	18	21	PF00017	0.622
LIG_SH2_NCK_1	247	251	PF00017	0.450
LIG_SH2_SRC	217	220	PF00017	0.303
LIG_SH2_STAP1	264	268	PF00017	0.255
LIG_SH2_STAT3	205	208	PF00017	0.243
LIG_SH2_STAT5	215	218	PF00017	0.259
LIG_SH2_STAT5	255	258	PF00017	0.299
LIG_SH2_STAT5	286	289	PF00017	0.304
LIG_SH2_STAT5	342	345	PF00017	0.409
LIG_SH2_STAT5	396	399	PF00017	0.409
LIG_SH3_1	189	195	PF00018	0.230
LIG_SH3_3	11	17	PF00018	0.637
LIG_SH3_3	189	195	PF00018	0.230
LIG_SH3_3	223	229	PF00018	0.236
LIG_SH3_3	236	242	PF00018	0.215
LIG_SH3_3	312	318	PF00018	0.294
LIG_SH3_3	98	104	PF00018	0.198
LIG_SUMO_SIM_par_1	115	122	PF11976	0.243
LIG_TRAF2_1	211	214	PF00917	0.274
LIG_TRFH_1	86	90	PF08558	0.409
LIG_TYR_ITIM	340	345	PF00017	0.324
MOD_CDC14_SPxK_1	65	68	PF00782	0.405
MOD_CDK_SPK_2	62	67	PF00069	0.496
MOD_CDK_SPxK_1	62	68	PF00069	0.496
MOD_CK1_1	23	29	PF00069	0.577
MOD_CK1_1	59	65	PF00069	0.425
MOD_CK2_1	133	139	PF00069	0.348
MOD_CK2_1	208	214	PF00069	0.309
MOD_CK2_1	228	234	PF00069	0.184
MOD_CK2_1	254	260	PF00069	0.299
MOD_CK2_1	42	48	PF00069	0.430
MOD_Cter_Amidation	429	432	PF01082	0.570
MOD_GlcNHglycan	150	153	PF01048	0.430
MOD_GlcNHglycan	287	290	PF01048	0.547
MOD_GlcNHglycan	337	340	PF01048	0.434
MOD_GlcNHglycan	61	64	PF01048	0.310
MOD_GSK3_1	104	111	PF00069	0.341
MOD_GSK3_1	133	140	PF00069	0.265
MOD_GSK3_1	144	151	PF00069	0.192
MOD_GSK3_1	19	26	PF00069	0.519
MOD_GSK3_1	298	305	PF00069	0.278
MOD_GSK3_1	326	333	PF00069	0.290
MOD_LATS_1	324	330	PF00433	0.356
MOD_N-GLC_1	19	24	PF02516	0.390
MOD_N-GLC_1	56	61	PF02516	0.187
MOD_NEK2_1	218	223	PF00069	0.334
MOD_NEK2_1	300	305	PF00069	0.315
MOD_NEK2_1	330	335	PF00069	0.309
MOD_NEK2_1	84	89	PF00069	0.427
MOD_NEK2_2	254	259	PF00069	0.288
MOD_PIKK_1	218	224	PF00454	0.353
MOD_PK_1	302	308	PF00069	0.285
MOD_PKA_1	335	341	PF00069	0.272
MOD_PKA_1	431	437	PF00069	0.709
MOD_PKA_2	133	139	PF00069	0.259
MOD_Plk_1	208	214	PF00069	0.274
MOD_Plk_2-3	213	219	PF00069	0.274
MOD_Plk_4	213	219	PF00069	0.274
MOD_Plk_4	282	288	PF00069	0.306
MOD_Plk_4	326	332	PF00069	0.307
MOD_ProDKin_1	377	383	PF00069	0.284
MOD_ProDKin_1	62	68	PF00069	0.496
TRG_DiLeu_BaEn_1	46	51	PF01217	0.444
TRG_DiLeu_BaEn_4	213	219	PF01217	0.274
TRG_DiLeu_BaLyEn_6	27	32	PF01217	0.612
TRG_DiLeu_BaLyEn_6	277	282	PF01217	0.301
TRG_ENDOCYTIC_2	215	218	PF00928	0.334
TRG_ENDOCYTIC_2	255	258	PF00928	0.380
TRG_ENDOCYTIC_2	294	297	PF00928	0.343
TRG_ENDOCYTIC_2	310	313	PF00928	0.404
TRG_ENDOCYTIC_2	342	345	PF00928	0.323
TRG_ER_diArg_1	131	134	PF00400	0.334
TRG_ER_diArg_1	14	16	PF00400	0.511
TRG_ER_diArg_1	252	254	PF00400	0.348
TRG_ER_diArg_1	55	58	PF00400	0.376
TRG_ER_diArg_1	6	8	PF00400	0.519
TRG_Pf-PMV_PEXEL_1	280	284	PF00026	0.560
TRG_Pf-PMV_PEXEL_1	7	11	PF00026	0.276

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HV56	Leptomonas seymouri	69%	100%
A0A0S4J219	Bodo saltans	48%	100%
A0A1X0NIX1	Trypanosomatidae	49%	100%
A0A3S7WY48	Leishmania donovani	89%	100%
A0A422P442	Trypanosoma rangeli	53%	100%
A4HD69	Leishmania braziliensis	83%	100%
D0A6Y2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	52%	100%
E9AH23	Leishmania infantum	89%	100%
Q4QAW9	Leishmania major	91%	100%
V5BRC4	Trypanosoma cruzi	51%	100%