PPR_long domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania mexicana

UniProt:

E9AUV9_LEIMU

TriTrypDb:

LmxM.20.1580

Length:

1018

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 1, no: 10
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AUV9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AUV9

Function

Biological processes
Term	Name	Level	Count
GO:0000966	RNA 5'-end processing	7	1
GO:0001682	tRNA 5'-leader removal	9	1
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006396	RNA processing	6	1
GO:0006399	tRNA metabolic process	7	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008033	tRNA processing	8	1
GO:0008152	metabolic process	1	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0034470	ncRNA processing	7	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034660	ncRNA metabolic process	6	1
GO:0043170	macromolecule metabolic process	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:0099116	tRNA 5'-end processing	8	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	1
GO:0004518	nuclease activity	4	1
GO:0004519	endonuclease activity	5	1
GO:0004521	RNA endonuclease activity	5	1
GO:0004526	ribonuclease P activity	6	1
GO:0004540	RNA nuclease activity	4	1
GO:0004549	tRNA-specific ribonuclease activity	5	1
GO:0016787	hydrolase activity	2	1
GO:0016788	hydrolase activity, acting on ester bonds	3	1
GO:0016891	RNA endonuclease activity, producing 5'-phosphomonoesters	6	1
GO:0016893	endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	6	1
GO:0140098	catalytic activity, acting on RNA	3	1
GO:0140101	catalytic activity, acting on a tRNA	4	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	700	704	PF00656	0.494
CLV_NRD_NRD_1	1003	1005	PF00675	0.558
CLV_NRD_NRD_1	1013	1015	PF00675	0.557
CLV_NRD_NRD_1	44	46	PF00675	0.470
CLV_NRD_NRD_1	472	474	PF00675	0.447
CLV_NRD_NRD_1	567	569	PF00675	0.396
CLV_NRD_NRD_1	626	628	PF00675	0.435
CLV_NRD_NRD_1	629	631	PF00675	0.456
CLV_NRD_NRD_1	640	642	PF00675	0.458
CLV_NRD_NRD_1	925	927	PF00675	0.524
CLV_NRD_NRD_1	975	977	PF00675	0.535
CLV_PCSK_FUR_1	1009	1013	PF00082	0.598
CLV_PCSK_FUR_1	627	631	PF00082	0.445
CLV_PCSK_KEX2_1	1011	1013	PF00082	0.565
CLV_PCSK_KEX2_1	44	46	PF00082	0.642
CLV_PCSK_KEX2_1	472	474	PF00082	0.447
CLV_PCSK_KEX2_1	567	569	PF00082	0.380
CLV_PCSK_KEX2_1	621	623	PF00082	0.433
CLV_PCSK_KEX2_1	625	627	PF00082	0.442
CLV_PCSK_KEX2_1	629	631	PF00082	0.456
CLV_PCSK_KEX2_1	975	977	PF00082	0.589
CLV_PCSK_PC1ET2_1	1011	1013	PF00082	0.553
CLV_PCSK_PC1ET2_1	621	623	PF00082	0.433
CLV_PCSK_PC1ET2_1	625	627	PF00082	0.442
CLV_PCSK_PC7_1	1009	1015	PF00082	0.605
CLV_PCSK_PC7_1	622	628	PF00082	0.429
CLV_PCSK_PC7_1	971	977	PF00082	0.636
CLV_PCSK_SKI1_1	1004	1008	PF00082	0.538
CLV_PCSK_SKI1_1	224	228	PF00082	0.360
CLV_PCSK_SKI1_1	268	272	PF00082	0.385
CLV_PCSK_SKI1_1	622	626	PF00082	0.430
CLV_Separin_Metazoa	279	283	PF03568	0.363
DEG_COP1_1	359	368	PF00400	0.519
DEG_Nend_Nbox_1	1	3	PF02207	0.656
DEG_ODPH_VHL_1	873	884	PF01847	0.535
DEG_SCF_FBW7_2	150	157	PF00400	0.677
DOC_CKS1_1	727	732	PF01111	0.617
DOC_MAPK_DCC_7	664	674	PF00069	0.491
DOC_MAPK_gen_1	413	421	PF00069	0.453
DOC_MAPK_gen_1	472	479	PF00069	0.343
DOC_MAPK_gen_1	567	575	PF00069	0.370
DOC_MAPK_gen_1	641	648	PF00069	0.541
DOC_MAPK_HePTP_8	469	481	PF00069	0.345
DOC_MAPK_MEF2A_6	166	175	PF00069	0.318
DOC_MAPK_MEF2A_6	415	423	PF00069	0.452
DOC_MAPK_MEF2A_6	472	481	PF00069	0.352
DOC_PP2B_LxvP_1	133	136	PF13499	0.681
DOC_PP2B_LxvP_1	672	675	PF13499	0.588
DOC_PP2B_LxvP_1	945	948	PF13499	0.503
DOC_PP4_FxxP_1	727	730	PF00568	0.584
DOC_USP7_MATH_1	7	11	PF00917	0.694
DOC_USP7_MATH_1	875	879	PF00917	0.457
DOC_USP7_MATH_1	883	887	PF00917	0.491
DOC_USP7_UBL2_3	243	247	PF12436	0.518
DOC_USP7_UBL2_3	621	625	PF12436	0.424
DOC_WW_Pin1_4	103	108	PF00397	0.465
DOC_WW_Pin1_4	11	16	PF00397	0.420
DOC_WW_Pin1_4	150	155	PF00397	0.767
DOC_WW_Pin1_4	268	273	PF00397	0.328
DOC_WW_Pin1_4	360	365	PF00397	0.403
DOC_WW_Pin1_4	650	655	PF00397	0.639
DOC_WW_Pin1_4	702	707	PF00397	0.485
DOC_WW_Pin1_4	726	731	PF00397	0.592
DOC_WW_Pin1_4	955	960	PF00397	0.582
DOC_WW_Pin1_4	977	982	PF00397	0.601
LIG_14-3-3_CanoR_1	102	107	PF00244	0.566
LIG_14-3-3_CanoR_1	116	122	PF00244	0.628
LIG_14-3-3_CanoR_1	124	134	PF00244	0.715
LIG_14-3-3_CanoR_1	166	174	PF00244	0.320
LIG_14-3-3_CanoR_1	418	424	PF00244	0.463
LIG_14-3-3_CanoR_1	567	571	PF00244	0.388
LIG_14-3-3_CanoR_1	604	608	PF00244	0.538
LIG_14-3-3_CterR_2	1013	1018	PF00244	0.647
LIG_APCC_ABBA_1	507	512	PF00400	0.484
LIG_APCC_ABBAyCdc20_2	693	699	PF00400	0.490
LIG_BRCT_BRCA1_1	859	863	PF00533	0.603
LIG_deltaCOP1_diTrp_1	65	73	PF00928	0.453
LIG_deltaCOP1_diTrp_1	774	781	PF00928	0.530
LIG_FHA_1	118	124	PF00498	0.547
LIG_FHA_1	126	132	PF00498	0.530
LIG_FHA_1	269	275	PF00498	0.324
LIG_FHA_1	310	316	PF00498	0.326
LIG_FHA_1	387	393	PF00498	0.455
LIG_FHA_1	490	496	PF00498	0.481
LIG_FHA_1	502	508	PF00498	0.449
LIG_FHA_1	900	906	PF00498	0.660
LIG_FHA_2	151	157	PF00498	0.723
LIG_FHA_2	519	525	PF00498	0.409
LIG_FHA_2	727	733	PF00498	0.451
LIG_FHA_2	887	893	PF00498	0.656
LIG_LIR_Apic_2	51	55	PF02991	0.468
LIG_LIR_Apic_2	724	730	PF02991	0.581
LIG_LIR_Gen_1	137	147	PF02991	0.611
LIG_LIR_Gen_1	289	297	PF02991	0.475
LIG_LIR_Gen_1	389	398	PF02991	0.375
LIG_LIR_Gen_1	441	447	PF02991	0.473
LIG_LIR_Gen_1	535	542	PF02991	0.365
LIG_LIR_Gen_1	551	559	PF02991	0.360
LIG_LIR_Gen_1	569	578	PF02991	0.363
LIG_LIR_Gen_1	889	899	PF02991	0.538
LIG_LIR_Gen_1	928	934	PF02991	0.465
LIG_LIR_LC3C_4	204	209	PF02991	0.521
LIG_LIR_LC3C_4	468	471	PF02991	0.391
LIG_LIR_Nem_3	137	143	PF02991	0.585
LIG_LIR_Nem_3	240	245	PF02991	0.395
LIG_LIR_Nem_3	289	293	PF02991	0.336
LIG_LIR_Nem_3	341	347	PF02991	0.405
LIG_LIR_Nem_3	389	394	PF02991	0.377
LIG_LIR_Nem_3	462	467	PF02991	0.361
LIG_LIR_Nem_3	533	537	PF02991	0.365
LIG_LIR_Nem_3	569	573	PF02991	0.377
LIG_LIR_Nem_3	790	794	PF02991	0.428
LIG_LIR_Nem_3	833	837	PF02991	0.515
LIG_LIR_Nem_3	889	894	PF02991	0.534
LIG_LIR_Nem_3	928	933	PF02991	0.460
LIG_MYND_1	132	136	PF01753	0.640
LIG_NRP_CendR_1	1016	1018	PF00754	0.717
LIG_PCNA_yPIPBox_3	21	29	PF02747	0.567
LIG_Pex14_1	518	522	PF04695	0.384
LIG_Pex14_1	936	940	PF04695	0.443
LIG_Rb_LxCxE_1	367	380	PF01857	0.427
LIG_SH2_CRK	242	246	PF00017	0.392
LIG_SH2_CRK	290	294	PF00017	0.475
LIG_SH2_CRK	344	348	PF00017	0.391
LIG_SH2_CRK	353	357	PF00017	0.349
LIG_SH2_CRK	52	56	PF00017	0.468
LIG_SH2_CRK	791	795	PF00017	0.516
LIG_SH2_GRB2like	303	306	PF00017	0.328
LIG_SH2_GRB2like	391	394	PF00017	0.342
LIG_SH2_NCK_1	290	294	PF00017	0.410
LIG_SH2_NCK_1	303	307	PF00017	0.242
LIG_SH2_NCK_1	891	895	PF00017	0.550
LIG_SH2_PTP2	537	540	PF00017	0.351
LIG_SH2_SRC	303	306	PF00017	0.328
LIG_SH2_SRC	592	595	PF00017	0.402
LIG_SH2_SRC	842	845	PF00017	0.583
LIG_SH2_STAP1	16	20	PF00017	0.398
LIG_SH2_STAP1	290	294	PF00017	0.457
LIG_SH2_STAP1	353	357	PF00017	0.379
LIG_SH2_STAP1	453	457	PF00017	0.499
LIG_SH2_STAP1	467	471	PF00017	0.284
LIG_SH2_STAP1	534	538	PF00017	0.354
LIG_SH2_STAP1	570	574	PF00017	0.379
LIG_SH2_STAP1	69	73	PF00017	0.462
LIG_SH2_STAT3	69	72	PF00017	0.462
LIG_SH2_STAT5	177	180	PF00017	0.476
LIG_SH2_STAT5	391	394	PF00017	0.343
LIG_SH2_STAT5	537	540	PF00017	0.351
LIG_SH2_STAT5	592	595	PF00017	0.402
LIG_SH2_STAT5	638	641	PF00017	0.456
LIG_SH2_STAT5	932	935	PF00017	0.438
LIG_SH3_2	872	877	PF14604	0.506
LIG_SH3_3	101	107	PF00018	0.371
LIG_SH3_3	646	652	PF00018	0.683
LIG_SH3_3	665	671	PF00018	0.560
LIG_SH3_3	796	802	PF00018	0.323
LIG_SH3_3	869	875	PF00018	0.595
LIG_SH3_3	945	951	PF00018	0.511
LIG_SUMO_SIM_anti_2	190	195	PF11976	0.500
LIG_SUMO_SIM_anti_2	203	212	PF11976	0.357
LIG_SUMO_SIM_par_1	131	137	PF11976	0.709
LIG_SUMO_SIM_par_1	203	212	PF11976	0.408
LIG_SUMO_SIM_par_1	257	264	PF11976	0.264
LIG_SUMO_SIM_par_1	492	498	PF11976	0.498
LIG_TRAF2_1	154	157	PF00917	0.748
LIG_TRAF2_1	580	583	PF00917	0.399
LIG_TRAF2_1	634	637	PF00917	0.445
LIG_TRAF2_1	74	77	PF00917	0.473
LIG_TRAF2_1	878	881	PF00917	0.467
LIG_TRAF2_1	997	1000	PF00917	0.672
LIG_TRAF2_2	136	141	PF00917	0.698
LIG_TRFH_1	648	652	PF08558	0.694
LIG_TYR_ITSM	887	894	PF00017	0.662
LIG_UBA3_1	261	266	PF00899	0.344
LIG_UBA3_1	905	912	PF00899	0.525
LIG_WRC_WIRS_1	281	286	PF05994	0.385
LIG_WRC_WIRS_1	519	524	PF05994	0.257
MOD_CDK_SPK_2	268	273	PF00069	0.328
MOD_CDK_SPK_2	702	707	PF00069	0.485
MOD_CK1_1	106	112	PF00069	0.504
MOD_CK1_1	182	188	PF00069	0.431
MOD_CK1_1	321	327	PF00069	0.535
MOD_CK1_1	566	572	PF00069	0.383
MOD_CK1_1	823	829	PF00069	0.557
MOD_CK1_1	886	892	PF00069	0.622
MOD_CK1_1	965	971	PF00069	0.616
MOD_CK2_1	150	156	PF00069	0.727
MOD_CK2_1	31	37	PF00069	0.461
MOD_CK2_1	326	332	PF00069	0.454
MOD_CK2_1	518	524	PF00069	0.403
MOD_CK2_1	576	582	PF00069	0.379
MOD_CK2_1	689	695	PF00069	0.445
MOD_CK2_1	875	881	PF00069	0.453
MOD_CK2_1	886	892	PF00069	0.505
MOD_CK2_1	994	1000	PF00069	0.618
MOD_CMANNOS	683	686	PF00535	0.448
MOD_GlcNHglycan	113	116	PF01048	0.523
MOD_GlcNHglycan	150	153	PF01048	0.725
MOD_GlcNHglycan	211	214	PF01048	0.304
MOD_GlcNHglycan	289	293	PF01048	0.475
MOD_GlcNHglycan	33	36	PF01048	0.476
MOD_GlcNHglycan	396	399	PF01048	0.351
MOD_GlcNHglycan	579	582	PF01048	0.377
MOD_GlcNHglycan	724	727	PF01048	0.511
MOD_GlcNHglycan	768	771	PF01048	0.651
MOD_GlcNHglycan	821	825	PF01048	0.546
MOD_GlcNHglycan	859	862	PF01048	0.489
MOD_GSK3_1	102	109	PF00069	0.481
MOD_GSK3_1	111	118	PF00069	0.490
MOD_GSK3_1	185	192	PF00069	0.507
MOD_GSK3_1	318	325	PF00069	0.433
MOD_GSK3_1	326	333	PF00069	0.437
MOD_GSK3_1	356	363	PF00069	0.502
MOD_GSK3_1	489	496	PF00069	0.440
MOD_GSK3_1	7	14	PF00069	0.594
MOD_GSK3_1	722	729	PF00069	0.455
MOD_GSK3_1	918	925	PF00069	0.740
MOD_GSK3_1	962	969	PF00069	0.540
MOD_GSK3_1	995	1002	PF00069	0.650
MOD_N-GLC_1	148	153	PF02516	0.735
MOD_N-GLC_1	185	190	PF02516	0.446
MOD_N-GLC_1	255	260	PF02516	0.422
MOD_N-GLC_1	386	391	PF02516	0.460
MOD_N-GLC_2	461	463	PF02516	0.488
MOD_NEK2_1	1	6	PF00069	0.631
MOD_NEK2_1	1007	1012	PF00069	0.608
MOD_NEK2_1	246	251	PF00069	0.490
MOD_NEK2_1	280	285	PF00069	0.385
MOD_NEK2_1	330	335	PF00069	0.447
MOD_NEK2_1	356	361	PF00069	0.399
MOD_NEK2_1	394	399	PF00069	0.347
MOD_NEK2_1	424	429	PF00069	0.452
MOD_NEK2_1	576	581	PF00069	0.358
MOD_NEK2_1	752	757	PF00069	0.475
MOD_NEK2_1	966	971	PF00069	0.554
MOD_PIKK_1	134	140	PF00454	0.658
MOD_PIKK_1	165	171	PF00454	0.612
MOD_PIKK_1	322	328	PF00454	0.443
MOD_PIKK_1	358	364	PF00454	0.410
MOD_PIKK_1	918	924	PF00454	0.771
MOD_PIKK_1	980	986	PF00454	0.671
MOD_PK_1	189	195	PF00069	0.558
MOD_PKA_1	44	50	PF00069	0.503
MOD_PKA_1	687	693	PF00069	0.452
MOD_PKA_2	115	121	PF00069	0.622
MOD_PKA_2	165	171	PF00069	0.410
MOD_PKA_2	2	8	PF00069	0.689
MOD_PKA_2	44	50	PF00069	0.633
MOD_PKA_2	566	572	PF00069	0.391
MOD_PKA_2	603	609	PF00069	0.421
MOD_PKA_2	925	931	PF00069	0.487
MOD_PKA_2	995	1001	PF00069	0.586
MOD_Plk_1	189	195	PF00069	0.534
MOD_Plk_1	255	261	PF00069	0.416
MOD_Plk_1	288	294	PF00069	0.475
MOD_Plk_1	309	315	PF00069	0.328
MOD_Plk_1	322	328	PF00069	0.467
MOD_Plk_1	386	392	PF00069	0.452
MOD_Plk_1	60	66	PF00069	0.431
MOD_Plk_1	676	682	PF00069	0.572
MOD_Plk_1	886	892	PF00069	0.510
MOD_Plk_2-3	530	536	PF00069	0.381
MOD_Plk_4	189	195	PF00069	0.552
MOD_Plk_4	255	261	PF00069	0.561
MOD_Plk_4	387	393	PF00069	0.370
MOD_Plk_4	419	425	PF00069	0.480
MOD_Plk_4	603	609	PF00069	0.421
MOD_Plk_4	752	758	PF00069	0.472
MOD_Plk_4	886	892	PF00069	0.521
MOD_ProDKin_1	103	109	PF00069	0.467
MOD_ProDKin_1	11	17	PF00069	0.415
MOD_ProDKin_1	150	156	PF00069	0.769
MOD_ProDKin_1	268	274	PF00069	0.328
MOD_ProDKin_1	360	366	PF00069	0.410
MOD_ProDKin_1	650	656	PF00069	0.636
MOD_ProDKin_1	702	708	PF00069	0.485
MOD_ProDKin_1	726	732	PF00069	0.591
MOD_ProDKin_1	955	961	PF00069	0.570
MOD_ProDKin_1	977	983	PF00069	0.601
MOD_SUMO_for_1	706	709	PF00179	0.512
MOD_SUMO_for_1	787	790	PF00179	0.421
MOD_SUMO_rev_2	258	267	PF00179	0.363
MOD_SUMO_rev_2	441	450	PF00179	0.558
MOD_SUMO_rev_2	709	716	PF00179	0.554
MOD_SUMO_rev_2	790	800	PF00179	0.554
TRG_DiLeu_BaEn_2	570	576	PF01217	0.450
TRG_DiLeu_BaLyEn_6	433	438	PF01217	0.473
TRG_DiLeu_LyEn_5	129	134	PF01217	0.534
TRG_ENDOCYTIC_2	140	143	PF00928	0.544
TRG_ENDOCYTIC_2	242	245	PF00928	0.394
TRG_ENDOCYTIC_2	290	293	PF00928	0.328
TRG_ENDOCYTIC_2	303	306	PF00928	0.328
TRG_ENDOCYTIC_2	344	347	PF00928	0.404
TRG_ENDOCYTIC_2	353	356	PF00928	0.350
TRG_ENDOCYTIC_2	391	394	PF00928	0.342
TRG_ENDOCYTIC_2	442	445	PF00928	0.446
TRG_ENDOCYTIC_2	467	470	PF00928	0.363
TRG_ENDOCYTIC_2	537	540	PF00928	0.351
TRG_ENDOCYTIC_2	570	573	PF00928	0.365
TRG_ENDOCYTIC_2	791	794	PF00928	0.510
TRG_ENDOCYTIC_2	891	894	PF00928	0.640
TRG_ENDOCYTIC_2	930	933	PF00928	0.482
TRG_ER_diArg_1	1012	1014	PF00400	0.564
TRG_ER_diArg_1	1015	1018	PF00400	0.592
TRG_ER_diArg_1	368	371	PF00400	0.425
TRG_ER_diArg_1	43	45	PF00400	0.632
TRG_ER_diArg_1	471	473	PF00400	0.404
TRG_ER_diArg_1	626	629	PF00400	0.440
TRG_ER_diArg_1	974	976	PF00400	0.578
TRG_NES_CRM1_1	326	338	PF08389	0.535
TRG_NLS_Bipartite_1	609	629	PF00514	0.425
TRG_NLS_MonoCore_2	1010	1015	PF00514	0.595
TRG_NLS_MonoExtC_3	624	629	PF00514	0.431
TRG_NLS_MonoExtC_3	925	931	PF00514	0.569
TRG_NLS_MonoExtN_4	1009	1015	PF00514	0.576
TRG_NLS_MonoExtN_4	622	629	PF00514	0.432
TRG_NLS_MonoExtN_4	924	930	PF00514	0.624
TRG_Pf-PMV_PEXEL_1	318	323	PF00026	0.530
TRG_Pf-PMV_PEXEL_1	629	633	PF00026	0.454
TRG_Pf-PMV_PEXEL_1	903	907	PF00026	0.703

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HZS7	Leptomonas seymouri	82%	100%
A0A0S4JPI2	Bodo saltans	50%	94%
A0A1X0NWA5	Trypanosomatidae	67%	100%
A0A3Q8IBP6	Leishmania donovani	96%	100%
A0A422NTN1	Trypanosoma rangeli	66%	100%
A4HZ12	Leishmania infantum	96%	100%
C9ZIL7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	65%	99%
E9AIM6	Leishmania braziliensis	91%	100%
Q4QCM3	Leishmania major	96%	100%
V5B8D4	Trypanosoma cruzi	66%	98%