Aspartate--tRNA ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

aspartyl-tRNA synthetase, putative

Species:

Leishmania mexicana

UniProt:

E9AV72_LEIMU

TriTrypDb:

LmxM.21.0895

Length:

641

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 4
Forrest at al. (procyclic)	no	yes: 4
Silverman et al.	no	yes: 2
Pissara et al.	yes	yes: 20

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 4

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 8

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 22
NetGPI	no	yes: 0, no: 22

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	23
GO:0110165	cellular anatomical entity	1	23
GO:0005829	cytosol	2	2
GO:0017101	aminoacyl-tRNA synthetase multienzyme complex	3	2
GO:0032991	protein-containing complex	1	2
GO:0140535	intracellular protein-containing complex	2	2
GO:1902494	catalytic complex	2	2

Expansion

Sequence features

E9AV72

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AV72

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	23
GO:0006139	nucleobase-containing compound metabolic process	3	23
GO:0006399	tRNA metabolic process	7	23
GO:0006418	tRNA aminoacylation for protein translation	6	23
GO:0006422	aspartyl-tRNA aminoacylation	7	23
GO:0006520	amino acid metabolic process	3	23
GO:0006725	cellular aromatic compound metabolic process	3	23
GO:0006807	nitrogen compound metabolic process	2	23
GO:0008152	metabolic process	1	23
GO:0009987	cellular process	1	23
GO:0016070	RNA metabolic process	5	23
GO:0019752	carboxylic acid metabolic process	5	23
GO:0034641	cellular nitrogen compound metabolic process	3	23
GO:0034660	ncRNA metabolic process	6	23
GO:0043038	amino acid activation	4	23
GO:0043039	tRNA aminoacylation	5	23
GO:0043170	macromolecule metabolic process	3	23
GO:0043436	oxoacid metabolic process	4	23
GO:0044237	cellular metabolic process	2	23
GO:0044238	primary metabolic process	2	23
GO:0044281	small molecule metabolic process	2	23
GO:0046483	heterocycle metabolic process	3	23
GO:0071704	organic substance metabolic process	2	23
GO:0090304	nucleic acid metabolic process	4	23
GO:1901360	organic cyclic compound metabolic process	3	23
GO:1901564	organonitrogen compound metabolic process	3	23

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	23
GO:0003676	nucleic acid binding	3	21
GO:0003824	catalytic activity	1	23
GO:0004812	aminoacyl-tRNA ligase activity	4	23
GO:0004815	aspartate-tRNA ligase activity	5	23
GO:0005488	binding	1	23
GO:0005524	ATP binding	5	23
GO:0016874	ligase activity	2	23
GO:0016875	ligase activity, forming carbon-oxygen bonds	3	23
GO:0017076	purine nucleotide binding	4	23
GO:0030554	adenyl nucleotide binding	5	23
GO:0032553	ribonucleotide binding	3	23
GO:0032555	purine ribonucleotide binding	4	23
GO:0032559	adenyl ribonucleotide binding	5	23
GO:0035639	purine ribonucleoside triphosphate binding	4	23
GO:0036094	small molecule binding	2	23
GO:0043167	ion binding	2	23
GO:0043168	anion binding	3	23
GO:0097159	organic cyclic compound binding	2	23
GO:0097367	carbohydrate derivative binding	2	23
GO:0140098	catalytic activity, acting on RNA	3	23
GO:0140101	catalytic activity, acting on a tRNA	4	23
GO:0140640	catalytic activity, acting on a nucleic acid	2	23
GO:1901265	nucleoside phosphate binding	3	23
GO:1901363	heterocyclic compound binding	2	23
GO:0003723	RNA binding	4	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	547	551	PF00656	0.463
CLV_C14_Caspase3-7	594	598	PF00656	0.503
CLV_NRD_NRD_1	15	17	PF00675	0.495
CLV_NRD_NRD_1	194	196	PF00675	0.242
CLV_NRD_NRD_1	301	303	PF00675	0.263
CLV_NRD_NRD_1	512	514	PF00675	0.321
CLV_PCSK_FUR_1	13	17	PF00082	0.434
CLV_PCSK_KEX2_1	15	17	PF00082	0.495
CLV_PCSK_KEX2_1	194	196	PF00082	0.242
CLV_PCSK_KEX2_1	39	41	PF00082	0.795
CLV_PCSK_KEX2_1	512	514	PF00082	0.321
CLV_PCSK_KEX2_1	637	639	PF00082	0.473
CLV_PCSK_PC1ET2_1	39	41	PF00082	0.768
CLV_PCSK_PC1ET2_1	637	639	PF00082	0.473
CLV_PCSK_SKI1_1	118	122	PF00082	0.523
CLV_PCSK_SKI1_1	151	155	PF00082	0.273
CLV_PCSK_SKI1_1	172	176	PF00082	0.376
CLV_PCSK_SKI1_1	179	183	PF00082	0.291
CLV_PCSK_SKI1_1	341	345	PF00082	0.331
CLV_PCSK_SKI1_1	615	619	PF00082	0.303
CLV_PCSK_SKI1_1	637	641	PF00082	0.342
CLV_Separin_Metazoa	413	417	PF03568	0.463
DEG_APCC_DBOX_1	139	147	PF00400	0.512
DEG_APCC_DBOX_1	148	156	PF00400	0.500
DEG_APCC_DBOX_1	614	622	PF00400	0.494
DEG_Nend_Nbox_1	1	3	PF02207	0.651
DEG_SPOP_SBC_1	200	204	PF00917	0.515
DEG_SPOP_SBC_1	30	34	PF00917	0.720
DOC_ANK_TNKS_1	464	471	PF00023	0.463
DOC_CKS1_1	278	283	PF01111	0.489
DOC_CKS1_1	79	84	PF01111	0.565
DOC_MAPK_gen_1	145	154	PF00069	0.479
DOC_MAPK_gen_1	519	528	PF00069	0.503
DOC_MAPK_gen_1	585	592	PF00069	0.529
DOC_MAPK_MEF2A_6	519	528	PF00069	0.503
DOC_PP1_RVXF_1	149	155	PF00149	0.444
DOC_PP4_FxxP_1	506	509	PF00568	0.567
DOC_USP7_MATH_1	161	165	PF00917	0.493
DOC_USP7_MATH_1	199	203	PF00917	0.493
DOC_USP7_MATH_1	30	34	PF00917	0.676
DOC_USP7_MATH_1	334	338	PF00917	0.439
DOC_USP7_MATH_1	47	51	PF00917	0.516
DOC_USP7_MATH_1	481	485	PF00917	0.570
DOC_USP7_MATH_1	552	556	PF00917	0.528
DOC_USP7_MATH_1	71	75	PF00917	0.746
DOC_USP7_UBL2_3	172	176	PF12436	0.576
DOC_USP7_UBL2_3	585	589	PF12436	0.442
DOC_WW_Pin1_4	179	184	PF00397	0.480
DOC_WW_Pin1_4	195	200	PF00397	0.536
DOC_WW_Pin1_4	220	225	PF00397	0.433
DOC_WW_Pin1_4	277	282	PF00397	0.478
DOC_WW_Pin1_4	306	311	PF00397	0.487
DOC_WW_Pin1_4	31	36	PF00397	0.624
DOC_WW_Pin1_4	443	448	PF00397	0.561
DOC_WW_Pin1_4	67	72	PF00397	0.781
DOC_WW_Pin1_4	78	83	PF00397	0.541
LIG_14-3-3_CanoR_1	104	114	PF00244	0.621
LIG_14-3-3_CanoR_1	257	266	PF00244	0.513
LIG_14-3-3_CanoR_1	3	7	PF00244	0.726
LIG_14-3-3_CanoR_1	341	349	PF00244	0.478
LIG_14-3-3_CanoR_1	40	45	PF00244	0.740
LIG_14-3-3_CanoR_1	435	440	PF00244	0.555
LIG_14-3-3_CanoR_1	477	481	PF00244	0.544
LIG_14-3-3_CanoR_1	48	54	PF00244	0.694
LIG_14-3-3_CanoR_1	537	543	PF00244	0.460
LIG_14-3-3_CanoR_1	581	587	PF00244	0.478
LIG_14-3-3_CanoR_1	627	632	PF00244	0.478
LIG_BRCT_BRCA1_1	291	295	PF00533	0.489
LIG_BRCT_BRCA1_1	73	77	PF00533	0.485
LIG_FHA_1	188	194	PF00498	0.541
LIG_FHA_1	253	259	PF00498	0.513
LIG_FHA_1	278	284	PF00498	0.535
LIG_FHA_1	342	348	PF00498	0.482
LIG_FHA_1	358	364	PF00498	0.461
LIG_FHA_1	498	504	PF00498	0.514
LIG_FHA_1	539	545	PF00498	0.553
LIG_FHA_2	408	414	PF00498	0.553
LIG_LIR_Apic_2	504	509	PF02991	0.567
LIG_LIR_Gen_1	274	283	PF02991	0.488
LIG_LIR_Nem_3	274	278	PF02991	0.495
LIG_LIR_Nem_3	344	349	PF02991	0.478
LIG_LIR_Nem_3	553	559	PF02991	0.488
LIG_PCNA_PIPBox_1	370	379	PF02747	0.442
LIG_SH2_CRK	540	544	PF00017	0.449
LIG_SH2_SRC	595	598	PF00017	0.489
LIG_SH2_STAP1	556	560	PF00017	0.521
LIG_SH2_STAT3	305	308	PF00017	0.500
LIG_SH2_STAT5	299	302	PF00017	0.499
LIG_SH2_STAT5	305	308	PF00017	0.499
LIG_SH2_STAT5	527	530	PF00017	0.489
LIG_SH2_STAT5	540	543	PF00017	0.487
LIG_SH2_STAT5	559	562	PF00017	0.530
LIG_SH2_STAT5	595	598	PF00017	0.489
LIG_SH3_1	540	546	PF00018	0.463
LIG_SH3_2	35	40	PF14604	0.469
LIG_SH3_2	549	554	PF14604	0.550
LIG_SH3_2	633	638	PF14604	0.543
LIG_SH3_3	14	20	PF00018	0.651
LIG_SH3_3	275	281	PF00018	0.544
LIG_SH3_3	32	38	PF00018	0.712
LIG_SH3_3	362	368	PF00018	0.581
LIG_SH3_3	540	546	PF00018	0.546
LIG_SH3_3	630	636	PF00018	0.483
LIG_SH3_3	76	82	PF00018	0.753
LIG_SUMO_SIM_anti_2	360	367	PF11976	0.504
LIG_SUMO_SIM_par_1	184	190	PF11976	0.503
LIG_SUMO_SIM_par_1	27	34	PF11976	0.698
LIG_SUMO_SIM_par_1	360	367	PF11976	0.508
LIG_UBA3_1	384	390	PF00899	0.382
LIG_WRC_WIRS_1	596	601	PF05994	0.377
LIG_WW_3	413	417	PF00397	0.299
MOD_CDK_SPK_2	277	282	PF00069	0.320
MOD_CDK_SPxK_1	448	454	PF00069	0.299
MOD_CDK_SPxxK_3	443	450	PF00069	0.270
MOD_CK1_1	113	119	PF00069	0.559
MOD_CK1_1	33	39	PF00069	0.678
MOD_CK1_1	446	452	PF00069	0.467
MOD_CK1_1	5	11	PF00069	0.538
MOD_CK1_1	64	70	PF00069	0.679
MOD_CK1_1	80	86	PF00069	0.761
MOD_CK2_1	121	127	PF00069	0.481
MOD_CK2_1	355	361	PF00069	0.340
MOD_CK2_1	407	413	PF00069	0.296
MOD_DYRK1A_RPxSP_1	195	199	PF00069	0.410
MOD_GlcNHglycan	127	130	PF01048	0.669
MOD_GlcNHglycan	259	262	PF01048	0.411
MOD_GlcNHglycan	455	458	PF01048	0.437
MOD_GlcNHglycan	469	473	PF01048	0.330
MOD_GlcNHglycan	49	52	PF01048	0.691
MOD_GlcNHglycan	566	569	PF01048	0.320
MOD_GlcNHglycan	57	60	PF01048	0.690
MOD_GlcNHglycan	582	585	PF01048	0.320
MOD_GlcNHglycan	63	66	PF01048	0.663
MOD_GlcNHglycan	73	76	PF01048	0.628
MOD_GlcNHglycan	82	85	PF01048	0.772
MOD_GlcNHglycan	97	100	PF01048	0.620
MOD_GSK3_1	121	128	PF00069	0.559
MOD_GSK3_1	195	202	PF00069	0.412
MOD_GSK3_1	216	223	PF00069	0.486
MOD_GSK3_1	253	260	PF00069	0.395
MOD_GSK3_1	285	292	PF00069	0.324
MOD_GSK3_1	29	36	PF00069	0.622
MOD_GSK3_1	435	442	PF00069	0.441
MOD_GSK3_1	53	60	PF00069	0.695
MOD_GSK3_1	63	70	PF00069	0.695
MOD_GSK3_1	73	80	PF00069	0.714
MOD_GSK3_1	84	91	PF00069	0.755
MOD_N-GLC_1	481	486	PF02516	0.438
MOD_NEK2_1	121	126	PF00069	0.536
MOD_NEK2_1	2	7	PF00069	0.653
MOD_NEK2_1	210	215	PF00069	0.333
MOD_NEK2_1	252	257	PF00069	0.394
MOD_NEK2_1	262	267	PF00069	0.421
MOD_NEK2_1	453	458	PF00069	0.262
MOD_NEK2_1	476	481	PF00069	0.393
MOD_NEK2_1	54	59	PF00069	0.798
MOD_NEK2_2	595	600	PF00069	0.367
MOD_PIKK_1	216	222	PF00454	0.512
MOD_PIKK_1	375	381	PF00454	0.469
MOD_PIKK_1	481	487	PF00454	0.457
MOD_PIKK_1	59	65	PF00454	0.637
MOD_PKA_1	39	45	PF00069	0.569
MOD_PKA_2	2	8	PF00069	0.699
MOD_PKA_2	39	45	PF00069	0.571
MOD_PKA_2	47	53	PF00069	0.537
MOD_PKA_2	476	482	PF00069	0.405
MOD_PKA_2	580	586	PF00069	0.469
MOD_Plk_1	135	141	PF00069	0.366
MOD_Plk_1	334	340	PF00069	0.335
MOD_Plk_1	481	487	PF00069	0.270
MOD_Plk_1	504	510	PF00069	0.365
MOD_Plk_2-3	588	594	PF00069	0.442
MOD_Plk_4	182	188	PF00069	0.337
MOD_Plk_4	627	633	PF00069	0.335
MOD_ProDKin_1	179	185	PF00069	0.323
MOD_ProDKin_1	195	201	PF00069	0.402
MOD_ProDKin_1	220	226	PF00069	0.434
MOD_ProDKin_1	277	283	PF00069	0.320
MOD_ProDKin_1	306	312	PF00069	0.333
MOD_ProDKin_1	31	37	PF00069	0.626
MOD_ProDKin_1	443	449	PF00069	0.437
MOD_ProDKin_1	67	73	PF00069	0.783
MOD_ProDKin_1	78	84	PF00069	0.541
MOD_SUMO_rev_2	164	174	PF00179	0.299
MOD_SUMO_rev_2	298	304	PF00179	0.299
MOD_SUMO_rev_2	630	639	PF00179	0.355
TRG_ER_diArg_1	13	16	PF00400	0.489
TRG_ER_diArg_1	139	142	PF00400	0.321
TRG_ER_diArg_1	146	149	PF00400	0.322
TRG_ER_diArg_1	193	195	PF00400	0.270
TRG_ER_diArg_1	512	514	PF00400	0.380
TRG_NES_CRM1_1	315	327	PF08389	0.355
TRG_Pf-PMV_PEXEL_1	341	345	PF00026	0.320
TRG_Pf-PMV_PEXEL_1	501	505	PF00026	0.367
TRG_Pf-PMV_PEXEL_1	512	516	PF00026	0.349

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P7P9	Leptomonas seymouri	60%	98%
A0A0N1HZP3	Leptomonas seymouri	43%	100%
A0A0N1IMR3	Leptomonas seymouri	26%	72%
A0A0S4IKL4	Bodo saltans	45%	100%
A0A0S4INT6	Bodo saltans	34%	100%
A0A0S4ISB0	Bodo saltans	49%	100%
A0A0S4JDH2	Bodo saltans	26%	76%
A0A1X0NXR0	Trypanosomatidae	53%	100%
A0A1X0P268	Trypanosomatidae	43%	100%
A0A1X0P9Y4	Trypanosomatidae	25%	86%
A0A3Q8IHQ2	Leishmania donovani	44%	100%
A0A3R7KLC9	Trypanosoma rangeli	54%	100%
A0A3R7LNW3	Trypanosoma rangeli	44%	100%
A0A3R7P1H1	Trypanosoma rangeli	25%	85%
A0A3S7WWM6	Leishmania donovani	90%	99%
A0A3S7X7X7	Leishmania donovani	25%	72%
A0PZX0	Clostridium novyi (strain NT)	22%	100%
A1JRL1	Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)	24%	100%
A2SPG1	Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z)	31%	100%
A4HBV0	Leishmania braziliensis	79%	100%
A4HHZ7	Leishmania braziliensis	44%	100%
A4I574	Leishmania infantum	44%	100%
A4IA13	Leishmania infantum	25%	72%
A4TJK5	Yersinia pestis (strain Pestoides F)	24%	100%
A5VMP9	Limosilactobacillus reuteri (strain DSM 20016)	25%	100%
A6LBU6	Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / CIP 104284 / JCM 5825 / NCTC 11152)	24%	100%
A6LNG3	Thermosipho melanesiensis (strain DSM 12029 / CIP 104789 / BI429)	24%	100%
A6LTP1	Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052)	24%	100%
A6TB35	Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)	23%	100%
A7FID0	Yersinia pseudotuberculosis serotype O:1b (strain IP 31758)	24%	100%
A7HME4	Fervidobacterium nodosum (strain ATCC 35602 / DSM 5306 / Rt17-B1)	25%	100%
A8ESW7	Aliarcobacter butzleri (strain RM4018)	22%	100%
A8F7R7	Pseudothermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / NBRC 107922 / TMO)	24%	100%
A9KIA6	Lachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg)	25%	100%
A9QYX8	Yersinia pestis bv. Antiqua (strain Angola)	24%	100%
B0B8B6	Chlamydia trachomatis serovar L2 (strain 434/Bu / ATCC VR-902B)	26%	100%
B0B9Z5	Chlamydia trachomatis serovar L2b (strain UCH-1/proctitis)	26%	100%
B1HV71	Lysinibacillus sphaericus (strain C3-41)	25%	100%
B1JLL5	Yersinia pseudotuberculosis serotype O:3 (strain YPIII)	24%	100%
B2GA02	Limosilactobacillus reuteri (strain JCM 1112)	25%	100%
B2J116	Nostoc punctiforme (strain ATCC 29133 / PCC 73102)	26%	100%
B2K319	Yersinia pseudotuberculosis serotype IB (strain PB1/+)	24%	100%
B2RHE0	Porphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / CIP 103683 / JCM 12257 / NCTC 11834 / 2561)	25%	100%
B2S3J9	Treponema pallidum subsp. pallidum (strain SS14)	26%	100%
B2TN05	Clostridium botulinum (strain Eklund 17B / Type B)	24%	100%
B2V351	Clostridium botulinum (strain Alaska E43 / Type E3)	24%	100%
B6JLK1	Helicobacter pylori (strain P12)	23%	100%
B7IE30	Thermosipho africanus (strain TCF52B)	24%	100%
C4ZI12	Agathobacter rectalis (strain ATCC 33656 / DSM 3377 / JCM 17463 / KCTC 5835 / VPI 0990)	25%	100%
C9ZMM2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	25%	86%
C9ZQK0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	100%
D0A1C3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	51%	100%
E9AGV6	Leishmania infantum	92%	100%
E9B0H1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	44%	100%
E9B520	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	73%
O83618	Treponema pallidum (strain Nichols)	26%	100%
O84546	Chlamydia trachomatis (strain D/UW-3/Cx)	26%	100%
P54263	Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)	27%	100%
Q17XK9	Helicobacter acinonychis (strain Sheeba)	22%	100%
Q1C820	Yersinia pestis bv. Antiqua (strain Antiqua)	24%	100%
Q1CJH0	Yersinia pestis bv. Antiqua (strain Nepal516)	24%	100%
Q1CTQ7	Helicobacter pylori (strain HPAG1)	24%	100%
Q2SR42	Mycoplasma capricolum subsp. capricolum (strain California kid / ATCC 27343 / NCTC 10154)	22%	100%
Q3KLF5	Chlamydia trachomatis serovar A (strain ATCC VR-571B / DSM 19440 / HAR-13)	26%	100%
Q46175	Chlamydia caviae (strain ATCC VR-813 / DSM 19441 / 03DC25 / GPIC)	25%	100%
Q4FMP8	Pelagibacter ubique (strain HTCC1062)	22%	100%
Q4Q7R2	Leishmania major	43%	100%
Q4QCA9	Leishmania major	91%	100%
Q5L734	Chlamydia abortus (strain DSM 27085 / S26/3)	26%	100%
Q66AU4	Yersinia pseudotuberculosis serotype I (strain IP32953)	24%	100%
Q6F1A0	Mesoplasma florum (strain ATCC 33453 / NBRC 100688 / NCTC 11704 / L1)	23%	100%
Q6FEH6	Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)	24%	100%
Q6LUN2	Photobacterium profundum (strain SS9)	22%	100%
Q7MXM0	Porphyromonas gingivalis (strain ATCC BAA-308 / W83)	25%	100%
Q831X4	Enterococcus faecalis (strain ATCC 700802 / V583)	29%	100%
Q892B2	Clostridium tetani (strain Massachusetts / E88)	22%	100%
Q8RGJ4	Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / DSM 15643 / BCRC 10681 / CIP 101130 / JCM 8532 / KCTC 2640 / LMG 13131 / VPI 4355)	24%	100%
Q8ZEV0	Yersinia pestis	24%	100%
Q980V3	Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)	30%	100%
V5AS93	Trypanosoma cruzi	44%	100%
V5DGS5	Trypanosoma cruzi	26%	86%