ATP-dependent DNA helicase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

PIF1 helicase-like protein, putative

Species:

Leishmania mexicana

UniProt:

E9AVA3_LEIMU

TriTrypDb:

LmxM.21.1190

Length:

776

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 15

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	13
GO:0110165	cellular anatomical entity	1	13
GO:0005634	nucleus	5	1
GO:0005657	replication fork	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1

Expansion

Sequence features

E9AVA3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AVA3

Function

Biological processes
Term	Name	Level	Count
GO:0000723	telomere maintenance	5	13
GO:0006139	nucleobase-containing compound metabolic process	3	13
GO:0006259	DNA metabolic process	4	13
GO:0006281	DNA repair	5	13
GO:0006310	DNA recombination	5	13
GO:0006725	cellular aromatic compound metabolic process	3	13
GO:0006807	nitrogen compound metabolic process	2	13
GO:0006950	response to stress	2	13
GO:0006974	DNA damage response	4	13
GO:0006996	organelle organization	4	13
GO:0008152	metabolic process	1	13
GO:0009987	cellular process	1	13
GO:0016043	cellular component organization	3	13
GO:0032200	telomere organization	6	13
GO:0033554	cellular response to stress	3	13
GO:0034641	cellular nitrogen compound metabolic process	3	13
GO:0043170	macromolecule metabolic process	3	13
GO:0044237	cellular metabolic process	2	13
GO:0044238	primary metabolic process	2	13
GO:0044260	obsolete cellular macromolecule metabolic process	3	13
GO:0046483	heterocycle metabolic process	3	13
GO:0050896	response to stimulus	1	13
GO:0051276	chromosome organization	5	13
GO:0051716	cellular response to stimulus	2	13
GO:0071704	organic substance metabolic process	2	13
GO:0071840	cellular component organization or biogenesis	2	13
GO:0090304	nucleic acid metabolic process	4	13
GO:1901360	organic cyclic compound metabolic process	3	13
GO:0006260	DNA replication	5	1
GO:0032392	DNA geometric change	7	1
GO:0032508	DNA duplex unwinding	8	1
GO:0071103	DNA conformation change	6	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	13
GO:0003678	DNA helicase activity	3	13
GO:0003824	catalytic activity	1	13
GO:0004386	helicase activity	2	13
GO:0005488	binding	1	13
GO:0005524	ATP binding	5	13
GO:0008094	ATP-dependent activity, acting on DNA	2	13
GO:0016462	pyrophosphatase activity	5	13
GO:0016787	hydrolase activity	2	13
GO:0016817	hydrolase activity, acting on acid anhydrides	3	13
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	13
GO:0016887	ATP hydrolysis activity	7	13
GO:0017076	purine nucleotide binding	4	13
GO:0017111	ribonucleoside triphosphate phosphatase activity	6	13
GO:0030554	adenyl nucleotide binding	5	13
GO:0032553	ribonucleotide binding	3	13
GO:0032555	purine ribonucleotide binding	4	13
GO:0032559	adenyl ribonucleotide binding	5	13
GO:0035639	purine ribonucleoside triphosphate binding	4	13
GO:0036094	small molecule binding	2	13
GO:0043167	ion binding	2	13
GO:0043168	anion binding	3	13
GO:0097159	organic cyclic compound binding	2	13
GO:0097367	carbohydrate derivative binding	2	13
GO:0140097	catalytic activity, acting on DNA	3	13
GO:0140640	catalytic activity, acting on a nucleic acid	2	13
GO:0140657	ATP-dependent activity	1	13
GO:1901265	nucleoside phosphate binding	3	13
GO:1901363	heterocyclic compound binding	2	13
GO:0000287	magnesium ion binding	5	1
GO:0043169	cation binding	3	1
GO:0046872	metal ion binding	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	164	168	PF00656	0.715
CLV_MEL_PAP_1	508	514	PF00089	0.557
CLV_NRD_NRD_1	413	415	PF00675	0.425
CLV_NRD_NRD_1	488	490	PF00675	0.438
CLV_NRD_NRD_1	505	507	PF00675	0.430
CLV_NRD_NRD_1	522	524	PF00675	0.398
CLV_NRD_NRD_1	639	641	PF00675	0.422
CLV_NRD_NRD_1	684	686	PF00675	0.458
CLV_PCSK_KEX2_1	413	415	PF00082	0.423
CLV_PCSK_KEX2_1	488	490	PF00082	0.399
CLV_PCSK_KEX2_1	522	524	PF00082	0.483
CLV_PCSK_KEX2_1	639	641	PF00082	0.422
CLV_PCSK_KEX2_1	769	771	PF00082	0.790
CLV_PCSK_KEX2_1	93	95	PF00082	0.569
CLV_PCSK_PC1ET2_1	769	771	PF00082	0.790
CLV_PCSK_PC1ET2_1	93	95	PF00082	0.616
CLV_PCSK_SKI1_1	299	303	PF00082	0.681
CLV_PCSK_SKI1_1	497	501	PF00082	0.547
CLV_PCSK_SKI1_1	664	668	PF00082	0.421
CLV_PCSK_SKI1_1	669	673	PF00082	0.423
CLV_PCSK_SKI1_1	769	773	PF00082	0.797
DEG_COP1_1	614	624	PF00400	0.262
DEG_SPOP_SBC_1	205	209	PF00917	0.554
DEG_SPOP_SBC_1	210	214	PF00917	0.602
DEG_SPOP_SBC_1	407	411	PF00917	0.313
DOC_CYCLIN_yCln2_LP_2	400	406	PF00134	0.313
DOC_MAPK_gen_1	639	646	PF00069	0.406
DOC_MAPK_gen_1	683	692	PF00069	0.414
DOC_MAPK_gen_1	93	100	PF00069	0.522
DOC_MAPK_MEF2A_6	349	358	PF00069	0.185
DOC_MAPK_MEF2A_6	639	646	PF00069	0.347
DOC_MAPK_MEF2A_6	654	662	PF00069	0.373
DOC_MAPK_MEF2A_6	685	694	PF00069	0.249
DOC_PP1_RVXF_1	248	254	PF00149	0.583
DOC_PP2B_LxvP_1	400	403	PF13499	0.313
DOC_PP2B_LxvP_1	404	407	PF13499	0.283
DOC_PP2B_LxvP_1	436	439	PF13499	0.301
DOC_SPAK_OSR1_1	511	515	PF12202	0.545
DOC_USP7_MATH_1	130	134	PF00917	0.822
DOC_USP7_MATH_1	200	204	PF00917	0.764
DOC_USP7_MATH_1	210	214	PF00917	0.576
DOC_USP7_MATH_1	219	223	PF00917	0.850
DOC_USP7_MATH_1	226	230	PF00917	0.774
DOC_USP7_MATH_1	239	243	PF00917	0.676
DOC_USP7_MATH_1	407	411	PF00917	0.357
DOC_USP7_MATH_1	560	564	PF00917	0.570
DOC_USP7_MATH_1	702	706	PF00917	0.463
DOC_USP7_MATH_1	735	739	PF00917	0.748
DOC_USP7_MATH_1	749	753	PF00917	0.787
DOC_USP7_UBL2_3	119	123	PF12436	0.550
DOC_WW_Pin1_4	206	211	PF00397	0.758
DOC_WW_Pin1_4	490	495	PF00397	0.362
DOC_WW_Pin1_4	730	735	PF00397	0.770
LIG_14-3-3_CanoR_1	221	225	PF00244	0.820
LIG_14-3-3_CanoR_1	322	331	PF00244	0.441
LIG_14-3-3_CanoR_1	413	423	PF00244	0.332
LIG_14-3-3_CanoR_1	544	553	PF00244	0.499
LIG_14-3-3_CanoR_1	612	620	PF00244	0.501
LIG_14-3-3_CanoR_1	634	638	PF00244	0.589
LIG_14-3-3_CanoR_1	763	769	PF00244	0.589
LIG_14-3-3_CanoR_1	84	88	PF00244	0.512
LIG_14-3-3_CanoR_1	94	99	PF00244	0.423
LIG_Actin_WH2_2	236	254	PF00022	0.461
LIG_Actin_WH2_2	555	573	PF00022	0.452
LIG_APCC_ABBA_1	22	27	PF00400	0.467
LIG_APCC_ABBAyCdc20_2	21	27	PF00400	0.475
LIG_BIR_II_1	1	5	PF00653	0.641
LIG_BRCT_BRCA1_1	386	390	PF00533	0.456
LIG_BRCT_BRCA1_1	538	542	PF00533	0.568
LIG_CaM_IQ_9	113	129	PF13499	0.602
LIG_FHA_1	101	107	PF00498	0.380
LIG_FHA_1	279	285	PF00498	0.461
LIG_FHA_1	318	324	PF00498	0.595
LIG_FHA_1	387	393	PF00498	0.313
LIG_FHA_1	491	497	PF00498	0.396
LIG_FHA_1	515	521	PF00498	0.387
LIG_FHA_1	567	573	PF00498	0.511
LIG_FHA_1	623	629	PF00498	0.581
LIG_FHA_1	753	759	PF00498	0.591
LIG_FHA_1	763	769	PF00498	0.578
LIG_FHA_1	84	90	PF00498	0.445
LIG_FHA_2	100	106	PF00498	0.455
LIG_FHA_2	613	619	PF00498	0.434
LIG_FHA_2	691	697	PF00498	0.425
LIG_LIR_Gen_1	387	397	PF02991	0.398
LIG_LIR_Gen_1	434	441	PF02991	0.301
LIG_LIR_Gen_1	547	558	PF02991	0.517
LIG_LIR_Nem_3	387	393	PF02991	0.365
LIG_LIR_Nem_3	434	438	PF02991	0.407
LIG_LIR_Nem_3	539	545	PF02991	0.444
LIG_LIR_Nem_3	547	553	PF02991	0.422
LIG_LIR_Nem_3	63	69	PF02991	0.440
LIG_LIR_Nem_3	710	714	PF02991	0.474
LIG_MAD2	398	406	PF02301	0.360
LIG_RPA_C_Fungi	758	770	PF08784	0.468
LIG_SH2_CRK	550	554	PF00017	0.503
LIG_SH2_NCK_1	550	554	PF00017	0.534
LIG_SH2_PTP2	678	681	PF00017	0.354
LIG_SH2_STAT3	529	532	PF00017	0.391
LIG_SH2_STAT5	678	681	PF00017	0.354
LIG_SH3_2	216	221	PF14604	0.629
LIG_SH3_3	213	219	PF00018	0.696
LIG_SH3_3	400	406	PF00018	0.317
LIG_SH3_3	5	11	PF00018	0.570
LIG_SH3_3	559	565	PF00018	0.469
LIG_SH3_3	580	586	PF00018	0.441
LIG_SH3_3	603	609	PF00018	0.518
LIG_SH3_3	84	90	PF00018	0.451
LIG_SUMO_SIM_anti_2	375	381	PF11976	0.360
LIG_SUMO_SIM_anti_2	691	696	PF11976	0.381
LIG_SUMO_SIM_par_1	375	381	PF11976	0.288
LIG_SUMO_SIM_par_1	428	434	PF11976	0.316
LIG_SUMO_SIM_par_1	575	581	PF11976	0.393
LIG_SUMO_SIM_par_1	693	698	PF11976	0.435
LIG_TRAF2_1	175	178	PF00917	0.596
LIG_TRAF2_1	445	448	PF00917	0.447
MOD_CDK_SPxxK_3	490	497	PF00069	0.360
MOD_CK1_1	214	220	PF00069	0.751
MOD_CK1_1	230	236	PF00069	0.717
MOD_CK1_1	383	389	PF00069	0.400
MOD_CK1_1	43	49	PF00069	0.420
MOD_CK1_1	563	569	PF00069	0.525
MOD_CK1_1	719	725	PF00069	0.780
MOD_CK1_1	752	758	PF00069	0.711
MOD_CK1_1	762	768	PF00069	0.639
MOD_CK2_1	171	177	PF00069	0.756
MOD_CK2_1	200	206	PF00069	0.815
MOD_CK2_1	612	618	PF00069	0.449
MOD_CK2_1	667	673	PF00069	0.479
MOD_Cter_Amidation	504	507	PF01082	0.531
MOD_GlcNHglycan	149	152	PF01048	0.700
MOD_GlcNHglycan	15	18	PF01048	0.466
MOD_GlcNHglycan	161	164	PF01048	0.755
MOD_GlcNHglycan	173	177	PF01048	0.674
MOD_GlcNHglycan	236	239	PF01048	0.708
MOD_GlcNHglycan	272	275	PF01048	0.404
MOD_GlcNHglycan	344	347	PF01048	0.355
MOD_GlcNHglycan	45	48	PF01048	0.522
MOD_GlcNHglycan	704	707	PF01048	0.541
MOD_GlcNHglycan	737	740	PF01048	0.829
MOD_GSK3_1	172	179	PF00069	0.700
MOD_GSK3_1	200	207	PF00069	0.692
MOD_GSK3_1	210	217	PF00069	0.627
MOD_GSK3_1	226	233	PF00069	0.747
MOD_GSK3_1	234	241	PF00069	0.652
MOD_GSK3_1	380	387	PF00069	0.465
MOD_GSK3_1	715	722	PF00069	0.648
MOD_GSK3_1	725	732	PF00069	0.489
MOD_N-GLC_1	130	135	PF02516	0.702
MOD_N-GLC_1	234	239	PF02516	0.605
MOD_NEK2_1	13	18	PF00069	0.502
MOD_NEK2_1	161	166	PF00069	0.637
MOD_NEK2_1	204	209	PF00069	0.719
MOD_NEK2_1	211	216	PF00069	0.626
MOD_NEK2_1	384	389	PF00069	0.360
MOD_NEK2_1	578	583	PF00069	0.447
MOD_NEK2_1	635	640	PF00069	0.509
MOD_NEK2_1	667	672	PF00069	0.502
MOD_NEK2_1	690	695	PF00069	0.499
MOD_NEK2_1	759	764	PF00069	0.503
MOD_NEK2_2	20	25	PF00069	0.423
MOD_NEK2_2	337	342	PF00069	0.301
MOD_NEK2_2	648	653	PF00069	0.362
MOD_NEK2_2	749	754	PF00069	0.536
MOD_PIKK_1	211	217	PF00454	0.781
MOD_PIKK_1	408	414	PF00454	0.357
MOD_PIKK_1	720	726	PF00454	0.765
MOD_PK_1	94	100	PF00069	0.556
MOD_PKA_2	147	153	PF00069	0.796
MOD_PKA_2	220	226	PF00069	0.707
MOD_PKA_2	415	421	PF00069	0.325
MOD_PKA_2	43	49	PF00069	0.444
MOD_PKA_2	54	60	PF00069	0.483
MOD_PKA_2	611	617	PF00069	0.559
MOD_PKA_2	633	639	PF00069	0.567
MOD_PKA_2	743	749	PF00069	0.732
MOD_PKA_2	762	768	PF00069	0.764
MOD_PKA_2	83	89	PF00069	0.469
MOD_Plk_1	380	386	PF00069	0.361
MOD_Plk_1	514	520	PF00069	0.518
MOD_Plk_1	560	566	PF00069	0.453
MOD_Plk_1	667	673	PF00069	0.518
MOD_Plk_1	688	694	PF00069	0.420
MOD_Plk_4	220	226	PF00069	0.818
MOD_Plk_4	515	521	PF00069	0.424
MOD_Plk_4	563	569	PF00069	0.495
MOD_Plk_4	62	68	PF00069	0.455
MOD_Plk_4	667	673	PF00069	0.526
MOD_Plk_4	690	696	PF00069	0.280
MOD_Plk_4	83	89	PF00069	0.434
MOD_Plk_4	94	100	PF00069	0.451
MOD_ProDKin_1	206	212	PF00069	0.759
MOD_ProDKin_1	490	496	PF00069	0.356
MOD_ProDKin_1	730	736	PF00069	0.771
MOD_SUMO_for_1	771	774	PF00179	0.649
TRG_DiLeu_BaEn_1	515	520	PF01217	0.545
TRG_DiLeu_BaLyEn_6	331	336	PF01217	0.441
TRG_ENDOCYTIC_2	550	553	PF00928	0.471
TRG_ENDOCYTIC_2	678	681	PF00928	0.354
TRG_ER_diArg_1	293	296	PF00400	0.601
TRG_ER_diArg_1	413	416	PF00400	0.412
TRG_ER_diArg_1	487	489	PF00400	0.404
TRG_NES_CRM1_1	656	668	PF08389	0.555
TRG_NLS_MonoExtC_3	768	774	PF00514	0.797
TRG_Pf-PMV_PEXEL_1	506	510	PF00026	0.453

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0S4IK87	Bodo saltans	30%	84%
A0A0S4JCS0	Bodo saltans	41%	100%
A0A1X0NZA2	Trypanosomatidae	38%	91%
A0A3Q8IBY0	Leishmania donovani	91%	99%
A0A3R7MKN4	Trypanosoma rangeli	29%	81%
A0A422NAK1	Trypanosoma rangeli	48%	99%
A4HBX5	Leishmania braziliensis	79%	99%
A4HZ95	Leishmania infantum	90%	99%
C9ZUJ0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	28%	78%
D0A177	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	97%
Q38CE9	Trypanosoma brucei brucei (strain 927/4 GUTat10.1)	44%	97%
Q4QC77	Leishmania major	90%	100%
Q57YG0	Trypanosoma brucei brucei (strain 927/4 GUTat10.1)	29%	78%
Q9UUA2	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	30%	96%
V5B8C2	Trypanosoma cruzi	30%	80%
V5BE68	Trypanosoma cruzi	47%	98%