5-formyltetrahydrofolate cyclo-ligase family protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

5-formyltetrahydrofolate cyclo-ligase family, putative

Species:

Leishmania mexicana

UniProt:

E9AWC2_LEIMU

TriTrypDb:

LmxM.23.1045

Length:

449

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005739	mitochondrion	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AWC2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AWC2

Function

Biological processes
Term	Name	Level	Count
GO:0006575	cellular modified amino acid metabolic process	3	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006730	one-carbon metabolic process	3	1
GO:0006760	folic acid-containing compound metabolic process	4	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009058	biosynthetic process	2	1
GO:0009396	folic acid-containing compound biosynthetic process	5	1
GO:0009987	cellular process	1	1
GO:0018130	heterocycle biosynthetic process	4	1
GO:0019438	aromatic compound biosynthetic process	4	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0035999	tetrahydrofolate interconversion	4	1
GO:0042398	cellular modified amino acid biosynthetic process	4	1
GO:0042558	pteridine-containing compound metabolic process	4	1
GO:0042559	pteridine-containing compound biosynthetic process	5	1
GO:0044237	cellular metabolic process	2	1
GO:0044249	cellular biosynthetic process	3	1
GO:0044271	cellular nitrogen compound biosynthetic process	4	1
GO:0044281	small molecule metabolic process	2	1
GO:0046483	heterocycle metabolic process	3	1
GO:0046653	tetrahydrofolate metabolic process	5	1
GO:0071704	organic substance metabolic process	2	1
GO:1901360	organic cyclic compound metabolic process	3	1
GO:1901362	organic cyclic compound biosynthetic process	4	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1901566	organonitrogen compound biosynthetic process	4	1
GO:1901576	organic substance biosynthetic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	2
GO:0016874	ligase activity	2	2
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	1
GO:0016882	cyclo-ligase activity	4	1
GO:0030272	5-formyltetrahydrofolate cyclo-ligase activity	5	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	361	365	PF00656	0.427
CLV_C14_Caspase3-7	71	75	PF00656	0.423
CLV_MEL_PAP_1	353	359	PF00089	0.538
CLV_NRD_NRD_1	13	15	PF00675	0.352
CLV_NRD_NRD_1	22	24	PF00675	0.350
CLV_NRD_NRD_1	346	348	PF00675	0.372
CLV_PCSK_FUR_1	20	24	PF00082	0.426
CLV_PCSK_KEX2_1	22	24	PF00082	0.408
CLV_PCSK_KEX2_1	345	347	PF00082	0.375
CLV_PCSK_SKI1_1	10	14	PF00082	0.369
CLV_PCSK_SKI1_1	23	27	PF00082	0.399
DEG_Nend_UBRbox_3	1	3	PF02207	0.578
DEG_SPOP_SBC_1	399	403	PF00917	0.310
DEG_SPOP_SBC_1	62	66	PF00917	0.486
DOC_CKS1_1	118	123	PF01111	0.454
DOC_CYCLIN_yCln2_LP_2	202	208	PF00134	0.482
DOC_CYCLIN_yCln2_LP_2	77	83	PF00134	0.468
DOC_MAPK_gen_1	10	21	PF00069	0.503
DOC_MAPK_gen_1	246	254	PF00069	0.316
DOC_MAPK_MEF2A_6	169	177	PF00069	0.476
DOC_MAPK_MEF2A_6	302	311	PF00069	0.367
DOC_MAPK_RevD_3	7	23	PF00069	0.428
DOC_PP2B_LxvP_1	77	80	PF13499	0.598
DOC_PP2B_LxvP_1	81	84	PF13499	0.470
DOC_PP2B_PxIxI_1	409	415	PF00149	0.519
DOC_USP7_MATH_1	300	304	PF00917	0.456
DOC_USP7_MATH_1	33	37	PF00917	0.362
DOC_USP7_MATH_1	349	353	PF00917	0.533
DOC_USP7_MATH_1	365	369	PF00917	0.545
DOC_USP7_MATH_1	405	409	PF00917	0.629
DOC_USP7_MATH_1	62	66	PF00917	0.486
DOC_USP7_UBL2_3	11	15	PF12436	0.505
DOC_WW_Pin1_4	117	122	PF00397	0.356
DOC_WW_Pin1_4	142	147	PF00397	0.766
DOC_WW_Pin1_4	148	153	PF00397	0.691
DOC_WW_Pin1_4	185	190	PF00397	0.504
DOC_WW_Pin1_4	221	226	PF00397	0.617
DOC_WW_Pin1_4	287	292	PF00397	0.437
DOC_WW_Pin1_4	373	378	PF00397	0.626
DOC_WW_Pin1_4	434	439	PF00397	0.417
DOC_WW_Pin1_4	79	84	PF00397	0.573
LIG_14-3-3_CanoR_1	212	220	PF00244	0.492
LIG_14-3-3_CanoR_1	23	29	PF00244	0.486
LIG_14-3-3_CanoR_1	60	67	PF00244	0.613
LIG_AP2alpha_1	332	336	PF02296	0.492
LIG_APCC_ABBA_1	92	97	PF00400	0.327
LIG_BIR_III_2	374	378	PF00653	0.493
LIG_BRCT_BRCA1_1	351	355	PF00533	0.390
LIG_eIF4E_1	270	276	PF01652	0.501
LIG_FHA_1	118	124	PF00498	0.395
LIG_FHA_1	213	219	PF00498	0.654
LIG_FHA_1	296	302	PF00498	0.456
LIG_FHA_1	4	10	PF00498	0.428
LIG_FHA_1	54	60	PF00498	0.358
LIG_HCF-1_HBM_1	41	44	PF13415	0.452
LIG_LIR_Gen_1	236	243	PF02991	0.370
LIG_LIR_Gen_1	256	263	PF02991	0.408
LIG_LIR_Gen_1	277	285	PF02991	0.350
LIG_LIR_Gen_1	41	51	PF02991	0.304
LIG_LIR_Nem_3	236	242	PF02991	0.378
LIG_LIR_Nem_3	256	261	PF02991	0.409
LIG_LIR_Nem_3	277	281	PF02991	0.361
LIG_LIR_Nem_3	334	339	PF02991	0.451
LIG_LIR_Nem_3	41	47	PF02991	0.321
LIG_MYND_2	84	88	PF01753	0.201
LIG_Pex14_2	332	336	PF04695	0.492
LIG_SH2_CRK	239	243	PF00017	0.356
LIG_SH2_CRK	258	262	PF00017	0.423
LIG_SH2_NCK_1	278	282	PF00017	0.338
LIG_SH2_SRC	44	47	PF00017	0.432
LIG_SH2_STAP1	239	243	PF00017	0.356
LIG_SH2_STAP1	258	262	PF00017	0.423
LIG_SH2_STAP1	333	337	PF00017	0.440
LIG_SH2_STAP1	44	48	PF00017	0.431
LIG_SH2_STAT5	46	49	PF00017	0.394
LIG_SH2_STAT5	91	94	PF00017	0.346
LIG_SH2_STAT5	95	98	PF00017	0.348
LIG_SH3_3	119	125	PF00018	0.374
LIG_SH3_3	154	160	PF00018	0.745
LIG_SH3_3	215	221	PF00018	0.476
LIG_SH3_3	308	314	PF00018	0.471
LIG_SH3_3	354	360	PF00018	0.420
LIG_SH3_3	380	386	PF00018	0.450
LIG_SH3_3	432	438	PF00018	0.397
LIG_SH3_3	77	83	PF00018	0.511
LIG_SUMO_SIM_anti_2	305	311	PF11976	0.367
LIG_TYR_ITIM	276	281	PF00017	0.350
LIG_TYR_ITIM	89	94	PF00017	0.370
LIG_UBA3_1	18	26	PF00899	0.492
MOD_CK1_1	211	217	PF00069	0.612
MOD_CK1_1	224	230	PF00069	0.720
MOD_CK1_1	279	285	PF00069	0.418
MOD_CK1_1	401	407	PF00069	0.607
MOD_CK1_1	415	421	PF00069	0.704
MOD_CK1_1	63	69	PF00069	0.678
MOD_CK2_1	198	204	PF00069	0.538
MOD_CK2_1	223	229	PF00069	0.587
MOD_GlcNHglycan	169	172	PF01048	0.406
MOD_GlcNHglycan	272	275	PF01048	0.469
MOD_GlcNHglycan	33	36	PF01048	0.278
MOD_GlcNHglycan	380	383	PF01048	0.484
MOD_GlcNHglycan	407	410	PF01048	0.488
MOD_GlcNHglycan	414	417	PF01048	0.456
MOD_GSK3_1	126	133	PF00069	0.506
MOD_GSK3_1	208	215	PF00069	0.508
MOD_GSK3_1	279	286	PF00069	0.582
MOD_GSK3_1	400	407	PF00069	0.619
MOD_N-GLC_1	198	203	PF02516	0.376
MOD_NEK2_1	198	203	PF00069	0.494
MOD_NEK2_1	241	246	PF00069	0.462
MOD_NEK2_1	378	383	PF00069	0.495
MOD_NEK2_1	400	405	PF00069	0.568
MOD_NEK2_2	339	344	PF00069	0.496
MOD_PIKK_1	33	39	PF00454	0.498
MOD_PKA_2	211	217	PF00069	0.527
MOD_PKA_2	270	276	PF00069	0.535
MOD_Plk_1	198	204	PF00069	0.381
MOD_Plk_1	305	311	PF00069	0.540
MOD_Plk_1	73	79	PF00069	0.507
MOD_Plk_4	198	204	PF00069	0.516
MOD_Plk_4	305	311	PF00069	0.542
MOD_ProDKin_1	117	123	PF00069	0.364
MOD_ProDKin_1	142	148	PF00069	0.614
MOD_ProDKin_1	185	191	PF00069	0.512
MOD_ProDKin_1	221	227	PF00069	0.619
MOD_ProDKin_1	287	293	PF00069	0.437
MOD_ProDKin_1	373	379	PF00069	0.623
MOD_ProDKin_1	434	440	PF00069	0.422
MOD_ProDKin_1	79	85	PF00069	0.560
MOD_SUMO_for_1	25	28	PF00179	0.438
TRG_DiLeu_BaLyEn_6	82	87	PF01217	0.479
TRG_ENDOCYTIC_2	239	242	PF00928	0.367
TRG_ENDOCYTIC_2	258	261	PF00928	0.465
TRG_ENDOCYTIC_2	278	281	PF00928	0.309
TRG_ENDOCYTIC_2	333	336	PF00928	0.446
TRG_ENDOCYTIC_2	44	47	PF00928	0.321
TRG_ENDOCYTIC_2	91	94	PF00928	0.306
TRG_ENDOCYTIC_2	95	98	PF00928	0.300
TRG_ER_diArg_1	19	22	PF00400	0.433
TRG_ER_diArg_1	345	347	PF00400	0.425
TRG_Pf-PMV_PEXEL_1	302	306	PF00026	0.225
TRG_Pf-PMV_PEXEL_1	346	351	PF00026	0.410

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I1K4	Leptomonas seymouri	41%	100%
A0A1X0NWU1	Trypanosomatidae	27%	100%
A0A3Q8IEK9	Leishmania donovani	86%	100%
A0A3R7KPU1	Trypanosoma rangeli	28%	100%
A4HCX6	Leishmania braziliensis	71%	100%
A4I0F9	Leishmania infantum	86%	100%
C9ZV81	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	33%	100%
Q4QB50	Leishmania major	85%	100%
V5DFL5	Trypanosoma cruzi	29%	100%