GAF domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

GAF domain/TIP41-like family, putative

Species:

Leishmania mexicana

UniProt:

E9AWH8_LEIMU

TriTrypDb:

LmxM.23.1460

Length:

522

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0005829	cytosol	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AWH8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AWH8

Function

Biological processes
Term	Name	Level	Count
GO:0010921	regulation of phosphatase activity	5	11
GO:0019220	regulation of phosphate metabolic process	6	11
GO:0019222	regulation of metabolic process	3	11
GO:0031323	regulation of cellular metabolic process	4	11
GO:0031399	regulation of protein modification process	6	11
GO:0035303	regulation of dephosphorylation	7	11
GO:0035304	regulation of protein dephosphorylation	7	11
GO:0043666	regulation of phosphoprotein phosphatase activity	6	11
GO:0050789	regulation of biological process	2	11
GO:0050790	regulation of catalytic activity	3	11
GO:0050794	regulation of cellular process	3	11
GO:0051171	regulation of nitrogen compound metabolic process	4	11
GO:0051174	regulation of phosphorus metabolic process	5	11
GO:0051246	regulation of protein metabolic process	5	11
GO:0051336	regulation of hydrolase activity	4	11
GO:0060255	regulation of macromolecule metabolic process	4	11
GO:0065007	biological regulation	1	11
GO:0065009	regulation of molecular function	2	11
GO:0080090	regulation of primary metabolic process	4	11

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	1
GO:0016491	oxidoreductase activity	2	1
GO:0016667	oxidoreductase activity, acting on a sulfur group of donors	3	1
GO:0016671	oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor	4	1
GO:0070191	methionine-R-sulfoxide reductase activity	5	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	385	389	PF00656	0.478
CLV_PCSK_KEX2_1	14	16	PF00082	0.265
CLV_PCSK_KEX2_1	34	36	PF00082	0.537
CLV_PCSK_PC1ET2_1	14	16	PF00082	0.265
CLV_PCSK_PC1ET2_1	34	36	PF00082	0.537
CLV_PCSK_SKI1_1	14	18	PF00082	0.271
CLV_PCSK_SKI1_1	271	275	PF00082	0.403
CLV_PCSK_SKI1_1	34	38	PF00082	0.479
CLV_PCSK_SKI1_1	518	522	PF00082	0.656
DEG_APCC_DBOX_1	265	273	PF00400	0.427
DEG_SPOP_SBC_1	233	237	PF00917	0.512
DOC_CDC14_PxL_1	79	87	PF14671	0.411
DOC_CYCLIN_yCln2_LP_2	341	347	PF00134	0.478
DOC_MAPK_DCC_7	466	476	PF00069	0.417
DOC_MAPK_MEF2A_6	136	145	PF00069	0.411
DOC_MAPK_MEF2A_6	308	315	PF00069	0.393
DOC_MAPK_MEF2A_6	334	343	PF00069	0.483
DOC_MAPK_MEF2A_6	398	406	PF00069	0.456
DOC_PP1_RVXF_1	358	364	PF00149	0.489
DOC_PP1_RVXF_1	69	75	PF00149	0.458
DOC_PP2B_LxvP_1	197	200	PF13499	0.577
DOC_PP2B_LxvP_1	341	344	PF13499	0.446
DOC_PP4_FxxP_1	60	63	PF00568	0.445
DOC_PP4_FxxP_1	80	83	PF00568	0.145
DOC_USP7_MATH_1	10	14	PF00917	0.333
DOC_USP7_MATH_1	130	134	PF00917	0.281
DOC_USP7_UBL2_3	246	250	PF12436	0.584
DOC_USP7_UBL2_3	356	360	PF12436	0.544
DOC_WW_Pin1_4	3	8	PF00397	0.570
DOC_WW_Pin1_4	300	305	PF00397	0.447
DOC_WW_Pin1_4	34	39	PF00397	0.421
DOC_WW_Pin1_4	464	469	PF00397	0.537
DOC_WW_Pin1_4	495	500	PF00397	0.454
LIG_14-3-3_CanoR_1	342	352	PF00244	0.562
LIG_14-3-3_CanoR_1	430	440	PF00244	0.530
LIG_14-3-3_CanoR_1	94	102	PF00244	0.360
LIG_14-3-3_CterR_2	518	522	PF00244	0.377
LIG_Actin_WH2_2	468	483	PF00022	0.397
LIG_APCC_ABBA_1	212	217	PF00400	0.748
LIG_APCC_ABBA_1	456	461	PF00400	0.456
LIG_BIR_III_4	388	392	PF00653	0.478
LIG_BRCT_BRCA1_1	32	36	PF00533	0.515
LIG_BRCT_BRCA1_1	350	354	PF00533	0.478
LIG_BRCT_BRCA1_1	432	436	PF00533	0.552
LIG_BRCT_BRCA1_2	350	356	PF00533	0.478
LIG_deltaCOP1_diTrp_1	309	315	PF00928	0.494
LIG_deltaCOP1_diTrp_1	362	369	PF00928	0.466
LIG_deltaCOP1_diTrp_1	450	459	PF00928	0.446
LIG_EH1_1	314	322	PF00400	0.460
LIG_FHA_1	234	240	PF00498	0.507
LIG_FHA_1	254	260	PF00498	0.397
LIG_FHA_1	297	303	PF00498	0.367
LIG_FHA_1	422	428	PF00498	0.522
LIG_FHA_1	505	511	PF00498	0.388
LIG_FHA_2	107	113	PF00498	0.331
LIG_FHA_2	265	271	PF00498	0.417
LIG_FHA_2	272	278	PF00498	0.389
LIG_FHA_2	333	339	PF00498	0.377
LIG_FXI_DFP_1	182	186	PF00024	0.458
LIG_FXI_DFP_1	407	411	PF00024	0.278
LIG_IRF3_LxIS_1	281	288	PF10401	0.434
LIG_LIR_Gen_1	309	320	PF02991	0.471
LIG_LIR_Gen_1	346	357	PF02991	0.464
LIG_LIR_Gen_1	409	417	PF02991	0.478
LIG_LIR_Gen_1	434	444	PF02991	0.466
LIG_LIR_LC3C_4	115	119	PF02991	0.332
LIG_LIR_Nem_3	309	315	PF02991	0.485
LIG_LIR_Nem_3	330	336	PF02991	0.484
LIG_LIR_Nem_3	346	352	PF02991	0.464
LIG_LIR_Nem_3	433	439	PF02991	0.444
LIG_MLH1_MIPbox_1	433	437	PF16413	0.446
LIG_Pex14_1	365	369	PF04695	0.431
LIG_Pex14_1	74	78	PF04695	0.266
LIG_Pex14_2	406	410	PF04695	0.456
LIG_Pex14_2	436	440	PF04695	0.456
LIG_REV1ctd_RIR_1	242	250	PF16727	0.557
LIG_SH2_CRK	202	206	PF00017	0.687
LIG_SH2_STAP1	18	22	PF00017	0.393
LIG_SH2_STAP1	202	206	PF00017	0.687
LIG_SH2_STAT3	78	81	PF00017	0.300
LIG_SH2_STAT5	78	81	PF00017	0.279
LIG_SH3_3	377	383	PF00018	0.446
LIG_SUMO_SIM_anti_2	149	155	PF11976	0.331
LIG_SUMO_SIM_par_1	141	146	PF11976	0.337
LIG_SUMO_SIM_par_1	149	155	PF11976	0.252
LIG_SUMO_SIM_par_1	298	303	PF11976	0.397
LIG_SUMO_SIM_par_1	506	511	PF11976	0.383
LIG_SxIP_EBH_1	235	246	PF03271	0.567
LIG_SxIP_EBH_1	93	104	PF03271	0.167
MOD_CK1_1	113	119	PF00069	0.381
MOD_CK2_1	106	112	PF00069	0.441
MOD_CK2_1	132	138	PF00069	0.226
MOD_CK2_1	271	277	PF00069	0.433
MOD_CK2_1	320	326	PF00069	0.416
MOD_GlcNHglycan	112	115	PF01048	0.329
MOD_GlcNHglycan	132	135	PF01048	0.145
MOD_GlcNHglycan	202	205	PF01048	0.619
MOD_GlcNHglycan	287	290	PF01048	0.445
MOD_GlcNHglycan	322	325	PF01048	0.350
MOD_GlcNHglycan	345	348	PF01048	0.381
MOD_GlcNHglycan	433	436	PF01048	0.430
MOD_GlcNHglycan	468	471	PF01048	0.456
MOD_GlcNHglycan	502	505	PF01048	0.506
MOD_GSK3_1	106	113	PF00069	0.437
MOD_GSK3_1	130	137	PF00069	0.309
MOD_GSK3_1	225	232	PF00069	0.674
MOD_GSK3_1	233	240	PF00069	0.531
MOD_GSK3_1	281	288	PF00069	0.474
MOD_GSK3_1	296	303	PF00069	0.387
MOD_GSK3_1	30	37	PF00069	0.476
MOD_GSK3_1	462	469	PF00069	0.429
MOD_GSK3_1	500	507	PF00069	0.523
MOD_N-GLC_1	146	151	PF02516	0.411
MOD_N-GLC_2	368	370	PF02516	0.287
MOD_NEK2_1	264	269	PF00069	0.428
MOD_NEK2_1	281	286	PF00069	0.355
MOD_NEK2_1	320	325	PF00069	0.439
MOD_NEK2_1	397	402	PF00069	0.364
MOD_NEK2_1	422	427	PF00069	0.473
MOD_NEK2_1	462	467	PF00069	0.316
MOD_NEK2_2	348	353	PF00069	0.287
MOD_PIKK_1	271	277	PF00454	0.403
MOD_PK_1	298	304	PF00069	0.357
MOD_Plk_1	398	404	PF00069	0.276
MOD_Plk_2-3	332	338	PF00069	0.297
MOD_Plk_4	113	119	PF00069	0.319
MOD_Plk_4	146	152	PF00069	0.411
MOD_Plk_4	332	338	PF00069	0.599
MOD_Plk_4	504	510	PF00069	0.444
MOD_ProDKin_1	3	9	PF00069	0.557
MOD_ProDKin_1	300	306	PF00069	0.460
MOD_ProDKin_1	34	40	PF00069	0.415
MOD_ProDKin_1	464	470	PF00069	0.414
MOD_ProDKin_1	495	501	PF00069	0.449
MOD_SUMO_for_1	245	248	PF00179	0.564
MOD_SUMO_rev_2	276	281	PF00179	0.512
MOD_SUMO_rev_2	326	336	PF00179	0.402
MOD_SUMO_rev_2	355	361	PF00179	0.421
MOD_SUMO_rev_2	487	497	PF00179	0.561
TRG_DiLeu_BaEn_1	332	337	PF01217	0.537
TRG_DiLeu_BaEn_1	375	380	PF01217	0.232
TRG_ENDOCYTIC_2	18	21	PF00928	0.315
TRG_ENDOCYTIC_2	202	205	PF00928	0.684
TRG_ENDOCYTIC_2	407	410	PF00928	0.411
TRG_ENDOCYTIC_2	429	432	PF00928	0.331
TRG_NES_CRM1_1	170	184	PF08389	0.200
TRG_Pf-PMV_PEXEL_1	15	19	PF00026	0.354

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I5N3	Leptomonas seymouri	68%	100%
A0A0S4JL71	Bodo saltans	35%	100%
A0A1X0NWX3	Trypanosomatidae	43%	97%
A0A3S7WY26	Leishmania donovani	93%	100%
A0A422NJH6	Trypanosoma rangeli	44%	94%
A4HD30	Leishmania braziliensis	83%	100%
A4I0L7	Leishmania infantum	93%	100%
C9ZNQ7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	41%	91%
Q4QAZ2	Leishmania major	94%	100%
V5BB88	Trypanosoma cruzi	46%	95%