Putative calpain-like cysteine peptidase

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

calpain-like cysteine peptidase, putative

Species:

Leishmania mexicana

UniProt:

E9AUS0_LEIMU

TriTrypDb:

LmxM.20.1300

Length:

136

Annotations

LeishMANIAdb interaction annotations

by homology
Contact email: avilan@ula.ve
Publication title: Plasminogen binding proteins in secreted membrane vesicles of Leishmania mexicana
Publication 1st author(s): Figuera
Publication Identifier(s): 23178693
Host organism: -1
Interaction detection method(s): enzymatic linked imunosorbent assay
Interaction type: direct interaction
Identification method participant A: identification by antibody
Identification method participant B: identification by antibody
ID(s) interactor A: E9AUS1
ID(s) interactor B: P00747
Taxid interactor A: Leishmania mexicana
Taxid interactor B: Homo sapiens
Biological role(s) interactor A: unspecified role
Biological role(s) interactor B: unspecified role
Experimental role(s) interactor A: bait

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 2
Forrest at al. (metacyclic)	no	yes: 5
Forrest at al. (procyclic)	no	yes: 7
Silverman et al.	no	yes: 1
Pissara et al.	yes	yes: 28

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 12

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 27

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 56
NetGPI	no	yes: 0, no: 56

Cellular components
Term	Name	Level	Count
GO:0005654	nucleoplasm	2	1
GO:0005737	cytoplasm	2	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0097014	ciliary plasm	5	1
GO:0097542	ciliary tip	2	1
GO:0099568	cytoplasmic region	3	1
GO:0110165	cellular anatomical entity	1	3
GO:0016020	membrane	2	2
GO:0020018	ciliary pocket membrane	6	1
GO:0031090	organelle membrane	3	2
GO:0031253	cell projection membrane	4	2
GO:0060170	ciliary membrane	5	2
GO:0098588	bounding membrane of organelle	4	2
GO:0098590	plasma membrane region	3	2

Expansion

Sequence features

E9AUS0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AUS0

PDB structure(s): 1r75_A , 2fe0_A

Function

Biological processes
Term	Name	Level	Count
GO:0001539	cilium or flagellum-dependent cell motility	3	1
GO:0009987	cellular process	1	2
GO:0048870	cell motility	2	1
GO:0060285	cilium-dependent cell motility	4	1
GO:0006996	organelle organization	4	1
GO:0016043	cellular component organization	3	1
GO:0022607	cellular component assembly	4	1
GO:0030030	cell projection organization	4	1
GO:0030031	cell projection assembly	5	1
GO:0044000	movement in host	4	1
GO:0044403	biological process involved in symbiotic interaction	2	1
GO:0044409	entry into host	5	1
GO:0044419	biological process involved in interspecies interaction between organisms	1	1
GO:0044782	cilium organization	5	1
GO:0051701	biological process involved in interaction with host	3	1
GO:0060271	cilium assembly	6	1
GO:0070925	organelle assembly	5	1
GO:0071840	cellular component organization or biogenesis	2	1
GO:0120031	plasma membrane bounded cell projection assembly	6	1
GO:0120036	plasma membrane bounded cell projection organization	5	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	1
GO:0016787	hydrolase activity	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	112	116	PF00656	0.440
CLV_NRD_NRD_1	41	43	PF00675	0.405
CLV_PCSK_KEX2_1	41	43	PF00082	0.367
CLV_PCSK_PC1ET2_1	41	43	PF00082	0.345
CLV_PCSK_SKI1_1	38	42	PF00082	0.408
CLV_PCSK_SKI1_1	79	83	PF00082	0.528
DEG_SCF_FBW7_1	92	99	PF00400	0.213
DOC_CKS1_1	93	98	PF01111	0.213
DOC_USP7_MATH_1	118	122	PF00917	0.576
DOC_USP7_UBL2_3	10	14	PF12436	0.540
DOC_USP7_UBL2_3	17	21	PF12436	0.351
DOC_WW_Pin1_4	92	97	PF00397	0.270
LIG_14-3-3_CanoR_1	61	67	PF00244	0.410
LIG_APCC_ABBAyCdc20_2	98	104	PF00400	0.462
LIG_BIR_III_2	115	119	PF00653	0.472
LIG_FHA_1	76	82	PF00498	0.361
LIG_FHA_1	89	95	PF00498	0.296
LIG_LYPXL_yS_3	117	120	PF13949	0.394
LIG_SH3_3	78	84	PF00018	0.255
LIG_SH3_3	90	96	PF00018	0.339
LIG_SH3_4	17	24	PF00018	0.339
LIG_SUMO_SIM_par_1	90	95	PF11976	0.331
LIG_UBA3_1	35	44	PF00899	0.307
MOD_CDK_SPxK_1	92	98	PF00069	0.213
MOD_CK1_1	121	127	PF00069	0.685
MOD_CK1_1	4	10	PF00069	0.647
MOD_GlcNHglycan	122	126	PF01048	0.615
MOD_GlcNHglycan	83	87	PF01048	0.358
MOD_GSK3_1	1	8	PF00069	0.574
MOD_GSK3_1	88	95	PF00069	0.457
MOD_N-GLC_1	4	9	PF02516	0.768
MOD_N-GLC_1	52	57	PF02516	0.375
MOD_N-GLC_1	62	67	PF02516	0.368
MOD_NEK2_1	1	6	PF00069	0.678
MOD_NEK2_1	75	80	PF00069	0.358
MOD_NMyristoyl	1	7	PF02799	0.635
MOD_PIKK_1	53	59	PF00454	0.449
MOD_Plk_1	53	59	PF00069	0.412
MOD_Plk_1	62	68	PF00069	0.398
MOD_Plk_1	70	76	PF00069	0.408
MOD_Plk_4	88	94	PF00069	0.367
MOD_ProDKin_1	92	98	PF00069	0.270
MOD_SUMO_rev_2	100	107	PF00179	0.487
TRG_DiLeu_BaLyEn_6	124	129	PF01217	0.443
TRG_ENDOCYTIC_2	117	120	PF00928	0.579
TRG_NLS_MonoCore_2	40	45	PF00514	0.411
TRG_NLS_MonoExtN_4	38	45	PF00514	0.248

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6Y3	Leptomonas seymouri	58%	100%
A0A0N1HYV4	Leptomonas seymouri	57%	100%
A0A0N1I5S6	Leptomonas seymouri	41%	88%
A0A0N1I8U4	Leptomonas seymouri	30%	100%
A0A0N1P9P7	Leptomonas seymouri	38%	70%
A0A0N1PCZ0	Leptomonas seymouri	48%	100%
A0A0N1PE11	Leptomonas seymouri	30%	96%
A0A1X0NNW3	Trypanosomatidae	47%	100%
A0A1X0NW79	Trypanosomatidae	44%	69%
A0A1X0NW95	Trypanosomatidae	62%	100%
A0A1X0NWV3	Trypanosomatidae	58%	91%
A0A1X0NXS9	Trypanosomatidae	37%	100%
A0A1X0NXT9	Trypanosomatidae	42%	100%
A0A1X0NYV3	Trypanosomatidae	35%	99%
A0A3Q8I9V9	Leishmania donovani	45%	100%
A0A3Q8IBM6	Leishmania donovani	30%	100%
A0A3Q8IDP8	Leishmania donovani	40%	90%
A0A3Q8IKN9	Leishmania donovani	57%	100%
A0A3R7MB04	Trypanosoma rangeli	46%	100%
A0A3R7MWC6	Trypanosoma rangeli	55%	100%
A0A3S7WW23	Leishmania donovani	90%	100%
A0A3S7WZ86	Leishmania donovani	30%	96%
A0A422MSY8	Trypanosoma rangeli	53%	81%
A0A422MSZ3	Trypanosoma rangeli	55%	100%
A0A422MUY0	Trypanosoma rangeli	42%	91%
A0A422N058	Trypanosoma rangeli	35%	89%
A4H7N9	Leishmania braziliensis	44%	100%
A4H7P0	Leishmania braziliensis	44%	100%
A4HEG7	Leishmania braziliensis	30%	100%
A4HW29	Leishmania infantum	45%	100%
A4HYW6	Leishmania infantum	30%	100%
A4HYX1	Leishmania infantum	40%	90%
A4HYX3	Leishmania infantum	90%	100%
A4HYX4	Leishmania infantum	57%	100%
A4I1M3	Leishmania infantum	30%	96%
C9ZIF2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	38%	100%
C9ZIF5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	51%	100%
C9ZIF6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	50%	100%
E9AIH7	Leishmania braziliensis	35%	100%
E9AIH8	Leishmania braziliensis	29%	100%
E9AII3	Leishmania braziliensis	40%	100%
E9AII5	Leishmania braziliensis	76%	100%
E9AII6	Leishmania braziliensis	57%	100%
E9APT0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	45%	100%
E9AUR2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
E9AUR3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	30%	99%
E9AUR8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	38%	100%
E9AUS1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	57%	100%
E9AXQ7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	30%	96%
P84150	Leishmania major	31%	100%
Q4Q9R4	Leishmania major	30%	100%
Q4QFP8	Leishmania major	45%	100%
Q5SDH3	Leishmania major	43%	89%
Q5SDH4	Leishmania major	90%	100%
Q5SDH5	Leishmania major	57%	100%