Putative clathrin coat assembly protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

clathrin coat assembly protein, putative

Species:

Leishmania mexicana

UniProt:

E9AWJ2_LEIMU

TriTrypDb:

LmxM.24.0020

Length:

483

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0030135	coated vesicle	7	12
GO:0030136	clathrin-coated vesicle	8	12
GO:0031410	cytoplasmic vesicle	6	12
GO:0031982	vesicle	4	12
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0043229	intracellular organelle	3	12
GO:0043231	intracellular membrane-bounded organelle	4	12
GO:0097708	intracellular vesicle	5	12
GO:0110165	cellular anatomical entity	1	12
GO:0000331	contractile vacuole	6	1
GO:0005737	cytoplasm	2	1
GO:0005773	vacuole	5	1
GO:0005905	clathrin-coated pit	4	1
GO:0016020	membrane	2	1
GO:0098590	plasma membrane region	3	1

Expansion

Sequence features

E9AWJ2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AWJ2

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	12
GO:0006897	endocytosis	5	12
GO:0006898	receptor-mediated endocytosis	6	12
GO:0009987	cellular process	1	12
GO:0016043	cellular component organization	3	12
GO:0016192	vesicle-mediated transport	4	12
GO:0022607	cellular component assembly	4	12
GO:0043933	protein-containing complex organization	4	12
GO:0048268	clathrin coat assembly	6	12
GO:0051179	localization	1	12
GO:0051234	establishment of localization	2	12
GO:0065003	protein-containing complex assembly	5	12
GO:0071840	cellular component organization or biogenesis	2	12
GO:0072583	clathrin-dependent endocytosis	7	12
GO:0006900	vesicle budding from membrane	5	1
GO:0006996	organelle organization	4	1
GO:0016050	vesicle organization	5	1
GO:0061024	membrane organization	4	1

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	12
GO:0005515	protein binding	2	12
GO:0005543	phospholipid binding	3	12
GO:0005545	1-phosphatidylinositol binding	5	12
GO:0008289	lipid binding	2	12
GO:0030276	clathrin binding	3	12
GO:0035091	phosphatidylinositol binding	4	12
GO:0000149	SNARE binding	3	1
GO:0005546	phosphatidylinositol-4,5-bisphosphate binding	4	1
GO:0032050	clathrin heavy chain binding	4	1
GO:0043167	ion binding	2	1
GO:0043168	anion binding	3	1
GO:1901981	phosphatidylinositol phosphate binding	5	1
GO:1902936	phosphatidylinositol bisphosphate binding	6	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	103	107	PF00656	0.403
CLV_C14_Caspase3-7	129	133	PF00656	0.345
CLV_NRD_NRD_1	161	163	PF00675	0.354
CLV_NRD_NRD_1	247	249	PF00675	0.354
CLV_NRD_NRD_1	254	256	PF00675	0.324
CLV_NRD_NRD_1	318	320	PF00675	0.401
CLV_PCSK_KEX2_1	161	163	PF00082	0.339
CLV_PCSK_KEX2_1	229	231	PF00082	0.371
CLV_PCSK_KEX2_1	246	248	PF00082	0.245
CLV_PCSK_KEX2_1	254	256	PF00082	0.320
CLV_PCSK_KEX2_1	318	320	PF00082	0.474
CLV_PCSK_KEX2_1	80	82	PF00082	0.245
CLV_PCSK_PC1ET2_1	229	231	PF00082	0.371
CLV_PCSK_PC1ET2_1	246	248	PF00082	0.390
CLV_PCSK_PC1ET2_1	80	82	PF00082	0.245
CLV_PCSK_PC7_1	76	82	PF00082	0.390
CLV_PCSK_SKI1_1	116	120	PF00082	0.313
CLV_PCSK_SKI1_1	221	225	PF00082	0.298
CLV_PCSK_SKI1_1	230	234	PF00082	0.219
CLV_PCSK_SKI1_1	275	279	PF00082	0.325
CLV_PCSK_SKI1_1	31	35	PF00082	0.390
DEG_APCC_DBOX_1	274	282	PF00400	0.266
DEG_Nend_UBRbox_3	1	3	PF02207	0.564
DEG_SPOP_SBC_1	389	393	PF00917	0.457
DOC_CDC14_PxL_1	278	286	PF14671	0.266
DOC_MAPK_DCC_7	333	343	PF00069	0.447
DOC_MAPK_DCC_7	456	464	PF00069	0.554
DOC_MAPK_gen_1	14	21	PF00069	0.445
DOC_MAPK_gen_1	246	252	PF00069	0.251
DOC_MAPK_gen_1	333	343	PF00069	0.447
DOC_MAPK_MEF2A_6	456	464	PF00069	0.554
DOC_PP1_RVXF_1	227	234	PF00149	0.282
DOC_PP1_SILK_1	31	36	PF00149	0.145
DOC_PP2B_LxvP_1	41	44	PF13499	0.260
DOC_PP4_FxxP_1	277	280	PF00568	0.279
DOC_PP4_FxxP_1	413	416	PF00568	0.591
DOC_PP4_FxxP_1	464	467	PF00568	0.550
DOC_USP7_MATH_1	104	108	PF00917	0.244
DOC_USP7_MATH_1	259	263	PF00917	0.448
DOC_USP7_MATH_1	371	375	PF00917	0.517
DOC_USP7_MATH_1	390	394	PF00917	0.707
DOC_USP7_UBL2_3	31	35	PF12436	0.390
DOC_USP7_UBL2_3	47	51	PF12436	0.390
DOC_WW_Pin1_4	350	355	PF00397	0.684
DOC_WW_Pin1_4	377	382	PF00397	0.674
DOC_WW_Pin1_4	384	389	PF00397	0.648
DOC_WW_Pin1_4	450	455	PF00397	0.654
LIG_14-3-3_CanoR_1	254	263	PF00244	0.339
LIG_14-3-3_CanoR_1	318	325	PF00244	0.585
LIG_14-3-3_CanoR_1	81	90	PF00244	0.262
LIG_ActinCP_TwfCPI_2	464	472	PF01115	0.518
LIG_AP2alpha_2	469	471	PF02296	0.535
LIG_BRCT_BRCA1_1	273	277	PF00533	0.339
LIG_BRCT_BRCA1_1	397	401	PF00533	0.587
LIG_EVH1_1	424	428	PF00568	0.437
LIG_EVH1_2	430	434	PF00568	0.523
LIG_EVH1_2	460	464	PF00568	0.556
LIG_FHA_1	16	22	PF00498	0.596
LIG_FHA_1	35	41	PF00498	0.193
LIG_FHA_2	291	297	PF00498	0.467
LIG_FHA_2	325	331	PF00498	0.658
LIG_LIR_Apic_2	274	280	PF02991	0.339
LIG_LIR_Gen_1	211	217	PF02991	0.291
LIG_LIR_Gen_1	236	243	PF02991	0.293
LIG_LIR_Gen_1	258	267	PF02991	0.301
LIG_LIR_Gen_1	84	95	PF02991	0.259
LIG_LIR_Gen_1	96	104	PF02991	0.245
LIG_LIR_Nem_3	141	146	PF02991	0.309
LIG_LIR_Nem_3	167	171	PF02991	0.280
LIG_LIR_Nem_3	211	216	PF02991	0.281
LIG_LIR_Nem_3	236	241	PF02991	0.275
LIG_LIR_Nem_3	251	256	PF02991	0.260
LIG_LIR_Nem_3	258	263	PF02991	0.255
LIG_LIR_Nem_3	469	474	PF02991	0.458
LIG_LIR_Nem_3	56	60	PF02991	0.282
LIG_LIR_Nem_3	84	90	PF02991	0.259
LIG_LIR_Nem_3	96	100	PF02991	0.245
LIG_LYPXL_yS_3	448	451	PF13949	0.590
LIG_MYND_1	282	286	PF01753	0.279
LIG_Pex14_1	249	253	PF04695	0.245
LIG_SH2_CRK	115	119	PF00017	0.309
LIG_SH2_CRK	213	217	PF00017	0.253
LIG_SH2_NCK_1	256	260	PF00017	0.304
LIG_SH2_STAP1	256	260	PF00017	0.304
LIG_SH2_STAT5	12	15	PF00017	0.475
LIG_SH2_STAT5	138	141	PF00017	0.258
LIG_SH2_STAT5	146	149	PF00017	0.231
LIG_SH2_STAT5	171	174	PF00017	0.281
LIG_SH2_STAT5	266	269	PF00017	0.426
LIG_SH3_1	331	337	PF00018	0.650
LIG_SH3_2	458	463	PF14604	0.519
LIG_SH3_2	467	472	PF14604	0.535
LIG_SH3_3	115	121	PF00018	0.211
LIG_SH3_3	331	337	PF00018	0.559
LIG_SH3_3	351	357	PF00018	0.557
LIG_SH3_3	383	389	PF00018	0.552
LIG_SH3_3	422	428	PF00018	0.606
LIG_SH3_3	435	441	PF00018	0.616
LIG_SH3_3	448	454	PF00018	0.551
LIG_SH3_3	455	461	PF00018	0.702
LIG_SH3_3	464	470	PF00018	0.583
LIG_SUMO_SIM_par_1	87	93	PF11976	0.260
LIG_TRAF2_1	367	370	PF00917	0.562
LIG_UBA3_1	223	229	PF00899	0.390
LIG_WRC_WIRS_1	21	26	PF05994	0.444
LIG_WRC_WIRS_1	94	99	PF05994	0.308
LIG_WW_3	333	337	PF00397	0.700
MOD_CDC14_SPxK_1	453	456	PF00782	0.512
MOD_CDK_SPxK_1	450	456	PF00069	0.674
MOD_CK1_1	22	28	PF00069	0.508
MOD_CK1_1	290	296	PF00069	0.283
MOD_CK1_1	3	9	PF00069	0.616
MOD_CK1_1	320	326	PF00069	0.569
MOD_CK1_1	380	386	PF00069	0.501
MOD_CK2_1	104	110	PF00069	0.467
MOD_CK2_1	22	28	PF00069	0.356
MOD_CK2_1	290	296	PF00069	0.339
MOD_CK2_1	324	330	PF00069	0.694
MOD_Cter_Amidation	159	162	PF01082	0.390
MOD_GlcNHglycan	154	157	PF01048	0.310
MOD_GlcNHglycan	24	27	PF01048	0.492
MOD_GlcNHglycan	257	260	PF01048	0.337
MOD_GlcNHglycan	434	437	PF01048	0.699
MOD_GlcNHglycan	61	64	PF01048	0.372
MOD_GlcNHglycan	8	11	PF01048	0.629
MOD_GSK3_1	104	111	PF00069	0.370
MOD_GSK3_1	15	22	PF00069	0.483
MOD_GSK3_1	255	262	PF00069	0.298
MOD_GSK3_1	266	273	PF00069	0.220
MOD_GSK3_1	29	36	PF00069	0.179
MOD_GSK3_1	320	327	PF00069	0.557
MOD_GSK3_1	371	378	PF00069	0.646
MOD_GSK3_1	380	387	PF00069	0.614
MOD_N-GLC_1	1	6	PF02516	0.655
MOD_N-GLC_2	206	208	PF02516	0.260
MOD_NEK2_1	108	113	PF00069	0.362
MOD_NEK2_1	271	276	PF00069	0.294
MOD_NEK2_1	34	39	PF00069	0.277
MOD_NEK2_2	259	264	PF00069	0.390
MOD_NEK2_2	390	395	PF00069	0.463
MOD_PIKK_1	138	144	PF00454	0.255
MOD_PIKK_1	365	371	PF00454	0.490
MOD_PKA_2	299	305	PF00069	0.410
MOD_PKA_2	317	323	PF00069	0.535
MOD_Plk_1	320	326	PF00069	0.661
MOD_Plk_4	104	110	PF00069	0.260
MOD_Plk_4	266	272	PF00069	0.283
MOD_Plk_4	287	293	PF00069	0.279
MOD_Plk_4	29	35	PF00069	0.352
MOD_Plk_4	90	96	PF00069	0.260
MOD_ProDKin_1	350	356	PF00069	0.681
MOD_ProDKin_1	377	383	PF00069	0.674
MOD_ProDKin_1	384	390	PF00069	0.649
MOD_ProDKin_1	450	456	PF00069	0.650
MOD_SUMO_rev_2	3	9	PF00179	0.505
MOD_SUMO_rev_2	323	332	PF00179	0.673
TRG_DiLeu_BaEn_1	211	216	PF01217	0.390
TRG_DiLeu_BaLyEn_6	96	101	PF01217	0.356
TRG_ENDOCYTIC_2	115	118	PF00928	0.390
TRG_ENDOCYTIC_2	213	216	PF00928	0.264
TRG_ENDOCYTIC_2	448	451	PF00928	0.590
TRG_ER_diArg_1	161	163	PF00400	0.388
TRG_ER_diArg_1	247	249	PF00400	0.327
TRG_ER_diArg_1	253	255	PF00400	0.286
TRG_ER_diArg_1	317	319	PF00400	0.482
TRG_Pf-PMV_PEXEL_1	145	149	PF00026	0.189
TRG_Pf-PMV_PEXEL_1	150	154	PF00026	0.256
TRG_Pf-PMV_PEXEL_1	285	289	PF00026	0.340
TRG_Pf-PMV_PEXEL_1	99	103	PF00026	0.309

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1II26	Leptomonas seymouri	59%	100%
A0A0S4J0L1	Bodo saltans	38%	100%
A0A1X0NIY0	Trypanosomatidae	42%	97%
A0A3S5H7C6	Leishmania donovani	87%	100%
A0A422P449	Trypanosoma rangeli	38%	96%
A4HD60	Leishmania braziliensis	72%	99%
A4I0M5	Leishmania infantum	87%	100%
D0A6X3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	98%
Q13492	Homo sapiens	21%	74%
Q4QAX8	Leishmania major	84%	100%
Q7M6Y3	Mus musculus	21%	73%
Q8LBH2	Arabidopsis thaliana	24%	85%
Q8VYT2	Arabidopsis thaliana	22%	80%
Q9LHS0	Arabidopsis thaliana	24%	89%
Q9LVD8	Arabidopsis thaliana	24%	82%
V5DSQ8	Trypanosoma cruzi	44%	97%