EF-hand domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania mexicana

UniProt:

E9AVS4_LEIMU

TriTrypDb:

LmxM.22.0920

Length:

862

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 1, no: 5
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0032991	protein-containing complex	1	1
GO:0034702	monoatomic ion channel complex	4	1
GO:0034703	cation channel complex	5	1
GO:0034704	calcium channel complex	6	1
GO:0098796	membrane protein complex	2	1
GO:0098798	mitochondrial protein-containing complex	2	1
GO:0098800	inner mitochondrial membrane protein complex	3	1
GO:1902495	transmembrane transporter complex	3	1
GO:1990246	uniplex complex	4	1
GO:1990351	transporter complex	2	1

Expansion

Sequence features

E9AVS4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AVS4

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	7
GO:0006811	monoatomic ion transport	4	7
GO:0006812	monoatomic cation transport	5	7
GO:0006816	calcium ion transport	7	7
GO:0006851	mitochondrial calcium ion transmembrane transport	4	7
GO:0009987	cellular process	1	7
GO:0030001	metal ion transport	6	7
GO:0034220	monoatomic ion transmembrane transport	3	7
GO:0051179	localization	1	7
GO:0051234	establishment of localization	2	7
GO:0055085	transmembrane transport	2	7
GO:0070588	calcium ion transmembrane transport	6	7
GO:0098655	monoatomic cation transmembrane transport	4	7
GO:0098660	inorganic ion transmembrane transport	4	7
GO:0098662	inorganic cation transmembrane transport	5	7
GO:1990542	mitochondrial transmembrane transport	3	7
GO:0006873	intracellular monoatomic ion homeostasis	4	1
GO:0006874	intracellular calcium ion homeostasis	7	1
GO:0006875	obsolete intracellular metal ion homeostasis	6	1
GO:0019725	cellular homeostasis	2	1
GO:0030003	intracellular monoatomic cation homeostasis	5	1
GO:0036444	calcium import into the mitochondrion	5	1
GO:0042592	homeostatic process	3	1
GO:0048878	chemical homeostasis	4	1
GO:0050801	monoatomic ion homeostasis	5	1
GO:0051560	mitochondrial calcium ion homeostasis	8	1
GO:0055065	obsolete metal ion homeostasis	7	1
GO:0055074	calcium ion homeostasis	8	1
GO:0055080	monoatomic cation homeostasis	6	1
GO:0055082	intracellular chemical homeostasis	3	1
GO:0065007	biological regulation	1	1
GO:0065008	regulation of biological quality	2	1
GO:0072503	obsolete cellular divalent inorganic cation homeostasis	6	1
GO:0072507	obsolete divalent inorganic cation homeostasis	7	1
GO:0098771	inorganic ion homeostasis	6	1

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	7
GO:0005509	calcium ion binding	5	7
GO:0043167	ion binding	2	7
GO:0043169	cation binding	3	7
GO:0046872	metal ion binding	4	7

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	236	240	PF00656	0.479
CLV_C14_Caspase3-7	369	373	PF00656	0.454
CLV_C14_Caspase3-7	752	756	PF00656	0.627
CLV_C14_Caspase3-7	815	819	PF00656	0.489
CLV_NRD_NRD_1	428	430	PF00675	0.654
CLV_NRD_NRD_1	441	443	PF00675	0.481
CLV_NRD_NRD_1	5	7	PF00675	0.464
CLV_NRD_NRD_1	506	508	PF00675	0.480
CLV_NRD_NRD_1	613	615	PF00675	0.474
CLV_NRD_NRD_1	621	623	PF00675	0.520
CLV_NRD_NRD_1	805	807	PF00675	0.391
CLV_PCSK_FUR_1	3	7	PF00082	0.417
CLV_PCSK_KEX2_1	197	199	PF00082	0.372
CLV_PCSK_KEX2_1	428	430	PF00082	0.654
CLV_PCSK_KEX2_1	441	443	PF00082	0.481
CLV_PCSK_KEX2_1	5	7	PF00082	0.464
CLV_PCSK_KEX2_1	613	615	PF00082	0.474
CLV_PCSK_KEX2_1	621	623	PF00082	0.520
CLV_PCSK_KEX2_1	805	807	PF00082	0.306
CLV_PCSK_KEX2_1	847	849	PF00082	0.571
CLV_PCSK_PC1ET2_1	197	199	PF00082	0.372
CLV_PCSK_PC1ET2_1	847	849	PF00082	0.571
CLV_PCSK_PC7_1	617	623	PF00082	0.476
CLV_PCSK_SKI1_1	5	9	PF00082	0.593
CLV_PCSK_SKI1_1	514	518	PF00082	0.549
CLV_PCSK_SKI1_1	613	617	PF00082	0.425
CLV_PCSK_SKI1_1	622	626	PF00082	0.525
CLV_PCSK_SKI1_1	666	670	PF00082	0.435
CLV_PCSK_SKI1_1	677	681	PF00082	0.476
CLV_PCSK_SKI1_1	805	809	PF00082	0.480
CLV_PCSK_SKI1_1	832	836	PF00082	0.474
CLV_Separin_Metazoa	678	682	PF03568	0.393
DEG_APCC_DBOX_1	587	595	PF00400	0.370
DEG_APCC_DBOX_1	665	673	PF00400	0.548
DEG_SCF_FBW7_1	319	325	PF00400	0.436
DEG_SCF_TRCP1_1	239	244	PF00400	0.549
DOC_CDC14_PxL_1	574	582	PF14671	0.374
DOC_CKS1_1	319	324	PF01111	0.474
DOC_CKS1_1	408	413	PF01111	0.588
DOC_CYCLIN_RxL_1	3	11	PF00134	0.461
DOC_CYCLIN_RxL_1	508	518	PF00134	0.539
DOC_CYCLIN_RxL_1	805	815	PF00134	0.479
DOC_CYCLIN_yCln2_LP_2	455	461	PF00134	0.464
DOC_CYCLIN_yCln2_LP_2	464	470	PF00134	0.436
DOC_MAPK_gen_1	3	10	PF00069	0.413
DOC_MAPK_gen_1	441	449	PF00069	0.503
DOC_MAPK_gen_1	805	812	PF00069	0.298
DOC_MAPK_MEF2A_6	660	669	PF00069	0.457
DOC_PP1_RVXF_1	590	597	PF00149	0.378
DOC_PP2B_LxvP_1	464	467	PF13499	0.424
DOC_PP4_FxxP_1	598	601	PF00568	0.314
DOC_PP4_FxxP_1	843	846	PF00568	0.495
DOC_USP7_MATH_1	156	160	PF00917	0.539
DOC_USP7_MATH_1	16	20	PF00917	0.552
DOC_USP7_MATH_1	237	241	PF00917	0.635
DOC_USP7_MATH_1	371	375	PF00917	0.605
DOC_USP7_MATH_1	381	385	PF00917	0.648
DOC_USP7_MATH_1	386	390	PF00917	0.594
DOC_USP7_MATH_1	398	402	PF00917	0.530
DOC_USP7_MATH_1	417	421	PF00917	0.749
DOC_USP7_MATH_1	422	426	PF00917	0.675
DOC_USP7_MATH_1	430	434	PF00917	0.518
DOC_USP7_MATH_1	523	527	PF00917	0.644
DOC_USP7_MATH_1	540	544	PF00917	0.430
DOC_USP7_MATH_1	554	558	PF00917	0.417
DOC_USP7_MATH_1	567	571	PF00917	0.328
DOC_USP7_MATH_1	80	84	PF00917	0.355
DOC_USP7_MATH_1	846	850	PF00917	0.386
DOC_WW_Pin1_4	318	323	PF00397	0.460
DOC_WW_Pin1_4	379	384	PF00397	0.676
DOC_WW_Pin1_4	388	393	PF00397	0.564
DOC_WW_Pin1_4	407	412	PF00397	0.633
DOC_WW_Pin1_4	487	492	PF00397	0.602
DOC_WW_Pin1_4	519	524	PF00397	0.761
DOC_WW_Pin1_4	741	746	PF00397	0.664
DOC_WW_Pin1_4	818	823	PF00397	0.401
LIG_14-3-3_CanoR_1	275	281	PF00244	0.485
LIG_14-3-3_CanoR_1	328	333	PF00244	0.410
LIG_14-3-3_CanoR_1	340	346	PF00244	0.453
LIG_14-3-3_CanoR_1	429	435	PF00244	0.554
LIG_14-3-3_CanoR_1	492	500	PF00244	0.605
LIG_14-3-3_CanoR_1	501	506	PF00244	0.496
LIG_14-3-3_CanoR_1	621	625	PF00244	0.545
LIG_14-3-3_CanoR_1	73	78	PF00244	0.532
LIG_14-3-3_CanoR_1	770	779	PF00244	0.339
LIG_14-3-3_CanoR_1	827	832	PF00244	0.381
LIG_Actin_WH2_2	577	594	PF00022	0.359
LIG_Actin_WH2_2	665	683	PF00022	0.492
LIG_Actin_WH2_2	694	709	PF00022	0.445
LIG_BIR_III_2	469	473	PF00653	0.469
LIG_BIR_III_2	670	674	PF00653	0.481
LIG_BIR_III_4	755	759	PF00653	0.485
LIG_BRCT_BRCA1_1	248	252	PF00533	0.643
LIG_BRCT_BRCA1_1	383	387	PF00533	0.584
LIG_deltaCOP1_diTrp_1	343	346	PF00928	0.439
LIG_eIF4E_1	771	777	PF01652	0.346
LIG_FHA_1	168	174	PF00498	0.545
LIG_FHA_1	350	356	PF00498	0.563
LIG_FHA_1	413	419	PF00498	0.654
LIG_FHA_1	602	608	PF00498	0.497
LIG_FHA_1	692	698	PF00498	0.442
LIG_FHA_1	725	731	PF00498	0.591
LIG_FHA_1	76	82	PF00498	0.539
LIG_FHA_2	119	125	PF00498	0.590
LIG_FHA_2	147	153	PF00498	0.589
LIG_FHA_2	158	164	PF00498	0.626
LIG_FHA_2	249	255	PF00498	0.554
LIG_FHA_2	256	262	PF00498	0.523
LIG_FHA_2	473	479	PF00498	0.674
LIG_FHA_2	750	756	PF00498	0.628
LIG_Integrin_RGD_1	617	619	PF01839	0.489
LIG_LIR_Apic_2	595	601	PF02991	0.305
LIG_LIR_Gen_1	311	319	PF02991	0.368
LIG_LIR_Gen_1	343	354	PF02991	0.361
LIG_LIR_Gen_1	783	792	PF02991	0.450
LIG_LIR_Gen_1	824	831	PF02991	0.399
LIG_LIR_Nem_3	190	195	PF02991	0.400
LIG_LIR_Nem_3	311	315	PF02991	0.388
LIG_LIR_Nem_3	343	349	PF02991	0.487
LIG_LIR_Nem_3	511	516	PF02991	0.544
LIG_LIR_Nem_3	518	524	PF02991	0.607
LIG_LIR_Nem_3	576	580	PF02991	0.382
LIG_LIR_Nem_3	644	648	PF02991	0.409
LIG_LIR_Nem_3	783	787	PF02991	0.361
LIG_LIR_Nem_3	82	88	PF02991	0.354
LIG_LIR_Nem_3	824	828	PF02991	0.299
LIG_LYPXL_yS_3	577	580	PF13949	0.377
LIG_Pex14_2	312	316	PF04695	0.233
LIG_SH2_CRK	648	652	PF00017	0.312
LIG_SH2_CRK	85	89	PF00017	0.400
LIG_SH2_STAP1	309	313	PF00017	0.333
LIG_SH2_STAP1	708	712	PF00017	0.417
LIG_SH2_STAT5	23	26	PF00017	0.569
LIG_SH2_STAT5	586	589	PF00017	0.363
LIG_SH2_STAT5	708	711	PF00017	0.419
LIG_SH2_STAT5	771	774	PF00017	0.369
LIG_SH3_3	214	220	PF00018	0.371
LIG_SH3_3	402	408	PF00018	0.576
LIG_SH3_3	572	578	PF00018	0.388
LIG_SH3_3	670	676	PF00018	0.399
LIG_SH3_3	742	748	PF00018	0.681
LIG_SUMO_SIM_par_1	163	170	PF11976	0.453
LIG_SUMO_SIM_par_1	204	210	PF11976	0.375
LIG_SUMO_SIM_par_1	215	222	PF11976	0.399
LIG_SUMO_SIM_par_1	351	356	PF11976	0.445
LIG_TRAF2_1	436	439	PF00917	0.495
LIG_TYR_ITIM	575	580	PF00017	0.370
LIG_WRC_WIRS_1	23	28	PF05994	0.463
LIG_WRC_WIRS_1	260	265	PF05994	0.393
LIG_WRC_WIRS_1	315	320	PF05994	0.363
MOD_CDK_SPK_2	487	492	PF00069	0.559
MOD_CK1_1	146	152	PF00069	0.605
MOD_CK1_1	167	173	PF00069	0.535
MOD_CK1_1	175	181	PF00069	0.511
MOD_CK1_1	27	33	PF00069	0.597
MOD_CK1_1	339	345	PF00069	0.262
MOD_CK1_1	366	372	PF00069	0.587
MOD_CK1_1	382	388	PF00069	0.602
MOD_CK1_1	433	439	PF00069	0.579
MOD_CK1_1	515	521	PF00069	0.480
MOD_CK1_1	52	58	PF00069	0.590
MOD_CK1_1	531	537	PF00069	0.624
MOD_CK1_1	557	563	PF00069	0.501
MOD_CK1_1	576	582	PF00069	0.447
MOD_CK1_1	744	750	PF00069	0.647
MOD_CK2_1	118	124	PF00069	0.531
MOD_CK2_1	157	163	PF00069	0.637
MOD_CK2_1	339	345	PF00069	0.347
MOD_CK2_1	433	439	PF00069	0.572
MOD_CK2_1	55	61	PF00069	0.579
MOD_CK2_1	73	79	PF00069	0.611
MOD_CK2_1	798	804	PF00069	0.409
MOD_CK2_1	818	824	PF00069	0.269
MOD_CK2_1	827	833	PF00069	0.411
MOD_CMANNOS	506	509	PF00535	0.597
MOD_GlcNHglycan	10	13	PF01048	0.552
MOD_GlcNHglycan	145	148	PF01048	0.633
MOD_GlcNHglycan	17	21	PF01048	0.577
MOD_GlcNHglycan	235	238	PF01048	0.630
MOD_GlcNHglycan	239	242	PF01048	0.594
MOD_GlcNHglycan	268	271	PF01048	0.482
MOD_GlcNHglycan	30	33	PF01048	0.559
MOD_GlcNHglycan	368	371	PF01048	0.676
MOD_GlcNHglycan	372	376	PF01048	0.633
MOD_GlcNHglycan	384	387	PF01048	0.534
MOD_GlcNHglycan	388	391	PF01048	0.601
MOD_GlcNHglycan	419	422	PF01048	0.685
MOD_GlcNHglycan	45	48	PF01048	0.781
MOD_GlcNHglycan	533	536	PF01048	0.511
MOD_GlcNHglycan	542	545	PF01048	0.481
MOD_GlcNHglycan	57	60	PF01048	0.555
MOD_GlcNHglycan	772	775	PF01048	0.424
MOD_GlcNHglycan	848	851	PF01048	0.378
MOD_GlcNHglycan	93	97	PF01048	0.397
MOD_GSK3_1	231	238	PF00069	0.692
MOD_GSK3_1	24	31	PF00069	0.488
MOD_GSK3_1	248	255	PF00069	0.472
MOD_GSK3_1	314	321	PF00069	0.361
MOD_GSK3_1	382	389	PF00069	0.685
MOD_GSK3_1	394	401	PF00069	0.520
MOD_GSK3_1	429	436	PF00069	0.557
MOD_GSK3_1	45	52	PF00069	0.682
MOD_GSK3_1	515	522	PF00069	0.710
MOD_GSK3_1	550	557	PF00069	0.670
MOD_GSK3_1	601	608	PF00069	0.503
MOD_GSK3_1	75	82	PF00069	0.525
MOD_GSK3_1	814	821	PF00069	0.408
MOD_N-GLC_1	118	123	PF02516	0.600
MOD_NEK2_1	100	105	PF00069	0.461
MOD_NEK2_1	166	171	PF00069	0.574
MOD_NEK2_1	22	27	PF00069	0.479
MOD_NEK2_1	349	354	PF00069	0.480
MOD_NEK2_1	40	45	PF00069	0.642
MOD_NEK2_1	706	711	PF00069	0.490
MOD_NEK2_1	772	777	PF00069	0.420
MOD_NEK2_1	8	13	PF00069	0.682
MOD_NEK2_1	92	97	PF00069	0.503
MOD_NEK2_2	80	85	PF00069	0.430
MOD_OFUCOSY	527	532	PF10250	0.634
MOD_PIKK_1	167	173	PF00454	0.566
MOD_PIKK_1	492	498	PF00454	0.608
MOD_PIKK_1	557	563	PF00454	0.462
MOD_PIKK_1	691	697	PF00454	0.514
MOD_PIKK_1	730	736	PF00454	0.667
MOD_PIKK_1	795	801	PF00454	0.478
MOD_PK_1	328	334	PF00069	0.425
MOD_PK_1	73	79	PF00069	0.591
MOD_PKA_2	339	345	PF00069	0.501
MOD_PKA_2	422	428	PF00069	0.597
MOD_PKA_2	500	506	PF00069	0.437
MOD_PKA_2	620	626	PF00069	0.541
MOD_PKA_2	655	661	PF00069	0.395
MOD_PKA_2	706	712	PF00069	0.453
MOD_PKA_2	777	783	PF00069	0.375
MOD_Plk_1	133	139	PF00069	0.466
MOD_Plk_1	16	22	PF00069	0.437
MOD_Plk_1	175	181	PF00069	0.517
MOD_Plk_1	567	573	PF00069	0.473
MOD_Plk_1	638	644	PF00069	0.493
MOD_Plk_2-3	157	163	PF00069	0.617
MOD_Plk_4	133	139	PF00069	0.466
MOD_Plk_4	169	175	PF00069	0.551
MOD_Plk_4	276	282	PF00069	0.443
MOD_Plk_4	349	355	PF00069	0.400
MOD_Plk_4	501	507	PF00069	0.448
MOD_Plk_4	567	573	PF00069	0.487
MOD_Plk_4	576	582	PF00069	0.348
MOD_Plk_4	772	778	PF00069	0.421
MOD_Plk_4	80	86	PF00069	0.532
MOD_ProDKin_1	318	324	PF00069	0.467
MOD_ProDKin_1	379	385	PF00069	0.678
MOD_ProDKin_1	388	394	PF00069	0.563
MOD_ProDKin_1	407	413	PF00069	0.635
MOD_ProDKin_1	487	493	PF00069	0.600
MOD_ProDKin_1	519	525	PF00069	0.760
MOD_ProDKin_1	741	747	PF00069	0.664
MOD_ProDKin_1	818	824	PF00069	0.398
MOD_SUMO_for_1	624	627	PF00179	0.579
MOD_SUMO_rev_2	331	339	PF00179	0.305
MOD_SUMO_rev_2	844	849	PF00179	0.393
TRG_DiLeu_BaEn_1	345	350	PF01217	0.352
TRG_DiLeu_BaLyEn_6	455	460	PF01217	0.368
TRG_DiLeu_BaLyEn_6	611	616	PF01217	0.483
TRG_ENDOCYTIC_2	23	26	PF00928	0.693
TRG_ENDOCYTIC_2	309	312	PF00928	0.334
TRG_ENDOCYTIC_2	577	580	PF00928	0.349
TRG_ENDOCYTIC_2	648	651	PF00928	0.382
TRG_ENDOCYTIC_2	85	88	PF00928	0.403
TRG_ER_diArg_1	2	5	PF00400	0.466
TRG_ER_diArg_1	440	442	PF00400	0.530
TRG_ER_diArg_1	613	615	PF00400	0.470
TRG_ER_diArg_1	620	622	PF00400	0.523
TRG_ER_diArg_1	805	807	PF00400	0.306
TRG_NES_CRM1_1	324	336	PF08389	0.402
TRG_Pf-PMV_PEXEL_1	614	619	PF00026	0.514
TRG_Pf-PMV_PEXEL_1	809	813	PF00026	0.398
TRG_Pf-PMV_PEXEL_1	97	101	PF00026	0.571

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6M5	Leptomonas seymouri	38%	100%
A0A3S7WX62	Leishmania donovani	78%	98%
A4HCE3	Leishmania braziliensis	62%	99%
A4HZW6	Leishmania infantum	78%	98%
Q4QBQ5	Leishmania major	80%	100%