Putative axoneme central apparatus protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

paralyzed flagella protein 16

Species:

Leishmania mexicana

UniProt:

E9AUS9_LEIMU

TriTrypDb:

LmxM.20.1400

Length:

510

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 18

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005856	cytoskeleton	5	1
GO:0005930	axoneme	2	1
GO:0015630	microtubule cytoskeleton	6	1
GO:0043226	organelle	2	2
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	2
GO:0005929	cilium	4	1
GO:0031514	motile cilium	5	1
GO:0042995	cell projection	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0120025	plasma membrane bounded cell projection	3	1

Expansion

Sequence features

E9AUS9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AUS9

Function

Biological processes
Term	Name	Level	Count
GO:0000226	microtubule cytoskeleton organization	3	1
GO:0000281	mitotic cytokinesis	4	1
GO:0000910	cytokinesis	3	1
GO:0001539	cilium or flagellum-dependent cell motility	3	1
GO:0001578	microtubule bundle formation	4	1
GO:0003341	cilium movement	4	1
GO:0006996	organelle organization	4	1
GO:0007010	cytoskeleton organization	5	1
GO:0007017	microtubule-based process	2	1
GO:0007018	microtubule-based movement	3	1
GO:0009987	cellular process	1	1
GO:0016043	cellular component organization	3	1
GO:0022402	cell cycle process	2	1
GO:0022607	cellular component assembly	4	1
GO:0035082	axoneme assembly	5	1
GO:0048870	cell motility	2	1
GO:0060285	cilium-dependent cell motility	4	1
GO:0060294	cilium movement involved in cell motility	5	1
GO:0061640	cytoskeleton-dependent cytokinesis	4	1
GO:0071840	cellular component organization or biogenesis	2	1
GO:1903047	mitotic cell cycle process	3	1

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	1
GO:0005515	protein binding	2	1
GO:0008017	microtubule binding	5	1
GO:0008092	cytoskeletal protein binding	3	1
GO:0015631	tubulin binding	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_PCSK_KEX2_1	184	186	PF00082	0.253
CLV_PCSK_KEX2_1	226	228	PF00082	0.326
CLV_PCSK_KEX2_1	401	403	PF00082	0.358
CLV_PCSK_KEX2_1	448	450	PF00082	0.368
CLV_PCSK_PC1ET2_1	184	186	PF00082	0.253
CLV_PCSK_PC1ET2_1	226	228	PF00082	0.365
CLV_PCSK_PC1ET2_1	401	403	PF00082	0.334
CLV_PCSK_PC1ET2_1	448	450	PF00082	0.368
CLV_PCSK_SKI1_1	255	259	PF00082	0.312
CLV_PCSK_SKI1_1	4	8	PF00082	0.507
CLV_PCSK_SKI1_1	401	405	PF00082	0.322
CLV_PCSK_SKI1_1	47	51	PF00082	0.333
DOC_CYCLIN_RxL_1	44	53	PF00134	0.336
DOC_CYCLIN_yCln2_LP_2	45	51	PF00134	0.329
DOC_MAPK_DCC_7	166	174	PF00069	0.253
DOC_MAPK_MEF2A_6	166	174	PF00069	0.253
DOC_MAPK_RevD_3	170	185	PF00069	0.253
DOC_PP1_RVXF_1	15	21	PF00149	0.321
DOC_USP7_MATH_1	237	241	PF00917	0.333
DOC_USP7_MATH_1	390	394	PF00917	0.375
DOC_USP7_MATH_1	467	471	PF00917	0.438
DOC_USP7_MATH_1	69	73	PF00917	0.414
DOC_USP7_UBL2_3	15	19	PF12436	0.231
DOC_USP7_UBL2_3	401	405	PF12436	0.340
DOC_WW_Pin1_4	111	116	PF00397	0.359
DOC_WW_Pin1_4	195	200	PF00397	0.422
DOC_WW_Pin1_4	279	284	PF00397	0.319
DOC_WW_Pin1_4	386	391	PF00397	0.471
DOC_WW_Pin1_4	488	493	PF00397	0.362
LIG_14-3-3_CanoR_1	185	194	PF00244	0.268
LIG_14-3-3_CanoR_1	455	461	PF00244	0.325
LIG_14-3-3_CanoR_1	67	75	PF00244	0.411
LIG_Actin_WH2_2	169	186	PF00022	0.253
LIG_BIR_II_1	1	5	PF00653	0.437
LIG_BRCT_BRCA1_1	101	105	PF00533	0.306
LIG_FHA_1	280	286	PF00498	0.310
LIG_FHA_1	324	330	PF00498	0.302
LIG_FHA_1	84	90	PF00498	0.473
LIG_FHA_2	138	144	PF00498	0.268
LIG_FHA_2	188	194	PF00498	0.268
LIG_FHA_2	21	27	PF00498	0.298
LIG_FHA_2	495	501	PF00498	0.389
LIG_FHA_2	7	13	PF00498	0.428
LIG_LIR_Apic_2	385	390	PF02991	0.356
LIG_LIR_Apic_2	488	492	PF02991	0.501
LIG_LIR_LC3C_4	245	248	PF02991	0.295
LIG_LIR_Nem_3	251	256	PF02991	0.302
LIG_LIR_Nem_3	9	13	PF02991	0.494
LIG_MYND_1	415	419	PF01753	0.320
LIG_MYND_3	414	418	PF01753	0.306
LIG_Pex14_1	147	151	PF04695	0.293
LIG_Pex14_2	253	257	PF04695	0.295
LIG_PTB_Apo_2	476	483	PF02174	0.403
LIG_SH2_CRK	437	441	PF00017	0.445
LIG_SH2_SRC	413	416	PF00017	0.456
LIG_SH2_STAP1	437	441	PF00017	0.445
LIG_SH2_STAP1	494	498	PF00017	0.379
LIG_SH2_STAT3	505	508	PF00017	0.513
LIG_SH2_STAT5	151	154	PF00017	0.253
LIG_SH2_STAT5	301	304	PF00017	0.331
LIG_SH2_STAT5	319	322	PF00017	0.277
LIG_SH2_STAT5	387	390	PF00017	0.349
LIG_SH2_STAT5	413	416	PF00017	0.442
LIG_SH2_STAT5	432	435	PF00017	0.179
LIG_SH3_3	173	179	PF00018	0.253
LIG_SH3_3	409	415	PF00018	0.324
LIG_SH3_3	420	426	PF00018	0.312
LIG_SUMO_SIM_par_1	47	53	PF11976	0.337
LIG_TRAF2_1	71	74	PF00917	0.432
LIG_WRC_WIRS_1	7	12	PF05994	0.496
MOD_CK1_1	321	327	PF00069	0.378
MOD_CK1_1	389	395	PF00069	0.486
MOD_CK1_1	53	59	PF00069	0.422
MOD_CK2_1	137	143	PF00069	0.286
MOD_CK2_1	467	473	PF00069	0.433
MOD_CK2_1	494	500	PF00069	0.397
MOD_CK2_1	6	12	PF00069	0.497
MOD_CK2_1	68	74	PF00069	0.414
MOD_GlcNHglycan	101	104	PF01048	0.306
MOD_GlcNHglycan	143	146	PF01048	0.226
MOD_GlcNHglycan	320	323	PF01048	0.372
MOD_GlcNHglycan	366	369	PF01048	0.366
MOD_GlcNHglycan	59	62	PF01048	0.307
MOD_GlcNHglycan	80	83	PF01048	0.358
MOD_GSK3_1	137	144	PF00069	0.269
MOD_GSK3_1	300	307	PF00069	0.439
MOD_GSK3_1	386	393	PF00069	0.467
MOD_GSK3_1	467	474	PF00069	0.427
MOD_GSK3_1	53	60	PF00069	0.326
MOD_N-GLC_1	69	74	PF02516	0.403
MOD_N-GLC_2	479	481	PF02516	0.323
MOD_NEK2_1	20	25	PF00069	0.315
MOD_NEK2_1	201	206	PF00069	0.491
MOD_NEK2_1	323	328	PF00069	0.301
MOD_NEK2_1	471	476	PF00069	0.473
MOD_NEK2_1	50	55	PF00069	0.396
MOD_NEK2_1	88	93	PF00069	0.364
MOD_NEK2_2	179	184	PF00069	0.265
MOD_PIKK_1	121	127	PF00454	0.253
MOD_PIKK_1	83	89	PF00454	0.470
MOD_PKA_2	364	370	PF00069	0.327
MOD_Plk_1	179	185	PF00069	0.253
MOD_Plk_4	201	207	PF00069	0.447
MOD_Plk_4	494	500	PF00069	0.382
MOD_ProDKin_1	111	117	PF00069	0.287
MOD_ProDKin_1	195	201	PF00069	0.417
MOD_ProDKin_1	279	285	PF00069	0.314
MOD_ProDKin_1	386	392	PF00069	0.466
MOD_ProDKin_1	488	494	PF00069	0.359
MOD_SUMO_for_1	447	450	PF00179	0.355
MOD_SUMO_for_1	463	466	PF00179	0.373
MOD_SUMO_rev_2	220	225	PF00179	0.313
TRG_DiLeu_BaEn_1	242	247	PF01217	0.294
TRG_DiLeu_BaEn_1	284	289	PF01217	0.309
TRG_DiLeu_BaLyEn_6	213	218	PF01217	0.302
TRG_DiLeu_BaLyEn_6	379	384	PF01217	0.342
TRG_DiLeu_BaLyEn_6	416	421	PF01217	0.311
TRG_DiLeu_BaLyEn_6	45	50	PF01217	0.321
TRG_ENDOCYTIC_2	437	440	PF00928	0.450
TRG_Pf-PMV_PEXEL_1	4	8	PF00026	0.364
TRG_Pf-PMV_PEXEL_1	47	52	PF00026	0.329

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PE96	Leptomonas seymouri	95%	100%
A0A0S4J9K3	Bodo saltans	81%	100%
A0A1X0NXK0	Trypanosomatidae	85%	99%
A0A3Q8IEY0	Leishmania donovani	99%	100%
A0A3R7K990	Trypanosoma rangeli	86%	99%
A4HYY3	Leishmania infantum	99%	100%
B6HJ92	Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255)	24%	92%
C9ZIJ0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	83%	99%
E9AIJ7	Leishmania braziliensis	96%	100%
O14063	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	21%	94%
O35345	Mus musculus	21%	95%
O60684	Homo sapiens	21%	95%
O75602	Homo sapiens	62%	100%
P52293	Mus musculus	21%	96%
Q02821	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	22%	94%
Q4QCQ4	Leishmania major	98%	100%
Q5RBV0	Pongo abelii	21%	95%
Q71VM4	Oryza sativa subsp. japonica	23%	97%
Q9JLI7	Mus musculus	63%	100%
V5D560	Trypanosoma cruzi	86%	99%