Putative calpain-like cysteine peptidase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

calpain-like cysteine peptidase, putative

Species:

Leishmania mexicana

UniProt:

E9AUQ8_LEIMU

TriTrypDb:

LmxM.20.1185

Length:

651

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 15

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 1, no: 26
NetGPI	no	yes: 0, no: 27

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AUQ8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AUQ8

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	28
GO:0006807	nitrogen compound metabolic process	2	28
GO:0008152	metabolic process	1	28
GO:0019538	protein metabolic process	3	28
GO:0043170	macromolecule metabolic process	3	28
GO:0044238	primary metabolic process	2	28
GO:0071704	organic substance metabolic process	2	28
GO:1901564	organonitrogen compound metabolic process	3	28

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	28
GO:0004175	endopeptidase activity	4	28
GO:0004197	cysteine-type endopeptidase activity	5	28
GO:0004198	calcium-dependent cysteine-type endopeptidase activity	6	28
GO:0008233	peptidase activity	3	28
GO:0008234	cysteine-type peptidase activity	4	28
GO:0016787	hydrolase activity	2	28
GO:0140096	catalytic activity, acting on a protein	2	28

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	447	451	PF00656	0.569
CLV_NRD_NRD_1	451	453	PF00675	0.208
CLV_PCSK_KEX2_1	504	506	PF00082	0.453
CLV_PCSK_KEX2_1	604	606	PF00082	0.312
CLV_PCSK_KEX2_1	634	636	PF00082	0.532
CLV_PCSK_PC1ET2_1	504	506	PF00082	0.432
CLV_PCSK_PC1ET2_1	604	606	PF00082	0.295
CLV_PCSK_PC1ET2_1	634	636	PF00082	0.574
CLV_PCSK_SKI1_1	133	137	PF00082	0.548
CLV_PCSK_SKI1_1	277	281	PF00082	0.205
CLV_PCSK_SKI1_1	399	403	PF00082	0.315
DEG_Kelch_Keap1_1	4	9	PF01344	0.544
DEG_SIAH_1	123	131	PF03145	0.472
DOC_PP4_FxxP_1	195	198	PF00568	0.388
DOC_USP7_UBL2_3	295	299	PF12436	0.509
DOC_USP7_UBL2_3	449	453	PF12436	0.437
DOC_WW_Pin1_4	355	360	PF00397	0.528
DOC_WW_Pin1_4	488	493	PF00397	0.329
LIG_14-3-3_CanoR_1	133	139	PF00244	0.424
LIG_14-3-3_CanoR_1	541	550	PF00244	0.421
LIG_Actin_WH2_2	262	279	PF00022	0.395
LIG_BIR_II_1	1	5	PF00653	0.536
LIG_BIR_III_2	425	429	PF00653	0.395
LIG_BRCT_BRCA1_1	599	603	PF00533	0.497
LIG_Clathr_ClatBox_1	623	627	PF01394	0.308
LIG_deltaCOP1_diTrp_1	412	417	PF00928	0.471
LIG_deltaCOP1_diTrp_1	545	549	PF00928	0.394
LIG_eIF4E_1	374	380	PF01652	0.625
LIG_FHA_1	130	136	PF00498	0.446
LIG_FHA_1	475	481	PF00498	0.522
LIG_FHA_1	494	500	PF00498	0.408
LIG_FHA_2	384	390	PF00498	0.441
LIG_FHA_2	420	426	PF00498	0.508
LIG_LIR_Apic_2	193	198	PF02991	0.198
LIG_LIR_Apic_2	91	97	PF02991	0.535
LIG_LIR_Gen_1	294	304	PF02991	0.524
LIG_LIR_Gen_1	320	327	PF02991	0.485
LIG_LIR_Gen_1	338	349	PF02991	0.491
LIG_LIR_Gen_1	372	380	PF02991	0.531
LIG_LIR_Gen_1	493	499	PF02991	0.337
LIG_LIR_Gen_1	544	555	PF02991	0.455
LIG_LIR_Gen_1	57	66	PF02991	0.455
LIG_LIR_Nem_3	209	215	PF02991	0.197
LIG_LIR_Nem_3	248	254	PF02991	0.518
LIG_LIR_Nem_3	266	271	PF02991	0.542
LIG_LIR_Nem_3	287	292	PF02991	0.497
LIG_LIR_Nem_3	294	300	PF02991	0.511
LIG_LIR_Nem_3	303	307	PF02991	0.514
LIG_LIR_Nem_3	338	344	PF02991	0.471
LIG_LIR_Nem_3	372	377	PF02991	0.534
LIG_LIR_Nem_3	493	498	PF02991	0.355
LIG_LIR_Nem_3	511	516	PF02991	0.464
LIG_LIR_Nem_3	527	532	PF02991	0.467
LIG_LIR_Nem_3	544	550	PF02991	0.482
LIG_LIR_Nem_3	57	61	PF02991	0.429
LIG_LIR_Nem_3	600	606	PF02991	0.416
LIG_LIR_Nem_3	614	619	PF02991	0.478
LIG_LIR_Nem_3	7	13	PF02991	0.690
LIG_Pex14_2	444	448	PF04695	0.542
LIG_Pex14_2	54	58	PF04695	0.488
LIG_SH2_CRK	297	301	PF00017	0.509
LIG_SH2_CRK	304	308	PF00017	0.504
LIG_SH2_CRK	352	356	PF00017	0.477
LIG_SH2_CRK	374	378	PF00017	0.509
LIG_SH2_CRK	513	517	PF00017	0.474
LIG_SH2_CRK	529	533	PF00017	0.477
LIG_SH2_CRK	94	98	PF00017	0.772
LIG_SH2_GRB2like	454	457	PF00017	0.437
LIG_SH2_NCK_1	374	378	PF00017	0.493
LIG_SH2_NCK_1	94	98	PF00017	0.552
LIG_SH2_PTP2	566	569	PF00017	0.390
LIG_SH2_SRC	268	271	PF00017	0.539
LIG_SH2_SRC	392	395	PF00017	0.538
LIG_SH2_STAP1	221	225	PF00017	0.210
LIG_SH2_STAP1	278	282	PF00017	0.562
LIG_SH2_STAP1	468	472	PF00017	0.357
LIG_SH2_STAP1	64	68	PF00017	0.401
LIG_SH2_STAT3	32	35	PF00017	0.721
LIG_SH2_STAT5	251	254	PF00017	0.508
LIG_SH2_STAT5	268	271	PF00017	0.524
LIG_SH2_STAT5	352	355	PF00017	0.554
LIG_SH2_STAT5	385	388	PF00017	0.468
LIG_SH2_STAT5	392	395	PF00017	0.467
LIG_SH2_STAT5	454	457	PF00017	0.470
LIG_SH2_STAT5	566	569	PF00017	0.374
LIG_SH2_STAT5	584	587	PF00017	0.410
LIG_SH3_3	100	106	PF00018	0.529
LIG_SH3_3	486	492	PF00018	0.376
LIG_SH3_3	575	581	PF00018	0.514
LIG_SH3_3	623	629	PF00018	0.474
LIG_SUMO_SIM_anti_2	554	560	PF11976	0.232
LIG_SUMO_SIM_par_1	482	488	PF11976	0.411
LIG_SUMO_SIM_par_1	514	519	PF11976	0.427
LIG_TRAF2_1	33	36	PF00917	0.680
LIG_TRAF2_1	41	44	PF00917	0.536
LIG_TRAF2_1	77	80	PF00917	0.535
LIG_TRAF2_1	82	85	PF00917	0.534
LIG_TYR_ITIM	200	205	PF00017	0.197
LIG_UBA3_1	200	208	PF00899	0.197
LIG_UBA3_1	443	449	PF00899	0.395
LIG_UBA3_1	557	565	PF00899	0.251
LIG_WRC_WIRS_1	251	256	PF05994	0.465
MOD_CDK_SPxK_1	355	361	PF00069	0.553
MOD_CK2_1	149	155	PF00069	0.463
MOD_CK2_1	30	36	PF00069	0.562
MOD_CK2_1	383	389	PF00069	0.473
MOD_CK2_1	516	522	PF00069	0.389
MOD_CMANNOS	445	448	PF00535	0.293
MOD_GlcNHglycan	107	110	PF01048	0.677
MOD_GlcNHglycan	124	127	PF01048	0.727
MOD_GlcNHglycan	271	274	PF01048	0.351
MOD_GlcNHglycan	280	283	PF01048	0.349
MOD_GlcNHglycan	308	311	PF01048	0.286
MOD_GlcNHglycan	412	416	PF01048	0.362
MOD_GlcNHglycan	481	484	PF01048	0.415
MOD_GlcNHglycan	535	538	PF01048	0.428
MOD_GlcNHglycan	64	67	PF01048	0.612
MOD_GSK3_1	317	324	PF00069	0.513
MOD_GSK3_1	350	357	PF00069	0.528
MOD_GSK3_1	407	414	PF00069	0.462
MOD_GSK3_1	493	500	PF00069	0.396
MOD_GSK3_1	541	548	PF00069	0.488
MOD_N-GLC_1	215	220	PF02516	0.197
MOD_N-GLC_1	239	244	PF02516	0.432
MOD_N-GLC_1	508	513	PF02516	0.336
MOD_N-GLC_1	533	538	PF02516	0.396
MOD_NEK2_1	156	161	PF00069	0.449
MOD_NEK2_1	238	243	PF00069	0.376
MOD_NEK2_1	300	305	PF00069	0.517
MOD_NEK2_1	327	332	PF00069	0.525
MOD_NEK2_1	444	449	PF00069	0.501
MOD_NEK2_1	533	538	PF00069	0.362
MOD_NEK2_2	321	326	PF00069	0.395
MOD_NEK2_2	597	602	PF00069	0.281
MOD_PIKK_1	31	37	PF00454	0.659
MOD_PIKK_1	497	503	PF00454	0.449
MOD_PIKK_1	641	647	PF00454	0.429
MOD_PKA_2	540	546	PF00069	0.505
MOD_Plk_1	175	181	PF00069	0.438
MOD_Plk_1	215	221	PF00069	0.327
MOD_Plk_1	238	244	PF00069	0.270
MOD_Plk_1	25	31	PF00069	0.611
MOD_Plk_1	327	333	PF00069	0.498
MOD_Plk_1	553	559	PF00069	0.350
MOD_Plk_1	597	603	PF00069	0.353
MOD_Plk_2-3	329	335	PF00069	0.395
MOD_Plk_4	175	181	PF00069	0.703
MOD_Plk_4	264	270	PF00069	0.515
MOD_Plk_4	350	356	PF00069	0.557
MOD_Plk_4	383	389	PF00069	0.523
MOD_Plk_4	553	559	PF00069	0.401
MOD_ProDKin_1	355	361	PF00069	0.528
MOD_ProDKin_1	488	494	PF00069	0.325
MOD_SUMO_for_1	187	190	PF00179	0.197
MOD_SUMO_for_1	617	620	PF00179	0.329
MOD_SUMO_rev_2	328	337	PF00179	0.478
MOD_SUMO_rev_2	389	398	PF00179	0.511
MOD_SUMO_rev_2	511	516	PF00179	0.505
MOD_SUMO_rev_2	65	70	PF00179	0.633
TRG_DiLeu_BaEn_4	15	21	PF01217	0.547
TRG_ENDOCYTIC_2	202	205	PF00928	0.197
TRG_ENDOCYTIC_2	251	254	PF00928	0.537
TRG_ENDOCYTIC_2	268	271	PF00928	0.540
TRG_ENDOCYTIC_2	297	300	PF00928	0.510
TRG_ENDOCYTIC_2	304	307	PF00928	0.506
TRG_ENDOCYTIC_2	352	355	PF00928	0.479
TRG_ENDOCYTIC_2	374	377	PF00928	0.525
TRG_ENDOCYTIC_2	385	388	PF00928	0.533
TRG_ENDOCYTIC_2	513	516	PF00928	0.428
TRG_ENDOCYTIC_2	529	532	PF00928	0.429
TRG_ENDOCYTIC_2	566	569	PF00928	0.368
TRG_ER_diArg_1	130	133	PF00400	0.678
TRG_Pf-PMV_PEXEL_1	11	15	PF00026	0.551

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8T6	Leptomonas seymouri	35%	93%
A0A0N1I8N2	Leptomonas seymouri	29%	66%
A0A0N1IGQ2	Leptomonas seymouri	34%	92%
A0A0N1P9P1	Leptomonas seymouri	36%	76%
A0A0N1PCA9	Leptomonas seymouri	34%	88%
A0A0N1PE91	Leptomonas seymouri	24%	73%
A0A1X0NJK2	Trypanosomatidae	31%	86%
A0A1X0NMT3	Trypanosomatidae	40%	76%
A0A1X0NWA6	Trypanosomatidae	30%	75%
A0A3S7WW41	Leishmania donovani	35%	88%
A0A3S7X463	Leishmania donovani	26%	70%
A4H3W4	Leishmania braziliensis	36%	76%
A4HE81	Leishmania braziliensis	35%	92%
A4HJ22	Leishmania braziliensis	31%	69%
A4HJ23	Leishmania braziliensis	38%	100%
A4HYW3	Leishmania infantum	35%	88%
A4I6K5	Leishmania infantum	26%	70%
A8MX76	Homo sapiens	28%	95%
C9ZY36	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	36%	76%
E8NHF1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
E8NHG7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	69%
E8NHM8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
E8NHQ2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	37%	100%
E9AIH4	Leishmania braziliensis	35%	88%
E9AIH6	Leishmania braziliensis	27%	100%
E9AK26	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	82%
E9AUR0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	88%
E9AUR1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	100%
E9B1J1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	70%
P27730	Schistosoma mansoni	24%	86%
Q4Q6L7	Leishmania major	36%	89%
Q4Q6M0	Leishmania major	29%	69%
Q4Q6M2	Leishmania major	23%	86%
Q4Q6M3	Leishmania major	27%	70%
Q4QCS6	Leishmania major	36%	88%
V5D9Y2	Trypanosoma cruzi	36%	77%