Putative calpain-like cysteine peptidase

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

small myristoylated protein 1

Species:

Leishmania mexicana

UniProt:

E9AUS1_LEIMU

TriTrypDb:

LmxM.20.1310

Length:

131

Annotations

LeishMANIAdb interaction annotations

by homology
Contact email: avilan@ula.ve
Publication title: Plasminogen binding proteins in secreted membrane vesicles of Leishmania mexicana
Publication 1st author(s): Figuera
Publication Identifier(s): 23178693
Host organism: -1
Interaction detection method(s): enzymatic linked imunosorbent assay
Interaction type: direct interaction
Identification method participant A: identification by antibody
Identification method participant B: identification by antibody
ID(s) interactor A: E9AUS1
ID(s) interactor B: P00747
Taxid interactor A: Leishmania mexicana
Taxid interactor B: Homo sapiens
Biological role(s) interactor A: unspecified role
Biological role(s) interactor B: unspecified role
Experimental role(s) interactor A: bait

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 2
Forrest at al. (metacyclic)	no	yes: 6
Forrest at al. (procyclic)	no	yes: 8
Silverman et al.	no	yes: 1
Pissara et al.	yes	yes: 32

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 15

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 30

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 63
NetGPI	no	yes: 0, no: 63

Cellular components
Term	Name	Level	Count
GO:0005654	nucleoplasm	2	1
GO:0005737	cytoplasm	2	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0097014	ciliary plasm	5	1
GO:0097542	ciliary tip	2	1
GO:0099568	cytoplasmic region	3	1
GO:0110165	cellular anatomical entity	1	3
GO:0016020	membrane	2	2
GO:0020018	ciliary pocket membrane	6	1
GO:0031090	organelle membrane	3	2
GO:0031253	cell projection membrane	4	2
GO:0060170	ciliary membrane	5	2
GO:0098588	bounding membrane of organelle	4	2
GO:0098590	plasma membrane region	3	2

Expansion

Sequence features

E9AUS1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AUS1

PDB structure(s): 1r75_A , 2fe0_A

Function

Biological processes
Term	Name	Level	Count
GO:0001539	cilium or flagellum-dependent cell motility	3	1
GO:0009987	cellular process	1	2
GO:0048870	cell motility	2	1
GO:0060285	cilium-dependent cell motility	4	1
GO:0006996	organelle organization	4	1
GO:0016043	cellular component organization	3	1
GO:0022607	cellular component assembly	4	1
GO:0030030	cell projection organization	4	1
GO:0030031	cell projection assembly	5	1
GO:0044000	movement in host	4	1
GO:0044403	biological process involved in symbiotic interaction	2	1
GO:0044409	entry into host	5	1
GO:0044419	biological process involved in interspecies interaction between organisms	1	1
GO:0044782	cilium organization	5	1
GO:0051701	biological process involved in interaction with host	3	1
GO:0060271	cilium assembly	6	1
GO:0070925	organelle assembly	5	1
GO:0071840	cellular component organization or biogenesis	2	1
GO:0120031	plasma membrane bounded cell projection assembly	6	1
GO:0120036	plasma membrane bounded cell projection organization	5	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	1
GO:0016787	hydrolase activity	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	111	115	PF00656	0.452
CLV_NRD_NRD_1	42	44	PF00675	0.419
CLV_PCSK_FUR_1	43	47	PF00082	0.264
CLV_PCSK_KEX2_1	45	47	PF00082	0.362
CLV_PCSK_PC1ET2_1	45	47	PF00082	0.329
DOC_MAPK_gen_1	110	118	PF00069	0.316
DOC_USP7_UBL2_3	28	32	PF12436	0.443
LIG_BIR_III_2	114	118	PF00653	0.473
LIG_EH1_1	56	64	PF00400	0.250
LIG_eIF4E_1	57	63	PF01652	0.250
LIG_FHA_2	53	59	PF00498	0.331
LIG_FHA_2	62	68	PF00498	0.328
LIG_FHA_2	75	81	PF00498	0.338
LIG_LIR_LC3C_4	90	93	PF02991	0.302
LIG_SH2_GRB2like	14	17	PF00017	0.519
LIG_SH2_GRB2like	50	53	PF00017	0.299
LIG_SH2_SRC	14	17	PF00017	0.507
LIG_SH2_STAP1	57	61	PF00017	0.335
LIG_SH2_STAT5	14	17	PF00017	0.505
LIG_SH3_3	89	95	PF00018	0.335
LIG_UBA3_1	36	45	PF00899	0.319
LIG_WRC_WIRS_1	62	67	PF05994	0.250
MOD_CK2_1	52	58	PF00069	0.355
MOD_CK2_1	61	67	PF00069	0.381
MOD_CK2_1	74	80	PF00069	0.365
MOD_GSK3_1	4	11	PF00069	0.538
MOD_N-GLC_1	51	56	PF02516	0.384
MOD_N-GLC_1	8	13	PF02516	0.713
MOD_NEK2_1	74	79	PF00069	0.360
MOD_NMyristoyl	1	7	PF02799	0.625
MOD_PIKK_1	52	58	PF00454	0.461
MOD_Plk_1	52	58	PF00069	0.419
MOD_Plk_4	10	16	PF00069	0.476
MOD_Plk_4	17	23	PF00069	0.468
MOD_Plk_4	87	93	PF00069	0.357
MOD_SPalmitoyl_4	1	7	PF01529	0.573
MOD_SUMO_rev_2	67	74	PF00179	0.374
TRG_DiLeu_BaEn_1	58	63	PF01217	0.250
TRG_DiLeu_BaEn_2	96	102	PF01217	0.379
TRG_ER_diArg_1	122	125	PF00400	0.456
TRG_NLS_MonoCore_2	42	47	PF00514	0.420
TRG_NLS_MonoExtN_4	43	48	PF00514	0.412

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6Y3	Leptomonas seymouri	85%	99%
A0A0N0P7C3	Leptomonas seymouri	39%	90%
A0A0N1HYV4	Leptomonas seymouri	60%	100%
A0A0N1I5S6	Leptomonas seymouri	46%	85%
A0A0N1I8U4	Leptomonas seymouri	33%	97%
A0A0N1P9P7	Leptomonas seymouri	38%	67%
A0A0N1PCZ0	Leptomonas seymouri	45%	100%
A0A0N1PE11	Leptomonas seymouri	39%	92%
A0A1X0NNW3	Trypanosomatidae	50%	100%
A0A1X0NW79	Trypanosomatidae	44%	66%
A0A1X0NW95	Trypanosomatidae	77%	100%
A0A1X0NWH2	Trypanosomatidae	35%	94%
A0A1X0NWV3	Trypanosomatidae	75%	88%
A0A1X0NXS9	Trypanosomatidae	38%	96%
A0A1X0NXT9	Trypanosomatidae	39%	100%
A0A1X0NYV3	Trypanosomatidae	43%	95%
A0A3Q8I9V9	Leishmania donovani	48%	100%
A0A3Q8IBM6	Leishmania donovani	36%	98%
A0A3Q8IBP3	Leishmania donovani	38%	90%
A0A3Q8IDP8	Leishmania donovani	45%	87%
A0A3Q8IKN9	Leishmania donovani	95%	100%
A0A3R7MB04	Trypanosoma rangeli	45%	100%
A0A3R7MWC6	Trypanosoma rangeli	73%	98%
A0A3S7WW23	Leishmania donovani	59%	96%
A0A3S7WZ86	Leishmania donovani	40%	92%
A0A422MSY8	Trypanosoma rangeli	73%	78%
A0A422MSZ3	Trypanosoma rangeli	73%	100%
A0A422MUY0	Trypanosoma rangeli	42%	87%
A0A422N035	Trypanosoma rangeli	38%	94%
A0A422N058	Trypanosoma rangeli	40%	86%
A4H7N9	Leishmania braziliensis	46%	100%
A4H7P0	Leishmania braziliensis	46%	100%
A4HEG7	Leishmania braziliensis	39%	100%
A4HW29	Leishmania infantum	48%	100%
A4HYW5	Leishmania infantum	38%	90%
A4HYW6	Leishmania infantum	36%	98%
A4HYX1	Leishmania infantum	45%	87%
A4HYX3	Leishmania infantum	59%	96%
A4HYX4	Leishmania infantum	95%	100%
A4I1M3	Leishmania infantum	40%	92%
C9ZIF2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	38%	100%
C9ZIF5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	60%	100%
C9ZIF6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	61%	100%
E8NHG3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	78%
E9AIH7	Leishmania braziliensis	41%	90%
E9AIH8	Leishmania braziliensis	34%	96%
E9AII3	Leishmania braziliensis	45%	100%
E9AII5	Leishmania braziliensis	58%	100%
E9AII6	Leishmania braziliensis	92%	100%
E9APT0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	48%	100%
E9AUR2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	42%	90%
E9AUR3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	37%	100%
E9AUR8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	44%	100%
E9AUS0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	57%	100%
E9AXQ7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	40%	92%
P84150	Leishmania major	36%	100%
Q4Q9R4	Leishmania major	40%	100%
Q4QCS4	Leishmania major	38%	100%
Q4QFP8	Leishmania major	49%	100%
Q5SDH3	Leishmania major	42%	100%
Q5SDH4	Leishmania major	58%	100%
Q5SDH5	Leishmania major	93%	100%