PriCT_2 domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

mitochondrial DNA primase, putative

Species:

Leishmania mexicana

UniProt:

E9AW83_LEIMU

TriTrypDb:

LmxM.23.0680

Length:

535

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005654	nucleoplasm	2	1
GO:0005730	nucleolus	5	1
GO:0005759	mitochondrial matrix	5	1
GO:0020023	kinetoplast	2	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0043226	organelle	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AW83

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AW83

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006259	DNA metabolic process	4	1
GO:0006260	DNA replication	5	1
GO:0006261	DNA-templated DNA replication	6	1
GO:0006264	mitochondrial DNA replication	6	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009987	cellular process	1	1
GO:0032042	mitochondrial DNA metabolic process	5	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0043170	macromolecule metabolic process	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0016787	hydrolase activity	2	11
GO:0016817	hydrolase activity, acting on acid anhydrides	3	11
GO:0003896	DNA primase activity	7	1
GO:0003899	DNA-directed 5'-3' RNA polymerase activity	6	4
GO:0016740	transferase activity	2	4
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	4
GO:0016779	nucleotidyltransferase activity	4	4
GO:0034062	5'-3' RNA polymerase activity	5	4
GO:0097747	RNA polymerase activity	4	4
GO:0140098	catalytic activity, acting on RNA	3	4
GO:0140640	catalytic activity, acting on a nucleic acid	2	4

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	120	124	PF00656	0.349
CLV_C14_Caspase3-7	421	425	PF00656	0.295
CLV_NRD_NRD_1	101	103	PF00675	0.447
CLV_NRD_NRD_1	128	130	PF00675	0.277
CLV_NRD_NRD_1	197	199	PF00675	0.409
CLV_NRD_NRD_1	430	432	PF00675	0.393
CLV_NRD_NRD_1	5	7	PF00675	0.623
CLV_NRD_NRD_1	522	524	PF00675	0.709
CLV_NRD_NRD_1	533	535	PF00675	0.709
CLV_PCSK_KEX2_1	128	130	PF00082	0.295
CLV_PCSK_KEX2_1	465	467	PF00082	0.520
CLV_PCSK_KEX2_1	533	535	PF00082	0.724
CLV_PCSK_PC1ET2_1	465	467	PF00082	0.461
CLV_PCSK_SKI1_1	198	202	PF00082	0.311
CLV_PCSK_SKI1_1	328	332	PF00082	0.455
CLV_PCSK_SKI1_1	381	385	PF00082	0.349
CLV_PCSK_SKI1_1	523	527	PF00082	0.706
CLV_PCSK_SKI1_1	529	533	PF00082	0.708
DEG_APCC_DBOX_1	197	205	PF00400	0.433
DEG_APCC_KENBOX_2	283	287	PF00400	0.388
DEG_Nend_Nbox_1	1	3	PF02207	0.581
DEG_SIAH_1	495	503	PF03145	0.472
DOC_ANK_TNKS_1	347	354	PF00023	0.456
DOC_ANK_TNKS_1	5	12	PF00023	0.717
DOC_CKS1_1	141	146	PF01111	0.295
DOC_CYCLIN_RxL_1	325	333	PF00134	0.388
DOC_CYCLIN_yCln2_LP_2	358	361	PF00134	0.407
DOC_MAPK_DCC_7	258	268	PF00069	0.365
DOC_MAPK_DCC_7	409	419	PF00069	0.174
DOC_MAPK_gen_1	284	294	PF00069	0.550
DOC_MAPK_gen_1	378	388	PF00069	0.405
DOC_MAPK_gen_1	431	437	PF00069	0.409
DOC_MAPK_gen_1	465	472	PF00069	0.468
DOC_MAPK_HePTP_8	165	177	PF00069	0.295
DOC_MAPK_MEF2A_6	168	177	PF00069	0.295
DOC_MAPK_MEF2A_6	262	270	PF00069	0.367
DOC_MAPK_MEF2A_6	392	399	PF00069	0.277
DOC_MAPK_MEF2A_6	412	419	PF00069	0.150
DOC_MAPK_MEF2A_6	465	474	PF00069	0.481
DOC_MAPK_NFAT4_5	392	400	PF00069	0.295
DOC_PP1_RVXF_1	326	333	PF00149	0.390
DOC_PP2B_LxvP_1	358	361	PF13499	0.545
DOC_PP2B_LxvP_1	470	473	PF13499	0.493
DOC_PP4_FxxP_1	138	141	PF00568	0.277
DOC_PP4_FxxP_1	294	297	PF00568	0.479
DOC_PP4_FxxP_1	332	335	PF00568	0.404
DOC_USP7_MATH_1	117	121	PF00917	0.488
DOC_USP7_MATH_1	151	155	PF00917	0.371
DOC_USP7_MATH_1	314	318	PF00917	0.338
DOC_USP7_MATH_1	485	489	PF00917	0.404
DOC_USP7_MATH_1	83	87	PF00917	0.268
DOC_USP7_MATH_1	9	13	PF00917	0.722
DOC_USP7_UBL2_3	432	436	PF12436	0.277
DOC_USP7_UBL2_3	526	530	PF12436	0.698
DOC_WW_Pin1_4	140	145	PF00397	0.319
DOC_WW_Pin1_4	168	173	PF00397	0.337
DOC_WW_Pin1_4	224	229	PF00397	0.278
DOC_WW_Pin1_4	498	503	PF00397	0.729
LIG_14-3-3_CanoR_1	102	110	PF00244	0.442
LIG_14-3-3_CanoR_1	287	294	PF00244	0.427
LIG_14-3-3_CanoR_1	328	333	PF00244	0.510
LIG_14-3-3_CanoR_1	381	388	PF00244	0.482
LIG_Actin_WH2_2	216	233	PF00022	0.295
LIG_APCC_ABBA_1	291	296	PF00400	0.340
LIG_deltaCOP1_diTrp_1	295	303	PF00928	0.371
LIG_deltaCOP1_diTrp_1	390	394	PF00928	0.338
LIG_deltaCOP1_diTrp_1	443	449	PF00928	0.277
LIG_FHA_1	102	108	PF00498	0.295
LIG_FHA_1	219	225	PF00498	0.399
LIG_FHA_1	26	32	PF00498	0.509
LIG_FHA_1	286	292	PF00498	0.433
LIG_FHA_2	169	175	PF00498	0.390
LIG_FHA_2	28	34	PF00498	0.608
LIG_FHA_2	366	372	PF00498	0.389
LIG_FHA_2	382	388	PF00498	0.461
LIG_LIR_Apic_2	331	335	PF02991	0.527
LIG_LIR_Gen_1	243	253	PF02991	0.349
LIG_LIR_Gen_1	324	334	PF02991	0.394
LIG_LIR_Gen_1	443	454	PF02991	0.277
LIG_LIR_Gen_1	87	94	PF02991	0.356
LIG_LIR_Nem_3	243	249	PF02991	0.338
LIG_LIR_Nem_3	251	256	PF02991	0.326
LIG_LIR_Nem_3	324	329	PF02991	0.388
LIG_LIR_Nem_3	33	38	PF02991	0.505
LIG_LIR_Nem_3	393	397	PF02991	0.295
LIG_LIR_Nem_3	443	449	PF02991	0.277
LIG_LIR_Nem_3	458	464	PF02991	0.277
LIG_LIR_Nem_3	87	93	PF02991	0.392
LIG_MAD2	199	207	PF02301	0.349
LIG_Pex14_1	299	303	PF04695	0.357
LIG_Pex14_1	460	464	PF04695	0.277
LIG_Pex14_2	255	259	PF04695	0.362
LIG_SH2_CRK	253	257	PF00017	0.235
LIG_SH2_CRK	464	468	PF00017	0.389
LIG_SH2_CRK	90	94	PF00017	0.311
LIG_SH2_NCK_1	90	94	PF00017	0.249
LIG_SH2_STAP1	90	94	PF00017	0.425
LIG_SH2_STAT5	38	41	PF00017	0.502
LIG_SH3_3	138	144	PF00018	0.295
LIG_SH3_3	150	156	PF00018	0.349
LIG_SH3_3	201	207	PF00018	0.349
LIG_SH3_3	317	323	PF00018	0.435
LIG_SH3_3	360	366	PF00018	0.533
LIG_SH3_3	501	507	PF00018	0.579
LIG_SUMO_SIM_anti_2	171	178	PF11976	0.320
LIG_SUMO_SIM_par_1	143	150	PF11976	0.257
LIG_TRAF2_1	12	15	PF00917	0.801
LIG_TRAF2_1	30	33	PF00917	0.570
LIG_UBA3_1	1	7	PF00899	0.713
LIG_UBA3_1	276	284	PF00899	0.333
LIG_UBA3_1	364	370	PF00899	0.376
LIG_WRC_WIRS_1	329	334	PF05994	0.453
MOD_CDK_SPxxK_3	224	231	PF00069	0.277
MOD_CK1_1	146	152	PF00069	0.314
MOD_CK1_1	25	31	PF00069	0.620
MOD_CK1_1	324	330	PF00069	0.568
MOD_CK1_1	46	52	PF00069	0.609
MOD_CK1_1	517	523	PF00069	0.657
MOD_CK1_1	98	104	PF00069	0.361
MOD_CK2_1	27	33	PF00069	0.691
MOD_CK2_1	365	371	PF00069	0.406
MOD_CK2_1	381	387	PF00069	0.277
MOD_CK2_1	71	77	PF00069	0.414
MOD_CK2_1	9	15	PF00069	0.673
MOD_CMANNOS	391	394	PF00535	0.277
MOD_DYRK1A_RPxSP_1	168	172	PF00069	0.349
MOD_GlcNHglycan	113	118	PF01048	0.253
MOD_GlcNHglycan	131	134	PF01048	0.396
MOD_GlcNHglycan	149	152	PF01048	0.409
MOD_GlcNHglycan	288	291	PF01048	0.378
MOD_GlcNHglycan	438	441	PF01048	0.312
MOD_GlcNHglycan	461	464	PF01048	0.312
MOD_GlcNHglycan	483	486	PF01048	0.406
MOD_GlcNHglycan	487	490	PF01048	0.403
MOD_GlcNHglycan	508	511	PF01048	0.670
MOD_GlcNHglycan	519	522	PF01048	0.659
MOD_GlcNHglycan	73	77	PF01048	0.446
MOD_GSK3_1	113	120	PF00069	0.226
MOD_GSK3_1	143	150	PF00069	0.274
MOD_GSK3_1	151	158	PF00069	0.348
MOD_GSK3_1	18	25	PF00069	0.816
MOD_GSK3_1	324	331	PF00069	0.491
MOD_GSK3_1	455	462	PF00069	0.414
MOD_GSK3_1	481	488	PF00069	0.414
MOD_GSK3_1	97	104	PF00069	0.353
MOD_N-GLC_1	285	290	PF02516	0.407
MOD_N-GLC_1	374	379	PF02516	0.250
MOD_N-GLC_1	455	460	PF02516	0.174
MOD_N-GLC_1	95	100	PF02516	0.423
MOD_PIKK_1	218	224	PF00454	0.277
MOD_PIKK_1	36	42	PF00454	0.578
MOD_PIKK_1	43	49	PF00454	0.613
MOD_PKA_1	231	237	PF00069	0.277
MOD_PKA_2	101	107	PF00069	0.390
MOD_PKA_2	286	292	PF00069	0.445
MOD_Plk_1	113	119	PF00069	0.174
MOD_Plk_1	374	380	PF00069	0.262
MOD_Plk_2-3	22	28	PF00069	0.796
MOD_Plk_4	248	254	PF00069	0.299
MOD_ProDKin_1	140	146	PF00069	0.319
MOD_ProDKin_1	168	174	PF00069	0.337
MOD_ProDKin_1	224	230	PF00069	0.278
MOD_ProDKin_1	498	504	PF00069	0.730
MOD_SUMO_for_1	208	211	PF00179	0.355
MOD_SUMO_rev_2	10	17	PF00179	0.635
TRG_ENDOCYTIC_2	187	190	PF00928	0.409
TRG_ENDOCYTIC_2	253	256	PF00928	0.298
TRG_ENDOCYTIC_2	464	467	PF00928	0.376
TRG_ENDOCYTIC_2	90	93	PF00928	0.343
TRG_ER_diArg_1	128	130	PF00400	0.295
TRG_ER_diArg_1	532	534	PF00400	0.650
TRG_NES_CRM1_1	213	226	PF08389	0.295
TRG_Pf-PMV_PEXEL_1	328	333	PF00026	0.421
TRG_Pf-PMV_PEXEL_1	378	382	PF00026	0.418

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I0R5	Leptomonas seymouri	63%	77%
A0A0S4IY41	Bodo saltans	44%	100%
A0A1X0NXL3	Trypanosomatidae	52%	85%
A0A3S7WXR7	Leishmania donovani	92%	100%
A4HCT9	Leishmania braziliensis	83%	100%
A4I0B9	Leishmania infantum	92%	78%
C9ZV17	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	61%	85%
Q4QB95	Leishmania major	90%	99%
V5D725	Trypanosoma cruzi	59%	86%