DNA primase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

DNA primase

Gene product:

DNA primase small subunit, putative

Species:

Leishmania mexicana

UniProt:

E9AVM3_LEIMU

TriTrypDb:

LmxM.22.0390

Length:

451

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0000428	DNA-directed RNA polymerase complex	4	12
GO:0030880	RNA polymerase complex	3	12
GO:0032991	protein-containing complex	1	12
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	12
GO:0140535	intracellular protein-containing complex	2	12
GO:1902494	catalytic complex	2	12
GO:1990234	transferase complex	3	12
GO:0005654	nucleoplasm	2	1
GO:0005658	alpha DNA polymerase:primase complex	4	1
GO:0005737	cytoplasm	2	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0042575	DNA polymerase complex	3	1
GO:0055029	nuclear DNA-directed RNA polymerase complex	3	1
GO:0097014	ciliary plasm	5	1
GO:0099568	cytoplasmic region	3	1
GO:0110165	cellular anatomical entity	1	1
GO:0140513	nuclear protein-containing complex	2	1

Expansion

Sequence features

E9AVM3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AVM3

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006269	DNA replication, synthesis of RNA primer	6	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009058	biosynthetic process	2	1
GO:0009059	macromolecule biosynthetic process	4	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0018130	heterocycle biosynthetic process	4	1
GO:0019438	aromatic compound biosynthetic process	4	1
GO:0032774	RNA biosynthetic process	5	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034654	nucleobase-containing compound biosynthetic process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044249	cellular biosynthetic process	3	1
GO:0044271	cellular nitrogen compound biosynthetic process	4	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1
GO:1901362	organic cyclic compound biosynthetic process	4	1
GO:1901576	organic substance biosynthetic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0003896	DNA primase activity	7	12
GO:0003899	DNA-directed 5'-3' RNA polymerase activity	6	12
GO:0005488	binding	1	12
GO:0016740	transferase activity	2	12
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	12
GO:0016779	nucleotidyltransferase activity	4	12
GO:0034062	5'-3' RNA polymerase activity	5	12
GO:0043167	ion binding	2	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0097747	RNA polymerase activity	4	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	292	296	PF00656	0.444
CLV_C14_Caspase3-7	404	408	PF00656	0.485
CLV_C14_Caspase3-7	55	59	PF00656	0.748
CLV_C14_Caspase3-7	86	90	PF00656	0.674
CLV_MEL_PAP_1	26	32	PF00089	0.277
CLV_NRD_NRD_1	102	104	PF00675	0.306
CLV_NRD_NRD_1	220	222	PF00675	0.297
CLV_NRD_NRD_1	230	232	PF00675	0.297
CLV_NRD_NRD_1	322	324	PF00675	0.297
CLV_NRD_NRD_1	34	36	PF00675	0.421
CLV_NRD_NRD_1	379	381	PF00675	0.313
CLV_PCSK_KEX2_1	102	104	PF00082	0.320
CLV_PCSK_KEX2_1	220	222	PF00082	0.297
CLV_PCSK_KEX2_1	230	232	PF00082	0.297
CLV_PCSK_KEX2_1	322	324	PF00082	0.313
CLV_PCSK_KEX2_1	34	36	PF00082	0.421
CLV_PCSK_KEX2_1	379	381	PF00082	0.313
CLV_PCSK_SKI1_1	211	215	PF00082	0.333
CLV_PCSK_SKI1_1	233	237	PF00082	0.332
CLV_PCSK_SKI1_1	240	244	PF00082	0.288
CLV_PCSK_SKI1_1	274	278	PF00082	0.351
CLV_PCSK_SKI1_1	283	287	PF00082	0.283
CLV_PCSK_SKI1_1	437	441	PF00082	0.444
CLV_PCSK_SKI1_1	61	65	PF00082	0.541
CLV_Separin_Metazoa	129	133	PF03568	0.470
DEG_APCC_DBOX_1	379	387	PF00400	0.297
DEG_Nend_UBRbox_3	1	3	PF02207	0.661
DEG_SPOP_SBC_1	290	294	PF00917	0.444
DOC_CDC14_PxL_1	366	374	PF14671	0.297
DOC_CYCLIN_RxL_1	159	169	PF00134	0.327
DOC_CYCLIN_yCln2_LP_2	384	390	PF00134	0.297
DOC_MAPK_gen_1	220	227	PF00069	0.297
DOC_MAPK_gen_1	379	386	PF00069	0.313
DOC_MAPK_HePTP_8	376	388	PF00069	0.313
DOC_MAPK_MEF2A_6	220	227	PF00069	0.297
DOC_MAPK_MEF2A_6	379	388	PF00069	0.297
DOC_MAPK_NFAT4_5	220	228	PF00069	0.297
DOC_MAPK_RevD_3	365	380	PF00069	0.313
DOC_PP1_RVXF_1	160	167	PF00149	0.323
DOC_PP2B_LxvP_1	384	387	PF13499	0.297
DOC_USP7_MATH_1	191	195	PF00917	0.382
DOC_USP7_MATH_1	291	295	PF00917	0.332
DOC_USP7_MATH_1	33	37	PF00917	0.709
DOC_USP7_MATH_1	349	353	PF00917	0.417
DOC_WD40_RPTOR_TOS_1	166	172	PF00400	0.297
LIG_14-3-3_CanoR_1	116	121	PF00244	0.349
LIG_14-3-3_CanoR_1	256	260	PF00244	0.152
LIG_14-3-3_CanoR_1	283	290	PF00244	0.371
LIG_14-3-3_CanoR_1	29	33	PF00244	0.614
LIG_14-3-3_CanoR_1	34	41	PF00244	0.686
LIG_Actin_WH2_1	240	258	PF00022	0.417
LIG_Actin_WH2_2	243	258	PF00022	0.417
LIG_BIR_III_4	72	76	PF00653	0.545
LIG_deltaCOP1_diTrp_1	402	409	PF00928	0.396
LIG_EH1_1	360	368	PF00400	0.417
LIG_FHA_1	21	27	PF00498	0.368
LIG_FHA_1	312	318	PF00498	0.411
LIG_FHA_1	355	361	PF00498	0.404
LIG_FHA_1	392	398	PF00498	0.297
LIG_FHA_1	430	436	PF00498	0.497
LIG_FHA_2	234	240	PF00498	0.382
LIG_FHA_2	277	283	PF00498	0.357
LIG_FHA_2	376	382	PF00498	0.297
LIG_LIR_Apic_2	428	434	PF02991	0.368
LIG_LIR_Gen_1	104	114	PF02991	0.348
LIG_LIR_Gen_1	11	21	PF02991	0.342
LIG_LIR_Gen_1	187	197	PF02991	0.356
LIG_LIR_Gen_1	282	291	PF02991	0.289
LIG_LIR_Gen_1	402	411	PF02991	0.393
LIG_LIR_Nem_3	11	16	PF02991	0.351
LIG_LIR_Nem_3	112	117	PF02991	0.381
LIG_LIR_Nem_3	171	176	PF02991	0.297
LIG_LIR_Nem_3	207	213	PF02991	0.297
LIG_LIR_Nem_3	23	28	PF02991	0.342
LIG_LIR_Nem_3	282	288	PF02991	0.313
LIG_LIR_Nem_3	342	346	PF02991	0.297
LIG_LIR_Nem_3	402	406	PF02991	0.387
LIG_LYPXL_S_1	368	372	PF13949	0.297
LIG_LYPXL_yS_3	369	372	PF13949	0.297
LIG_Pex14_2	285	289	PF04695	0.297
LIG_SH2_CRK	173	177	PF00017	0.297
LIG_SH2_GRB2like	173	176	PF00017	0.382
LIG_SH2_NCK_1	13	17	PF00017	0.463
LIG_SH2_PTP2	212	215	PF00017	0.331
LIG_SH2_SRC	13	16	PF00017	0.436
LIG_SH2_SRC	173	176	PF00017	0.382
LIG_SH2_SRC	346	349	PF00017	0.152
LIG_SH2_STAP1	13	17	PF00017	0.359
LIG_SH2_STAT5	188	191	PF00017	0.326
LIG_SH2_STAT5	212	215	PF00017	0.285
LIG_SH2_STAT5	246	249	PF00017	0.313
LIG_SH2_STAT5	308	311	PF00017	0.366
LIG_SH2_STAT5	346	349	PF00017	0.317
LIG_SH2_STAT5	99	102	PF00017	0.332
LIG_SH3_3	246	252	PF00018	0.297
LIG_SH3_3	27	33	PF00018	0.588
LIG_SUMO_SIM_par_1	17	23	PF11976	0.361
LIG_TRAF2_1	122	125	PF00917	0.481
LIG_TRAF2_1	352	355	PF00917	0.411
LIG_TYR_ITIM	244	249	PF00017	0.297
LIG_TYR_ITIM	367	372	PF00017	0.297
MOD_CK1_1	119	125	PF00069	0.353
MOD_CK1_1	178	184	PF00069	0.321
MOD_CK1_1	36	42	PF00069	0.693
MOD_CK2_1	119	125	PF00069	0.353
MOD_CK2_1	233	239	PF00069	0.382
MOD_CK2_1	349	355	PF00069	0.417
MOD_CK2_1	5	11	PF00069	0.410
MOD_Cter_Amidation	218	221	PF01082	0.297
MOD_Cter_Amidation	320	323	PF01082	0.417
MOD_GlcNHglycan	121	124	PF01048	0.367
MOD_GlcNHglycan	180	183	PF01048	0.309
MOD_GlcNHglycan	217	220	PF01048	0.297
MOD_GlcNHglycan	314	317	PF01048	0.315
MOD_GlcNHglycan	35	38	PF01048	0.607
MOD_GlcNHglycan	51	54	PF01048	0.649
MOD_GlcNHglycan	77	80	PF01048	0.689
MOD_GSK3_1	112	119	PF00069	0.363
MOD_GSK3_1	20	27	PF00069	0.380
MOD_GSK3_1	251	258	PF00069	0.351
MOD_GSK3_1	33	40	PF00069	0.543
MOD_GSK3_1	49	56	PF00069	0.550
MOD_N-GLC_2	184	186	PF02516	0.223
MOD_NEK2_1	105	110	PF00069	0.321
MOD_NEK2_1	255	260	PF00069	0.417
MOD_NEK2_1	289	294	PF00069	0.330
MOD_OFUCOSY	189	195	PF10250	0.354
MOD_PIKK_1	20	26	PF00454	0.394
MOD_PIKK_1	276	282	PF00454	0.417
MOD_PIKK_1	28	34	PF00454	0.540
MOD_PKA_1	322	328	PF00069	0.152
MOD_PKA_2	255	261	PF00069	0.417
MOD_PKA_2	28	34	PF00069	0.592
MOD_PKA_2	322	328	PF00069	0.292
MOD_PKA_2	429	435	PF00069	0.511
MOD_PKB_1	231	239	PF00069	0.382
MOD_Plk_1	5	11	PF00069	0.412
MOD_Plk_2-3	441	447	PF00069	0.547
MOD_Plk_4	199	205	PF00069	0.403
MOD_Plk_4	245	251	PF00069	0.304
MOD_Plk_4	255	261	PF00069	0.335
MOD_Plk_4	342	348	PF00069	0.416
MOD_SUMO_for_1	44	47	PF00179	0.745
MOD_SUMO_for_1	66	69	PF00179	0.655
MOD_SUMO_rev_2	83	93	PF00179	0.624
TRG_AP2beta_CARGO_1	112	121	PF09066	0.482
TRG_DiLeu_BaEn_1	239	244	PF01217	0.331
TRG_DiLeu_BaLyEn_6	264	269	PF01217	0.405
TRG_ENDOCYTIC_2	13	16	PF00928	0.351
TRG_ENDOCYTIC_2	173	176	PF00928	0.297
TRG_ENDOCYTIC_2	246	249	PF00928	0.297
TRG_ENDOCYTIC_2	369	372	PF00928	0.297
TRG_ER_diArg_1	230	233	PF00400	0.417
TRG_ER_diArg_1	33	35	PF00400	0.415
TRG_Pf-PMV_PEXEL_1	162	167	PF00026	0.316
TRG_Pf-PMV_PEXEL_1	437	441	PF00026	0.376

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PDK6	Leptomonas seymouri	75%	100%
A0A0S4IXR0	Bodo saltans	51%	100%
A0A1X0NUV3	Trypanosomatidae	58%	100%
A0A3R7KRU9	Trypanosoma rangeli	60%	100%
A0A3S7WWZ8	Leishmania donovani	94%	100%
A4HC88	Leishmania braziliensis	84%	100%
A4HZR7	Leishmania infantum	93%	100%
C9ZRJ5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	58%	100%
O14215	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	34%	99%
P10363	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	32%	100%
P20664	Mus musculus	35%	100%
P34471	Caenorhabditis elegans	32%	100%
P49642	Homo sapiens	34%	100%
Q24317	Drosophila melanogaster	33%	100%
Q25998	Plasmodium falciparum (isolate K1 / Thailand)	33%	100%
Q4QBV6	Leishmania major	94%	100%
Q7KQM1	Plasmodium falciparum (isolate 3D7)	33%	100%
V5AVR1	Trypanosoma cruzi	58%	100%