Tryptophan--tRNA ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tryptophanyl-tRNA synthetase, putative

Species:

Leishmania mexicana

UniProt:

E9AW33_LEIMU

TriTrypDb:

LmxM.23.0300

Length:

412

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 14
NetGPI	no	yes: 0, no: 14

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AW33

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AW33

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	15
GO:0006139	nucleobase-containing compound metabolic process	3	15
GO:0006399	tRNA metabolic process	7	15
GO:0006418	tRNA aminoacylation for protein translation	6	15
GO:0006436	tryptophanyl-tRNA aminoacylation	7	15
GO:0006520	amino acid metabolic process	3	15
GO:0006725	cellular aromatic compound metabolic process	3	15
GO:0006807	nitrogen compound metabolic process	2	15
GO:0008152	metabolic process	1	15
GO:0009987	cellular process	1	15
GO:0016070	RNA metabolic process	5	15
GO:0019752	carboxylic acid metabolic process	5	15
GO:0034641	cellular nitrogen compound metabolic process	3	15
GO:0034660	ncRNA metabolic process	6	15
GO:0043038	amino acid activation	4	15
GO:0043039	tRNA aminoacylation	5	15
GO:0043170	macromolecule metabolic process	3	15
GO:0043436	oxoacid metabolic process	4	15
GO:0044237	cellular metabolic process	2	15
GO:0044238	primary metabolic process	2	15
GO:0044281	small molecule metabolic process	2	15
GO:0046483	heterocycle metabolic process	3	15
GO:0071704	organic substance metabolic process	2	15
GO:0090304	nucleic acid metabolic process	4	15
GO:1901360	organic cyclic compound metabolic process	3	15
GO:1901564	organonitrogen compound metabolic process	3	15

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	15
GO:0003824	catalytic activity	1	15
GO:0004812	aminoacyl-tRNA ligase activity	4	15
GO:0004830	tryptophan-tRNA ligase activity	5	15
GO:0005488	binding	1	15
GO:0005524	ATP binding	5	15
GO:0016874	ligase activity	2	15
GO:0016875	ligase activity, forming carbon-oxygen bonds	3	15
GO:0017076	purine nucleotide binding	4	15
GO:0030554	adenyl nucleotide binding	5	15
GO:0032553	ribonucleotide binding	3	15
GO:0032555	purine ribonucleotide binding	4	15
GO:0032559	adenyl ribonucleotide binding	5	15
GO:0035639	purine ribonucleoside triphosphate binding	4	15
GO:0036094	small molecule binding	2	15
GO:0043167	ion binding	2	15
GO:0043168	anion binding	3	15
GO:0097159	organic cyclic compound binding	2	15
GO:0097367	carbohydrate derivative binding	2	15
GO:0140098	catalytic activity, acting on RNA	3	15
GO:0140101	catalytic activity, acting on a tRNA	4	15
GO:0140640	catalytic activity, acting on a nucleic acid	2	15
GO:1901265	nucleoside phosphate binding	3	15
GO:1901363	heterocyclic compound binding	2	15

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	212	216	PF00656	0.541
CLV_NRD_NRD_1	16	18	PF00675	0.594
CLV_NRD_NRD_1	236	238	PF00675	0.317
CLV_NRD_NRD_1	312	314	PF00675	0.275
CLV_NRD_NRD_1	317	319	PF00675	0.263
CLV_NRD_NRD_1	392	394	PF00675	0.354
CLV_PCSK_FUR_1	390	394	PF00082	0.502
CLV_PCSK_KEX2_1	18	20	PF00082	0.627
CLV_PCSK_KEX2_1	236	238	PF00082	0.317
CLV_PCSK_KEX2_1	317	319	PF00082	0.300
CLV_PCSK_KEX2_1	392	394	PF00082	0.385
CLV_PCSK_PC1ET2_1	18	20	PF00082	0.546
CLV_PCSK_PC7_1	313	319	PF00082	0.334
CLV_PCSK_SKI1_1	143	147	PF00082	0.257
CLV_PCSK_SKI1_1	19	23	PF00082	0.555
CLV_PCSK_SKI1_1	198	202	PF00082	0.235
CLV_PCSK_SKI1_1	219	223	PF00082	0.249
CLV_PCSK_SKI1_1	236	240	PF00082	0.209
CLV_PCSK_SKI1_1	317	321	PF00082	0.250
CLV_PCSK_SKI1_1	383	387	PF00082	0.438
CLV_PCSK_SKI1_1	392	396	PF00082	0.350
CLV_PCSK_SKI1_1	408	412	PF00082	0.332
CLV_PCSK_SKI1_1	97	101	PF00082	0.334
DEG_Nend_UBRbox_2	1	3	PF02207	0.596
DOC_CKS1_1	32	37	PF01111	0.725
DOC_MAPK_DCC_7	58	66	PF00069	0.582
DOC_MAPK_MEF2A_6	58	66	PF00069	0.582
DOC_PP1_RVXF_1	217	223	PF00149	0.525
DOC_PP1_RVXF_1	70	77	PF00149	0.352
DOC_PP4_FxxP_1	130	133	PF00568	0.459
DOC_PP4_FxxP_1	348	351	PF00568	0.501
DOC_USP7_UBL2_3	310	314	PF12436	0.531
DOC_WW_Pin1_4	170	175	PF00397	0.338
DOC_WW_Pin1_4	21	26	PF00397	0.682
DOC_WW_Pin1_4	31	36	PF00397	0.728
DOC_WW_Pin1_4	49	54	PF00397	0.460
LIG_14-3-3_CanoR_1	17	22	PF00244	0.641
LIG_14-3-3_CanoR_1	175	179	PF00244	0.474
LIG_14-3-3_CanoR_1	181	189	PF00244	0.457
LIG_14-3-3_CanoR_1	195	201	PF00244	0.307
LIG_14-3-3_CanoR_1	392	397	PF00244	0.398
LIG_AP2alpha_2	257	259	PF02296	0.482
LIG_BIR_III_2	44	48	PF00653	0.630
LIG_BRCT_BRCA1_1	231	235	PF00533	0.442
LIG_Clathr_ClatBox_1	85	89	PF01394	0.415
LIG_FHA_1	109	115	PF00498	0.459
LIG_FHA_1	121	127	PF00498	0.375
LIG_FHA_1	206	212	PF00498	0.493
LIG_FHA_1	278	284	PF00498	0.526
LIG_FHA_1	32	38	PF00498	0.672
LIG_FHA_2	137	143	PF00498	0.442
LIG_FHA_2	210	216	PF00498	0.541
LIG_FHA_2	302	308	PF00498	0.451
LIG_FHA_2	393	399	PF00498	0.365
LIG_LIR_Apic_2	128	133	PF02991	0.459
LIG_LIR_Gen_1	340	351	PF02991	0.482
LIG_LIR_Gen_1	353	363	PF02991	0.482
LIG_LIR_Gen_1	403	412	PF02991	0.434
LIG_LIR_Nem_3	232	238	PF02991	0.435
LIG_LIR_Nem_3	257	262	PF02991	0.442
LIG_LIR_Nem_3	340	346	PF02991	0.482
LIG_LIR_Nem_3	353	359	PF02991	0.482
LIG_LIR_Nem_3	403	407	PF02991	0.405
LIG_LYPXL_S_1	189	193	PF13949	0.313
LIG_LYPXL_yS_3	190	193	PF13949	0.513
LIG_MAD2	237	245	PF02301	0.533
LIG_PDZ_Class_2	407	412	PF00595	0.406
LIG_Pex14_2	231	235	PF04695	0.428
LIG_PTB_Apo_2	179	186	PF02174	0.464
LIG_PTB_Phospho_1	179	185	PF10480	0.464
LIG_REV1ctd_RIR_1	67	76	PF16727	0.373
LIG_SH2_CRK	343	347	PF00017	0.482
LIG_SH2_STAT5	102	105	PF00017	0.440
LIG_SH2_STAT5	125	128	PF00017	0.496
LIG_SH2_STAT5	345	348	PF00017	0.533
LIG_SH3_2	53	58	PF14604	0.480
LIG_SH3_3	29	35	PF00018	0.646
LIG_SH3_3	50	56	PF00018	0.652
LIG_SUMO_SIM_par_1	301	309	PF11976	0.482
LIG_SUMO_SIM_par_1	84	89	PF11976	0.376
LIG_TRAF2_1	296	299	PF00917	0.547
LIG_TYR_ITIM	341	346	PF00017	0.482
MOD_CDK_SPK_2	170	175	PF00069	0.338
MOD_CK1_1	108	114	PF00069	0.386
MOD_CK1_1	91	97	PF00069	0.448
MOD_CK2_1	136	142	PF00069	0.440
MOD_CK2_1	292	298	PF00069	0.462
MOD_GlcNHglycan	107	110	PF01048	0.259
MOD_GlcNHglycan	25	28	PF01048	0.705
MOD_GlcNHglycan	295	298	PF01048	0.256
MOD_GlcNHglycan	320	323	PF01048	0.235
MOD_GlcNHglycan	364	367	PF01048	0.295
MOD_GlcNHglycan	369	372	PF01048	0.293
MOD_GlcNHglycan	41	44	PF01048	0.721
MOD_GSK3_1	17	24	PF00069	0.640
MOD_GSK3_1	170	177	PF00069	0.467
MOD_GSK3_1	205	212	PF00069	0.501
MOD_GSK3_1	301	308	PF00069	0.502
MOD_GSK3_1	91	98	PF00069	0.518
MOD_NEK2_1	100	105	PF00069	0.514
MOD_NEK2_1	120	125	PF00069	0.415
MOD_NEK2_1	159	164	PF00069	0.479
MOD_NEK2_1	180	185	PF00069	0.452
MOD_NEK2_1	196	201	PF00069	0.377
MOD_NEK2_1	260	265	PF00069	0.480
MOD_NEK2_1	277	282	PF00069	0.383
MOD_NEK2_1	330	335	PF00069	0.548
MOD_NEK2_2	301	306	PF00069	0.496
MOD_NEK2_2	351	356	PF00069	0.479
MOD_NEK2_2	95	100	PF00069	0.413
MOD_PIKK_1	222	228	PF00454	0.513
MOD_PIKK_1	357	363	PF00454	0.541
MOD_PKA_1	17	23	PF00069	0.480
MOD_PKA_1	392	398	PF00069	0.469
MOD_PKA_2	174	180	PF00069	0.474
MOD_PKA_2	392	398	PF00069	0.502
MOD_PKB_1	17	25	PF00069	0.559
MOD_PKB_1	362	370	PF00069	0.416
MOD_PKB_1	390	398	PF00069	0.436
MOD_Plk_1	351	357	PF00069	0.502
MOD_Plk_1	88	94	PF00069	0.523
MOD_Plk_1	95	101	PF00069	0.446
MOD_Plk_2-3	403	409	PF00069	0.312
MOD_Plk_4	174	180	PF00069	0.440
MOD_Plk_4	189	195	PF00069	0.417
MOD_Plk_4	351	357	PF00069	0.488
MOD_ProDKin_1	170	176	PF00069	0.338
MOD_ProDKin_1	21	27	PF00069	0.686
MOD_ProDKin_1	31	37	PF00069	0.728
MOD_ProDKin_1	49	55	PF00069	0.461
MOD_SUMO_rev_2	140	145	PF00179	0.435
MOD_SUMO_rev_2	370	376	PF00179	0.517
TRG_DiLeu_BaEn_2	95	101	PF01217	0.413
TRG_ENDOCYTIC_2	190	193	PF00928	0.440
TRG_ENDOCYTIC_2	343	346	PF00928	0.482
TRG_ER_diArg_1	235	237	PF00400	0.517
TRG_ER_diArg_1	316	318	PF00400	0.498
TRG_NLS_MonoExtC_3	312	317	PF00514	0.472
TRG_NLS_MonoExtN_4	15	21	PF00514	0.539
TRG_NLS_MonoExtN_4	310	317	PF00514	0.515

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P3G3	Leptomonas seymouri	44%	100%
A0A0N1HXL2	Leptomonas seymouri	71%	88%
A0A0S4IN16	Bodo saltans	46%	100%
A0A0S4JC70	Bodo saltans	60%	91%
A0A1X0NGJ6	Trypanosomatidae	62%	89%
A0A1X0NZB4	Trypanosomatidae	47%	100%
A0A3R7MPN7	Trypanosoma rangeli	47%	100%
A0A3S7WXL2	Leishmania donovani	93%	100%
A0A3S7X238	Leishmania donovani	44%	100%
A0A422NW64	Trypanosoma rangeli	63%	92%
A2BLD4	Hyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5)	28%	100%
A3MX72	Pyrobaculum calidifontis (strain DSM 21063 / JCM 11548 / VA1)	36%	100%
A4HCP4	Leishmania braziliensis	81%	100%
A4HH14	Leishmania braziliensis	45%	100%
A4I070	Leishmania infantum	93%	100%
A4I443	Leishmania infantum	44%	100%
A4WL99	Pyrobaculum arsenaticum (strain DSM 13514 / JCM 11321 / PZ6)	36%	100%
B6YUH1	Thermococcus onnurineus (strain NA1)	40%	100%
C6A032	Thermococcus sibiricus (strain DSM 12597 / MM 739)	38%	100%
C9ZKR9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	49%	100%
E9ADK8	Leishmania major	45%	100%
E9AM73	Leishmania mexicana (strain MHOM/GT/2001/U1103)	44%	100%
O26352	Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)	28%	100%
O59584	Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)	40%	100%
O96771	Encephalitozoon cuniculi (strain GB-M1)	42%	100%
P17248	Bos taurus	45%	87%
P23381	Homo sapiens	45%	87%
P23612	Oryctolagus cuniculus	45%	87%
P32921	Mus musculus	45%	86%
Q09692	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	46%	100%
Q12109	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	46%	95%
Q4JBG7	Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)	42%	100%
Q4QBE4	Leishmania major	94%	100%
Q55DZ8	Dictyostelium discoideum	45%	100%
Q58810	Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)	32%	100%
Q5JEP3	Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)	40%	100%
Q5R4J1	Pongo abelii	44%	87%
Q6P7B0	Rattus norvegicus	45%	86%
Q8TYF7	Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)	29%	100%
Q8U453	Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)	40%	100%
Q8ZTU5	Pyrobaculum aerophilum (strain ATCC 51768 / DSM 7523 / JCM 9630 / CIP 104966 / NBRC 100827 / IM2)	38%	100%
Q976M1	Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)	41%	100%
Q97ZX0	Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)	41%	100%
Q9HN66	Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)	38%	100%
Q9SR15	Arabidopsis thaliana	42%	100%
Q9UY11	Pyrococcus abyssi (strain GE5 / Orsay)	40%	100%
Q9Y924	Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)	32%	100%
V5B4S5	Trypanosoma cruzi	46%	100%